多重荧光PCR同时检测转基因成分35S和Nos方法的建立

根据商品化转基因作物中常用的花椰菜花叶病毒启动子 (CaMV 35S)和根癌农杆菌终止子 (Nos)的序列特点 ,设计并合成了两对引物和相对应的荧光双链探针 ,建立一种应用荧光双链探针的多重荧光PCR同时检测转基因成分 35S启动子和Nos终止子的方法 .并利用该方法对马铃薯、大豆、玉米、甜椒、番茄等 11份实物样品进行了检测 ,其中有 5份样品结果阳性 .结果表明所建立的多重荧光PCR方法能同时检测出 35S和Nos双组分 ,较常规PCR技术更为简便、快速、准确 ,有很好的应用前景

黄连素对精神分裂症患者血脂水平影响的Meta分析

目的系统评价黄连素对精神分裂症患者血脂水平的影响,为精神分裂症患者血脂干预方案的选择提供参考。方法系统检索英文数据库(PubMed、PsycINFO、Embase、Cochrane Library)和中文数据库(中国期刊全..

动力学对称性研究的拓扑途径——互作用玻色子模型中的应用示例

运用拓扑观点对IBM系统动力学对称性破坏的过程进行了研究,着重揭示了与能级免交叉联系的奇异行为及其根源。它实际上是动力学映射的不稳定不动点所导致的结果。越过不稳定不动点后,为了给出连续映射,就必须涉及能级的置换操作,因而呈现出能级免交叉的现象,并使映射不再能表示为动力学群生成元的函数,于是动力学对称性局域地实质性地受到了破坏

新疆塔里木盆地北部碳酸盐裂缝性储层测井资料综合评价方法研究

该成果为在“七五”攻关基础上所研制的测井综合评价方法和计算机处理系统。通过岩芯分析结果与测井资料的相关对比，特别是通过综合地质录井信息得到的综合裂缝概率与岩芯分析的裂缝孔隙度的相关对比，建立了利用测井资料裂缝发育程度和裂缝分级的方法，效果较好，有明显的实用价值。采用改进的有限元法进行了裂缝模型的正演模拟，为研究裂缝带及其分级提供了理论根据。建立了包含原“七五”的解释模型（ＣＦＩＳ）的碳酸盐岩裂缝性储层综合评价系统（ＦＲＡＣ）和相应程序。该系统具有岩性判别与测井解释参数选择、裂缝带识别、储层参数估算和油水性质判别等功能；综合了包括声波全波列在内的多种测井参数，拾取多种裂缝指标，并把地质录井信息综合到神经网络技术之中，将如此众多的参数和先进解释技术同时用于碳酸盐岩裂缝性储层测井评价，取得了明显效果

An Investigation of Stress and Mood State of Employees in State-Owned Enterprise

目的 :研究国有企业职工的压力及心境状态特征。方法 :采用自编的社会应激事件调查量表和简明心境状态量表 ,抽取某国有企业集团 4 74 5名职工进行问卷调查。结果 :不同群体的国有企业职工压力和心境状态得分不同。管理人员组的压力总分显著高于一般职工组和下岗职工组。压力总分与心境状态得分之间呈现显著正相关。结论 :国有企业职工的压力水平和心境状态在不同群体中存在差异 ,承受的压力对心境状态有一定的影

国有企业职工的压力与心境状态调查

目的 :研究国有企业职工的压力及心境状态特征。方法 :采用自编的社会应激事件调查量表和简明心境状态量表 ,抽取某国有企业集团 4 74 5名职工进行问卷调查。结果 :不同群体的国有企业职工压力和心境状态得分不同。管理人员组的压力总分显著高于一般职工组和下岗职工组。压力总分与心境状态得分之间呈现显著正相关。结论 :国有企业职工的压力水平和心境状态在不同群体中存在差异 ,承受的压力对心境状态有一定的影

Population pharmacokinetic modeling of methylphenidate prolonged-release tablets clearance in patients with attention deficit/hyperactivity disorder by nonlinear mixed effects modeling

目的建立注意缺陷多动障碍患者(AdHd)哌甲酯缓释片清除率的群体药动学模型。方法前瞻性收集闽南地区服用哌甲酯缓释片治疗的78例AdHd患者的消除相血药浓度(n=165),采用非线性混合效应模型(nOnMEM)法分析数据。结果最终模型为Cl(l·H-1)=254·(WT/34.7)0.663·0.884(如果合用丙戌酸钠,否则为1)·EXP(ETA(Cl)),WT为体质量(kg)。结论成功建立AdHd患者哌甲酯缓释片治疗的群体药动学模型,根据患者的生理用药资料,可估算其清除率,为制定给药方案提供依据。AIM To establish a clearance model for methylphenidate prolonged-release tablets(MPH)in patients with attention deficit/hyperactivity disorder(ADHD)by population pharmacokinetic method.METHODS Serum concentration data(n=165)in elimination phase were collected prospectively from 78 ADHD patients in south Fujian during their routine clinical treatment.Nonlinear mixed effects modeling(NONMEM)was employed to establish the population pharmacokinetic model of the MPH clearance(CL).RESULTS The final model was:CL(L·h-1)=254·(WT/34.7)0.663·0.884(if taking sodium valproate,otherwise it equaled to 1)·EXP(ETA(CL))in which WT was weight(kg).CONCLUSION A population pharmacokinetic model is established successfully and proposed to estimate the individual CL for patients taking MPH in terms of patients' characteristics and dosing history,and to establish a prior dosage regimen.福建省卫生厅青年科研课题(编号2013-2-02); 厦门市卫生局科研立项(编号3502Z20077079

Probe melting curve analysis for rapid detection of injectable second-line drug-resistant mutations in Mycobacterium tuberculosis strains

目的评价结核分枝杆菌注射类二线药耐药突变检测试剂盒MElTPrO Tb/Sl的分析性能以及临床性能。方法首先使用企业参考品对试剂盒的突变检出能力进行考察,之后将野生型基因组dnA以10倍梯度稀释后考察试剂盒的最低检测限,使用野生型质粒以及含常见耐药突变的质粒以不同突变比例混合考察试剂盒检测不均一耐药样本的能力,并使用23株非结核分枝杆菌标准株考察试剂盒的分析特异性,最后使用241份临床分离株对试剂盒进行临床评价。结果该试剂盒可以将9种耐药相关突变与1种野生型多态性与野生型进行区分。可以检测到低至5个拷贝的野生型基因组dnA。当突变比例为30%或更高时,试剂盒可以将其与野生型进行区分。23种非结核分枝杆菌的结果显示该试剂盒有良好的特异性。和比例法药敏试验进行对比,该试剂盒的临床灵敏度为卡那霉素90.0%(9/10),阿米卡星80.0%(8/10),卷曲霉素85.7%(6/7);特异性为卡那霉素98.8%(85/86),阿米卡星98.8%(85/86),卷曲霉素96.6%(86/89)。试剂盒检测结果与测序结果一致。结论该试剂盒灵敏度高、特异性好,可以快速、准确地检测结核分枝杆菌注射类二线药常见的耐药突变,有望应用于临床。The MeltPro TB/SL assay was first evaluated by a reference panel containing 10 plasmids of different mutations to evaluate the analytical and clinical performances.The limit of detection(LOD)was studied by purified genomic DNA of a reference strain H37 Rv that was serially diluted in ten-fold.The wild-type and mutant plasmids of different mutation types were mixed with serial percentages of mutation to evaluate the detection ability of mixed bacterial samples of this kit.The analytical specificity was evaluated using 23non-tuberculous mycobacterium(NTM)isolates.The clinical performance was evaluated by analyzing 241 clinical strains.Ten plasmids of different mutations could be clearly identified from the wild-type plasmid.The LOD was 5copies of wild-type Mycobacterium tuberculosis(MTB)genomic DNA per reaction.The assay could detect30% mutant DNA in wild-type background.All the 23 NTM strains showed no cross reactivity with M.tuberculosis strain.Clinical study showed that the clinical sensitivity of the assay was 90.0%for kanamycin,80.0%for amikacin,85.7%for capreomycin,and the clinical specificity was 98.8%for kanamycin,98.8%for amikacin,96.6%for capreomycin,respectively.The MeltPro TB/SL assay gave a 100% concordance with sequencing analysis.The MeltPro TB/SL assay was able to reliably detect common mutations associated with injectable second-line drug-resistance.The assay has the potential to be used for rapid screening of injectable second-line drug-resistant mutations in MTB strains.国家“十一五”重大专项(No.2008ZX10003-004)资助~

Electrochemical Performance of Al-Doped Spinel LiMn2O4 Cathode Material for Li-ion Batteries

尖晶石LiMn2O4作为锂离子电池正极可大电流放电，且成本低、环境友好. 采用溶胶-凝胶法制备尖晶石LiMn2O4及Al掺杂材料. 使用X-射线衍射（XRD）和扫描电子显微镜（SEM）观察材料结构与形貌. 结果表明，复合材料颗粒尺寸300 ~ 500 nm，呈类球形. 电化学恒流充放电测试表明，Al掺杂尖晶石LiMn2O4电极的循环性明显提高，Al掺杂5%LiMn2O4（by mass，下同）正极在1C倍率充放电100周期循环后的容量保持率为98.2%，1C倍率充电、5C倍率放电下，100周期循环后其容量保持率为99.0%，表现出较优的电化学循环性能.Spinel LiMn2O4, a cathode material for Li-ion batteries, is featured as good high-rate performance, low cost and environmental friendly. The pristine and Al-doped LiMn2O4 samples were prepared by sol-gel method. The structure and micromorphology of the as-prepared samples were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The results demonstrated that the as-prepared LiMn2O4 samples had a typical cubic spinel structure and were composed of sphere-like particles with a diameter range of 300 ~ 500 nm. Galvanostatic charge-discharge tests showed that the LiMn2O4 samples doped with Al element exhibited an enhanced cycling performance. Especially, in the case of LiMn2O4 with 5% Al-doped, the discharge capacity retention was about 98.2% at the rate of 1C discharge and was 99% at the rate of 5C discharge, based on the same charge rate of 1C.国家自然科学基金项目（No. 21273119）资助作者联系地址：南开大学新能源材料化学研究所，天津 300071Author's Address: Institute of New Energy Material Chemistry, Nankai University, Tianjin 300071, China通讯作者E-mail：[email protected]