校园网络使用特点以及相关管理要求

中国教育和科研计算机网CErnET始建于1994年,是由国家投资建设,教育部负责管理,清华大学等高等学校承担建设和运行的全国性学术计算机互联网络,是全国最大的公益性计算机互联网络。随着国家对教育投入的不断增加和高

Cloning and Analysis of the AmphiPax1/9 Upstream Regulatory Region

脊椎动物PAX1/9是一重要的发育调控基因亚家族,文昌鱼基因组中仅有单一的该亚家族直系同源基因AMPHI-PAX1/9,为检验此基因上游调控元件的功能在脊椎动物体内是否具有通用性,将文昌鱼PAX1/9基因上游约4.6 kb的侧翼序列与绿色荧光蛋白(gfP)报告基因连接,构建重组质粒表达载体(PAMPHIPAX1/9-ACgfP),显微注射斑马鱼胚胎,并以斑马鱼PAX1和PAX9的上游同源序列为阳性对照.结果表明,阳性对照斑马鱼胚胎中gfP能够表达,但注射PAM-PHIPAX1/9-ACgfP质粒的斑马鱼胚胎中无明显的gfP表达,这一现象可能是由于PAX1/9上游调控序列在二物种间存在较大的进化差异,启动元件具有物种特异性.Pax1/9 is a crucial developmental regulatory gene subfamily in vertebrates.Amphioxus possesses a single orthologous gene AmphiPax1/9 of the subfamily.In order to investigate if the AmphiPax1/9 upstream sequence contains regulatory elements and promotes gene expression in zebrafish,a recombinant expression vectors(pAmphiPax1/9-AcGFP) inserted with a 4.6 kb DNA segment of AmphiPax1/9 upstream region was constructed,and then,microinjected the vectors into zebrafish embryos at one-cell stage using homologous sequences from zebrafish Pax1 and Pax9 as positive controls.The results showed that no GFP expression is observable in the embryos injected with pAmphiPax1/9-AcGFP,whereas the expression appears clearly in the positive controls.The observations suggest that the AmphiPax1/9 upstream region was unable to promote gene expression in zebrafish,which might be owing to the deep divergence between the two species.国家自然科学基金重点项目(30830023);教育部高校博士学科点专项科研基金(20070384041

Magnetic Water Improve the Tolerance of Corn to Cd Ion

本工作在实验室条件下研究磁化水对玉米耐受重金属镉的影响.测定了几种抗氧化酶的活性,与对照组相比,磁化水处理的植株叶片超氧化物歧化酶和过氧化氢酶活性提高,丙二醛含量减少.因此提出,在重金属镉的胁迫下,磁化水可通过提高超氧化物歧化酶和过氧化氢酶活性,消除镉引发的氧化胁迫,保护了细胞膜的完整性(具体体现为植株体内丙二醛含量较低),从而增强植株对重金属镉的耐受性,使植株免受或减缓伤害.对我国受Cd污染地区玉米的种植具有一定的指导意义.In this study,we investigated the effect of magnetic water to the Cd-induced corn's phenotype in the laboratory.In mechanism,the corn flooded by magnetic water display higher SOD and CAT activities and lower concentration of MDA than the control corn.Thus we concluded that Cd can induced oxidant stress in normal conditions,however,flooding of magnetic water increased the SOD and CAT activities,from this result,the concentration of MDA can be decreased in vivo,and the tolerance to the metal pollution can be improved.These conclusions have significant value for culture of corn and other crop in metal polluted area.国家基础科学人才培养基金项目(J0630649

Nusap1 is essential for neural crest cell migration in zebrafish

National Nature Science Foundation of China [30900748]; Natural Science Foundation in Fujian province [2009J01194]; Chinese National Basic Research Programs [2006CB910802]Microtubules play important roles in mitotic spindle assembly and chromosome segregation to maintain normal cell cycle progression. A number of microtubule-associated proteins have been identified in epithelial and neural cell cultures; however, their physiological significance is not well characterized due to the lack of appropriate in vivo animal models. Nucleolar spindle-associated protein (NuSAP) is a microtubule-binding protein and is reported to be involved in mitosis by cell culture studies. In this report, we identified the zebrafish homologue of human NuSAP and investigated its expression profile and functions. Using in situ hybridization, we demonstrated that transcripts of zebrafish nusap1 are specifically expressed in the retina, forebrain, hindbrain and neural crest. When the in vivo expression of nusap1 was knocked down through antisense oligonucleotide morpholino technology, the morphants of nusap1 showed impaired morphogenesis in the trunk and yolk extension, implying the involvement of Nusap1 in cell migration. Mechanistic studies revealed that nusap1 morphants have an altered expression pattern of neural crest markers crestin and sox9b, but normal expression of blood vessel and notochord markers gata1 and shh. In addition, nusap1 mRNA injection caused serious apoptosis in retina and hindbrain tissue, and these phenotypes can be rescued by co-injection of morpholino against nusap1. These observations not only suggest a role for Nusap1 in connecting apoptosis with cell migration, but also provide strong evidences that Nusap1 is potentially involved in morphogenesis in vertebrates

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies