497 research outputs found

    Design and Implementation of Intelligent Power Line Fault Diagnosis System Based on GPRS and ARM 7

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    电网的故障检测系统是配电自动化及管理系统的重要组成部分。配电自动化及管 理系统是一套基于统一配电GIS平台下,以配电SCADA系统为配电网实时监控中心, 实现配电网的运行、监控及事故处理的自动化。以配电管理系统为配电网生产、运行、 管理的核心,通过可监视、可定制、可控制的配电管理系统,实现配电工作管理信息 化、数字化、自动化的电网自动化系统。随着电网规模的不断扩大,对电力设备的可 靠性,电力系统的稳定性及安全性提出了更高的要求。在此背景下,需要大力提高对 电网监测的实时性及智能化水平。 本文主要论述了以无线传输GRPR和嵌入式实时操作系统μC/OS-II为核心的智能 电网故障检...Power line fault diagnosis system is an important part of power distribution automation and management system (DAMS) which is based on the uniform GIS platform. As a real-time monitoring center, power distribution SCADA system executes the automation of running, monitoring and accident handling of DAMS. Meanwhile, power distribution management system (PDMS...学位:工程硕士院系专业:软件学院_工程硕士(软件工程)学号:X200923049

    能源行业的竞争格局分析——以电力行业为例

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    文章从国家安全、资源、环境和政府作为的角度将能源的约束条件进行了分类讨论,具体分析了电力行业的竞争现状,并针对这些竞争约束条件提出了四点建议

    Routing Protocol of Wireless Mesh Network Based on Cross-layer

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    无线MESH网络的很多技术特点和优势来自于MESH多跳路由。因此,路由协议的研究与设计是无线MESH网络技术的一个重要课题。由于无线MESH网络有自身负载均衡、路由容错与网络容量等要求,因此运用跨层设计,采用更好的路由参数,使用多径路由等方法已经成为无线MESH网络路由协议设计的重要思路。根据无线MESH的网络特点,按照其路由协议的设计要求,分析了路由协议dSr在MESH网络中的不足,引入跨层设计的方法,提出了采用路由质量路径帧投递率(Pfdr)为路由准则,并具有负载平衡、拥塞避免的路由协议CMrP。分析和仿真结果表明,CMrP在保持MESH网络自身优点的同时,比起dSr在网络性能上有了较大的提升,在吞吐率方面有了很大的提高,相应的时延、抖动参数均有了大幅的下降,更加适用于无线MESH网络。Wireless Mesh Network(WMN) is a technology with outstanding technical characteristics and advantages,many of which originate from the muti-hop routing.Therefore,the study and design of routing protocol is of utmost importance for WMN.In order to satisfy the requirements of WMN such as load balancing,fault tolerance,network capacity,techniques such as cross-layer design and multi-path routing may have to be considered in protocol design for WMN.The deficiencies of Dynamic Source Routing(DSR) protocol applied in WMN is analysed,and an improved routing protocol Cross-layer Multi-path Routing Protocol(CMRP) is proposed,using PFDR(Path Frame Deliver Rate) as the measure.Analysis and simulation results show that CMRP greatly improved the network performance,in load balancing,congestion avoidance,link failure sensing mechanism and the capability of multi-path routing.福建省自然科学基金资助项目(A0710022

    Environment Security Monitoring System for Offshore Caged Fish and Water Quality

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    为了更好地开展渔业养殖中深水网箱中鱼群状态与水质环境的安全监测,提出了一种深水网箱鱼群与水质环境安全远程监测系统.该系统使用声学方式对鱼群分布状况进行监测;同时采用传感器对水质环境的温度、溶解氧、p H值等进行监测,监测结果通过无线数据传输方式实时传送至微处理器,完成信号去噪、数据提取、显示及报警等.实验结果表明,该监测系统性能稳定可靠,结合嵌入式控制技术与无线数据传输技术,监测操作简便,移动性好;同时兼顾鱼群与水质环境安全,监测更全面到位,在推广深水网箱养殖安全配套设施上具有良好的应用前景.Environment monitoring of fish activity state and water quality is the key to offshore cage safety management.A remote monitoring system for offshore caged fish and water quality is developed in the research.The fish distribution and abundance are monitored strategically using acoustic transducers in the system.Meanwhile,some parameters of water quality are obtained using various sensors,such as temperature sensor,dissolved oxygen sensor and p H sensor. Then the collected data are sent to the user monitor terminal in real time through wireless transmission module.Finally,the results are displayed on a 7-inch liquid crystal display screen( LCD) after data processing. The experiment has been conducted to verify the excellent performance of this system.Combined with embedded control technology and wireless data transmission technology,the flexibility and mobility are improved significantly. Besides,both fish activity and water quality are taken into account,making the monitoring more comprehensive.The results suggest that the system has good application prospects.国家海洋局海洋公益性行业科研专项(201505027-2

    社团文化视角下的图书馆文化建设探究——以厦门大学图书馆为例

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    社团文化是校园文化建设的重要部分,图书馆是校园文化建设的中心地带。为了推动社团文化、图书馆文化乃至校园文化的整体发展,本文从社团文化视角下对图书馆文化建设展开研究,阐述了社团文化及图书馆文化的基本内涵,提出了将社团文化融入图书馆文化建设的重要意义和基本思路,并介绍了厦门大学图书馆的实践经验

    人CD4胞外区蛋白在杆状病毒-昆虫细胞系统中的表达纯化及性质鉴定

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    目的:通过条件优化实现人CD4蛋白胞外区在杆状病毒-昆虫细胞表达系统中的高效表达,并对纯化获得的人CD4蛋白胞外区进行抗原性与免疫原性分析。方法:通过选择人CD4分子胞外片段构建重组杆状病毒(p Ac-CD4),然后感染昆虫细胞进行蛋白的表达,采用镍离子亲和层析纯化。运用SDS-PAGE、Western blot、体积排阻色谱、ELISA、SPR法等分析纯化得到目的蛋白的理化性质和抗原性。通过弗氏佐剂与目的蛋白混合免疫BALB/c小鼠,监测小鼠免疫血清的抗体生成。结果:经纯化可获得纯度90%以上的人CD4蛋白,每升细胞培养液最终可获得11.2 mg的目的蛋白,且该人CD4胞外区蛋白具有良好的抗原性。小鼠血清监测结果显示人CD4蛋白能有效刺激机体产生免疫应答,显示其具有良好的免疫原性。结论:本研究通过杆状病毒-昆虫细胞系统进行高效表达,获得抗原性和免疫原性良好的人CD4胞外区蛋白,为HIV受体和感染的研究奠定基础。国家自然科学基金(No.81671645

    Electro-driving Permeation Performance of Nylon 6,6 Membrane

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    电驱动条件下膜分离性能的研究对膜在微流控芯片等微小器件中的应用具有重要的指导意义.研究了界面聚合的尼龙6,6膜的电驱动分离性能,并考察了电场强度、通电时间和温度等操作条件对SO42-和Cl-离子透过性能的影响.结果表明尼龙6,6膜具有较好的SO24-和Cl-离子透过性能,并且透过性能差异不大,离子透过百分比随电场强度和通电时间的增加而增加,而随温度的增加基本保持不变.而对fITC标记的甘氨酸和赖氨酸则能完全截留,截留分子量在500左右,具有部分纳滤膜性质.该膜可望应用于微型器件中氨基酸等有机与生物大分子的电驱动分离和浓缩.The study of separation properties of membrane under electro-driving condition is of an important guiding significance for the application of membrane in microdevices such as microfluidic chip.The electro-driving permeation performance of SO 24- and Cl- of Nylon 6,6 membrane which was prepared by interfacial polymerization was investigated, and effects of electric field, operation time and temperature on the SO 24- and Cl- permeation performance of Nylon 6,6 membrane were studied.Experimental results showed that the permeation percentage of SO 42- and Cl- are high and similar for nylon 6,6 membrane.Ion permeation percentage was increased with electric field strength and operation time, but not changed with temperature.However, FITC labeled glycine and lysine were blocked totally, the molecular weight cutoff is around 500.So in a broader sense, the synthesized nylon 6,6 membrane is one kind of nanofiltration membrane and could be used to separate and concentrate big organic or biological molecules in miniaturized devices.国家自然科学基金(No.20675066);国家基础科学人才培养基金(No.J0630429)资助项

    乙型肝炎病毒体外感染和复制的细胞模型

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    慢性乙型肝炎病毒(Hepatitis B virus,HBV)感染是严重威胁人类生命健康的世界性公共卫生问题。基于现有抗HBV药物的治疗策略,仅能在极少部分患者中实现慢性乙肝的功能性治愈。发展更为有效的抗HBV药物,需要更加透彻全面地认识各个病毒组分和关键宿主因子在HBV感染和复制生命周期中发挥的功能和机制,并在此基础上发现鉴定新的治疗靶点。支持HBV体外感染和复制的细胞模型,是研究HBV生活史的重要工具,并在治疗新靶点的发现和候选药物功效评估等研究工作中发挥关键作用。本文对支持HBV感染和复制细胞模型的新近研究进展进行梳理分析,并对这些模型的应用特点和局限性、新近研究进展和未来发展方向进行系统阐述和讨论。国家自然科学基金(81672023)~

    社会网络环境下用户参与图书馆数字资源组织与整合的需求调查——以部分高校为例

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    设计三套内容基本相同的问卷,就高校图书馆用户、专家、馆员对社会网络环境下用户参与的图书馆数字资源组织与整合的认识、需求和应用,从不同角度开展调查。根据调查数据和发现的问题,提出建议:强化用户宣传教育,倡导体验式技能培训;借助用户力量,加强数字资源组织质量控制;嵌入用户环境,推动数字资源组织与整合的学科化;挖掘用户需求,促进数字资源组织与整合的个性化。国家社科基金项目“社会网络环境下用户参与的图书馆数字信息资源建设模式研究”(项目编号:11BTQ043)成果之

    Establishment of MDCK cell lines which stably express visualable human neonatal Fc receptor

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    [目的]建立稳定表达融合EGFP的人新生儿Fc受体(h FcRn)的MDCK细胞株。[方法]构建重组慢病毒质粒p EGFP-h FcRn,采用四质粒包装系统共转染HEK 293T细胞生产重组慢病毒,感染MDCK细胞后对EGFP阳性细胞进行流式单细胞分选;通过Western Blot及EGFP-β2m荧光共定位验证h FcRn的完整性,并用流式细胞仪检测h FcRn与人Ig G的结合活性。[结果]测序结果表明成功构建p EGFP-FcRn慢病毒表达载体;感染后EGFP阳性MDCK细胞比例约为26.5%,流式单细胞分选后得到纯阳性细胞;荧光共定位及Western Blot均检测到h FcRn的完整表达;流式分析表明细胞株上的h FcRn与Ig G存在p H依赖性结合。[结论]成功获得稳定表达具有生物活性的可视化h FcRn的MDCK细胞株。[ Objective] To establish MDCK cell line stably expressing EGFP- human neonatal Fc receptor(hFcRn) fusion protein. [ Methods ] The lentiviral expression vector for EGFP - hFcRn fusion protein was constructed. Generating by co - transfection of four -plasmids into HEK 293T cells ,the lentivirus particles were used to infect MDCK cell line. EGFP positive single cell was obtained by FACS, and then FcRn expression was identified by fluorescence co -location with EGFP - β2m and confirmed by Western Blot. Flow cytometry was used to detect binding activity of hFcRn and human IgG. [ Results ] DNA se- quencing demonstrated that the lentivirus vector pEGFP - FcRn was constructed successfully. The percentage of EGFP - posi- tive ceils was about 26.5% after infection. Expression of the complete protein was detected through fluorescence co - location and Western Blot, respectively. Flow cytometry analysis showed that the cell lines could pH - dependently capture human IgG. [ Conclusion] MDCK cell line stably expressing functional visualable hFcRn was established.基金项目:国家自然科学基金项目(“结构生物学指导的HBV治疗性抗体人源化及其关键技术研究”,No.31600748;“抗呼吸道合胞病毒高中和活性抗体的保护机制研究”,No.81401668;“基于广谱中和单抗的通用型流感疫苗设计及其结构基础研究”,No.31670934
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