银鲫遗传型雄性和温度依赖型雄性的比较研究

在变温动物中，不同的性别决定机制可以在同一物种中共存并且发生相互转变，但由不同性别决定机制造成的雄性之间的差异及其内在机制还不清楚。在本研究中，我们利用同时具有遗传型性别决定和温度依赖型性别决定的雌核生殖银鲫（Carassius gibelio）来阐述这个问题。 通过本文的研究发现，银鲫遗传型雄性和温度依赖型雄性具有相似的雄性形态、精巢组织结构、精子结构和精子活力。然而，当雌性个体与遗传型雄性受精时，在受精卵中可以观察到精核发生解凝并与雌原核融合。与此相反的是，当雌性个体与温度依赖型雄性受精时，精核在整个受精过程中一直保持凝缩的状态。对两种雄性进行精液蛋白质组分析表明，相比于遗传型雄性，温度依赖型雄性的精子中DNA复制通路和基因表达相关的通路是被抑制的，大多数与DNA复制、转录和翻译相关的差异表达蛋白是下调的。随后，BrdU掺入实验和免疫荧光检测证实了在受精过程中遗传型雄性的精核的DNA发生了复制，而温度依赖型雄性精核的DNA没有发生复制。 这些结果表明，当雌性与遗传型雄性和温度依赖型雄性受精时，在受精卵中会出现不同的精核发育行为，这种差异与DNA复制及基因表达相关通路有关。本研究是在雌核生殖物种中首次尝试揭示遗传型性别决定和温度依赖型性别决定所产生的雄性之间的差异，我们的工作为理解单性脊椎动物不同性别决定机制在进化中的适应性提供了线索

福建水库空间分布特征:沿海密度高水量少、内陆密度低水量多

水库是福建重要的水资源.通过2013-2015年遥感影像结合Google Earth和天地图提取福建水库3353座,分布在81个县区,总面积647.51 km2,约占全省土地面积的0.5%;其中面积≤1 km2水库3248座,总面积197.16 km2,面积>1 km2水库105座,总面积450.35 km2.基于经验公式估算总蓄水量188.18亿m3,其中小型水库3078座(91.80%),蓄水总量37.06亿m3(19.69%),大中型水库275座(8.20%),蓄水总量151.12亿m3(80.31%).从空间分布格局来看,福建水库水资源空间分布不均,沿海六市水库密度大于三个内陆市,大中型水库主要分布于福建西北部,蓄水量呈现西北多、东南少的特点.单位陆地面积水库数量沿海城市县区均多于内陆,而单位人口水库数量则相反;单位面积水库蓄水量沿海与内陆差异不大,而人均蓄水量则沿海大部分县区远小于内陆.仅以水库作为供水水源,不能满足沿海地区用水,但内陆地区供水充足.水库蓄水对河流水体的平均滞留时间为0.053~0.341 a,除晋江流域受水库蓄水强烈影响外,其他流域受水库中等程度影响.国家自然科学基金项目(31370471);;福建省杰出青年科学基金项目(2012J06009)联合资

Design and Implementation of Vehicle Permit Management System for Urumqi City Traffic Police Detachment

由于乌鲁木齐车辆数量大，道路狭窄，为了缓解交通压力，市交警支队采用了白天限制货运车辆通行的措施，针对白天有紧急事务的货运车辆必须通行的情况，采用了发放车辆通行证的办法解决，发放了车辆通行证的货运车辆白天可以在指定路段通行，但由于乌鲁木齐市道路数量大，交警大队数量多，在实际管理中存在各交警大队滥发车辆通行证的情况，有的车辆分别在不同大队分别申请通行证，使得白天限制货运车通行的措施有名无实。为了加强通行证管理必须采取全市统一办理货运车辆通行证，对通行证办理进行严格审批。 在对交通科进行了调研的基础上，设计了数据库概念模型，将通行证的若干道路用一个字段存储，解决了通行证和路段间的多对多的复杂关系，...As a result of Urumqi vehicle quantity is big and the narrow road, city traffic police detachment used a daytime restricted freight traffic measures in order to alleviate the traffic pressure. For daytime emergency freight vehicles must pass the issuing vehicle permit solution. With the vehicle passes the freight vehicles during the day can be specified in road traffic. Because of large quantity o...学位：工程硕士院系专业：软件学院_工程硕士(软件工程)学号：X201023027

Flow ofImage:researchonrelations between TV Media Culture and Postmodernism thoughts

“后现代”这一论题，自上世纪八十年代始，成为西方学界争议的中心。当代西方不同学科领域的学者纷纷卷入其中，最有名的当属法国的利奥塔与德国的哈贝马斯之间的争论，美国的贝尔、罗蒂，法国的鲍德里亚、英国的鲍曼等大师级学者也直接间接地参与或对此争论有所回应。抛开人们加之于“后现代”的毁誉褒贬不论，这一思潮已经波及并正在渗透几乎每一个人文社会科学领域，则是不争的事实。而在我国，有关后现代的讨论也延续了二十余年，至今热度未见衰减——不包括专著，各类学术期刊上与“后现代”相关的论文每年至少数以千计。后现代现象的出现，与社会经济的结构变迁有着十分密切的关联。相当多的理论家把它看作是后工业社会或者消费社会在文化领...The term “postmoderism” has became the centre theme disputed by the west academy since 80s of 20th century. Contemporary western scholars in different disciplinary fields involved in it, especially the most famous argument between French Lyotard and German Hahermas,at the same time many masters, such as American Bell, French Baudrillard, and British Bauman and so on, joined in the dispute directly...学位：文学博士院系专业：人文学院中文系_文艺学学号：B20040100

美学研究的范式转换:从主体性到主体间性

实践美学对主体性的强调,是美学研究的一大理论收获,也在文艺创作和审美实践中起到了积极的指导作用。但是,主体论哲学背后理性独断论的理论误区,使实践美学面临着自身难以克服的矛盾和局限。后实践美学关注主体间性在审美活动中的本体地位,既为方兴未艾的生态美学提供了坚实的基础,又沟通了中国传统美学思想,从而拓展了美学理论的阐释空间

我国货币供应量、GDP增长与通货膨胀的发展变化关系

本文系统分析了我国货币供应量、GDP增长以及通货膨胀这三者之间的关系,并依据协整理论建立了反映三者发展变化关系的误差修正模型(ECM)以及向量误差修正模型(VEC)。通过对模型的分析得出结论:这三者之间存在着短期的波动与长期的均衡关系

Research on Dynamic Raman Detection for Cell and Biomolecule and Its Key Technologies

细胞和生物分子领域的发展对研究技术和手段的一个迫切需求，就是要解决生命过程的动态检测问题，才能更深一步探究细胞和生物分子中伴随动态过程的一系列物质和能量的变化，对医学或生物分子学的发展具有重大意义。另一方面，拉曼光谱作为在分子水平上反映物质组成的“指纹”信息，在细胞和生物分子领域的应用日趋广泛；而表面增强拉曼技术（SERS）的发展，使得具有表面增强拉曼效应的金属纳米粒子在相关领域的应用日益受到人们关注。 将表面增强拉曼技术应用于细胞和生物分子的动态检测，是解决医学或生物学中对动态生命过程进行研究的一个重要方法，也是“973”项目“适合于细胞和生物分子动态监测的高时空分辨拉曼光谱技术”的重要目...With the development of cell and biomolecule field, technique and method for researching are being put into a higher level. Its sole purpose has been to find solutions to the problem of dynamic detection in their process of life. Then further exploration of a series of changes in material and energy of cells and biomolecule can be gone on. And it is great significant for the development of medical...学位：工学硕士院系专业：物理与机电工程学院_测试计量技术及仪器学号：1992011115281

The Realistic Significance of Scientific Outlook to Path Selection in Communications Localization——Reemphasizing the Concept of Development Communications

"传播学本土化"是一直困扰我国传播学研究的一个老问题。尽管备受争议,但是这个问题的理论合法性并未被撼动。然而如果因循现在的研究思路,孜孜于传统典籍之中搜寻本土资源,可能永远也达不到建立中国传播学或传播学中国学派的目标。因此,传播学本土化研究必须进行范式转换,借鉴发展传播学的理论资源,以传播与中国的社会发展为切入点,在现实中建立传播学的"问题意识",从而推动中国传播学研究走出困境,开创新局。"Communications Localization" has been a heated topic in the China's Communications field for years. Despite its controversy, the legitimacy of Communications Localization has never been shaken. Nonetheless, if we follow current research pattern, i.e., researching local resources from ancient books and literatures, we may never reach the goal of establishing China's Communications or Chinese School of Communications. Therefore, the research pattern of Communications Localization needs to be reformed. By referring to theoretical resources of Development Communications, taking the cutting point of Communication and social development in China, we can develop the "problem awareness" within the real world, so as to pull Communications research out of its current dilemma, and blaze a trail of its own

Circular RNA CircHIPK3 Promotes NCI-H1299 and NCI-H2170 Cell Proliferation through miR-379 and its Target IGF1

背景与目的已有的研究证明:环状RNA是一类在哺乳动物中普遍存在的具有稳定闭合环状结构的内源性RNA分子。环状RNA; circHIPK3(circular RNA HIPK3, circHIPK3)在肝细胞癌(hepatocellular carcinoma,; HCC)中表达水平较高,促进肝癌细胞生长。但是其在非小细胞肺癌(non-small cell lung cancer,; NSCLC)中的作用及其调控机制尚无文献报道。本研究拟探讨环状RNA; circHIPK3对NSCLC细胞系NCI-H1299和NCI-H2170细胞增殖的影响,并进一步研究其调控的分子机制。方法Real-time; PCR法检测circHIPK3在NSCLC各细胞系中的表达水平。CCK-8实验和克隆形成实验检测过量表达和干扰circHIPK3对细胞增殖的影响; 。双荧光素酶报告基因实验分别检验miR-379与circHIPK3及miR-379与IGF1 mRNA的结合情况。Western; blot和ELISA检测细胞内外的IGF1蛋白表达水平。结果环状RNA; circHIPK3在6株NSCLC细胞株中均有表达,其中H1299表达最低,H2170表达最高,通过转染过表达的circHIPK3可显著促进NC; I-H1299细胞增殖,干扰circHIPK3可显著抑制NCI-H2170细胞增殖。miR-379可与circHIPK3及IGF1; mRNA直接结合。过表达circHIPK3导致IGF1表达量上调,干扰circHIPK3能够下调IGF1表达水平,转入miR-379; mimics恢复了circHIPK3稳转细胞株IGF1表达水平的上调及细胞增殖表型。结论环状RNA; circHIPK3在NSCLC细胞系NCI-H1299及NCI-H2170中可通过miR-379调控IGF1表达促进细胞增殖,环状RNA; circHIPK3可能成为非小细胞肺癌治疗的新靶点。Background and objective It has been proven that the circular RNA,; possessing a stable covalently closed continuous loop, is a type of RNA; molecule which is expressed widespread in mammals. The circular RNA; circHIPK3 is abundantly expressed in hepatocellular carcinoma (HCC) and; promotes tumourgenesis. However, a role for circHIPK3 has not been; systematically examined in non-small cell lung cancer (NSCLC). In this; study, we investigated whether circHIPK3 has an effect on cell; proliferation in the NSCLC cell lines NCI-H1299 and NCI-H2170 and the; underlying molecular mechanisms. Methods The expression of circHIPK3 was; measured by real-time PCR in NSCLC cell lines. Nuclear mass separation; experiment was used to detect the location of circHIPK3 in cells. The; gain and loss function experiments were used to examine the; proliferation of NCI-H1299 and NCI-H2170 cells by Cell Counting Kit-8; (CCK-8) and the colony formation assays. Then, circHIPK3 was cloned into; the downstream of the luciferase reporter gene which activity was; detected to verify whether miR-379 could bind with circHIPK3 or IGF1; mRNA. The protein level of IGF1 was detected by Western blot and ELISA; in circHIPK3 overexpressed/knock-down NCI-H1299 and NCI-H2170 cells.; Results CircHIPK3 was generally expressed in six kinds of NSCLC cells; lines we detected, and the expression level was highest in H2170 and; lowest in H1299. Overexpression of circHIPK3 obviously promoted; NCI-H1299 cell proliferation and knock-down of circHIPK3 inhibited; NCI-H2170 cell proliferation. In the luciferase assay, miR-379 was; observed to sequester circHIPK3 and IGF1 mRNA with potential binding; sites. Furthermore, we found that the overexpression of circHIPK3 could; increase the expression levels of IGF1 and knockdown reduced it.; Moreover, up-regulation of miR-379 rescued the phenotype induced by; overexpression of circHIPK3. Conclusion CircHIPK3 could promote cell; proliferation by a circHIPK3/miR-379 pathway in NCI-H1299 and NCI-H2170; cells and might be a potential tumor biomarker for NSCLC.国家自然科学基金项

总体性坍塌之后——论现代文学批评史写作模式流变

现代文学批评史写作始于20世纪80年代,这是正统文学史观自身的修复期和瓦解期。王永生主编的现代文学批评史表现出这样的基本特征,一方面要维护正统文学史观蕴含的历史总体性,另一方面试图修复它的学科性。作为新一代的学人,温儒敏悬置现代文学批评史的总体判断,而刘锋杰则坚持八十年代的启蒙主义和审美主义,他重申现代文学的未完成性,这是对总体性问题的重置。到了新世纪,在“复数现代性“的语境中,旷新年和余虹按照不同的路径重述现代文学理论史,旷以新左派的理论来激活左翼文学理论的反抗活力,而余虹则试图在新的知识条件下拯救出文学对历史发言的能力