The mechanism study of the membrane lipid raft gene flotillins in the process of pathogen infection in Scylla paramamosain

拟穴青蟹（Scyllaparamamosain）是我国东南沿海分布的重要海水养殖蟹类之一，具有高的营养价值和经济价值。集约化的养殖模式导致近年来拟穴青蟹疾病频发，而细菌性病原，特别是以弧菌等革兰氏阴性菌为主的病原是青蟹发病的重要诱因，对其致病入胞途径和机制的研究能够为控制拟穴青蟹疾病爆发提供科学依据。前期的研究表明青蟹感染溶藻弧菌3h后，SpFLT-1mRNA在血淋巴细胞和鳃中显著上调表达，且该蛋白分布于青蟹血淋巴细胞和鳃上皮细胞的膜脂筏结构中，与弧菌蛋白之间发生共定位，暗示它们之间可能存在相互作用。本研究在前期工作基础上，改进了RNAi技术的有效性，并构建了SpFLT-1蛋白在细胞中的过表达...The mud crab, Scylla paramamosain is one of the most important marine breeding crabs in China, with vital nutritional and economic value. The animals are usually raised in ponds at high densities which has led to disease epidemics in recent years. Bacteria especially Gram-negative bacteria (e.g. Vibrios), are one of the leading pathogens infecting these crabs. However, the pathogenesis is poorly u...学位：博士后院系专业：海洋与地球学院_海洋生物学学号：201317004

Cloning, expression and characterization of membrane lipid rafts related gene SpFLT-1 and its functional study during pathogens endocytosis in Scylla paramamosain

拟穴青蟹(Scyllaparamamosain)是我国最重要的海水养殖蟹类之一，具有重要的营养价值和经济价值。集约化的养殖模式导致了近年来拟穴青蟹的疾病不断爆发，其中以弧菌等革兰氏阴性菌为主的细菌性病原是导致青蟹发病的重要诱因之一。然而由于对病原微生物感染拟穴青蟹的致病机理知之甚少，目前对其尚无有效的免疫防治措施。因此，阐明病原微生物的致病途径对于控制拟穴青蟹疾病爆发具有重要的意义。 病原微生物在长期的进化过程中发展出一整套逃避、干扰、抑制和对抗宿主免疫系统的机制，其中利用宿主的细胞膜结构和受体蛋白进入胞内是病原微生物感染宿主的重要途径之一。为了研究病原在入侵拟穴青蟹过程中与宿主作用的关键蛋...The mud crab, Scylla paramamosain is one of the most important marine breeding crabs in China, with vital nutritional and economic value. The animals are usually raised in ponds at high densities which has led to disease epidemics in recent years. Bacteria especially Gram-negative bacteria (e.g. Vibrios), are one of the leading pathogens infecting these crabs. However, the pathogenesis is poorly u...学位：理学博士院系专业：环境与生态学院_环境科学学号：2262009015327

On the English Translation of Chinese Film Titles From the Perspective of Skopostheorie

在当今这个全球化的时代，电影已成为跨文化交际的一个重要形式。通过观看外国电影，可以迅速地对另一种文化产生一定程度的了解。随着中国电影越来越引起世界的关注，中国电影开始走出国门，走向世界，因此中国电影片名的翻译就变得十分重要了，优秀的译名能跨越文化障碍，更好地宣传影片，促进中华文化的传播和交流。要想使中国电影得到不同文化范围内观众的欣赏，研究中文电影片名英译的翻译策略已成为一种必然，这是时代赋予我们的责任。 电影片名翻译是一种语言转换，也是一种商业行为，其目的在于追求市场效益和商业利润，尽最大可能争取吸引眼球，打动潜在观众，促成其观赏行动。以目的论来指导电影片名翻译，其优势在于能够更加深入地理...In the present age of globalization, film is an important form in cross-cultural communication. By watching foreign films, a certain degree of understanding of another culture can be gained. With Chinese films getting more and more attention, Chinese films are introduced into the outside world, therefore, the translation of Chinese film titles is of great importance. Excellent translated film titl...学位：翻译硕士院系专业：外文学院英语语言文学系_翻译硕士学号：X200811000

海洋生物响应海洋酸化研究进展

海洋酸化是大气CO2浓度持续增加引起的又一环境问题,近些年来已经成为科学研究的热点问题并受到社会的广泛关注。该文结合国内外相关研究进展,简要概述了海洋酸化作用对海洋生物的营养组成、感觉系统、胚胎和幼体发育等的影响,以及在复杂的海洋生态系统中,海洋酸化与低氧、环境污染物的相互作用及其对生物的影响,旨在为深入研究多重环境胁迫对海洋生物的作用提供参考

拟穴青蟹抗菌肽Crustin新变体的表达特性与抗菌功能

Crustins是一类较早发现并广泛分布在甲壳动物中且富含半胱氨酸的阳离子抗菌肽,能够参与抗细菌的免疫应答。从拟穴青蟹（Scylla paramamosain）中克隆获得Crustin基因,命名为SpCrus1a,其cDNA序列全长744 bp,开放阅读框编码113个氨基酸,成熟肽分子量10.03 kDa,等电点8.30。表达分析结果发现其转录本主要存在于鳃、卵巢、上皮组织中,脂多糖刺激后SpCrus1a会上调表达。体外合成SpCrus1a的第29～47位氨基酸进行抗菌活性实验发现,其对革兰氏阳性菌具有较强的抗菌活性,对被测的革兰氏阴性菌抗菌活性较弱或无抗菌活性。合成肽Sp Crus1a29-47能够在较短时间内杀死绝大多数的金黄色葡萄球菌,2 h内杀死全部的金黄色葡萄球菌。扫描电镜发现合成肽Sp Crus1a29-47可造成金黄色葡萄球菌表面结构崎岖不平,高浓度Sp Crus1a29-47造成细菌的大量死亡。上述结果表明,SpCrus1a是抗菌肽Crustins的新变体。国家自然科学基金(U205123,31172438);;厦门南方海洋研究中心科技项目(14PYY050SF03

The Effects of Benzo [a] Pyrene (BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream(Pagrus major)

通过水体暴露方式对海水养殖真鲷进行bAP持续染毒,利用实时定量PCr技术研究了真鲷细胞色素P450基因(CyP1A1)和芳香烃受体基因(AHr2)随bAP暴露剂量、时间的动力学变化。结果发现,0.1~1.5μg/l环境浓度的bAP能够显著性诱导CyP1A1基因和AHr2基因的表达,且AHr2 MrnA早于CyP1A1 MrnA被诱导表达;bAP持续暴露48 H,CyP1A1和AHr2基因的表达水平均随暴露时间的延长而显著升高,染毒72 H后又回复到本底水平,实验表明这两个基因的表达与bAP的暴露剂量和暴露时间之间具有显著性的剂量-效应和时间-效应关系。The gene expression patterns of CYP1A1 and Aryl hydrocarbon receptor(AhR2) of red seabream(Pagrus major) were both measured using real-time quantitative PCR(qPCR) when fish exposed to environmentally relevant concentration of BaP(0.1,0.5 and 1.0 μg/L,respectively).The results showed that CYP1A1 mRNA and AhR2 mRNA could be induced significantly,besides,the time of AhR2 mRNA induced ahead of the time of CYP1A mRNA induced.The two genes were induced markedly at the begining of BaP exposure,and then decreased to the basal levels after 72 h.The results demonstrate that BaP can regulate CYP1A1 and AhR2 transcript in a dose and time dependent manner.国家“863”计划重点资助项目(2007AA091406);国家自然科学基金资助项目(30770391

Trial production and industrial sidetrack test of the XH-1 type of potassium-free sulfur-tolerant shift catalyst

作者简介: 连奕新( 1971-),男,博士生;杨意泉(1944- ),男, 硕士,研究员,博士生导师,主要从事工业催化方面的研究, 通讯联系人,0592-2186368, [email protected]。[中文文摘]以镁铝尖晶石复合材料为载体,采用浸渍钴钼活性组分无煅烧技术,中试制备出新型XH-1无钾耐硫变换催化剂。对该催化剂进行实验室活性评价和6个月的工业侧线试验,结果表明:XH-1型催化剂结构稳定、机械强度高;在中压(2.0MPa)、低汽气比(0.3~0.6)的反应条件下,催化活性优于国内外同类产品;在高压(≥3.0 MPa)、高汽气比(≥1.0)条件下催化性能与同类产品相当,符合高、中压各种变换工艺条件对催化剂性能的要求。[英文文摘]New XH-1 type of potassium-free sulfur-tolerant water gas shift catalyst was trial produced on a pilot scale by the impregnation of Co-Mo active composition on the complex support of magnalium-alumina spinel without calcination.The results of both the evaluation in the laboratory and the industrial sidetrack test in a period of six months showed that the XH-1 type of catalyst had higher activity in comparison with the same type of products made in inland and overseas under medium pressure(2.0 MPa) and at ow steamtogas ratio( 0.3-0.6),but the same activity under high pressure (3.0MPa) and at high steamtogas ratio(≥1.0).Moreover the XH-1 type of catalyst was found to have stable construction and high mechanical strength. The catalyst was suitable for different shift technology conditions of high and medium pressure.厦门市企业创新基金计划项目(3502Z20031082

Antioxidant enzymes from the crab Scylla paramamosain: Gene cloning and gene/protein expression profiles against LPS challenge

Recent studies revealed that antioxidant enzymes play important roles in antioxidant responses caused by metabolic process or pathogen invasion. Catalase is one of these key enzymes which has been characterized and highly conserved from invertebrates to vertebrates. In the present study, a full-length cDNA sequence of catalase was cloned from the hemocyte suppression subtractive hybridization library of the crab Scylla paramamosain. The Sp-catalase (Sp-CAT) cDNA sequence contained 2551 bp with an open reading frame of 1551 bp encoding 517 amino acid residues. The conserved catalytic active residues His-71, Asn-144 and Tyr-354 were predicted in the amino acid sequence of Sp-CAT. The deduced Sp-CAT protein had a calculated molecular mass of 59 kDa with an estimated isoelectric point of 6.4. Multiple alignment analysis revealed that the deduced amino acid sequence of Sp-CAT shared high identity (75.4%) with those of other species. The Sp-CAT mRNA transcripts were demonstrated in multiple tissues of normal S. paramamosain. After LPS challenge, the expression level of Sp-CAT gene was increased significantly in hemocyte at 3 and 6 h, and in hepatopancreas at 6 h, respectively, determined by quantitative real-time PCR. Furthermore, the activities of CAT and SOD were also measured in different tissues and serum after LPS challenge. The CAT activity was significantly increased at 3, 6, 24 and 48 h in hemocyte lysate, at 3 h in serum, and at 24 and 48 h in hepatopancreas after LPS challenge. In addition, the SOD activity was significantly induced at 3 and 6 h in hemocyte lysate, 3 and 12 h in serum, 12 and 48 h in hepatopancreas post LPS stimulation, indicating a tissue and time-dependent antioxidant response in the crab. Taken together, these data demonstrated that a strong antioxidant response occurred in the LPS-challenged crab, which might be involved in the protection of host against microbial infections. (C) 2010 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China (NSFC) [40676083]; National High Technology Research and Development Program of China [2007AA091406]; Minjiang Scholars Program [2009]; Natural Science Foundation of Fujian Province China (NSFC-FPC) [2009J05084

Identification of genes differentially expressed in hemocytes of Scylla paramamosain in response to lipopolysaccharide

Although the crab Scylla paramamosain has been cultured in China for a long time, little knowledge is available on how crabs respond to infection by bacteria. A forward suppression subtractive hybridization (SSH) cDNA library was constructed from their hemocytes and the up-regulated genes were identified in order to isolate differentially expressed genes in S. paramamosain in response to bacterial lipopolysaccharide (LPS). A total of 721 clones on the middle scale in the SSH library were sequenced. Among these genes, 271 potentially functional genes were recognized based on the BLAST searches in NCBI and were categorized into seven groups in association with different biological processes using AmiGO against the Gene Ontology database. Of the 271 genes, 269 translatable DNA sequences were predicted to be proteins, and the putative amino acid sequences were searched for conserved domains and proteins using the CD-Search service and BLASTp. Among 271 genes, 179 (66.1%) were annotated to be involved in different biological processes, while 92 genes (33.9%) were classified as an unknown-function gene group. It was noted that only 18 of the 271 genes (6.6%) had previously been reported in other crustaceans and most of the screened genes showed less similarity to known sequences based on BLASTn results, suggesting that 253 genes were found for the first time in S. paramamosain. Furthermore, two upregulated genes screened from the SSH library were selected for full-length cDNA sequence cloning and in vivo expression study, including Sp-superoxide dismutase (Sp-Cu-ZnSOD) gene and Sp-serpin gene. The differential expression pattern of the two genes during the time course of LPS challenge was analyzed using real-time PCR. We found that both genes were significantly expressed in LPS-challenged crabs in comparison with control. Taken together, the study primarily provides the data of the upregulated genes associated with different biological processes in S. paramamosain in response to LPS, by which the interesting genes or proteins potentially involved in the innate immune defense of S. paramamosain will be investigated in future. (C) 2009 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China (NSFC) [40676083]; National High Technology Research and Development Program of China [2007AA091406]; Program for Minjiang Scholars of Fujian Provinc

Disequilibrium of natural decay series in sediments of intertidal mudflats of Xiamen

用HPGeγ谱仪测定了厦门潮间带表层沉积物中的放射性核素,得到11种核素238U2、34Th2、26Ra2、22Rn2、10Pb2、28Ra2、28Ac2、28Th2、24Ra2、12Pb、40K的平均比活度分别为:40.2、82.7、32.4、28.9、94.2、69.3、57.1、71.6、64.0、71.4、692Bq/kg.结果表明潮间带沉积物中天然放射系不平衡,其中234Th相对于238U2、10Pb相对于226Ra过剩,222Rn相对于226Ra2、28Ac相对于228Ra2、24Ra相对于228Th亏损. 【英文摘要】 The natural radionuclides ()~(238)U,()~(234)Th,()~(226)Ra,()~(222)Rn,()~(210)Pb,()~(228)Ra,()~(228)Ac,()~(228)Th,()~(224)Ra,()~(212)Pb,()~(40)K in surface sediments of intertidal mudflats of Xiamen were measured using HPGe γ spectrometer.The mean specitific activities of above 11 radionuclides are respectively 40.2,82.7,32.4,28.9,(94.2,)69.3,57.1,71.6,64.0,71.4,692 Bq/kg. The results show that natural decay series in sediments of intertidal mudflats are disequilibrium ()~(234)Th related to ()~(238)U ,()~(21...国家自然科学基金资助项目(40036010