“僵尸企业”问题研究

关于\"僵尸企业\"的识别尚未有统一权威的方法,既有学术标准,也有国内定义。总体来看,\"僵尸企业\"的普遍特征是企业生产经营持续不景气、企业融资风险高、企业外部依赖性强。许多理论模型从银行的微观行为解释了\"僵尸企业\"的形成原因。本文从宏观层面,首先对\"僵尸企业\"的定义进行了界定,并基于\"僵尸企业\"的普遍特征,测算了我国上市公司的\"僵尸企业\"规模情况。结果显示,采矿业、交通运输业、农林牧渔业、批发零售业、制造业、住宿餐饮业等传统行业的\"僵尸企业\"占比较高。从省份来看,一些资源型省份和中西部省份的\"僵尸企业\"占比较高,均高于10%。最后,本文通过研究日本处置\"僵尸企业\"的经验,提出有关我国处置\"僵尸企业\"的政策建议。国家自然科学基金青年项目(项目批准号:71703165)的资

Cloning,expression and identification of aspartic protease gene of Ancylostoma caninum in E.coli

目的对犬钩虫(Ancylostomacaninum)天冬氨酸蛋白酶Asp基因进行了克隆,鉴定,并在原核系统中进行表达。方法以犬钩虫总RNA为模板,用RT-PCR法对犬钩虫天冬氨酸蛋白酶Asp基因进行扩增,获得的AspcDNA产物克隆进pMD-18T载体,用PCR筛选阳性克隆。碱裂解法进行质粒提取,单、双酶切进行鉴定并测序。将测序正确的阳性克隆进行双酶切,构建到表达载体pET-32a中,将此重组质粒先转化到E.coliDH5a内,提取质粒,酶切鉴定阳性,再转化入表达宿主E.coliBL21(DE3)菌株内,对转化菌株用IPTG进行诱导培养。收集培养液,破菌、离心,上清进行SDS-PAGE,通过电转移将胶中蛋白转到硝酸纤维素膜上后进行免疫印迹分析。结果电泳发现转化了重组质粒的菌株有表达蛋白,所表达蛋白相对分子量为40kDa,抗体检测有特异条带大小为40kDa。结论成功进行了犬钩虫天冬氨酸蛋白酶Asp基因的克隆表达。Cloning,expressing and identify the aspartic protease gene from Ancylostoma caninum for the further studying in the application in the diagnosis of Ancylostoma as well as for the development of anti-hookworm vaccine.The gene encoding aspartic protease was amplified from the total RNA by using RT-PCR technique.The amplified product was cloned initially into pMD-18T vector.After PCR selection,enzyme digestion,and sequencing.The positive and right sequence of plasmid was digested by enzyme and ligated with digested expression vector pET-32a by ligase,and then transformed the construct into the competent E.coli DH5а strain.Colonies containing the insert plasmid were selected on LB plus ampicillin(100μg/ml) plate and also by PCR screening,the positive plasmid DNA was extracted and digested with enzymes.Plasmid containing the right insert were sequenced to confirm its identity,and then retransformed the recombinant plasmids into E.coli BL21(DE3)strain.Bacterial lysates from cultures induced with IPTG(1mmol/L) were directly loaded onto SDS-PAGE,and the proteins on the SDS-PAGE gel.Were transferred to nitrocellulose membrane,detected with antiserum against recombinant expressing aspartic protease.Through these procedures,a specific protein with a molecular mass of 40kDa could be visualized on gel.This recombinant expressing protein can be used for further studies in the detection of the effectiveness of immunity and the preparation of antigen and antibodies of aspartic protease in large scale.国家自然科学基金(No30470881);; 福建省自然科学基金重点项目(NoC0320001)联合资

Study on Ancylostoma caninum in dogs and the observation of different stages of life cycle

目的犬钩虫(Ancylostoma canium)幼虫可以感染人体,但不能发育为成虫,仅引起皮肤幼虫移行症。方法采用饱和盐水漂浮法和涂片法检查钩虫卵;用"T"滤纸条培养犬钩蚴;皮肤感染和灌喂人工感染实验狗。感染后不同时间解剖狗,从狗小肠获取钩虫,镜下观察和拍照。结果厦门市30只宠物犬样本犬钩虫感染率为3.33%(1/30),蛔虫感染率为10.00%(3/30)。对犬钩虫生活史各期的形态进行观察。结论厦门市存在宠物犬犬钩虫感染,特别是农家狗的犬钩虫感染为多。Only the larvae of Ancylostoma canium infect human and emigrant in human body,they could not develop into abult.In order to get Ancylostoma canium,we investigated dog hookworm in summer and autumn of 2004.The eggs were floated by saturated NaCl solution and incubated on the "T" filter paper.The experimental dogs were infected with infective larva from skin and mouth.The parasite was collected from small intestine at different time of post-infection and observed via microscope.The results indicate that the infection rate of Ancylostoma caninum is 3.33%,and Ascaris lumbricoides is 10.00% in dogs,respectively.And the observation of different stages of hookworm's lifecycle were done.In conclusion,Ancylostoma canium is still popular in dogs in Xiamen,especially in the countryside.福建省自然科学基金重点项目资助(项目号:C0320001

灾难与旅游

旅游是一个新兴的、具有巨大发展潜力的产业,同时也是一个脆弱的产业。"5.12汶川大地震"对四川旅游所造成的重创,不仅表现以灾难期间,在一个相当长的时间内都将受到严重影响。本文以记实的方式反映四川旅游资源、设施以及最能体现农业旅游特色,也被认为是缘发地的成都"农家乐"在灾难中的情况,试图引起政府、学界以及旅游行业对旅游"警示机制"的建立给予充分的重视

Analysis of the rDNA-sequences from internal transcribed space-3 through RFLP

目的爪哇根结线虫(Meloidogynejavanica)、鼠蛲虫(Enterobiussp·)和犬钩虫(Ancylostomacaninum)是来自不同种属的3种线虫,利用rDNA-ITS2-RFLP方法分析3种不同线虫种间多态性,为将来进一步发展以rDNA-ITS-RFLP分析为基础的土源性线虫的分子诊断方法建立基础。方法应用种间具有较为保守性的遗传区域-内转录间隔区,包括部分的5·8S序列、ITS2和28S的序列,以爪哇根结线虫、鼠蛲虫、犬钩虫3种不同种类线虫的基因组DNA为模板,设计特定引物用PCR扩增rDNA-ITS2+片段,用限制性内切酶HindⅢ、EcoRⅠ、EcoRⅤ、PstⅠ、KpnⅠ和SalⅠ酶切PCR扩增产物进行RFLP分析。结果PCR扩增爪哇根结线虫、鼠蛲虫、犬钩虫得到的ITS2+片段大小分别为1000bp、1800bp、1800bp,用6种限制性内切酶酶切PCR扩增产物,共产生12条酶切片段。用限制性内切酶HindⅢ/EcoRⅠ,PstⅠ/EcoRⅠ,HindⅢ/PstⅠ双酶切爪哇根结线虫、鼠蛲虫和犬钩虫的rDNA-ITS2+的PCR扩增产物,除爪哇根结线虫外,鼠蛲虫和犬钩虫均可得到2条以上的RFLP片段。结论爪哇根结线虫、鼠蛲虫、犬钩虫rDNA-ITS-RFLP分析显示这3种不同线虫rDNA-ITS2+存在着种间多样性,其中爪哇根结线虫为植物寄生性线虫,而鼠蛲虫和犬钩虫均为动物寄生性线虫,说明rDNA-ITS2+-RFLP方法可用来作为判断线虫种间多样性以及诊断和区分不同线虫的有用依据。The polymorphisms of three species of nematodes from different sources, Moloidogyne javanica, Enterobius species and Ancylostoma caninum,were analyzed by means of RFLP in order to establish the basis for the further development of molecular diagnosis of nematodes from soil. In the present study,we used the relatively conserved region,the internal transcribed spacer(ITS) including partial sequnces of 5.8S, ITS2 and 28S and the genome DNA of these 3 different species of nematode as template to design the specific primers for the amplification of the ITS2 sequence. Six restrictive endonucleases,HindIII, EcoRI, EcoRV, PstI, KpnI and SalI, were used for the digestion of PCR products of ITS2. The experimental results showed that the sizes of the PCR products of these 3 species of nematodes were 1000, 1800, 1800 bps respectively. Twelve fragments were obtained after digestion of ITS2 with these restrictive enzymes in which two pairs of them, HindIII/EcoRI, PstI/EcoRI and HindIII/PstI respectively digested the rDNA-ITS2-PCR products,except for Meloidogyne javanica. It is evident that this method of rDNA-ITS2-RFLP is useful for the identification of different species of nematodes.国家自然科学基金(No.30470881);; 福建省自然科学基金重点项目(No.C0320001)资

Method for generation of painting-like artistic effect

图像的绘画效果由平滑纹理,保持并加强边角获得。给出一种鲁棒的由真实图像自动生成相应绘画效果的算法。算法根据图像的局部结构,自适应地调节滤波器的带宽与形状,来模拟画笔宽度和绘画技法。实验结果表明,算法的处理结果在视觉效果上接近绘画,并且对低质量的输入图像同样有效。Painting-like artistic effects can be achieved from photographic images by filters that smooth out texture details,while preserving or enhancing edges and corners.A novel image filter is proposed in this paper to generate painting-like effects.By adaptively changing its bandwidth and shape to image features,this filter can simulate the size of brush and painting skills.A large variety of experimental results show that the output of the proposed operator is visually similar to a painting.The proposed operator is robust to degradations of the input image such as blurring and noise contamination.国家重点基础研究发展规划(973)No.2007CB311005;福建省自然科学基金No.A0710020---

Development of a New Temperature and Humidity Sensitive Integrated Sensor

研制成由硅温敏二极管和聚酸亚胺湿敏电容集成的温湿度传感器。介绍了它的工作原理、结构设计、制作工艺、测试结果以及为提高它的性能所作的一些研究。An integrated temperature and humidity sensor consisting of the silicon diode and the polyimide-based capacitive humidity sensor has been deve-loped.The sensor working principle, structure design, manufacturing technology, measurement results and some studies of improving the sensor characteristics have been introduced.福建省科技资

含氯消毒剂杀灭船舶压舱水中3种优势赤潮藻种的实验研究

〔目的〕研究含氯消毒剂在实验室水平对船舶压舱水中赤潮藻的杀灭效果,为现场实验提供理论依据。〔方法〕通过测定叶绿素a的含量,比较药物作用前后的藻细胞数量,反映药物对试验藻种的量效和时效关系。〔结果〕药物浓度、作用时间与杀灭效果呈现正相关趋势,但较高的作用浓度决定了高昂的投药成本和潜在的环境代价。〔结论〕含氯消毒剂不宜单独用于杀灭压舱水中的赤潮藻类,可以考虑作为压舱水综合处理体系中的高层次处理方法

海洋浮游植物自动分析和识别技术

浮游植物是海洋生态系统的初级生产者,对其种类的分类鉴定和定量分析是海洋科学研究和应用的基础性工作.传统的分析方法主要采用显微镜下的人工鉴定和定量计数.为了适应海洋科学和环境监测中的快速检测的需要,解决目前显微镜人工鉴定中存在的专业水平要求高、分类人员断层、耗时等问题,至今已有不少学者对各种浮游植物自动分析和识别方法进行了研究,包括基于藻细胞形态的与计算机技术相结合的图像法,基于藻类色素组成的吸光光度法、荧光分光光度法和高压液相色谱法,基于藻细胞大小、色素组成、DNA等的流式细胞仪法,基于细胞基因系列的分子探针法等.本文对这些方法的原理、发展动态、优缺点等进行了综述.认为基于藻细胞形态的浮游植物显微图像自动识别技术,由于其综合了现代仪器自动分析和传统显微分类方法的优点,是浮游植物自动识别的一种理想和实用的方法,将有很好的应用前景

抗肺癌单克隆抗体在裸鼠移植瘤中的放射免疫显像

抗肺癌单克隆抗体在裸鼠移植瘤中的放射免疫显像俞浩，瞿跃进，颜江华，盛荣宗，林月华俞丹，苏新辉，毛利娜，苏金华，杨善民我们用131I标记抗肺癌单克隆抗体（MCAb3d3）在移植人肺腺癌裸鼠中进行显像，并研究裸鼠体内生物学分布，现将结果报道如下.材料和方..