Studies on male sterility of Brassica crops in terms of developmental biology

针对雄性不育性发生的发育生物学问题，从雄性不育发生的发育阶段调控和环境调控角度综述了芸薹属植物雄性不育发生的细胞学、生理生化以及分子生物学的研究进展，提出从DNA水平和基因表达水平以及应用细胞内信号传导模型探索雄性不育发生的分子机理的思路，并对其在分子育种中的应用作了展望。Concerning the terms of developmental biology of male sterility, the present reviewdescribed the major achievements in the study of developmental mechanism of Brassica crop malesterility involved in developmental stage regulation and environmental regulation. The authorseyaluated the molecular biological mechanism in DNA level, transcriptional level and signal trans-duction model, as well as their applications in molecular breeding.国家自然科学基金;;中国博士后科学基

Analysis of genetic polymorphisms in vegetable crops of Brassica campestris by RAPD markers

采用随机引物扩增多态性 DNA( RAPD)技术 ,对芸薹类 ( 2 n=2 0 )蔬菜作物的 3 3个品种进行了遗传多样性检测 .从 70个引物中筛选出 3 7个引物 ,共扩增出 3 53带 .其中 ,多态带有 2 60条 ,扩增片段长度大多数集中在 0 .9～ 1 .6kb之间 .不同引物的检测效率相差很大 .运用 5个引物扩增的 1 0条RAPD特征带 ,可以作为一组 DNA指纹 ,区分所有供试 7个大白菜品种WT5”BZ]The genetic polymorphisms in vegetable crops of Brassica campestris was investigated using random amplified polymorphic DNA markers(RAPDs), the detected materials included thirty three cultivars. Thirty seven primers selected from seventy primers amplified 353 RAPD fragments and 260 bands of them showed polymorphisms. The length of most amplified fragments ranged from 0.9 to 1.6 kb. Different primers exhibited different detective efficiency. There were ten RAPD characteristic markers , which were detected as a group of DNA fingerprints to identify all the seven cultivars of heading type Chinese cabbage. [WT5”HZ]浙江省自然科学基金!(395 0 6 4

Partial Cloning and Sequence Analysis of the Genic Male Sterile Gene in Brassica Campestris

通过 RAPD标记 ,所获得的与育性基因紧密连锁的基因片段进行克隆与序列分析 ,结果表明 ,该育性基因片段为与油菜小孢子发育早期 BP4调控基因 58%同源 ,并含有一个 MADS盒高度同源的保守序列 .Southern杂交结果表明该基因为单拷贝基因We have previously identified a specific RAPD marker associated with genic male sterile, out of 300 markers of the genomic DNA from both sterile and fertile plants. Analysis of this marker with genomic DNA from other 200 restores lines and sterile lines further proved that this marker was linked to genomic DNA. By using this RAPD marker amplified with the primer CTGCATCGTG at a specific site (about 720 bp) in bulkedDNA from maintaining lines, the linked gene was partially cloned and sequence analysed. Southern blotting showed that it is single copy gene, with 58% homologous to the Brassica napus pollenspecific gene Bp4, which is activated during early microspore development. It consisted of the promoter, the TATA signal for Bp4 gene and a MADS box. homologue sequences.国家自然科学基金资助项目!(9870461和9670 512);; 福建省自然科学基金重点资助项目!(B9920001

A PRELIMINARY RAPD ANALYSIS of THE GENETIC POLYMORPHISMS IN VEGETABLE CROPS of Brassica campestris (2n=20)

本文对芸薹种（ brASSICA CA M PESTrIS ，2n ＝ 20） 蔬菜基因组 d n A 进行了 r A P d 初步分析， 并就 r A P d- P C r 反应条件的优化进行了探讨。结果表明：各个亚种或变种的品种之间存在着丰富的遗传多样性。In this paper some studies on the genetic polymorphisms in vegetable crops of Brassica campestris (2n=20) by RAPD were conducted , meanwhile some discussions about the modification of RAPD-PCR were given.The study results indicated there were abundant genetic polymorphisms among the cultivars in different subspecies and varieties

蓄热式高温空气发生器冷态实验研究

介绍了基于换向式蓄热燃烧的高温空气发生工艺，并进行了冷态实验。结果表明调节鼓风机和排烟机的开度，可调节高温空气流量，分流出口处空气流量调节范围0－0.102m^3/s，分流出口处空气压力调节为0－210Pa

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel