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去岩藻糖GPC3抗体增强NK92细胞对肝癌细胞的杀伤效果
Authors
岳萍
曾柱
+4 more
游敏
邱炜
陈琳
黄文竹
Publication date
1 December 2019
Publisher
Doi
Cite
Abstract
目的去岩藻糖基化改造抗磷脂酰肌醇蛋白聚糖3(Glypican-3,GPC3)抗体GC33,并研究该抗体依赖的NK92细胞对肝癌细胞的杀伤效果。方法以岩藻糖基转移酶8(fucosyltransferase 8,Fut8)缺陷的中国仓鼠卵巢癌细胞(Chinese hamster ovary,CHO)表达去岩藻糖基化的GC33抗体,以野生型CHO细胞表达的GC33作为对照,通过梯度酶联免疫反应检测其与GPC3及FC gama receptorⅢa(FCGRⅢa)的结合活性;以NK92细胞作为效应细胞,检测去岩藻糖基化GC33抗体依赖的NK92细胞对GPC3阳性的肝癌细胞Hep G2和Huh 7细胞毒作用。结果去岩藻糖基化改造的GC33抗体保留了与野生型GC33抗体对GPC3相当的结合活性,其与FCGRⅢa的结合能力增强了7倍,增强天然杀伤细胞(natural killer cells,NK)对Hep G2和Huh 7细胞的细胞毒作用(antibody dependent cellular cytotoxicity,ADCC)。结论去岩藻糖基化增强了GC33抗体依赖的NK92细胞对肝癌细胞杀伤效果
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Last time updated on 20/11/2020