The Micro DC Motor Testing System Based on the Spectral Analysis of Steady-state Current

微型直流电机具有结构简单、控制方便、工作稳定、制造成本低廉的特点，因而成为各种机械电子系统的关键执行部件和主要驱动部件，广泛应用工业生产和社会生活的各个领域，随着社会自动化程度的提高和现代微型直流电机制造水平的提高，微型直流电机的应用范围和产量将会不断扩大。微型直流电机是系统设备的核心部件，一旦发生故障，影响面甚广，因此必须严格保证电机的出厂质量。针对目前微型直流电机的出厂质量主要依靠人工检测的问题，本文将设计一种可以对微型直流电机进行在线检测的自动化设备。 经研究发现，微型直流电机如果在制造的过程中出现故障，其电枢的起动电流信号和稳态电流信号会出现异常。本文在详细分析永磁直流电机数学模型的...Misco DC motor has characteristics of simple structure、easy to control、working stably、low manufacturing cost,So it s widely used as critical execution unit and principal drive element in Electro-Mechanical systems, Misco DC motor covers many fields of industrial manufacture and social life. With the improvement of the degree of automation and the quality of Misco DC motor, it can expand consi...学位：工学硕士院系专业：物理与机电工程学院_精密仪器及机械学号：1992011115273

An Investigation into the Chinese Phonetic Standard Applied in Chinese Textbooks of Primary Schools

结合《汉语拼音方案》等相关语言文字规范标准,本文对国内小学教育阶段目前使用的四套语文教材汉语拼音使用和教学的情况进行调研。研究发现,国家语言文字规范标准在语文教材中并没有完全落实,小学汉语拼音教学中存在着一些不合规范的现象,如"y、w"是不是声母、韵母的数量、以及标调、拼写规则等。这些失范问题值得重视,亟需科学合理的解决措施。The paper investigates the use and teaching of Pinyin in primary schools based on four sets of Chinese textbooks,as well as language and character standards such as the Scheme for the Chinese Phonetic Alphabet. It is found that teaching of Pinyin does not fully comply with the national phonetic standard. Problems include "y"and "w"as initial consonants,the quantity of vowels and rules of pronunciation and spelling. These issues should be paid enough attention to,and scientific and reasonable solutions should be proposed

一种基于各向异性磁传感器的车辆检测方法

针对目前车辆检测方法受环境影响大、精度低、成本高和安装维护复杂等问题,文章提出一种基于各向异性磁阻效应的车辆检测方法,利用两个各向异性磁阻传感器组成三轴检测电路,通过检测车辆对地磁场的扰动来进行车辆检测。详细了介绍了地磁检测原理、硬件构成和软件设计,实验结果证明利用该地磁检测模块进行车辆检测有一定的优势

The pathogeny of ulcer disease in Epinephlus awoara

2002年夏季厦门同安湾一带气候炎热,降雨量少,海水盐度偏高,同安湾刘五店的网箱养殖石斑鱼大面积暴发溃疡病。本研究调查了石斑鱼溃疡病暴发的特征和症状,主要表现为肌肉溃疡坏死、眼球脱落、鱼骨暴露等。从病鱼体表及内脏分离出优势菌群命名为TS 628,经回归感染证实TS 628就是引发本次石斑鱼溃疡病的病原菌。对病原菌进行鉴定发现,该菌革兰氏染色呈阴性,电镜下观察菌体呈短杆状,极端单鞭毛,综合研究该菌在形态、生理生化、16SrDNA同源性及药物敏感性等方面的特性,基本确认分离到的病原菌为哈维氏弧菌(Vibrioharveyi),该菌对氯霉素、壮观霉素等多种抗生素敏感,对万古霉素、青霉素G等抗生素不敏感。哈维氏弧菌是水产养殖病害常见病原菌,但作为养殖石斑鱼的病原菌在国内属首次报道。The summer of 2002 was hot and dry in Xiamen, and the seawater salinity was higher than usual, sometimes reaching 38 in Tongan Bay. From the end of May, ulcer disease occured on a large scale in Tongan Bay. In the middle of June, a heavy rain brought about high mortality among the cultured groupers, which caused serious loss. The characteristics of ulcer disease were observed. Infected groupers displayed sluggish swimming and refused feeding, several days later, the groupers' eyes swelled and became ulcerated, the tail turned red, the scales became detached and the back muscle gradually ulcerated, then the eyes even dropped out and the muscle became so necrotic that the spine was exposed, finally the diseased fishes died. Anatomized the dead fish and found that the livers and gills were pale and the gallbladder was distended. The time course from appearance of disease signs to death lasted about a week. Dominant bacteria, which were Gram-negative and seen short rod with single polar flagellum under electron microscope, were isolated and designated TS-628. In artifical infection test all fish　of the experimental group died on the third day after injected with bacterial suspension, while all the fish in control group showed no signs till the end of another week observation. The dead grouper had pale livers and gills and small ulcerations on the caudal fin and anal fins. These were the same signs as the natural infected fishes. The re-isolate also had the same characteristics as TS-628, which proved the isolate TS-628 was the pathogenic bacteria that triggered this ulcer disease. Different methods were used to identify the pathogenic bacteria in this study. The identification result through VITEK-AMS system GNI card was that TS-628 was Burkholderia mallei, with 99% reliability. While traditional biochemical identification revealed that TS-628 exhibited relVibrio harveyi through comparison with Bergey's Manual Determinative Bacteriology. In order to confirm absolutely different results above, further researches were carried out to identify TS-628 again. So 471bp sequence of TS-62816S rRNA gene was amplify and compared with all DNA sequences (1192858 in total) in genebank (GenBank+EMBL+DDBJ+PDB), homology analyses showed that 16 sequences were picked out to have the highest similarity, with 98% identity. These 16 sequences all originated fromV. harveyV. carchiariae which also belongs to harveyi because their similarity in physiological and biological characteristics and DNA homology. According to morphological features, physiological and biochemical characteristics and 16S rRNA gene homology comparison of the bacteria, the patV. harveyi. Drug sensitivity test showed that the pathogenic bacteria were highly sensitive to chloramphenicol, actinospectcinV. harveyi is a kind of pathogenic bacterium commonly found iV. harveyi is reported as the pathogenic bacteria of cage-cultured grouper in China. And V. harveyi should be regarded as an opportunistic pathogen which has close relation to temperature and salinity and easily causes vibriosis under conditions of high temperatures and drought. Therefore, it is necessary to guard agV. harveyi vibriosis in such summer days.福建省重大科技项目资助(2002N009

日本囊对虾人工诱变子一代遗传变异的RAPD分析

以性腺成熟的日本囊对虾(Marsupenaeus japonicus)作为亲代,进行不同剂量的60Co-γ射线照射.采用RAPD技术对诱变子一代的无节幼体基因组DNA多态性进行了检测.从20个随机寡核苷酸引物共检测177个位点,其中多态位点152个,占85.9%.单个引物获得的标记为6~12个,平均8.85个,分子量在150~2 500 bp之间.遗传相似系数用Ne i的计算方法进行计算,遗传距离则用Lynch的计算方法进行计算.实验数据表明:诱变子代与正常对照组相比,遗传多样性水平较高,诱变产生了较为显著的变异.研究结果证实:在对虾大规模养殖,种质质量严重退化的情况下,人工诱变能够促进基因组更加广泛的变异,为新品种的选育打下坚实的基础;同时证明RAPD技术在检测遗传差异方面具有较高的灵敏度和分辨率

MODIFIED METHODS FOR DNA EXTRACTION FROM STRELITZIA REGINAE BANKS

由于鹤望兰组织内含有大量多糖类及多酚类物质 ,严重干扰DNA的抽提 ,利用常规的SDS法、CTAB法和高盐低pH值法都难以抽提出高质量的鹤望兰基因组DNA .本文报道了在进行若干预处理之后 ,再用常规的SDS、CTAB、SDS裂解的高盐低pH值法和CTAB裂解的高盐低pH值法提取基因组DNA的改进方法 .根据外观、琼脂糖凝胶电泳检测、D2 60nm/D2 80nm比值的测定、限制性酶切反应、PCR扩增的结果表明 ,用改进方法提取的基因组DNA无论在纯度上还是在完整性上都比常规方法要好 .其中改进SDS裂解的高盐低pH值法是提取鹤望兰基因组DNA的最好方法 .图 3表2参 18Strelitzia reginae Banks is rich in polysaccharides, polyphonels, and other secondary metabolites. Therefore, it is difficult to obtain high quality and quantity of genomic DNA from its tissues by conventional methods such as SDS method, CTAB method, and low pH medium with high salts method (LPHS). Modified procedures for DNA extraction from S. reginae are reported based on the above mentioned conventional methods, including the pretreatment of cell separation reagent to collect the nuclei and the use of β mercaptoethanol, polyvinylpyrrolidone (PVP) to inhibit the oxidization, and activated charcoal to clear the secondary metabolites, then followed by the original above mentioned methods to extract the total genomic DNA. The results showed that the modified methods were better than the original ones in terms of purity and totality of the genomic DNA. Among them, the LPHS by SDS cleavage is the best in quality, cost and simpleness of extraction. Fig 3, Tab 2, Ref 18厦门市园林局科技项目 (K990 2 )~

Development of a quantitative ELISA detection method for Coxsackievirus A group 16 strain(CA16) antigen

目的:建立柯萨奇病毒A组16型(CA16)抗原的双抗体夹心ElISA定量检测方法,用于CA16灭活疫苗的研发和生产过程的抗原定量检测。方法:以CA16中和单抗T26H12为包被抗体、nA14b9为标酶抗体,构建定量检测CA16抗原的双抗体夹心ElISA方法,并对方法的特异性、灵敏度、精密度、准确性、线性和稳定性进行分析。结果:建立了双抗体夹心定量检测CA16抗原的ElISA方法。方法的线性相关系数r2=0.998,线性范围为8~128 ng/Ml,定量限度为8 ng/Ml;变异系数CV80%;与CA16以外的其他样本没有交叉反应。结论:构建的CA16抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于CA16疫苗的研发和生产过程的抗原活性的定量检测。Objective:To develop an a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Coxsackievirus A Group 16 Strain(CA16) antigen.This method was used to determine CA16 antigen content at each stage of CA16 vaccine developing and manufacturing process.Methods:A double antibody sandwich Q-ELISA was developed to determine concentration of CA16 antigen,which was based on the high-affinity neutralizing monoclonal antibodies T26H12 as capture antibodies,and NA14B9 as HRP-labeled antibody.The performance of reagent were evaluated.Results:The Q-ELISA for CA16 antigen content was successfully developed.The reagent had good performance.The quantitation scope was 8-128 ng/ml,the coefficient correlation was 0.998,the limit of detection was 8 ng/ml,the recovery was between 87% and 113.8%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and thereagent was no reaction with other sample except CA16 antigen.Conclusion:The Q-ELISA for CA16 antigen was developed with good specificity,accuracy,precision and stability.The method can be used to determine CA16 antigen content during development and production of CA16 vaccine

Development of aquantitative ELISA detection method for Varicella Zoster Virus(VZV) antigen

目的:建立水痘-带状疱疹病毒(VzV)抗原的双抗体夹心ElISA定量检测方法,用于质控VzV灭活疫苗研发和生产中抗原含量。方法:以VzV中和单抗5f6C8为包被抗体、8H5d1为酶标抗体,构建定量检测VzV抗原的双抗体夹心ElISA方法,并对本方法的特异性、灵敏度、准确性、线性和稳定性等性能进行分析。结果:建立的双抗体夹心定量检测VzV抗原的ElISA方法,线性范围为0.4μg~13μg/Ml,相关系数为r2=0.994,定量限度为0.4μg/Ml;变异系数CV80%。与VzV以外的相关病毒样本没有交叉反应。结论:构建的VzV抗原ElISA定量检测方法的各项性能符合定量检测需要,可用于VzV灭活疫苗的研发和生产过程的抗原含量检测。Objective:To develop a quantitative enzyme linked immunosorbent assay(Q-ELISA) to determine the concentration of Varicella Zoster Virus(VZV) antigen.This method was used to determine VZV antigen content at each stage of VZV inactived vaccine developing and manufacturing process.Methods: A double antibody sandwich Q-ELISA was developed to determine concentration of VZV antigen,which was based on the the high-affinity neutralizing monoclonal antibodies 5F6C8 as capture antibodies,and 8H5D1 as HRP-labeled antibody.The performance of reagent were evaluated.Results: The Q-ELISA for VZV antigen content was successfully developed.The reagent had good performance.The quantitation scope was 0.4 μg~13 μg/ml,The coefficient correlation was 0.994,the limit of detection was 0.4 μg /ml,the recovery was between 87.5% and 111.6%.The stability was up to 80% after reagent was heated for 6 days at 37℃.The variation coefficient was lower than 15%,and the reagent was no reaction with other sample except VZV antigen.Conclusion: The Q-ELISA for VZV antigen was developed with good specificity,accuracy and stability.The method can be used to determine VZV antigen content during development and production of VZV inactived vaccine

Application of TWI bus module design in electrical control systems of vehicle bodies

利用AVr单片机的TWI总线构建了汽车车身电控系统的模块化构架。利用TWI总线定义了数据传输协议,建立了基于TWI的通讯信道,实现了数据在主从模块之间的实时通讯。将总线节点尽可能多地贴近车用电器,进而减少车身线束与总线通讯成本。A module framed structure for electrical control systems of vehicle bodies is constructed based on TWI bus of AVR single chip in this paper.The data transmission protocol is defined, TWI-based communication channel is built, and real-time data communication between host module and slaver modules is got.The bus nodes are designed to close to the appliances as nearly as possible, which contributes to reduce the wiring harness and communication costs.厦门市科技计划资助项目(项目编号:3502Z20123013