公共场所监测数据处理及场所质量卫生综合评价

对公共场所监测数据和场所质量卫生综合评价作出了论述,以期为开展具体工作提供指导

福建省农村饮用水水源水质卫生学研究

为了解福建省农村饮用水水源水质卫生状况,以保障人体健康及提高我省农村饮用水水源水质卫生质量及水源卫生防护提供科学依据。我们对福建省29个有代表性的县饮用水水源及其水质按国家GB5750-85《生活饮用水标准检验法》进行了检测,根据国家《农村实施(生活饮用水卫生标准)准则》进行评价

Medium Optimization of Astaxanthin by Oceanic Red Yeast

为了提高海洋红酵母发酵虾青素的产量水平,对海洋红酵母的培养基成分进行了初步研究。试验结果表明,海洋红酵母能利用葡萄糖、淀粉水解糖、糖蜜等多种碳源,用淀粉水解糖为碳源培养海洋红酵母所获得的虾青素体积产率最大;用牛肉膏为氮源有利于提高海洋红酵母的生物量,以(NH4)2SO4、NH4Cl和蛋白胨为氮源有利于提高海洋红酵母的虾青素体积产率,用KNO3、草酸铵、蛋白胨、尿素有利于提高海洋红酵母的虾青素细胞产率;在海洋红酵母的培养基中添加Mn2+、Cd2+、Zn2+、Fe2+能增加生物量,添加Zn2+、Fe3+、Mn2+能增加海洋红酵母的虾青素体积产率,添加Fe3+能提高海洋红酵母的虾青素细胞产率。In order to improve the production level of astaxanthin by oceanic red yeast(ORY),the ingredients of medium for it were studied.The results of experiment showed that ORY could utilize many kinds of carbon sources such as glucose,starch hydrolyzed sugar(SHS),and molasses etc.However,volume productivity of astaxanthin was the biggest among these sugars when using SHS.Using beef extract as nitrogen source is helpful to enhance biomass of ORY, while(NH4)2SO4,NH4Cl and peptone were conducive to improve the ORY volume productivity of astaxanthin.In addition,KNO3,ammonium oxalate,peptone,and urea were conducive to improve the cellular productivity of astaxanthin.Adding Mn2+,Cd2+,Zn2+,and Fe2+ to the ORY medium,could increase the biomass.Adding Zn2+,Fe2+,and Mn2+ could increase the ORY volume productivity of astaxanthin.And adding Fe2+ could increase the ORY cellular productivity of the astaxanthin.福建省重点科技项目(2006H0033);; 厦门市科技项目(B04066

船舶压舱水引入外来藻类的危害及监测

船舶压舱水是外来海洋生物入侵的主要媒介之一,是造成有害赤潮藻类世界范围的传播蔓延的重要原因。有害藻类的引入对当地的生态系统和经济已造成严重和深远的影响,是植物检疫的盲区。本文论述了压舱水引入外来藻类尤其是有害藻造成危害的问题和检疫技术。以引起有关部门的重视,解决压舱水藻类检测和检疫处理问题

The Effects of Different Seawater Concentration and Culture Time on Antimicrobial Activity of Marine Fungi

作者简介: 杜希萍女,讲师。现从事天然产物化学方面的研究。E-mail: xipingdu@ jmu.edu.cn 通讯作者。E-mail: wjsu@ jmu.edu.cn[中文文摘]用滤纸片琼脂扩散法研究不同海水浓度和培养时间对海洋真菌抗菌活性的影响,旨在为大规模培养海洋真菌和提高获得抗菌物质的几率提供理论依据。结果显示:用不同海水浓度培养基发酵海洋真菌,在供试的10株海洋真菌中,5株海洋真菌的抗菌活性和抗菌谱有明显差异;培养时间不同,6株海洋真菌的抗菌活性差别较大。实验结果表明,不同海水浓度和培养时间对海洋真菌抗菌活性有显著影响。[英文文摘]The effects of different seawater concentration and culture time on antimicrobial activity of marine fungi were studied using agar diffusion method with filter paper diskettes,aiming at providing theoretical foundation for culturing marine fungi on a large scale and improving the probability of antimicrobial metabolites.The results showed that the differences of antimicrobial activities and antimicrobial spectrum of five among ten tested marine fungal strains cultured in different seawater concentration media were fairly obvious; antimicrobial activities of six marine fungal strains were significantly different at the different culture time.The study indicated different seawater concentration and culture time greatly affected the antimicrobial activity of marine fungi．集美大学博士启动基金(ZQ2011004);中央高校基本业务费(2010121092)资

公共浴室预防性卫生审查项目与要求

结合历年的监测实践,提出公共浴室预防性卫生审查项目和要求,以满足卫生监督执法需要

文化娱乐场所预防性卫生审查内容和要点

结合历年的卫生监测实践,提出文化娱乐场所预防性卫生审查的内容和要点,以满足卫生监督执法、监测发证实际工作需要,对开展具体工作具有实际指导意义

heuristic symbolic verification of safety properties for parameterized systems

参数化系统(paramterized system)是指包含特定有限状态进程多个实例的并发系统,其中的参数是指系统内进程实例的数目,即系统的规模.反向可达性分析(backward reachability analysis)已被广泛用于验证参数化系统是否满足以向上封闭(upward-closed)集合表示的安全性(safety property).与有限状态系统验证相类似,参数化系统的验证同样也面临着状态爆炸(state explosion)问题,并且模型检测算法的有效性依赖于如何采用有效的数据结构表示状态集合.针对表示无穷状态的向上封闭集合,研究人员提出了多种基于约束(constraint-based)的符号表示方法.但这些方法依然面临着符号状态爆炸(symbolic state explosion)问题或者其包含判定问题,即判断一个约束条件集合符号化表示的实际状态集合是否为另一约束条件集合所对应的状态集合的子集,是Co-NP完全问题.因此,虽然有限状态验证技术能够验证一些具有一定规模的问题,但现有针对参数化系统的验证方法所能解决的问题的规模较为有限,需要近一步提高模型检测算法的效率.针对参数化系统提出了加快反向可达性分析的多个启发式规则,实验结果表明,这些启发式规则可以使算法的效率提高几个数量级,从而有助于解决现有参数化系统验证方法所存在的问题

福建省农村饮用水水质调查分析

为改善、提高福建省农村饮用水水质卫生质量及水源卫生防护提供科学依据。[方法]对福建省14个代表性县饮用水水源及其水质按国家GB5750-85《生活饮用水标准检验法》进行了检测,根据国家《农村实施(生活饮用水卫生标准)准则》进行评价。[结果]12年共检测551份水样,其中丰、枯水期分别检测293和258份,丰水期总大肠菌群和细菌总数超标率分别为63%和63%;枯水期总大肠菌群和细菌总数超标率分别为61%和50%。[结论]总大肠菌群、细菌总数、浑浊度、色度、铁、锰、氟化物均不同程度严重超标

Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide

提出并发展了一种基于电感耦合等离子体质谱(ICP-MS)的双元素标签标记策略来选择性识别和检测硒蛋白/多肽,其中内源元素硒(SE)作为硒蛋白/多肽分子的识别元素,外源元素汞(Hg)作为硒蛋白/多肽和含硒蛋白/多肽分子的区分元素。通过对硒代半胱氨酸(SECyS)和谷胱甘肽过氧化酶1(gPX1)两种模型分子的研究,外源邻羧基苯硫甲基汞(CH3Hg-THI)动态解离的CH3Hg+能够选择性标记硒代半胱氨酸残基(SECyS)中硒醇基(-SE H),但不能标记含硒蛋白/多肽分子的硒代蛋氨酸残基(SE MET)中的—SECH3,进而依据SE和Hg的ICP-MS信号识别和检测硒蛋白/多肽。本方法应用于富硒酵母水溶性提取液的分析,结果表明,提取液中的硒蛋白/多肽能够被有效识别和检测,验证了SE-Hg双元素标签标记策略的发展是ICP-MS识别和检测硒蛋白/多肽的一种可行且优越的途径。An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma mass spectrometry( ICP-MS) to screen and discriminate a family of ultratrace but biological important biomolecules.The feasibility of this novel idea has been demonstrated when setting seleno( Se Cys)and Se-containing( Se Met) proteins( peptides) as an example.Se-label naturally occurring in the biomolecules acts an identifier for picking them up out of large amount of various coexisting proteins( peptides),and CH3Hg-tag that can bind to Se Cys but not Se Met fulfills the task of discriminating seleno and Se-containing ones based on the Se and Hg signals on ICP-MS.After confirmed using Se Cys and GPx1,the Se-Hg dualelement labeling strategy together with ICP-MS was applied to screen and discriminate seleno and Se-containing proteins( peptides) in the water-soluble extracts of Se-enriched yeast,and seven selenoproteins( peptides) were detected with both202 Hg and82Se signals out of fifteen Se-containing species using RPLC / ICPMS,providing valuable information for further identification using a high-resolution structure-selective mass spectrometer.This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins( peptides) but also helpful to recognize targeted proteins during a discovery-based proteomic study.国家自然科学基金资助项目(Nos.2135006;21275120;21475108;21407169)~