肺心病血小板活化与脑梗死的关系研究

目的　研究肺心病血小板活化与脑梗死的关系。方法　应用单克隆抗体法并在流式细胞仪上对血小板活化因子CD62p、P1 0 进行检测 ,同时跟踪检测脑CT ,以了解它们之间关系。结果　肺心病病人血小板异常活化 ,与对照组和未治疗组比较有显著差异 (t >2 69　P <0 0 1)。血小板活化脑梗死机率增加 ,经综合治疗后梗塞灶缩小或消失。结论　肺心病血小板异常活化能加重肺心病和脑梗死病情 ,应用抑制血小板活化药物和适当抗凝可能有利于肺心病脑梗死疗效的提

The effect of low glucose and high lactic acid on the survival of Hela

目的:探讨低糖高乳酸环境对宫颈癌细胞生存的影响,以及对EGFR-mTOR通路的调节作用。方法:将Hela细胞培养于常糖(葡萄糖10mmol/L); 、低糖(葡萄糖3mmol/L)、高乳酸(葡萄糖10mmol/L,乳酸2.5mmol/L)、低糖高乳酸(葡萄糖3mmol/L,乳酸2.5mmol/; L)4种环境下,CCK-8法测定Hela细胞的生长抑制率,流式细胞术测定细胞周期。荧光实时定量PCR法检测EGFR和mTOR; mRNA水平表达。结果:与常糖组相比,高乳酸组的细胞抑制率显著升高(P<0.01),48h细胞G_1/G_0期比例显著升高(P<0.01),细胞; 凋亡率、EGFR和mTOR; mRNA表达水平均无变化。与常糖组相比,低糖组的细胞抑制率显著升高(P<0.01),48h细胞凋亡率显著降低(P<0.01),细胞各周期比例变化; 与常糖组无差异,EGFR表达水平降低(P<0.05)。与常糖组相比,低糖高乳酸组的细胞抑制率显著升高(P<0.01),但低于低糖组(P<0.01; )和高乳酸组(P<0.05),48h G_1/G_0期比例显著升高(P<0.01),细胞诱导凋亡率显著升高(P<0.01),EGFR和mTOR; mRNA表达水平均显著升高(P<0.01)。结论:Hela细胞在低糖高乳酸环境中的存活状况好于单纯低糖和单纯高乳酸环境,且伴随着EGFR和mTO; R基因表达水平上升。Objective:To explore the effect of the low glucose and high lactic acid; environment of survival of cervical carcinoma cells,and the regulation; of EGFR-mTOR signal way.Methods:Hela cells were cultured in four; conditions:normal glucose (glucose 10mmol/L),low glucose (glucose; 3mmol/L),high lactic acid (lactic acid 2.5mmol/L) and low glucose add; high lactic acid (glucose 3mmol/L,lactic acid 2.5mmol/L).Growth; inhibition rate of Hela cell was determined by CCK-8.Flow cytometry; (FCM)were performed to evaluate the cell cycle.The expression levels of; EGFR and mTOR mRNA were detected using real-time quantitative polymerase; chain reaction (real-time PCR).Results:Compared with those in regular; sugar environment,the cell growth inhibition rates were significantly; increased in high lactic acid environment(P<0.01).At 48h,the rate of; G_1/G_0 was significantly increased (P<0.01),while the apoptosis rate of; the cells and the expressions of EGFR and mTOR mRNA had no; change.Compared with those in regular sugar environment,the cell growth; inhibition rates were significantly increased in high lactic acid; environment,(P<0.01),while lower than those in low glucose; environment(P<0.01) and in high acid environment(P<0.05).The rates of; G_1,G_2 and S phase had no change.The expressions of EGFR mRNA was; reduced (P<0.05).The cell growth inhibition rates were significantly; increased in high lactic acid add low glucose environment (P<0.01).At; 48h,the apoptosis rate and the rate of G_1/G_0 were significantly; increased (P<0.01),while the EGFR and mTOR expression levels were also; increased (P<0.05).Conclusion:Hela in the low glucose add high lactic; acid survives better than those in low glucose and in high lactic acid; environment,while the expression of EGFR and mTOR is increased.泉州市科技计划立项项目; 国家面上项

低糖高乳酸环境下吉非替尼诱导HeLa细胞EGFR-TKI耐药的研究

目的探讨低糖高乳酸微环境对表皮生长因子酪氨酸激酶抑制剂(EGFR-TKI)抑制HeLa细胞的影响和可能机制。方法HeLa细胞在常糖(葡萄糖10; mmol/L)和低糖高乳酸(葡萄糖3 mmol/L +乳酸2.5 mmol/L)环境下培养,并分别给予2.67; mumol/L吉非替尼干预。采用CCK-8法测定HeLa细胞生长抑制率,流式细胞术检测细胞周期,荧光定量RT-PCR检测EGFR和mTOR; mRNA水平的表达。结果与常糖组比较,低糖乳酸组24 h和72 h细胞抑制率均显著升高(P < 0.01),48; h细胞抑制率略高于常糖组。与吉非替尼阴性组比较,常糖+吉非替尼组和低糖乳酸+吉非替尼组在24、48、72 h三个时点细胞抑制率均显著上升(P <; 0.01)。与常糖组相比,低糖乳酸组48 h细胞诱导凋亡率显著上升(P <; 0.01),低糖乳酸+吉非替尼组细胞诱导凋亡率较低糖乳酸组显著降低(P < 0.01)。与常糖组比较,低糖乳酸组存活细胞的EGFR和mTOR; mRNA表达水平上调(P < 0.05)。常糖+吉非替尼组的EGFR和mTOR mRNA水平均上调(P <; 0.05)。与低糖乳酸组比较,低糖乳酸+吉非替尼组的EGFR和mTOR mRNA上调水平有显著差异(P <; 0.01)。结论高乳酸低糖环境下吉非替尼可大幅度上调存活HeLa细胞EGFR和mTOR表达水平,可能是诱导HeLa细胞抵抗EGFR-TKI的机制; 。泉州市第一医院青年科研; 泉州市科技计划项目; 国家面上项

Research Progress of Nitrogen Leaching in Farmland Soil

对农田土壤氮素淋失的相关研究进行综述和总结是研究农业面源氮素污染的一个至关重要的前提。本文从土壤氮素渗漏淋失形态和迁移机理、氮素渗漏淋失的研究方法和影响土壤氮素渗漏淋失的因素三方面进行了详细的分析介绍,概述了近年来国内外农田氮素渗漏淋失的研究进展和研究成果,并在此基础上展望了未来氮素淋失研究工作的重点。研究结论对农田土壤的水肥管理措施及浅层地下水硝氮污染的减缓有一定的理论和借鉴意义Reviewing and summarizing the related research of farmland soil nitrogen leaching loss is a crucial basis of studying agricultural non-point source of nitrogen pollution.This paper introduced and analyzed the leaching form and migration mechanism of soil nitrogen,research methods and influencing factors of nitrogen leaching in farmland soil.The recent research progress of nitrogen leaching in farmland soil at home and abroad was summarized,and the future research priorities were pointed out.The research conclusions have some reference values on farmland soil fertilization management measures and slowing down the nitrate nitrogen pollution in shallow groundwater.福建省自然科学基金(2013J01211;2013J01210); 国家自然基金项目(51378446

Testing GLEAMS for Nitrate Leaching Modeling in an Agricultural Catchment of Southeast China

利用我国东南亚热带地区农业小流域不同土地利用方式的硝氮渗漏淋失实测数据检验了GLEAMS(Groundwater Loading Effects of Agricultural Management Systems)模型在该地区的适用性。通过现场试验和实地调查并结合模型手册,获取模型所需的水文和营养盐参数,参考模型参数的敏感性分析结果对模型进行调试。结果表明模型对水稻田除外的其它土地利用方式的硝氮渗漏淋失模拟效果较好。水稻田渗漏模拟效果差的主要原因在于模型的水分平衡方程不能反应水稻田长期淹水的实际情况。模型模拟结果的精度可以接受,从而验证了GLEAMS模型在该流域的适用性。GLEAMS(Groundwater Loading Effects of Agricultural Management Systems)model was introduced to test and validate nitrate leaching in an agricultural catchment in southeast China.Field experiments were performed under different landuses such as paddy,bananas and vegetables,in Wuchuan catchment of Fujian Province with the area of 9.6 km2.Eight boreholes were drilled and monitoring pipes were installed for continuous monitoring of nitrate leaching from April to December in 2002.The hydrologic and nutrient parameters of GLEAMS model were extracted based on measurements and experiments during the main crop growing season.Model parameters were tuned to achieve desired agreements between measurements and model simulations based on sensitivity analysis.After calibration and validation,the model generally had acceptable performances in simulating nitrate leaching throughout the landuses with an exception in paddy field.The model simulations can be used to specifically establish best management practices for nutrient management and pollution mitigation in the catchment.国家自然科学基金(40671116;40301045

Realization of a Monolithic Optoelectronic Integrated Receiver Chip for Optical Communication Transceiver

针对应用于850nM光通信中的10/100MbIT/S收发器,提出采用0.5μM标准CMOS工艺对其光接收芯片实现SI基单片集成。整体芯片面积为0.6MM2,共集成了一个双光电二极管的(dPd)光电探测器和一个跨阻前置放大电路,功耗为100MW,并给出了具体的测试性能结果。结果表明,在850nM光照下,光接收芯片带宽达到53MHz,工作速率为72MbIT/S。重点介绍了dPd光电探测器的原理和结构,并给出了相应的制造过程和电路等效模型,对整个光接收芯片进行了多种实用性测试,可以满足系统的性能要求。Monolithically integrated optical receiver was designed in 0.5 μm standard CMOS technology,used in 850 nm optical transceivers of 10/100 Mbit/s.The chip size is 0.6 mm2,integrating a double photodiode(DPD) detector and a transimpedance preamplifier.The power dissipation is 100 mW,and the result of test about OEIC was given.Test results show that the optical receiver has a bandwidth of 53 MHz and the bit rate of 72 Mbit/s in 850 nm light.The principle and structure of the DPD was introduced,the corresponding manufacturing process and circuit equivalent model were given.The OEIC meets the system performance requirements.厦门市科技计划项目(3502Z20063002

Denitrification Determination in soil of Wu Chuan Agricultural Catchment and the Control Measure

反硝化作用是土壤氮素转化的一个重要过程,为探明五川流域内的农业土壤的反硝化作用强度及其影响因素,利用乙炔抑制-原状土柱培养法对其进行测定。通过3次试验测定,发现五川流域农业土壤具有较强的反硝化作用强度,在种植季节,土壤平均反硝化作用强度为0.1kgN·hm-2·d-1,最高达到0.6kgN·hm-2·d-1,其中蔬菜地反硝化作用强于其他土地利用类型。反硝化作用同土壤的NO3-含量、含水量、温度以及pH都存在正相关关系,它们是流域土壤反硝化作用的主要影响因子。五川流域农业土壤经由反硝化作用氮损失量占流域平均施肥量的16%,高于国内其他地区。针对五川流域的环境和农业经济特点,提出了控制反硝化作用的措施:在温度较低的夜间进行施肥灌溉宜以防止氮肥损失,用农村富余的厩肥代替化肥以减轻反硝化作用的发生,同时加大节水灌溉力度。Denitrification in soil is the main pathway of gaseous nitrogen loss in the catchments.In order to find out the denitrification flux in top-soil and the affecting factors in Wu Chuan catchment,the denitrification rate of top-soil was determined using the acetylene inhibition-intact soil core technique for 5 months.Three times of the denitrification experiment were carried out from Dec.15th 2005 to Apr.18th 2006,at the same time,the chemical and physical properties of the soils were also measured during every experiment.The soils in Wu Chuan catchment belong to typical Haplic red soil,with the chemical and physical properties suitable for denitrification and the organic matter,total N,NO3-N and pH of the soils being 9～25 g·kg-1,0.4%～7.9%,1.5～6.8 mg·kg-1 and 4.9～5.7,respectively.The results indicated that denitrification of the soil showed temporal and spatial variations.The denitrification rate in uplands of the catchment was higher than that in any other places,and was enhanced when there were many rains and when the soil temperature was high.The flux of denitrification was mostly related with fertilization,soil moisture,air temperature and pH.Air temperature affected denitrification significantly.The gaseous N loss rate through denitrification represented about 16% of the fertilizer applied in the catchment scale.Also,the denitrification flux of the top-soil in Wu Chuan catchment was much higher than that in other places of China,and the denitrification rate of soils in south was higher than that in north.The soils in Wu Chuan catchment may be the source of atmospheric nitrous oxide gas.Since the high level of agricultural economic development in Wu Chuan catchment and the frequently used fertilizers,the flux of fertilizers is much higher than other agricultural region in China.Based on the environmental characteristics and agricultural economy in Wu Chuan catchment,the following control measures should be adopted:(1) fertilization and irrigation in the low-temperature night to prevent fertilizer loss;(2) utilization of livestock waste instead of fertilizer to inhibit denitrification;(3) popularizing economical irrigation to avoid reductive situation in the soil.国家自然科学基金(40301045和40671116

Evaluation of chemiluminescence immunoassays kits for detection of influenza A virus

目的考核甲型流感化学发光检测试剂的灵敏度和特异性。方法分别利用病毒分离培养液和入境人群的鼻咽拭子标本考查甲型流感试剂盒的检测灵敏度和特异性。结果化学发光法对H1、H3、H5、H7、H9等亚型的甲型流感病毒株均有很好的检出率,灵敏度明显优于flu A-dOT和dIrECTIgEn Ez flu A;对102份入境人群鼻咽拭子标本的检测灵敏度为97.62%。结论甲型流感化学发光检测试剂具有很好的灵敏度和特异性,适用于口岸现场的甲型流感快速筛查。Objective To evaluate the sensitivity and specificity of chemiluminescence immunoassays kit for detection of influenza A virus.Methods To analyze the sensitivity and specificity of three different assay kits for detection of influenza A virus by using the viral culture liquid and nasopharyngeal swabs from entry-exit travelers.Results The chemiluminescence immunoassays kit had a good detection rate when it was tested against a panel of influenza A virus strains(H1/H3/H5/H7/H9),and its sensitivity was much better than Flu A-DOT kit`s and Directigen EZ Flu A kit' chemiluminescence immunoassays kit used for the detection of 102 nasopharyngeal swabs from entryexit travelers had a detection sensitivity of 97.62%.Conclusion Chemiluminescence immunoassays kit had good sensitivity and specificity, which was fit for the rapid detection of influenza A virus at frontier ports.国家质检总局科技计划项目(2014IK045); 厦门市科技惠民项目(3502Z20144083

窄带上行信道设计及性能测试

窄带物联网（NB-IoT）是一种具有发展前景的低功耗广域网技术,使少量的数据在相当长的时间内进行通信是NB-IoT的目标之一,也是物联网应用程序的关键特征之一。一些研究工作组已经对NB-IoT的标准进行了一些定义,例如,低功耗、广覆盖、大容量和低成本等。与原有的无线通信协议相比,NB-IoT的频谱带宽仅有180kHz,因此,如何更有效地使用资源或频谱（即资源分配和调度）是一个关键问题。文章首先对物理层上层通信进行了设计,特别是上行调度;然后,在延迟情况良好的条件下对NB-IoT的资源分配问题进行了讨论;最后,对一些开放性的问题及其对应的解决方案进行了讨论,从而保证了信号传输的可靠性

Settling with Microsoft: A Case Study of Taiwan's Political Economy and Legal Culture

[[abstract]]Antitrust challenges against Microsoft, the world’s largest software company, have raised critical legal, political, and economic issues in many countries. The purpose of antitrust law is to prevent concerted or unilateral anti-competitive behavior from harming the competitive process and thereby consumers. Nevertheless, antitrust law is economy-wide regulation, so its analysis is very fact-intensive and is on a case-by-case basis. One of the key issues in the American government’s longstanding antitrust case, for example, was whether Microsoft took exclusionary actions to maintain its monopoly in PC operating systems and to obtain a monopoly in Web browsers. Although Microsoft’s allegedly anti-competitive activities are almost identical worldwide, their legal outcomes are not. This paper will discuss the implications of the Microsoft case in Taiwan. Taiwan’s Fair Trade Commission (FTC) began an investigation against Microsoft in May 2002 after politicians and consumer organizations questioned the company’s pricing practices. Recognizing the complexity of the case, the FTC approved an administrative settlement agreement proposed by Microsoft on February 27, 2003. After giving an overview of the westernization of Chinese law and its legacy in today’s Taiwan, I will introduce the legislative background of Taiwan’s Fair Trade Act. The law, which includes antitrust provisions, was passed in 1991 and became effective on February 4, 1992. It reflected not only internal demands for promoting free competition and ending heavy-handed economic interventions from the government, but also external pressures, because other industrial countries, especially the U.S., were eager to create a level playing field in Taiwan. I will then review the FTC’s case against Microsoft and examine the settlement in a social, economic, legal, and political context. I will map some of the many influences of the settlement, including Taiwan’s intellectual property policy and consumer interests. These discussions will shed light on the rule of law in Taiwan.[[fileno]]2070402030002[[department]]科技法律研究