Promotion Strategy of O2O Platform for Retail Industry——A case based on Feifan APP

摘要 我国的实体百货行业在经历了快速发展和电子商务浪潮的冲击后，进入了低谷期，传统百货的商业模式亟待创新。大量传统零售百货企业引入了O2O模式，搭建了网络平台用于辅助或加强传统销售。对比线上、线下零售产业的现状和困境，引入实体零售百货O2O的商业模式，及实体零售百货O2O平台。平台针对整个零售行业，力求整合商业生态圈资源，为用户提供更多的资讯，为线下商业带来更多的客流，让线上线下互动起来。 以系统性的创新思维方式，运用文献分析法和归纳演绎法法分析实体零售百货O2O平台及平台的需求分析和平台的商务模式分析。再通过案例分析法对腾讯、百度、万达联合开发的飞凡电商平台进行案例研究。采用数据分析的方...Abstract After a period of rapid development and great challenges from e-commerce, retail industry in our country has fallen into the trough. The traditional business mode in this industry should be innovated immedately. A large number of traditional retail enterprises introduced the O2O model and built network platform to assist or strengthen their traditional sales. Comparison of online and off...学位：管理学硕士院系专业：管理学院_工商管理硕士(工商管理硕士)学号：1792015115174

An Empirical Study on the Importance of English Phonological Competence of College Students

语言是人类交流的工具,特别是有声语言通过声音来传达信息，因此,发声的准确性就起了很重要的作用。直到今天，语音教学的研究逐渐引起人们的关注。然而，语音能力在英语教学中经常被认为无足轻重。从学生的角度来说,在英语学习过程中遇到了更多的困难和产生更多的错误,特别是在语音方面。较弱的语音能力阻碍了学生其它语言技能的发展,如词汇、语法和阅读。 这篇论文意图从三个方面来阐述这些问题：1）语音能力对于相关语言能力如语法、词汇、阅读的影响;2)目前语音教学存在的问题;3)分析原因及提出解决方法。 因此，用两种方法来证明这些问题:1)测试语音（附录I&II）、语法（附录III）、词汇和阅读能力（附录...Language is the tool for human communication, in particular, vocal language expresses information through sounds. So the accuracy of the sounds plays an important role. To this day, the interest in the research on phonological teaching and learning has gradually drawn people's attention. However, phonological competence often seems to be deemed as something that little attention is given to in Eng...学位：文学硕士院系专业：外文学院英语语言文学系_英语语言文学学号：X200511003

Quantification of Methylation at CMV Promoter of pEGFP-C3 Vector by Gradient Touch-Down PCR Sequencing

目的改进亚硫酸氢钠测序法,并在CMV基因启动子甲基化检测中进行验证。方法提取PEgfP-C3质粒重组人肝癌细胞株HEPg2 dnA,亚硫酸氢钠化学修饰,针对修饰后质粒基因CMV启动子序列设计特异引物并结合梯度降落PCr扩增,T-A载体克隆、测序,目标区域甲基化定量。结果 PEgfP-C3质粒基因约600bP的CMV启动子区甲基化水平可以精确定量,检测结果与标准品一致,重复测量结果稳定。结论改进后亚硫酸氢钠测序法能明显减少非特异性扩增,提高PCr效率,更适于基因甲基化状态的检测。Objective To improve the sodium bisulfite sequencing method and validate it in quantification of methylation in CMV promoter.Methods DNA was extracted from recombinant HepG2 hepatoma cell line with plasmid pEGFP-C3,and chemically modified with sodium bisulfite,the gene-specific primers were designed according to the modified CMV promoter sequence and conducted PCR amplification with gradient touch-down PCR,then the DNA methylation level in the target areas was quantitated after T-A cloning and sequencing.Results The DNA methylation level of the 600 bp CMV promoter within pEGFPC3 plasmid could be quantified accurately,and be consistent with that of the methylated DNA standards,repeated measurements indicated stable quantification result with this method.Conclusion The improved sodium bisulfite sequencing method can reduce non-specific amplification significantly and improve PCR efficiency,therefore has more potential for quantitative detection of gene methylation status.国家自然科学基金资助项目(20907047);中央级公益性科研院所基本科研业务专项(2008KYYW05

从中英文的部分差异浅谈英语学习

本文将比较中英文的词分类、语态、语气、时态等方面的差别,并且提出关于英语学习的一些建议,从而使英语教与学的质量及效率得到提高

Inducing method and application of shellfish blood cell extracellular trap

本发明涉及一种贝类血细胞胞外陷阱的诱导方法。采用酵母多糖对贝类血细胞进行诱导，获得发生胞外陷阱的贝类血细胞。有益效果：将蛤仔血细胞用酵母多糖诱导，使得血细胞胞外陷阱的发生。本发明的方法能够有效诱导蛤仔血细胞发生胞外陷阱，并抑制细菌生长

胞外陷阱的免疫学研究现状及展望

Extracellular traps (ETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. Presently, most of the research on ETs was focused on the study of their inducing factors and antibacterial activities. However, the study on the formation mechanism of ETs, and the function of mitochondria in releasing ETs are still in its infancy. To gain insight into the study, function and action mode were performed to characterize the ETs formation mechanism and elucidate the changes of mitochondria preliminarily. Additionally, ETs could be degraded by DNA enzyme. Those above provides helpful evidence for revealing the mechanism of ETs formation, action mode and elimination, deepening the cognition on the innate immune defense in invertebrates, as well as extending the immunology boundary of marine invertebrates.</p

A Peptidoglycan Recognition Protein (PGRP) from Haliotis discus hannai: Possible Roles in Antibacterial Properties

In this study, peptide PGRP (designated HdPGRP) was identified and characterized from the abalone Haliotis discus hannai. Multiple alignments and phylogenetic analyses strongly suggested that HdPGRP is a new member of the PGRP superfamily and belongs to the short PGRP family, similar to peptides from other marine mollusks. The full length of HdPGRP is 1467 bp, encoding a polypeptide of 354 amino acids (aa) with a signal peptide (1~18 aa), an SH3b domain (93~160 aa), a typical PGRP domain (179~322 aa), and an Ami_2 domain (191~322 aa). In addition, four conserved Zn~(2+)-binding sites (H~(209), Y~(255), H~(318), and C~(330)) and five conserved amide-catalysis sites (H~(209), Y~(255), H~(318), T328, and C~(330)) were found in the HdPGRP sequence. In abalone, hdpgrp exhibited different tissue expression patterns, and was strongly expressed in the hepatopancreas, moderately expressed in hemocytes, mantle, and gills, and slightly expressed in muscle. Vibrio anguillarum is one of the main pathogens of H. discus hannai; after V. anguillarum infection, expression of hdpgrp in hemocytes showed a trend of first increasing and then decreasing, reaching a maximum at 24 h. Subsequently, expression of HdPGRP decreased, and there was no significant difference compared with the control group at 72 h, demonstrating that expression of HdPGRP had returned to normal levels. SDS-PAGE results showed that recombinant HdPGRP (rHdPGRP) has a molecular mass of 30 kDa, which is in line with the value predicted for HdPGRP. PGRPs usually have amidase activity, degrading peptidoglycan by hydrolyzing the amide bond that links peptide units to muramic acid residues of glycan strands. rHdPGRP exhibited Zn~(2+)-dependent amidase activity and catalyzed the degradation of insoluble peptidoglycan. In addition, rHdPGRP exhibited significant antibacterial activity against the gram-positive bacterium Micrococcus luteus in the logarithmic phase in the presence of Zn~(2+), indicating that the antibacterial activity of HdPGRP might be dependent on amidase activity. In summary, HdPGRP plays an important role in PGRP-mediated antibacterial mechanisms, especially for eliminating invading bacteria

Auto-feedingTechnique Of AFM Probes Based On Machine Vision

原子力显微镜(AFM)是微纳米操作中不可或缺的有效工具，在材料、生物等领域的研 究中发挥着重要作用。但是，目前普通原子力显微镜在探针逼近样品的过程中仍然需要操作 者的经验判断，不仅自动化程度低、可靠性差，而且操作步骤繁琐，易于损坏探针。为了解 决上述问题，本文提出一种基于机器视觉和精确力反馈控制的AFM 探针自动逼近方法。该方 法分为粗、精两步完成探针快速逼近。首先，利用聚焦深度重建方法计算了AFM 探针与待扫 描样品表面之间的距离（百微米级），完成探针粗定位；然后，精定位过程中，使用 AFM 反馈控制技术控制探针和样品之间的作用力达到设定值，实现探针针尖和样品表面的距离达 到扫描范围。实验表明，该方法定位精度高，稳定可靠，对探针无损害，可实现探针和样品 的连续自动逼近，对于提升AFM 的操作效率具有重要意义

Auto-feedingTechnique Of AFM Probes Based On Machine Vision

原子力显微镜(AFM)是微纳米操作中不可或缺的有效工具，在材料、生物等领域的研 究中发挥着重要作用。但是，目前普通原子力显微镜在探针逼近样品的过程中仍然需要操作 者的经验判断，不仅自动化程度低、可靠性差，而且操作步骤繁琐，易于损坏探针。为了解 决上述问题，本文提出一种基于机器视觉和精确力反馈控制的AFM 探针自动逼近方法。该方 法分为粗、精两步完成探针快速逼近。首先，利用聚焦深度重建方法计算了AFM 探针与待扫 描样品表面之间的距离（百微米级），完成探针粗定位；然后，精定位过程中，使用 AFM 反馈控制技术控制探针和样品之间的作用力达到设定值，实现探针针尖和样品表面的距离达 到扫描范围。实验表明，该方法定位精度高，稳定可靠，对探针无损害，可实现探针和样品 的连续自动逼近，对于提升AFM 的操作效率具有重要意义