不同因素对酶联免疫法测定破伤风免疫球蛋白活性的影响

目的考察不同因素对酶联免疫法（ELISA）测定破伤风免疫球蛋白生物活性的影响。方法将破伤风免疫球蛋白加入不同pH的缓冲液、不同浓度的氯化钠、硫酸铵以及聚乙二醇（PEG，相对分子质量为1000，6000和10000）溶液，采用ELISA法测定其活性。结果pH5～9的缓冲溶液对活性测定的影响不大，而pH≤4和≥10的缓冲溶液都会使测定值显著降低，当pH〈2或pH〉12时，活性下降到正常值的10％以下。随氯化钠浓度的增加测定值先下降到正常值的37．3％，后回升到41．2％，再下降；随硫酸铵浓度的增加测定值先下降到正常值的67％，后回升到99．4％，再下降。PEG对活性测定有较大影响，随PEG浓度的增加活性测定值降低。结论缓冲液pH、盐以及PEG对破伤风免疫球蛋白活性测定有影响

不同因素对酶联免疫法测定破伤风免疫球蛋白活性的影响

目的考察不同因素对酶联免疫法(ELISA)测定破伤风免疫球蛋白生物活性的影响。方法将破伤风免疫球蛋白加入不同pH的缓冲液、不同浓度的氯化钠、硫酸铵以及聚乙二醇(PEG,相对分子质量为1 000,6 000和10 000)溶液,采用ELISA法测定其活性。结果pH 5~9的缓冲溶液对活性测定的影响不大,而pH≤4和≥10的缓冲溶液都会使测定值显著降低,当pH<2或pH>12时,活性下降到正常值的10%以下。随氯化钠浓度的增加测定值先下降到正常值的37.3%,后回升到41.2%,再下降;随硫酸铵浓度的增加测定值先下降到正常值的67%,后回升到99.4%,再下降。PEG对活性测定有较大影响,随PEG浓度的增加活性测定值降低。结论缓冲液pH、盐以及PEG对破伤风免疫球蛋白活性测定有影响

JUNO Sensitivity on Proton Decay p→νˉK+p\to \bar\nu K^+ Searches

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this paper, the potential on searching for proton decay in $p\to \bar\nu K^+$ mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits to suppress the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar\nu K^+$ is 36.9% with a background level of 0.2 events after 10 years of data taking. The estimated sensitivity based on 200 kton-years exposure is $9.6 \times 10^{33}$ years, competitive with the current best limits on the proton lifetime in this channel

JUNO sensitivity on proton decay p → ν K + searches*

The Jiangmen Underground Neutrino Observatory (JUNO) is a large liquid scintillator detector designed to explore many topics in fundamental physics. In this study, the potential of searching for proton decay in the $p\to \bar{\nu} K^+$ mode with JUNO is investigated. The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification. Moreover, the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals. Based on these advantages, the detection efficiency for the proton decay via $p\to \bar{\nu} K^+$ is 36.9% ± 4.9% with a background level of $0.2\pm 0.05({\rm syst})\pm$$0.2({\rm stat})$ events after 10 years of data collection. The estimated sensitivity based on 200 kton-years of exposure is $9.6 \times 10^{33}$ years, which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies