Classification Analysis Based on Literature about TCM Syndrome Types of Depressive Disorder

在遵循循证医学原则的前提下,系统检索近10年来抑郁症中医及中西医结合临床研究类文献。并将检索的文献按照证候研究、方药治疗、辨证论治、综述进行分类,根据各类文献的研究特点采用不同的统计方法,对抑郁症证候分类进行分析研究,以期总结归纳出抑郁症临床常见证候,为抑郁症中医证候规范化、客观化提供参考依据。统计分析结果显示抑郁症临床常见证候依次为:肝气郁结、肝郁脾虚、心脾两虚、痰气郁结、肝郁肾虚、气郁化火。On the premise of following evidence-based medicine,we searched the clinical research literature of traditional Chinese medicine( TCM) and combined traditional Chinese and western medicine on depressive disorder in nearly a decade comprehensively.We classified these literature according to the syndrome types research,clinical therapy,syndrome differentiation and treatment and literature review.On the basis of the characteristics of literature and different statistical methods,we analyzed the syndrome classification of depressive disorder to summarize the common syndrome types of clinical depressive disorder,providing some references for the standardization and objectification of syndromes for depressive disorder.Statistical analysis results show that the depressive disorder common clinical syndromes in the order:stagnation of liver qi,stagnation of liver qi and spleen deficiency,heart-spleen deficiency,phlegm-qi stagnation,liver depression and kidney deficiency,qi depression transforming into fire.福建省自然科学基金项目(2014J01374);; 厦门市科技计划项目(3502Z20133007

均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢异常的效应组分

目的:运用均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢紊乱的效应组分及其最优剂量配比,探讨揭示复方配伍理论科学内涵的研究方法。方法:将茵陈蒿汤(茵陈、栀子、大黄)进行分组设计,分为6种中药组合,对高脂饮食诱导的大鼠脂肪肝模型进行干预,观察各组大鼠肝脏的病理变化,并以血清三酰甘油(TG)、肝组织TG、血清低密度脂蛋白(LDL)测定作为筛选指标,分析不同作用途径的效应组分及其最优剂量配比。结果:较之正常组,模型组血清TG、LDL和肝组织TG含量均明显升高(P<0.05~0.01),肝组织存在明显脂肪变性;各药物组上述脂肪病理改变程度均明显减轻。根据均匀设计实验逐步回归分析,降低血清TG和降低肝组织TG的效应组分分别是茵陈、栀子。而茵陈与大黄对降低血清LDL有明显疗效,以茵陈0.3g+大黄0.15g为最优剂量组合。结论:茵陈蒿汤通过多途径调节脂肪肝的脂质代谢,其不同作用途径存在特定的效应组分及相应的最优剂量配比

柴郁地仙方治疗围绝经期抑郁症体会

围绝经期抑郁症的发病基础是肾虚精血亏少,七情过极是诱因,病程中多表现为肝郁气滞、痰瘀交阻、闭塞脑窍。肾虚肝郁又常累及心脾二脏,故以补肾疏肝、化痰祛瘀、补益心脾立法,自拟柴郁地仙方治疗本病取得良好效果。从实验及临床研究结果来看,补肾疏肝、化痰祛瘀、补益心脾这种多脏腑整体调治,虚实兼顾治疗围绝经期抑郁症的思路较有效,且值得进一步推广和深入探讨。福建省卫生厅资助项目(WZPW101307);; 厦门市科技局资助项目(3502Z20133007

Three-Dimensional Fluorescence Technique Coupled with Chemometric Second-Order Calibration Method for Simultaneous Detection of Thiabendazole and Fuberidazole in Red Wine

噻菌灵和麦穗宁是被广泛应用于预防治疗蔬菜水果病害的苯并咪唑类杀菌剂,二者的荧光光谱重叠严重,常规的荧光方法难以实现同时直接定量分析。本文将激发发射三维荧光技术分别与平行因子分析(PArAfAC)算法和交替三线性分解(ATld)算法相结合直接用于定量分析红葡萄酒中噻菌灵和麦穗宁的残留含量。当体系主成分数为3时,ATld和PArAfAC获得预测样品中噻菌灵和麦穗宁的平均回收率分别为(105.8±2.7)%、(102.1±10.1)%和(102.4±7.6)%、(103.1±9.5)%。本方法可简单、快速、经济、同时地分析红葡萄酒中噻菌灵和麦穗宁含量。Thiabendazole(TBZ) and fuberidazole(FBZ) are a class of benzimidazole fungicides and have been widely applied to protect vegetables and fruits.Conventional fl uorescence spectrum analysis method can hardly simultaneously detect TBZ and FBZ due to their serious spectral overlap.The simultaneous determination of TBZ and FBZ in red wine by excitation-emission fluorescence matrices(EEMs) combined with parallel factor analysis(PARAFAC) and alternating tri-linear decomposition(ATLD) was developed in this study.The prediction recoveries of TBZ and FBZ in the prediction set by PARAFAC and ATLD were(105.8±2.7)%,(102.1±10.1)% and(102.4±7.6)%,(103.1±9.5)% respectively,when the principal component number was 3.The method is simple,fast,low-cost,and suitable for the simultaneous determination of TBZ and FBZ in red wine.国家自然科学基金项目(21127005;21375111); 福建省科技重点项目(2009Y0046

栀子有效成分栀子苷的现代研究进展

目的对栀子苷的现代研究进展予以综述,以期为栀子苷的进一步研究及应用提供参考。方法综合近年来的相关文献,从栀子苷的分离提取工艺及含量测定、药理学与毒理学、药物代谢动力学等方面进行概述。结果栀子苷存在广泛的药理作用,但也有一定的毒性作用。结论有必要进一步探索栀子苷的用量控制和吸收方式以及毒性和保护作用的确切机制,运用现代科学的研究方式对其进行合理的利用与开发

栀子与茵陈蒿汤对非酒精性脂肪性肝病大鼠脂质代谢及血清酶学影响的比较

目的:观察比较栀子与茵陈蒿汤对非酒精性脂肪性肝病大鼠脂质代谢及血清酶学的影响。方法:用高脂饮食建立大鼠非酒精性脂肪肝模型,自造模第6周起,28只大鼠被随机分为模型组、栀子组、茵陈蒿汤组和易善复组,每组7只,灌胃饮用水或给药5周。通过肝脏组织HE染色观察肝组织病理学;肝功酶试剂盒及血脂试剂盒检测大鼠血清生化指标及肝组织甘油三酯(TG)含量。结果:模型组大鼠肝脏存在明显脂肪变性,并可见炎症细胞浸润,肝组织TG、血清总胆固醇(TC)、甘油三酯(TG)含量、血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)活性均显著升高(P<0.01),血清高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.01);栀子组、茵陈蒿汤组大鼠肝脂肪变性及炎症反应较模型组明显减轻,肝组织TG、血清TG、TC含量、血清ALT、AST活性均较模型组显著降低(P<0.05,P<0.01),血清HDL-C则显著升高(P<0.01);栀子组在降低血清ALT及血清TC含量方面优于茵陈蒿汤组(P<0.05,P<0.01)。结论:栀子与茵陈蒿汤均能明显改善脂肪肝大鼠肝组织病理学变化,对非酒精性脂肪性肝病有较好的干预作用,而栀子在改善胆固醇代谢及抗炎症损伤方面,效果优于茵陈蒿汤

Effect of geniposide on the lipid metabolism and inflammatory injury of nonalcoholic fatty liver disease in the hamster

目的:观察栀子苷对高脂饮食诱导的金黄地鼠非酒精性脂肪性肝病模型脂质代谢、肝组织病理变化及炎症损伤的影响,并与栀子进行比较,以明确栀子抗脂肪肝作用的效应成分。方法:24只雄性金黄地鼠按随机数字表法分为正常组、模型组、栀子组和栀子苷组。实验后观察肝组织病理学变化,并检测肝组织Tg含量、血清Tg、CHO含量及AlT、AST活性。结果:与模型组比较,栀子组、栀子苷组肝Tg含量、血清Tg、CHO含量及AlT、AST活性均显著降低,且栀子苷对AlT、AST活性的降低作用优于栀子。结论:栀子苷与栀子对高脂饮食诱导的地鼠脂肪肝均有显著干预作用,且栀子苷抗炎症损伤作用优于栀子。Objective: To observe geniposide's effect on lipid metabolism,hepatic tissue pathological change and inflammatory injury of golden hamsters with nonalcoholic fatty liver disease( NAFLD),caused by high fat diet.Compare the geniposide's effect with gardenia to make clear the effective components in Gardenia that can resist fatty liver.Methods: 24 male golden hamsters were averagely divided into control group,model group,gardenia group and geniposide group.The experiment was based on building the model,and then observe hamsters 'general condition and pathological change of hepatic tissue,examine the content of hepatic tissue TG,serum TG,CHO and the activity of ALT,AST.Results: In gardenia group and gardenoside group,The content of hepatic tissue TG,serum TG,CHO and the activity of ALT,AST are significantly lower than the model group.Besides,gardenoside can lower the activity of ALT,AST better than gardenia.Conclusion: Both gardenoside and gardenia have notable intervention effects on hamsters' NAFLD caused by HFD and gardenoside have better effects on resisting inflammatory injury than gardenia,which suggested that gardenoside was the primary effective component in resisting NAFLD.国家自然科学基金面上项目(81274155); 厦门市重大科技计划项目(3502Z20100006

茵陈蒿汤抗游离脂肪酸对HepG2细胞脂毒性作用的效应中药研究

目的:探讨对游离脂肪酸(FFA)诱导人肝癌细胞株(HepG2)细胞脂肪沉积和对肿瘤坏死因子(TNF-α)分泌有显著抑制作用的茵陈蒿汤的效应中药或组合。方法:采用FFA诱导HepG2细胞脂肪沉积和TNF-α分泌的体外细胞模型和药物血清技术,运用数学模型"均匀设计法",根据复方中的3味中药(茵陈、栀子、大黄),选用U7(76)表进行组方设计所得6种中药组合进行干预,以对甘油三酯(TG)及TNF-α的抑制效应作为考察指标,筛选效应中药或组合,并重新区间分组验证。结果:茵陈和栀子在高剂量组合时有显著降低细胞TG及TNF-α含量的效应,与全方无显著性差异,其中单一茵陈也可显著降低细胞TG及TNF-α含量。结论:茵陈及其与栀子的组合是茵陈蒿汤抑制FFA诱导HepG2细胞脂肪沉积和TNF-α分泌作用的效应中药

Jiangzhi and Hepatoprotective effect of salidroside on nonalcoholic fatty liver disease

目的:探讨中药红景天的主要成分红景天苷对高脂饮食诱导的金黄地鼠非酒精性脂肪性肝炎(nASH)的干预作用。方法:18只雄性金黄地鼠随机分成正常组、模型组和红景天苷组,除正常组外,其余两组均给予高脂饮食喂养6周以复制nASH模型;造模第3周起,红景天苷组给予红景天苷药液灌胃4周。6周后处死地鼠,收集标本,检测肝组织甘油三酯(Tg)含量,血清天门冬氨酸氨基转移酶(AST)活性,对肝组织进行HE染色。结果:红景天苷能明显降低肝组织Tg含量及血清AST活性,改善肝组织脂肪变性的病理状态。结论:红景天苷具有显著的抗nASH病理损伤的药理效应。Objective:To explore the intervening effect of the main components of the rhodiola on the nonalcoholic fatty liver disease(NAFLD) for male golden hamsters induced by high-fat diets.Methods:Total 18 male golden hamsters were divided into control group,model group and salidroside group randomly.Male golden hamsters in the model group and salidroside group were fed with high fat diet for 6 weeks to reproduce NAFLD model.After the second week of feeding,male golden hamsters in the salidroside group were given a gavage of salidroside.All male golden hamsters were put to death at the 6 weeks and samples were collected.Hepatic tissue TG contents were detected.Serum AST activities were determined.Hepatic tissues were detected by HE staining.Results:Salidroside decreased hepatic tissue TG contents,serum AST activities and reduced the fatty degeneration in hepatocytes significantly.Conclusion:Salidroside has conspicuous pharmacological effect on the nonalcoholic fatty liver disease in male golden hamsters.国家自然科学基金(No.81274155); 厦门市重大科技计划项目(No.3502Z20100006); 横向课题(No.XDHT2011366A)~