Financial Development,Transport Facilities and Household Consumption-Findings from Additive Semi-Parametric Model

本文利用半参数可加模型实证分析了金融发展水平、交通设施条件与中国居民消费的关系,实证结果表明:(1)金融系统的规模扩张与居民消费存在显著的正向线性关系,而金融系统效率的改善对居民消费则主要表现为倒“u“型的非线性影响。(2)公共交通和道路设施条件与居民消费的线性关系不同,前者为正,而后者为负,但两者的非线性影响大体一致,均表现为“n“型趋势。并对如何提升居民消费水平提出了相应的政策建议。This paper analyzes the relationship between financial development,transport facilities and China's household consumption using non-parametric model.Empirical results show that:(1) Expansion of the scale of financial system and household consumption has a significant positive linear relationship,and the nonlinear effect of efficiency improvement of financial system on household consumption has an inverted "U" shape.(2) The relationship between public transport and road facilities and household consumption are different,the former is positive,while the latter is negative.However,nonlinear effects of the above two are much the same and both have an "N" shape trend.Based on empirical results,this paper finally proposes some policy recommendations to raise the level of household consumption.国家社科基金项目“非参数空间计量经济模型及应用研究”(10CTJ002);中央高校基本科研业务经费项目“动态时变特征的空间计量模型及其在中国地方政府财税策略互动中的应用”(ZK1021

上证行业指数收益率的影响因素——基于三因素模型的分位数回归分析

fAMA And frEnCH(1993)提出的三因素模型对许多国家的股市收益率有良好的解释力并得到广泛应用。我们在三因素模型的基础上,利用分位数回归技术全面考察三因素对上证行业指数收益率的影响。实证结果表明:(1)在各个分位点上,市场因子对五类行业指数均有显著的正向影响;(2)规模因子对五个行业指数的影响方向不同,其中对综合指数和地产指数具有显著的负向影响,而对其他三类指数具有显著的正向影响;(3)账面市值因子对五个行业指数的影响方向也不同,其中对综合指数和公用指数具有负向影响,对工业指数和商业指数具有正向影响,对地产指数既有正向影响又有负向影响,同时在部分分位点上,账面市值因子对五个行业指数的影响不显著。国家社科基金项目“非参数空间计量经济模型及应用研究”(10CTJ002);中央高校基本科研业务经费项目“动态时变特征的空间计量模型及其在中国地方政府财税策略互动中的应用”(ZK1021)的部分研究成

数值摄动差分法对有源对流扩散方程计算精度的提高

对流扩散方程中函数源项的存在能使许多格式产生假扩散，许多格式计算有源对流扩散方程所产生的数值误差比计算齐次对流扩散方程的误差要大很多。本文研讨高智数值摄动差分法能否减轻上述假扩散的问题；通过对流项和源项同时摄动，摄动后格式的整体精度与未摄动格式相比有了很大的提高，这是摄动格式较其他格式的一条优良特性；本文最后通过数值实验证实了数值摄动差分法的这条优良特性。由于具有工程价值的对流扩散型方程（例如Navier-stokes 方程组）都是有源的，因此将数值摄动差分法的这条优良特性应用到工程计算中去将会产生重要的实用价值

综合化学新实验——手性席夫碱Ni(Ⅱ)络合物的合成与表征

本实验属于化学系综合化学实验,其构思来自手性络合物的立体选择性合成。学生可通过本实验从实践和理论上探讨手性配体在立体选择性合成手性络合物中的作用并掌握对手性络合物组成和结构的各种表征方法特别是CD光谱方法

Redistribution of iron during directional solidification of metallurgical-grade silicon at low growth rate

研究冶金级硅低速定向凝固过程中杂质铁的再分布。采用ICP-MS检测铁的浓度,绘制铁在固相、液相、晶界和晶体生长方向上的浓度示意图。铁浓度在固、液相中具有明显差异。依据质量守恒定理,计算得出溶质边界层约为4 mm,铁的有效分凝系数约为2.98×10~(-4)。在低速凝固条件下,铁容易偏析聚集在晶界。在生长方向上,由于低速凝固,铁浓度在硅锭86%高度以下几乎恒定,不完全符合Scheil方程规律。讨论了对流对铁再分布的影响,对流"死区"对铁再分布具有重要影响。Redistribution of iron during directional solidification of metallurgical-grade silicon(MG-Si) was conducted at low growth rate. Concentrations of iron were examined by ICP-MS and figured in solid and liquid phases, at grain boundary and in growth direction. Concentrations are significantly different between solid and liquid phases. The thickness of the solute boundary layer is about 4 mm verified by mass balance law, and the effective distribution coefficient is 2.98×10~(-4). Iron element easily segregates at grain boundary at low growth rate. In growth direction, concentrations are almost constant until 86% ingot height, and they do not meet the Scheil equation completely, which is caused by the low growth rate. The effect of convection on the redistribution of iron was discussed in detail. Especially, the "dead zone" of convection plays an important role in the iron redistribution.Projects(51334004,51204143)supported by the National Natural Science Foundation of China;; Project(2006L2003)supported by the Scientific Technological Innovation Platform of Fujian Province,Chin

Expression of Fusion Protein of Parathyroid Hormone and Transferrin N-terminal Half-molecule in Pichia pastoris

利用重叠PCR技术将PTH(parathyroidhormone,甲状旁腺激素)基因与TFN(transferrinN_terminalhalf_molecule,转铁蛋白N端半分子)基因在体外融合,融合基因克隆至真核表达载体pPIC9中,转化毕赤酵母GS115。转化子经甲醇诱导后,融合蛋白得到了表达并分泌到发酵上清液中。经SPSepharoseFF阳离子交换层析、PhenylSepharoseFastFlow疏水层析纯化获得了纯度大于95%的PTH_TFN样品。Westernblot分析及腺苷酸环化酶实验证明融合蛋白中的PTH具有与抗PTH抗体结合能力及刺激腺苷酸环化酶的活性,铁饱和实验证明融合蛋白中的TFN和单独的TFN具有相同铁结合能力。因而TFN可望作为PTH的天然运输载体。The fused gene (PTH_TFN) of parathyroid hormone (PTH) gene and transferring N_terminal half_molecule (TFN) gene was amplified by multiple PCR and inserted into pPIC9 vector. The recombinant plasmid pPIC9_PTH_TFN was transformed into Pichia pastoris GS115 by PEG. After methanol induction, the target protein was expressed in fermentation supernatant at high level.The fused protein PTH_TFN with purity being higher than 95% was finally obtained after purification through two_step chromatography : SP Sepharose Fast Flow and Phenyl Sepharose Fast Flow.Western blot analysis and adenylate cyclase assay proved that the fused protein exhibited the bioactivity to stimulate cAMP synthesis and the ability to bind Fe ~3+ in the Fe ~3+ saturation study as the recombinant TFN did indicating that TFN could be used as the transcellar carrier of PTH.国家高技术研究与发展项目基金资助(No.2004AA215172)。~

Mirror Symmetry Breaking of cis-[Ni(NCS)_2tren]: Special Chiral Conformations of Chelate Rings

为了探究CIS-[nI(nCS)2TrEn][TrEn:三(2-氨基乙基)胺]的手性来源,本文采用单晶X射线衍射、溶液紫外-可见-近红外(uV-VIS-nIr)光谱、固体紫外圆二色(Cd)光谱和粉末X射线衍射(Xrd)等对CIS-[nI(nCS)2TrEn]的一对手性晶体进行了表征.研究结果表明:该手性晶体由结晶过程中的镜面对称性破缺而形成;三角架型配体TrEn配位后的特殊手性构象(δδλ,λλδ)是CIS-[nI(nCS)2TrEn]的主要手性来源.络合物固体紫外Cd谱所呈现的COTTOn效应可能来自其螯环手性构象以及手性金属中心对nCS-配体的π-π*跃迁和荷移跃迁生色团的手性微扰.对20批次合成产物进行固体Cd检测的统计结果表明:它们的对映体过量(EE)值在39%-100%之间.In order to explore the chiral origin of cis-[Ni(NCS)2tren] [tren: tris(2-aminoethyl) amine], a pair of chiral crystals of cis-[Ni(NCS)2tren] was characterized by X-ray single crystal structural analysis, solution UV-Vis-near infrared (NIR) spectroscopy, solid state UV-circular dichiroism (CD), and powder X-ray diffraction (XRD) spectra.The results indicated that the chiral crystals of cis-[Ni(NCS)2tren] were obtained by mirror symmetry-breaking crystallization, and the special chiral ring conformations (δδλ, λλδ) of the coordinated tripod-type tren ligands are responsible for the chiral origin of cis-[Ni(NCS)2tren].The Cotton effects of Ni(II) complexes in the solid-state UV-CD spectra are presumably attributed to the π-π* and charge-transfer chromophores of the NCS- ligands by the chiral perturbation of the helical ring conformations and metal-centered chirality.According to the statistical results of solid-state CD spectra of cis-[Ni(NCS)2tren] for twenty batch syntheses, their enantiomeric excess (ee) values are between 39% and 100%.国家自然科学基金(20773098;20973136)资助项目---

cis-[Ni(NCS)_2tren]的镜面对称性破缺: 螯环的特殊手性构象

为了探究cis-[Ni(NCS)2tren][tren:三(2-氨基乙基)胺]的手性来源,本文采用单晶X射线衍射、溶液紫外-可见-近红外(UV-Vis-NIR)光谱、固体紫外圆二色(CD)光谱和粉末X射线衍射(XRD)等对cis-[Ni(NCS)2tren]的一对手性晶体进行了表征.研究结果表明:该手性晶体由结晶过程中的镜面对称性破缺而形成;三角架型配体tren配位后的特殊手性构象(δδλ,λλδ)是cis-[Ni(NCS)2tren]的主要手性来源.络合物固体紫外CD谱所呈现的Cotton效应可能来自其螯环手性构象以及手性金属中心对NCS-配体的π-π*跃迁和荷移跃迁生色团的手性微扰.对20批次合成产物进行固体CD检测的统计结果表明:它们的对映体过量(ee)值在39%-100%之间

The Acetylation of Thymosin α1 is Independent on RimL in Escherichia coli

目的:考察大肠杆菌乙酰转移酶rIMl对胸腺素α1(Tα1)乙酰化修饰的影响。方法:构建含500bP同源臂的卡那抗性基因打靶片段,利用rEd同源重组系统,使大肠杆菌bl21(dE3)的rIMl基因插入失活,随后导入质粒PCP20去除抗性基因,构建突变菌株rIMl-bl21(dE3);将重组质粒PET-Tα1-l12分别转入出发菌株和突变菌株中进行表达,经固定金属离子亲和层析和反向高效液相层析后,将所得纯品进行质谱分析,精确测定相对分子质量。结果:PCr鉴定结果证明成功敲除rIMl基因;质谱结果表明,rIMl基因敲除菌中所表达的Tα1-l12融合蛋白与出发菌株一样,均有部分乙酰化修饰。结论:Tα1的乙酰化修饰并不依赖于rIMl。Objective:To investigate the effect of RimL,a N-terminal acetyltransferase of E.coli,on thymosin α1(Tα1) acetylation.Methods:A kanamycin cassette with two 500 bp long arms homologous to the regions around rimL as the replacement fragment was electroporated into cells,in which the Red recombinant functions was induced.Subsequently,the plasmid pCP20 was transformed to eliminate the kanamycin resistant gene.The plasmid pET-Tα1-L12 was transformed into the original and mutant strains respectively.The expressed fusion protein Tα1-L12 was purified by IMAC and RP-HPLC.The accurate molecular weight of the fusion protein was measured by Q-Tof-MS.Results:The gene rimL was inactivated by insertion of the replacement fragment successfully.According to the MS results,the fusion protein Tα1-L12 was still partly acetylated when it was expressed in the mutant strains as in the original strains.Conclusion:Tα1 acetylation was independent on RimL

Cu-Zn-Al-Zr甲醇合成催化剂的UVDR、TPD和TPR表征

在 Cu- Zn- Al甲醇合成催化剂中添加适量的氧化锆助剂 ,制得 Cu- Zn- Al- Zr催化剂及表征其特性 .实验结果表明 ,Cu- Zn- Al- Zr催化剂最佳反应温度为 2 30℃ ,比 Cu- Zn- Al低约 10℃ ;热处理前后甲醇得率分别比 Cu- Zn- Al高 16%和 38% .UVDR表征显示 ,反应后催化剂在 5 98nm处观测到在 Zn O晶格介面上的 Cu+;CO- TPD表征显示 ,经 4 5 0℃热处理后的催化剂只在 182℃～ 197℃处出现一个由 Cu0 位贡献的吸附峰 ,热处理前在 2 61℃～ 2 74℃处由 Cu+位贡献的吸附峰消失 .Cu-Zn- Al- Zr催化剂对 CO的吸附量和 Cu+含量均大于 Cu- Zn- Al催化剂 ,这与该催化剂具有较好的低温活性和较高的热稳定性密切相