利用改良Nest-tetra-primerspecificPCR技术对两种石斑鱼的鉴别

云纹石斑鱼(EPInEPHEluS MOArA)和褐石斑鱼(E.brunEuS)为石斑鱼属内近缘种,外形相似常被混淆.本研究改良建立了nEST-TETrA-PrIMEr SPECIfIC PCr方法,获得了云纹石斑鱼和褐石斑鱼线粒体dnAnd2基因内的3个特异性条带,分别为内参序列nC1(394bP)、特异性条带nd2-M(268bP)和nd2-b(122bP),以及核基因组中核糖体dnAITS1区的5个特异性条带,分别为内参序列nC2(588bP)、nC3(563bP),特异性条带rdnA-M(426bP)、ITS1-M(488bP)和ITS1-b(304bP).研究结果不仅为两种石斑鱼的鉴别提供了稳定、可靠、快捷的特异性分子标记,而且也为鱼类近缘种的dnA鉴别提供了新的途径.国家自然科学基金(批准号:40576064);国家“八六三”计划(项目编号:2006AA10A414)资助项

福建省自主创新政策工具的互补性研究

本文以福建省创新政策调查数据为基础,利用互补性的超模函数表述方式,从定量的角度实证检验福建省自主创新政策工具在不同阶段的互补性。结果显示,在提高企业创新力度阶段,人才资源方面的政策与资金投入、外部技术支持、科技创新基地及平台等三方面的政策均存在互补性关系,外部技术支持方面的政策和科技创新基地及平台方面的政策之间存在替代性关系;在创新成果转化阶段,知识产权保护方面的政策和成果转化与产业化方面的政策之间存在替代性关系。福建省软科学项目“国内外自主创新政策比较及海西经济区自主创新政策框架设计”(项目编号:2014R01010061

澄江针灸学派传人留章杰学术思想简析

留章杰先生是澄江针灸学派创始人承淡安先生的亲传弟子之一,福建针灸名家。收集整理留章杰先生的相关论文、医案及书籍,并访问其弟子张永树先生,分析总结其学术特点主要有:注重指力、运针不痛,擅用直灸、振阳祛邪,强调经脉辨证、四诊合参,凡病必有因、攻邪逐害为其要,重视疗效、不泥于法、针药并施。留章杰先生继承学派学术,并有进一步发挥,是澄江针灸学派代表性传人之一

海洋微生物与噬菌体间的相互关系

病毒是海洋中丰度最高的生物体,其中绝大多数又为能够侵染细菌和古菌的噬菌体.它们在控制微生物死亡率、调节微生物群落结构与多样性、影响微食物网过程以及参与碳、氮等元素的生物地球化学循环等方面扮演着重要的生态角色.本文对近年来关于海洋细菌与其病毒间相互关系的研究进行了概述,并结合作者的工作对未来的研究进行展望.国家自然科学基金(41006087;91028001;41076063);近海海洋环境科学国家重点实验室(厦门大学)青年访问学者基金(MELRS0932);福建省科技计划重点项目(2009Y0044)资

A Method for Assessing the Sustainability of Design in Developing World Projects

Projects for the developing world usually find themselves at the bottom of an engineer’s priority list. There is often very little engineering effort placed on creating new products for the poorest people in the world. This trend is beginning to change now as people begin to recognize the potential for these projects. Engineers are beginning to try and solve some of the direst issues in the developing world and many are having positive impacts. However, the conditions needed to support these projects can only be maintained in the short term. There is now a need for greater sustainability. Sustainability has a wide variety of definitions in both business and engineering. These concepts are analyzed and synthesized to develop a broad meaning of sustainability in the developing world. This primarily stems from the “triple bottom line” concept of economic, social, and environmental sustainability. Using this model and several international standards, this thesis develops a metric for guiding and evaluating the sustainability of engineering projects. The metric contains qualitative questions that investigate the sustainability of a project. It is used to assess several existing projects in order to determine flaws. Specifically, three projects seeking to deliver eyeglasses are analyzed for weaknesses to help define a new design approach for achieving better results. Using the metric as a guiding tool, teams designed two pieces of optometry equipment: one to cut lenses for eyeglasses and the other to diagnose refractive error, or prescription. These designs are created and prototyped in the developed and developing worlds in order to determine general feasibility. Although there is a recognized need for eventual design iterations, the whole project is evaluated using the developed metric and compared to the existing projects. Overall, the success demonstrates the improvements made to the long-term sustainability of the project resulting from the use of the sustainability metric

九孔鲍暴发性流行病的病原及病理

1999年 2～ 5月 ,东山县养殖九孔鲍暴发了大规模流行病 ,不少养殖场全场覆没 .病鲍表现为分泌粘液增多、肝脏红肿、足部僵硬和反应迟钝 .应用磷钨酸负染、超薄切片的电镜观察和现场检测等方法 ,对病鲍的病原及肝肠组织的病理情况进行观测 ,结果表明引发这次养殖鲍暴发严重病害的主要病原是致病力很强的病毒和弧菌 .电镜观察到病毒发生在细胞质中的一种称为“封入体”的泡状结构中 ,证实了病原的入侵造成九孔鲍肝及肠等组织、细胞产生病变 ,描述了细菌和病毒混合感染导致九孔鲍细胞的病理变

厦门地区无偿献血者HEV隐性感染情况及基因型分析

目的了解厦门地区无偿献血者中戊型肝炎的感染情况。方法对2005年3月—2007年4月厦门地区20389人(次)无偿献血者做整群抽样,捕获法ElISA检测IgM抗-HEV、并对IgM抗-HEV阳性样本做HEVrT-PCr检测,经测序后分析其基因型及序列同源性。结果20389份无偿献血者血样本中,IgM抗-HEV阳性率为0.91%(186/20389);186例IgM抗-HEV阳性中有4例经rT-PCr检测为HEV阳性。献血者中病毒血症总阳性率为2/10000(4/20389),4例病毒血症中Ⅰ型与Ⅳ型感染各2例。结论厦门地区无偿献血者中,存在HEV隐性感染者。厦门市卫生局医学科研项目资助课题(编号:WSK0516