呼吸道合胞病毒L蛋白单克隆抗体的制备

目的:制备人呼吸道合胞病毒L蛋白单克隆抗体,并对其进行性质鉴定。方法:选取RSV-A2 L蛋白上具有亲水性可能的loop区段,将这些目的片段展示在HBc149上进行原核表达并纯化。重组蛋白免疫6~8周BALB/c雌性小鼠,根据免疫后血清的ELISA和WB分析结果选择L3935和L4099组小鼠进行脾脏免疫。融合后,用间接ELISA法筛选和多次克隆化,获得稳定分泌抗L蛋白单克隆抗体细胞株,并结合ELISA、免疫荧光和免疫印迹对所获得的单抗进行鉴定。结果:获得11株L蛋白特异性单克隆抗体,亚类鉴定结果 IgG1、IgG2a和IgG3单抗各3株,IgG2b单抗2株。结论:成功制备了L蛋白特异性单克隆抗体,也为RSV感染的免疫和发病机制研究奠定基础。国家自然科学基金(No.81401668)项

戊型肝炎病毒核酸阳性血浆经输血传播感染恒河猴的研究

目的 了解戊型肝炎病毒(HEV)核酸阳性血浆对灵长类动物的感染性和致病性。 方法 对抗-HEV IgM阳性而IgG阴性志愿献血员血浆进行HEV RNA检测,并将存在病毒血症献血员的10ml血浆静脉输入健康恒河猴,观察其对恒河猴的感染性和致病性。 结果 从1份抗-HEV IgM阳性而IgG阴性志愿献血员血浆中分离出HEV基因IV型RNA片段。该份血浆输入恒河猴后,恒河猴出现典型急性肝炎生物化学和病理表现,病毒血症,血清抗-HEV IgM和IgG抗体阳转。 结论 HEV病毒血症献血员血浆输入可以引起灵长类动物的HEV感染以及急性肝炎,提示HEV经输血传播的可能性

Simulation research on anaerobic digestion biogas generation from low-grade biomass

低劣生物质厌氧消化可以减少温室气体的排放并且生产生物甲烷作为能源。介绍了关于厌氧消化过程、底物的相关理论,还对目前主要用于厌氧产甲烷过程研究的数学模型以及碳氮磷转化的模拟研究进行了综述。其中,一级动力学模型是最为简单的数学模型,其可以通过简单的计算得到整个过程中甲烷产量随着时间的变化曲线,但是只限于较准确模拟甲烷产率的AdM1模型相对发展最为全面、应用最为广泛,且能够针对具体要研究的对象进行模型的修改。同时总结了较为常见的底物厌氧产甲烷研究模型、研究对象及结果、已有碳/氮/磷转化模拟研究及相关研究,并对开展针对厌氧产甲烷过程中碳氮磷转化的模拟研究进行了展望。Anaerobic digestion of low-grade biomass has attracted increasing interest in reducing greenhouse gas emission and facilitating sustainable development of energy supply.The theory of anaerobic digestion biogas generation and feedstocks are presented in this paper.It provides a review on mathematical model of and simulation research on the conversion of C, N, P in the process of anaerobic digestion.First order kinetic model is the simplest mathematical model which can simulate the dynamics of methane production.The advanced mathematical ADM1 is most popular, and simulates the conversion of C, N, P in anaerobic digestion.The model, simulation subjects and results of anaerobic digestion biogas generation of common substrates are given.Methane yield is the main subject of simulation investigation which is studied in almost all simulation researches on anaerobic digestion biogas generation, and some research reports the variation of volatile solid, volatile fatty acid, COD, CH4, CO2 and inorganic carbonate in the process of anaerobic digestion through mathematical modeling, with which the conversion of C can be determined.Simulation researches on the conversion of N include variations of ammonia nitrogen, inorganic nitrogen and total nitrogen.Simulation research on the conversion of P from sludge digestion is also presented.The challenges and future research trends of the conversion of C, N, P in the process of anaerobic digestion are forecasted.国家重点基础研究发展计划项目(2013CB733505)~

The preparation of ZnSe Qdots in aqueous solution and the effects of photo-induced fluorescence enhancement

在水相中,以巯基乙酸(TgA)为稳定剂制备了具有短波长荧光的znSE量子点.研究了znSE量子点光诱导荧光增敏的机理,并提出通过补加zn2+和TgA以提高光诱导荧光增敏效率以及所得znSE量子点的稳定性这一新思路.研究结果表明,提高补加的zn2+和TgA的量即可增加znSE量子点表面znS壳层的厚度,更好地钝化其表面,从而不仅可显著提高znSE量子点的荧光量子产率(最高可接近15%),而且可大大地提高其表面的抗氧化性和荧光稳定性.ZnSe Qdots with short wavelength-fluorescence were prepared in aqueous solution with thioglycolic acid(TGA) as capping reagent.The mechanism of photo-induced fluorescence enhancement was studied and it was proposed a novel approach involved in the addition of compensatory Zn2+ ions and TGA to the original ZnSe solution to increase the efficiency of photo-induced fluorescence enhancement and stability of ZnSe Qdots.The research indicated that the thickness of ZnS shell increases with the increase of the quantity of Zn2+ ions and TGA.The ZnS shell made a better passivation of the ZnSe Qdot surface,thus resulted in not only higher fluorescence quantum yield(maximal 15%),but also higher stability of ZnSe Qdots.It was expected that the proposed approach would also provide a novel route to increase the fluorescence quantum yield and stability of other types of Qdot prepared in aqueous solution.浙江省自然科学基金资助项目(Y4080518

Determination of pyrimidine and nucleosides using polymer column by HPLC

Using polymer column we established reversed phase high-performance liquid chromatography （ RP-HPLC） method to determine five pyrimidine and nucleosides. The influences of different factors in mobile phase on separation and analysis were studied, and th

Preparation of Intercalated Layered Double Hydroxides and Its Adsorption of Phosphate in Biogas Slurry

通过恒定PH法成功合成了均苯四甲酸插层的锌铝类水滑石(zn2Al-PMA-ldHS),考察了它的特征结构及组成通式,并对沼液中磷的吸附性能进行了研究.结果表明,zn2Al-PMA-ldHS对四川遂宁沼气工程所产沼液中磷的吸附性能要大大优于未插层的锌铝类水滑石(zn2Al-Cl-ldHS).当吸附剂用量为0.1g/l时,zn2Al-PMA-ldHS在4H内,对沼液中磷的吸附量由未插层前的11.58Mg/g提高了约248%,达到40.31Mg/g.同时该吸附剂对沼液中磷酸根的吸附过程符合准二级吸附动力学模型,吸附等温线表现为lAngMuIr型.用0.1 MOl/l nAOH溶液对吸附后的zn2Al-PMAldHS进行解吸再生,经过4个循环周期,其对沼液中磷的吸附量仍能达到首次吸附量的70%左右.ZnAl layered double hydroxides intercalated with pyromellitic acid(Zn2Al-PMA-LDHs)was synthetized at pH of 8.0±0.2to maintain the coprecipitation at low supersaturation.The structure and composition of Zn2Al-PMA-LDHs were investigated,and the general formula was confirmed.Results indicated that Zn2Al-PMA-LDHs had a better adsorbability for phosphate than the controlled Zn2Al-Cl-LDHs in biogas slurry from Biogas Engineering of Suining Sichuang.The phosphate uptake of Zn2Al-PMALDHs was 40.31mg/g when the adsorption dose was 0.1g/L in 4h,however,the phosphate uptake of Zn2Al-Cl-LDHs was only11.58mg/g.The adsorption process followed pseudo-second-order kinetics,and the adsorption isotherms showed a good fit with a Langmuir-type equation.The desorption of phosphates from Zn2Al-PMA-LDHs can be achieved using 0.1 mol/L NaOH solution.And the phosphate uptake of Zn2Al-PMA-LDHs after 4adsorption/desorption cycles was about 70% of that in the first cycle.国家重点基础研究发展计划(973)项目(2013CB733505

Alkaline extraction and acid precipitation of phenolic compounds from longan (Dimocarpus longan L) seeds

National Basic Research Program of China [2013CB733505]; National Natural Science Foundation of China [51378444]; programe for New Century Excellent Talents in University [NCET-13-0501]The alkaline extraction and acid precipitation of longan seed phenolic compounds were investigated to develop a more practical process than currently exists to isolate these chemicals. By using an alkaline buffer as the extraction solvent, the yield of total phenols reached 46.86 +/- 0.43 mg/g after conventional solid-liquid extraction. Because of the benefit achieved from the preconcentration process, an acid precipitation efficiency of 65.29 +/- 0.38% was obtained, and the separation yield of phenols reached 22.04 +/- 1.95 mg/g. However, the purity of the phenolic extract was influenced by the presence in the precipitate of proteins and carbohydrates, which both moved together in all four precipitation fractions at different pH values. The FRAP assay and the assay of the radical-scavenging activity revealed the strong antioxidant activity possessed by the phenolic compounds in the precipitate extracts. This preparation process for phenolic compounds has obvious advantages, such as easy operation, safe products and low production cost, which indicated its prospective industrial potential. (C) 2014 Elsevier Ltd. All rights reserved