Intervention effect of salidroside on liver fat synthesis and oxidation of non-alcoholic fatty liver in rats induced by high-fat diet

目的:基于肝脏脂肪合成和氧化环节,探讨红景天苷防治非酒精性脂肪肝的作用机制。方法:采用单纯高脂饮食14周诱导的大鼠非酒精性脂肪肝模型。在造模第9; 周起,随机分为模型组、红景天苷组和罗格列酮对照组,灌胃给药6周。观察肝组织病理变化;肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;肝组; 织乙酰辅酶A羧化酶(ACCase)、丙二酰辅酶A (Mallonyl CoA)、脂肪酸合成酶(FAS)、肉毒碱棕榈酰转移酶-1; (CPT-1)含量的变化;肝组织ACCaseCPT-1; mRNA水平的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA、ACCase、FAS、 Malonyl; CoA含量和ACCase; mRNA水平较正常组均显著升高(P<0.01),CPT-1含量和mRNA水平较正常组显著降低(P<0.01)。红景天苷组的上述病理改变显著减轻,; 肝组织TG、FFA、ACCase、 Malonyl CoA、FAS含量和ACCase mRNA水平显著低于模型组(P<0.01) ,; CPT-1含量和; mRNA水平显著高于模型组(P<0.01)。结论:红景天苷能抑制肝脏脂肪合成,促进脂肪酸氧化,这可能是其防治非酒精性脂肪肝的重要机制。Objective: To explore the mechanism of salidroside on non-alcoholic; fatty liver disease based on liver fat synthesis and oxidation. Methods:; Non-alcoholic fatty liver disease model was induced by high-fat diet for; 14 weeks. From the ninth week, the rats were randomly divided into model; group, salidroside group and rosiglitazone group, and were given a; gavage for six weeks. The observing items including: pathological; changes of liver tissue (HE staining); changes of contents of; triglycerides (TG) and free fatty acid (FFA) in liver tissue; changes of; contents of acetyl-Coacarboxylase (ACCase), malonyl CoA, fatty acid; synthase(FAS) and carnitine palmitoyl transterase-l(CPT-l); changes of; mRNA levels of ACCase and CPT-1 in liver tissue. Results: Hepatocellular; steatosis and vacuolar degeneration were observed in the liver tissue of; the model group. The contents of TG, FFA, ACCase, Malonyl CoA, FAS and; mRNA level of ACCase in model group were significantly higher than those; of the normal group (P<0.01). The content and mRNA level of CPT-1 were; significantly lower than those of normal group (P<0.01). Hepatic; pathological changes in salidroside group were significantly reduced.; The contents of TG, FFA, ACCase, Malonyl CoA, FAS and mRNA level of; ACCase were significantly lower than those of model group (P<0.01). The; content and mRNA level of CPT-1 were significantly higher than those of; model group (P<0.01). Conclusion: Salidroside can inhibit liver fat; synthesis and promote the oxidation of fatty acid, which may be an; important mechanism of salidroside for prevention and treatment of; non-alcoholic fatty liver disease.国家自然科学基金项目; 浙江省自然科学基金项

多烯磷脂酰胆碱改善脂肪肝大鼠肝脏脂质沉积的作用机制研究

目的探讨多烯磷脂酰胆碱对高脂饮食脂肪肝大鼠肝脏脂质沉积的抑制效应及其机制。方法采用单纯高脂饮食诱导的大鼠脂肪肝模型。在造模6周后,随机分为模型组和多烯磷脂酰胆碱组,分别灌胃给予饮用水和相应药物,疗程4周,实验结束时取材观察:①肝组织病理变化(HE染色);②血清脂联素(adiponection,ADP),肝组织三酰甘油(triglyceride,TG)、游离脂肪酸(free fatty acid,FFA)、肝组织脂联素受体(adiponection receptor2,AdipoR2)、腺苷酸活化的蛋白激酶(AMP-Activated Kinase,AMPK)、脂肪酸合成酶(fatty acid synthase,FAS)、乙酰辅酶A羧化酶(acety1 CoA carboxylase,ACCase)、丙二酰辅酶A(Malony1 CoA)含量的变化及其各指标间相关性分析。结果模型组肝组织出现程度不同的肝细胞脂肪变性及空泡样变。模型组肝组织TG、FFA、FAS、ACCase、Malony1-CoA含量较正常组显著升高(P<0.01),血清ADP与肝组织AdipoR2、AMPK显著降低(P<0.01)。多烯磷脂酰胆碱组的肝脏脂肪变性显著减轻,肝组织TG、FFA、FAS、ACCase、Malony1-CoA含量显著降低(P<0.01),血清ADP与肝组织AdipoR2、AMPK含量明显升高(P<0.01)。结论多烯磷脂酰胆碱对脂肪肝大鼠肝脏脂质沉积有高强度的抑制效应,其机制与改善脂肪酸代谢有关

Intervention effect of Chinese herb components HJJB Compound on PP1-DNA-PK-USF1 signaling pathway of non-alcoholic fatty liver rats

目的:基于蛋白磷酸酶(PP1)-DNA依赖的蛋白激酶(DNA-PK)-上游刺激因子1(USF1)信号通路,探讨中药组分HJJB方防治非酒精性脂肪; 肝(NAFLD)的作用机制。方法:采用高脂饮食诱导的大鼠NAFLD模型。设模型组、HJJB方组和罗格列酮组,分别灌胃给药6周。HE染色观察肝组织; 病理变化;测定肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),肝组; 织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较正常组均明显升高(P<0.01)。HJJB方组的上述病理改变明显减轻,肝组织TG、FFA含量较模型组显著降低(P<0.0; 1),肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较模型组显著降低(P<0.01)。结论:HJJB方能显著降低脂肪肝大鼠肝组织PP1; mRNA水平和蛋白含量,进而抑制其下游信号通路,这可能是其防治NAFLD的重要机制。Objective: Toexplore the mechanism of Chinese herb component HJJB; Compound on non-alcoholic fatty liver disease based on PP1-DNA-PK-USF1; signaling pathway. Methods: SD male rats were induced by high-fat diet; for nonalcoholic fatty liver disease model. The rats were randomly; divided into model group, HJJB group and rosiglitazone group, and were; given intragastric administration for six weeks. The observing items; including: liver pathology(HE staining); hepatic triglyceride(TG) and; free fatty acid(FFA) content; mRNA and protein content changes of; hepatic protein phosphatase 1(PP1), DNA-dependent protein kinase(DNA-PK); and upstream stimulating factor 1(USF1). Results: Significant hepatocyte; fatty degeneration and vesicle changes were observed in liver tissue of; model group. The hepatic TG and FFA contents of model group were; significantly higher than those of normal group(P<0.01), and mRNA levels; and protein contents of liver tissue PP1, DNAPK, USF1 in model group; were significantly higher than those in normal group(P<0.01). Hepatic; pathological changes in HJJB Compound group were meliorated, liver; tissue TG and FFA contents of HJJB Compound group were significantly; lower than those of model group(P<0.01), and mRNA levels and protein; contents of liver tissue PP1, DNA-PK, USF1 mRNA of HJJB Compound group; were significantly lower than those of model group(P<0.01). Conclusion:; HJJB Compound can significantly decrease mRNA level and protein content; of PP1 in the liver tissue of fatty liver rats, and then inhibit the; downstream signaling pathway of PP1, which may be an important mechanism; of HJJB Compound for prevention and treatment of non-alcoholic fatty; liver disease.国家自然科学基金项目; 浙江省自然科学基金项目; 浙江省中医药科技计划项

Effect of HJJB Compound on Insulin Signal Transduction Link of Non-alcoholic Steatohepatitis Rats

目的观察红景天苷、姜黄素、绞股蓝总苷、白术多糖(HJJB)复方对非酒精性脂肪性肝炎(NASH)大鼠胰岛素信号转导环节的干预作用。方法采用高脂饮食; 14周诱导的大鼠胰岛素抵抗NASH模型。在造模第9周起,随机分为模型组、西药组(罗格列酮,0.4; mg/kg)和中药组(HJJB)。干预6周后,观察肝组织病理变化(HE染色),检测肝组织TG含量、ALT活性、血清空腹胰岛素(FINS)含量、空; 腹血糖(FBG)含量、胰岛素抵抗指数(HOMA-IR),检测肝组织胰岛素受体底物1 (IRS1 )、磷酸化IRS1 (pIRS1; )、磷脂酰肌醇-3-激酶(PI3K)、磷酸化PI3K(pPI3K)、蛋白激酵B(PKB)、磷酸化PKB(pPKB)蛋白含量;检测肝组织IRS1、; PI3K、PKB mRNA水平。结果与正常组比较,模型组出现肝细胞脂肪变性, TG、ALT、FINS、FBG 及 HOMA-IR 升高(P; <0. 01), IRS1、plRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB mRNA降低(P; <0.01)。与模型组比较,中药组和西药组上述病理改变明显减轻,血清TG、ALT、 FINS、FBG 及 HOMA-IR; 含量降低(P<0.05)。中药组 IRS1、pIRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB; mRNA水平较模型组及西药组升高(P <0.01,P <0.05),TG及ALT较西药组降低(P <0. 01; )。结论HJJB复方可上调NASH大鼠肝脏IRS1基因表达和蛋白含量,改善PI3K/PKB信号通路。Objective To observe the intervention effect of HJJB; compound(salidroside, curcumin, gypenosides and atractylodes; polysaccharides) on insulin signal transduction link of non-alcoholic; steatohepatitis (NASH) rats. Methods SD male rats were induced by; high-fat diet for 14 weeks for insulin resistance NASH model. From the; ninth week, the rats were divided into the model group, the Western; medicine(WM) group (rosiglitazone, 0. 4 mg/kg) and the Chinese medicine; (CM)group (HJJB) at random .Six weeks after medication, liver pathology; (HE staining), hepatic TG content, serum ALT activity, serum fasting; insulin (FINS), serum fasting blood glucose( FBG), insulin resistance; index (HOMA-IR) were observed. Protein content of hepatic insulin; receptor substrate insulin receptor substrate 1 (IRS1), phosphorylation; of IRS1 (pIRS1),phosphatidylinositol-3 kinase (PI3K) , phosphorylation; of PI3K(pPI3K), protein kinase B (PKB) and phosphorylation of PKB (pPKB); were detected. mRNA expression of hepatic IRS1,PI3K, PKB were also; detected. Results Significant hepatic steatosis were observed in the; model group. TG, ALT, FINS, FBG and HOMA-IR of model group were higher; than those of the normal group(P < 0. 01). Hepatic IRS1,pIRS1 ,; PI3K,pPI3K, PKB, pPKB protein expression level and IRS1 , PI3K,PKB mRNA; level were lower than those of the normal group (P <0. 01). Hepatic; pathological changes in the CM group and WB the group were meliorated,; ALT, FINS, FBG, HOMA-IR and TG of the CM group and the WM group were; lower than those of the model group(P <0. 05). Hepatic; IRS1,pIRS1,PI3K,pPI3K, PKB, pPKB protein expression level and IRS1,; PI3K, PKB mRNA of the CM group were higher than those of the model group; and the WM group(P <0. 01,P <0. 05),ALT and TG of the CM group were; lower than those of the model group (P <0. 01 ). Conclusion HJJB; Compound can significantly increase hepatic IRS1 gene expression and; protein content of fatty liver in rat, and then improve the PI3K/PKB; signal pathways.国家自然科学基金资助项目; 浙江省自然科学基金资助项目; 浙江省中医药科技计划项

Predictions of Amount of Sediment and Rate of Sediment in Watershed Using B-P Network

提出了应用 B- P人工神经网络 ,建立流域输沙量和最大输沙率的预测模型。以降雨量、降雨历时、洪峰流量和洪量等因子建立的李子溪流域的输沙量和最大输沙率的 B- P网络预测模型效果表明 :拟合率达 90 %左右 ,预留检验报准率在 75 %以上。Predictable models of amount of sediment and maximal rate of sediment in a watershed are presented using B-P artificial neural network. The predictable results of the B-P network modle developed by using area mean rainfall amount during the time of raifall,flood run off and amount of flood factor etc.It shows that the gualified rates of fitting and predicting accuracy 90% and 75%, respectively.国家自然科学基金项目! (编号 :494710 48

丹江口水库鲤肠道寄生蠕虫群落结构与季节动态

2004年2月到2005年11月在丹江口水库库区206尾鲤(Cyprinus carpio)肠道中检获蠕虫11种,其中复殖吸虫3种,线虫5种,棘头虫2种,绦虫1种。总体感染率为45.63%,平均感染丰度为4.23±12.65,平均感染强度为9.29±17.48,其中饭岛盾腹吸虫(Aspidogaster ijimai)的总感染率(25.24%)和平均感染丰度(1.76±6.46)最大,瓣睾鲫吸虫(Carassatrema lamellorchis)的感染强度(25.00±46.68)最大。除部分平均感染丰度较低的线虫如鲤带巾线虫(Cucullanus cyprini)外,其他蠕虫的分布类型均为聚集分布,蠕虫群落多样性指数为4.63,均匀度指数为0.60,对群落多样性的季节动态分析表明,各季节群落多样性和均匀度波动较大,并无明显变化规律。每尾鲤感染蠕虫种数多在1—4种之间,所有感染的11种蠕虫中优势种为饭岛盾腹吸虫;次优势种为日本侧殖吸虫(Asymphylodora japonica)、中华许氏绦虫(Khawia sinensis)、瓣睾棘吸虫和鲤长棘吻虫(Rhadinarhynchus cyprini);非优势种为对盲囊线虫(Contracaecum sp.)、鲤带巾线虫、鲤杆咽吸虫(Rhabdochona cyprini)、黄颡刺盖线虫(Spinitectus gigi)、毛细线虫(Capillaria sp.)和木村小棘吻虫(Micracanthorhynchina motomurai)。在种间协调关系方面,鲤杆咽线虫和瓣睾鲫吸虫、鲤长棘吻虫和饭岛盾腹吸虫、对盲囊线虫和木村小棘吻虫、鲤长棘吻虫和木村小棘吻虫之间分别存在显著正关联。对优势种和次优势种蠕虫中种群的季节动态分析表明,鲤寄生蠕虫各组分的感染率和平均感染丰度存在显著的季节差异,在秋、冬季节的感染水平普遍比较高,而到春夏则急剧下降,但中华许氏绦虫无显著季节变化

Research of prevention and treatment of herb components HJJB compound on non-alcoholic steatohepatitisin rats induced by high-fat diet

目的:探讨中药组分HJJB方(红景天苷、姜黄素、绞股蓝总苷、白术多糖)对高脂饮食诱导的大鼠非酒精性脂肪性肝炎的防治作用。方法:采用高脂饮食14周; 诱导大鼠非酒精性脂肪性肝炎模型。在造模第9周起,随机分为模型组,HJJB方高、低剂量组,罗格列酮组,灌胃给药6周。观察肝组织病理变化(HE染色); ,肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转肽酶(GGT)活性及TG、总胆; 固醇(TC)含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),血清ALT; 、AST、GGT活性及TG、TC含量较正常组亦明显升高(P<0.01)。HJJB方高、低剂量组的上述病理改变显著减轻,肝组织TG、FFA含量及血; 清ALT、AST、 GGT、TG、TC水平显著低于模型组(P<0.01),其中HJJB方高剂量组的肝组织TG、FFA含量和血清ALT、AST、; GGT活性显著低于HJJB方低剂量组和罗格列酮组(P<0.05,P<0.01)。结论:中药组分HJJB方对高脂饮食诱导的大鼠非酒精性脂肪性肝炎具; 有良好的防治作用。Objective: To discuss the prevention and treatment of HJJB (salidroside,; curcumin, gypenoside and atractylodes macrocephala polysaccharide); compound on non-alcoholic steatohepatitisin rats induced by high-fat; diet. Methods: Non-alcoholic steatohepatitis model was induced by; high-fat diet for 14 weeks. From the ninth week, the rats were randomly; divided into model group, HJJB high-dose group, HJJB low-dose group and; rosiglitazone group, and were given gavage for six weeks. The observing; items including: pathological changes of liver tissue (HE staining); the; changesof contents of liver tissue triglyceride (TG) and free fatty acid; (FFA); the activities of serum alanine aminotransferase (ALT), aspartate; aminotransferase (AST), glutamyl transpeptidase (GGT) and contents of; serum total cholesterol (TC) and TG. Results: Significant hepatocellular; steatosis and vacuolar degeneration were observed inthe liver tissue of; the model group. The contents of TG and FFA in liver tissue of model; group were significantly higher than those of normal group (P<0.01), and; the activities of serum ALT, AST, GGT and the contents of TG and TC of; model group were higher than those of normal group too (P<0.01). Hepatic; pathological changes in the HJJB compound high-dose, low-dose groups; were all significantly meliorated. The levels of liver TG, FFA and serum; ALT, AST, GGT, TG and TC of HJJB high-dose group, HJJB low-dose group; were significantly lower than that of model group (P<0.01). In addition,; the contents of liver TG and FFA and serum ALT, AST, GGT of HJJB; compound high-dose group were lower than those of HJJB compound low-dose; and rosiglitazone group (P<0.05, P<0.01). Conclusion: HJJB compound does; well in the prevention and treatment of non-alcoholic steatohepatitis; induced by high-fat diet.国家自然科学基金项目; 浙江省自然科学基金项目; 浙江省中医药科技计划项

Biogenic silica in surface sediments of the northeastern and southern South China Sea

本研究测定了南海东北部和南部海域表层沉积物的生物硅含量(SIO2%),其含量范围分别为1.08%—3.01%和0.79%—9.06%,平均值分别为1.76%和4.22%。研究结果表明,南海表层沉积物中的生物硅含量与站位水深呈正相关关系;南海东北部的表层沉积物中的生物硅含量与其中的矿物含量、铁离子浓度、间隙水中的营养盐浓度不存在明显的相关性;南海南部海域表层沉积物中的生物硅含量与烧失量、有机碳含量、碳酸盐含量呈正相关关系,与粘土矿物含量相关性不明显。Biogenic silica(BSi) contents in surface sediments from the northeastern South China Sea(NSCS) and southern South China Sea(SSCS) were measured.The BSi contents(SiO2%)were in the range of 1.08%–3.01% and 0.79%–9.06%,with the means of 1.76% and 4.22% for the NSCS and SSCS,respectively.The results showed that the BSi contents in surface sediments have positive correlation with water depth of the sampling locations.The BSi contents in the NSCS had no evident correlation with minerals,ferrum ion concentrations in surface sediments or nutrients in the pore waters of sediments.The BSi contents in the SSCS had positive correlations with organic material and carbonate contents but not with clay material contents.国家基础研究发展计划项目(2005CB422305

基于均匀设计的祛湿化瘀复方抗脂毒性作用的主效应中药分析

目的观察祛湿化瘀复方的主效应中药或不同组合对游离脂肪酸(free fatty acid,FFA)诱导人肝癌细胞株(HepG2)细胞脂肪沉积和肿瘤坏死因子α(turmor necrosis factor α,TNF-α)分泌的作用,探索中药复方药理作用相应物质基础的分析方法。方法采用FFA诱导HepG2细胞脂肪沉积和TNF-α分泌的体外细胞模型和药物血清技术,运用数学模型"均匀设计法",根据复方中的5味中药(茵陈、栀子、虎杖、田基黄、姜黄),选用U11(1110)表进行组方设计所得10种中药组合进行干预,以对甘油三酯(triglyceride,TG)及TNF-α的抑制效应作为考察指标,筛选主效应中药或组合,并重新区间分组验证。结果茵陈和田基黄在高剂量组合时有显著降低细胞TG及TNF-α含量的效应,与全方比较差异无统计学意义,其中单用茵陈也可显著降低细胞TG及TNF-α含量。结论茵陈及其与田基黄的组合是祛湿化瘀复方抑制FFA诱导HepG2细胞脂肪沉积和TNF-α分泌作用的主效应中药;应用均匀设计与药效学分析的方法可有效分析中药复方针对某一作用环节的主效应中药或组合

The Acetylation of Thymosin α1 is Independent on RimL in Escherichia coli

目的:考察大肠杆菌乙酰转移酶rIMl对胸腺素α1(Tα1)乙酰化修饰的影响。方法:构建含500bP同源臂的卡那抗性基因打靶片段,利用rEd同源重组系统,使大肠杆菌bl21(dE3)的rIMl基因插入失活,随后导入质粒PCP20去除抗性基因,构建突变菌株rIMl-bl21(dE3);将重组质粒PET-Tα1-l12分别转入出发菌株和突变菌株中进行表达,经固定金属离子亲和层析和反向高效液相层析后,将所得纯品进行质谱分析,精确测定相对分子质量。结果:PCr鉴定结果证明成功敲除rIMl基因;质谱结果表明,rIMl基因敲除菌中所表达的Tα1-l12融合蛋白与出发菌株一样,均有部分乙酰化修饰。结论:Tα1的乙酰化修饰并不依赖于rIMl。Objective:To investigate the effect of RimL,a N-terminal acetyltransferase of E.coli,on thymosin α1(Tα1) acetylation.Methods:A kanamycin cassette with two 500 bp long arms homologous to the regions around rimL as the replacement fragment was electroporated into cells,in which the Red recombinant functions was induced.Subsequently,the plasmid pCP20 was transformed to eliminate the kanamycin resistant gene.The plasmid pET-Tα1-L12 was transformed into the original and mutant strains respectively.The expressed fusion protein Tα1-L12 was purified by IMAC and RP-HPLC.The accurate molecular weight of the fusion protein was measured by Q-Tof-MS.Results:The gene rimL was inactivated by insertion of the replacement fragment successfully.According to the MS results,the fusion protein Tα1-L12 was still partly acetylated when it was expressed in the mutant strains as in the original strains.Conclusion:Tα1 acetylation was independent on RimL