Study on the prothymosin α as vaccine adjuvant of P.yoelii

目的:研究胸腺素α原(PrOTHyMOSInα,PrOTα)作为约氏疟原虫疫苗免疫佐剂的作用。方法:提取P.yOElII-17Xnl全蛋白作为抗原,用胸腺素α原作为免疫佐剂,免疫小鼠。具体方案为:昆明小鼠分为4组,每组6只,A组免疫P.yOElII-17Xnl全蛋白+PrOTα;b组免疫P.yOElII-17Xnl全蛋白;C组只注射PrOTα;d组为空白对照,以相同体积的生理盐水代替。免疫结束后感染致死的P.yOElII-17Xl,1x107个虫/只小鼠。结果:感染后的前10天A组小鼠疟原虫血症平均值要低于其他三组,且最终有3只小鼠存活下来,存活率50%,C组有一只小鼠存活,b、d组小鼠全部死亡。结论:用P.yOElII-17Xnl全蛋白做抗原,用PrOTα作为佐剂,比单独用P.yOElII-17Xnl全蛋白对小鼠有更好的免疫保护作用,提示了PrOTα可以成为一种有潜力的蛋白疫苗。Objective:To investigate the function of prothymosin α(ProTα) as vaccine adjuvant of P.yoelii.Methods:The mice were immunized with the total protein extracted from P.yoelii-17XNL as antigen,together with prothymosin α as adjuvant.Programs:Kunming mice were divided into A,B,C and D group.A group was immunized with P.yoelii-17XNL total protein and ProTα;B group was immunized with P.yoelii-17XNL;C group was only injected with ProTα;D group was the control,only injected with physiological saline.And then,the mice of each group was infected with P.yoelii-17XL,the dosage was 1×107/mice.Results:The parasitemia of A group-mice was lower than the other three groups in the first 10 days after infection,and eventually there were three mice survived in A group,the survival rate was 50%,one mouse survived in C group,all of mice in B group and D group died.Conclusion:Mice immunized with P.yoelii-17XNL total protein as antigen together with ProTα as adjuvants,had better immune protection than those immunized with P.yoelii-17XNL protein only.The present results suggest that the ProTα can act as a potential adjuvant in protein vaccine.厦门市科技项目(3502Z20083004);国家“973”项目(2007CB513103)资

汽爆耦合同步糖化发酵工艺对麦麸粉理化指标及感官品质的影响

研究汽爆(steam explosion,SE)耦合同步糖化发酵(simultaneous saccharification and fermentation,SSF)新工艺对麦麸粉理化指标及感官评价的影响。汽爆麦麸粉是以蒸汽为汽爆介质,条件为0. 8 MPa维压时间5 min,处理后的麦麸与纤维素/淀粉酶和乳酸菌/酵母进行组合预处理,分别对不同工艺下麦麸的理化指标和感官品质进行分析。结果表明,经过SCL组合预处理后麦麸粉的苦味值最低(22. 9±2. 89),酸度(0. 52±0. 046) mol/L,p H值4. 64,酸味适中;∣△E∣值达到最小为(25. 6±2. 58),色泽为深红棕色,接近咖啡色;感官总分达到最高,变异系数最低,其数值分别为8. 62分和8. 78%。SCL组合预处理后麦麸的理化指标和感官品质得到了显著提高和改善,该结果对于麦麸在食品行业的应用和开发具有一定的指导意义

汽爆耦合同步糖化发酵工艺对麦麸粉理化指标及感官品质的影响

研究汽爆(steam explosion,SE)耦合同步糖化发酵(simultaneous saccharification and fermentation,SSF)新工艺对麦麸粉理化指标及感官评价的影响。汽爆麦麸粉是以蒸汽为汽爆介质,条件为0. 8 MPa维压时间5 min,处理后的麦麸与纤维素/淀粉酶和乳酸菌/酵母进行组合预处理,分别对不同工艺下麦麸的理化指标和感官品质进行分析。结果表明,经过SCL组合预处理后麦麸粉的苦味值最低(22. 9±2. 89),酸度(0. 52±0. 046) mol/L,p H值4. 64,酸味适中;∣△E∣值达到最小为(25. 6±2. 58),色泽为深红棕色,接近咖啡色;感官总分达到最高,变异系数最低,其数值分别为8. 62分和8. 78%。SCL组合预处理后麦麸的理化指标和感官品质得到了显著提高和改善,该结果对于麦麸在食品行业的应用和开发具有一定的指导意义

汽爆耦合同步糖化发酵工艺对麦麸粉理化指标及感官品质的影响

研究汽爆(steam explosion,SE)耦合同步糖化发酵(simultaneous saccharification and fermentation,SSF)新工艺对麦麸粉理化指标及感官评价的影响。汽爆麦麸粉是以蒸汽为汽爆介质,条件为0. 8 MPa维压时间5 min,处理后的麦麸与纤维素/淀粉酶和乳酸菌/酵母进行组合预处理,分别对不同工艺下麦麸的理化指标和感官品质进行分析。结果表明,经过SCL组合预处理后麦麸粉的苦味值最低(22. 9±2. 89),酸度(0. 52±0. 046) mol/L,p H值4. 64,酸味适中;∣△E∣值达到最小为(25. 6±2. 58),色泽为深红棕色,接近咖啡色;感官总分达到最高,变异系数最低,其数值分别为8. 62分和8. 78%。SCL组合预处理后麦麸的理化指标和感官品质得到了显著提高和改善,该结果对于麦麸在食品行业的应用和开发具有一定的指导意义

功能性红曲的汽爆灭菌工艺初步研究

目的:初步研究汽爆功能性红曲的灭菌效果以及对其活性物质莫纳可林K(Monacolin K)的影响。方法:汽爆功能性红曲以蒸汽为汽爆介质,研究115、125、135 ℃温度下分别维压0、1、3、5 min,功能性红曲中Monacolin K含量、水分舍量、菌落总数的变化,采用灭菌指示胶带图像处理法评价灭菌效果。结果:汽爆灭菌在达到灭菌要求的情况下 (135 ℃汽爆3 min),相比较传统高压蒸汽灭菌条件121 ℃、20 min,汽爆后功能性红曲含水量和Monacolin K的损失率分别为13.07%和3.92%,均低于传统高压蒸汽灭菌后的含水量(18.49%)和损失率(17.76%),该汽爆条件下灭菌指示胶带热敏区域灰度平均值为62.77。结论:汽爆灭菌对于功能性红曲是可行且高效的,该汽爆工艺节省红曲产品后续干燥的能量,降低了生产成本,为功能性红曲有效灭菌、保留活性成分以及减少灭菌后成品含水量的汽爆灭菌提供理论依据

功能性红曲的汽爆灭菌工艺初步研究

目的:初步研究汽爆功能性红曲的灭菌效果以及对其活性物质莫纳可林K(Monacolin K)的影响。方法:汽爆功能性红曲以蒸汽为汽爆介质,研究115、125、135℃温度下分别维压0、1、3、5 min,功能性红曲中Monacolin K含量、水分含量、菌落总数的变化,采用灭菌指示胶带图像处理法评价灭菌效果。结果:汽爆灭菌在达到灭菌要求的情况下(135℃汽爆3 min),相比较传统高压蒸汽灭菌条件121℃、20 min,汽爆后功能性红曲含水量和Monacolin K的损失率分别为13.07%和3.92%,均低于传统高压蒸汽灭菌后的含水量(18.49%)和损失率(17.76%),该汽爆条件下灭菌指示胶带热敏区域灰度平均值为62.77。结论:汽爆灭菌对于功能性红曲是可行且高效的,该汽爆工艺节省红曲产品后续干燥的能量,降低了生产成本,为功能性红曲有效灭菌、保留活性成分以及减少灭菌后成品含水量的汽爆灭菌提供理论依据。</p

A Feasibility Study on the Policy of Public Property Management Outsourcing to the Property Management Professionals

Outsourcing partial works of civil service released from the government to professional firms in the private business sector has become not only an important instrument for the development strategy for the government reforming but also a concept, shared by both the official and private sectors, for pursuing economic development and administrative reinvention. It stems fundamentally from considerations of (1) adjusting the functional capacity and role of the government so as to revitalize civil service manpower; (2) alleviating financial burden of the government to reinstate people？？s confidence in it; and (3) expanding participation of resources from the private sector to raise the efficiency and quality of the civil service. The objective of this study includes (1) evaluating the necessity of proposing a policy of public property management outsourcing to property management professionals; (2) defining the scope of public property and investigating related outsourcing methods; and (3) gathering valuable information from abroad as references for the future policy making. The contents of this study are composed of (1) integrating the methods and practices of the public property management; (2) gathering policies, regulations, and executed results of the public property management from experienced countries; (3) analyzing advantages and disadvantages to outsourcing management of Taiwan public property; (4) investigating the effects of outsourcing management of the public property on the property management industry. By literature review, fieldworks, conferences, and questionnaire as a whole, examinations in the theoretical aspect are made in this study and pragmatic conclusions and suggestions are proposed via information gathering, assorting, and analyzing. The expected results of this study are: (1) defining the scope of public property suitable for outsourcing with the requisite criteria; (2) being able to promote service quality of the domestic property management industry by properly outsourcing management of the public property to it as an incentive so as to facilitate its developments; (3) serving as advices for strategy-proposing led by the Council for Economic Planning and Development; (4) providing suggestions, with reference to experiences from abroad, for the future development of the public property management; (5) delineating the effects of outsourcing management of the public property on the property management industry; (6) presenting suggestions with regard to making use of outsourcing management of the public property as a means of promoting economic developments and enhancing the employment rate.[[abstract]]政府將部分業務委託民間辦理已成為政府再造發展策略的重要工具，也已成為政府與民間尋求經濟發展與行政改革的共同理念，其思考之核心動力在於：(1)調整政府職能與角色，活化公務人力資源；(2)改善政府財政負擔，建立人民對政府的信心；(3)擴大民間資源參與，提昇公共服務效率與品質。 本計畫研究目的為：(1)評估「研議公部門物業委託專業物業管理公司管理的政策」之必要性；(2)定義公部門物業、研究相關之委託方式；同時(3)蒐集各國資料作為未來政策研擬之參考。 本計畫研究內容包括：(1)彙整我國公部門物業管理方式及實際辦理情形；(2)蒐集各國公部門物業管理政策、法規及執行成果；(3)分析我國公部門物業管理釋出的優缺點；(4)研究公部門物業管理業務釋出對物業管理服務業之影響。 本計畫所採用之研究方法包括：文獻探討、實地訪談、座談討論與問卷調查等四種，除進行理論探討外，亦經由資訊之蒐集、彙整與分析，提出實務上可行且具實用價值之結論與建議。 本計畫之預期成果為：(1)界定公部門物業可進行委託管理之範圍及應有之原則；(2)藉由公部門物業管理相關業務之適當釋出，作為帶動我國物業管理服務業提昇品質之誘因，促進物業管理服務業之發展；(3)研究結果可作為經濟建設委員會策略研提之建議；(4)引用國外經驗，提供國內公部門物業管理未來發展建議；(5)瞭解公部門物業委外對物業管理服務業之影響；(6)研提如何運用公部門物業委外來促進經濟發展及增加就業機會