Airborne fine particle decreases the cell viability and induces inflammation in human bronchial epithelial cells

目的:研究大气细颗粒物(fine particulate; matter,PM_(2.5))对人支气管上皮细胞活性的影响及其炎性作用。方法:用PM_(2.5)采样器采集上海地区大气PM_(2.5)样本,扫; 描电镜观察PM_(2.5)形态特征。将人支气管上皮细胞BEAS-2B暴露于不同浓度(0,50,100,200,400,800; mug/mL)的PM_(2.5) 12,24,48 h,细胞活力检测试剂盒(cell counting kit-8,; CCK-8)法检测PM_(2.5)暴露对细胞活性的影响。实时定量PCR(quantitative real-time; PCR,qRT-PCR)检测细胞粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating; factor,GM-CSF)和TNF-alpha mRNA的表达,Western印迹检测; GM-CSF和TNF-alpha蛋白的表达。结果:扫描电镜观察发现,PM_(2.5)形态多样,大小不一,直径大多等于或小于2.5; mum。与同时间点未暴露组比较,各暴露组(50~800; mug/mL)细胞活性呈不同程度的下降,差异具有统计学意义(P<0.05)。与未暴露组比较,暴露于100,400或800 mug/mL; PM_(2.5) 24 h后,GM-CSF和TNF-alpha; mRNA和蛋白表达水平明显升高(P<0.05),且PM_(2.5)暴露浓度越高,GM-CSF和TNF-alpha的mRNA和蛋白升高水平越显著。; 结论:大气PM_(2.5)可引起人支气管上皮细胞的炎症反应,降低细胞活性,这可能与PM_(2.5)促发和加重支气管肺部炎性疾病有关。Objective: To investigate the effects of airborne fine particle on cell; viability and inflammation in human bronchial epithelial cells. Methods:; Atmospheric PM_(2.5) samples were collected by PM_(2.5) sampler.; PM_(2.5) morphology was observed by scanning electron microscope (SEM).; Human bronchial epithelial cells (BEAS- 2B) were treated with PM_(2.5); at different concentrations (0, 50, 100, 200, 400, 800 mug/mL) for 12,; 24 or 48 hours, and the cell activity were evaluated by cell counting; kit-8 (CCK-8). The mRNA expression levels of (granulocyte-macrophage; colony stimulating factor,GM-CSF) and TNF-alpha were detected by; quantitative real-time PCR (qRT-PCR). Western blot was used to detect; the protein expressions of GM-CSF and TNF-alpha. Results: According to; SEM, the shape of PM_(2.5) varied, and the diameter was different and; mostly equal to or less than 2.5 mum. CCK-8 assay showed that different; concentrations of PM_(2.5) exposure for 12 hours, 24 hours and 48 hours; resulted in loss of cell viability of BEAS-2B cells (P<0.05). Different; concentrations of PM_(2.5) increased the mRNA and protein expression of; GM-CSF and TNF-alpha, and the higher concentration of PM_(2.5) induced; higher expression, which have statistical significant difference between; the groups (P<0.05). Conclusion: Atmospheric PM_(2.5) can cause; inflammatory response in human bronchial epithelial cells. They can; reduce cell viability, which may be related to the PM_(2.5) trigger and; aggravation of bronchopulmonary inflammatory diseases

Synthesis and Crystal Structure of Erbium Sesquiselenide Er_2Se_3

采用高温固相反应和化学气相传输法合成三硒化二铒。晶体属于四方晶系，M_r＝571.40，空间群为P4/nMM，A＝3.984（1），C＝8.228（3），V＝130.59（7），z＝1，d_C＝7，27g/CM￣3，f（000）＝238，μ（MOkα）＝528.86CM￣（-1），对于187个I≥3σ（I）的独立衍射点，最后偏离因子r＝0.066，r_W＝0.078。化合物Er_2SE_3的晶体结构是由Er配位多面体的双层状结构通过共用SE（2）原子，沿C轴方向堆积而形成的三维结构。Erbium Sesquiselenide Er_2Se_3 was prepared by solid state reaction.It crystallizes in the tetragonal space group P4/nmm with a= 3.984(1) , c=8.228(3) ,V=130.59(7), Z=1 ,D_c= 7.27 g/cm￣3 , F(000) =238,P(MoKα) = 528.86 cm￣(-1) , the Final R=0.066 , R_w = 0.078For 187 independent reFlections with I≥3σ(I).The structure of Er_2Se_3 is built From stacking the double layers of Er polyhedron through the Se(2) atoms along the c direction.国家自然科学基金;结构化学国家重点实验室资助;福建省自然科学基

三核钼－硫簇合物的低热固相合成及其晶体结构

三核钼－硫配合物Ｍｏ_３Ｓ_７（ｄｔｃ）_３Ｉ·Ｓ_８·２ＣＨ_２Ｃｌ_２（ｄｔｃ＝Ｃ_４Ｈ_８ＮＣＳ_２~－）是由低热固相合成得到的。晶体属单斜晶系，Ｍ_ｒ＝１５００．１９，空间群Ｐ２_１／ｎ，ａ＝１１．８８１（３），ｂ＝１５．５５９（４），ｃ＝２６．１９７（７），β＝９８．５３（２）°，Ｖ＝４７８９Ａ~３，Ｚ＝４，Ｄ_ｃ＝２．０８ｇ／ｃｍ~３，Ｆ（０００）＝２９２０，μ（ＭｏＫα）＝２５．２４ｃｍ~（－１），对于２３９７个Ｉ≥３σ（Ｉ）的独立衍射点，最终偏离因子Ｒ＝０．０６０，Ｒ＝０．０６６。该配合物的基本骨架是［Ｍｏ_３Ｓ_７（ｄｔｃ）_３］~＋，其簇芯为［Ｍｏ_３Ｓ_７］~（４＋）单元，３中Ｍｏ原子组成正三角形平面，Ｍｏ－Ｍｏ的平均键长力２．７２３，Ｍｏ原子平面上的硫原子形成盖帽的μ_３－Ｓ，其Ｍｏ－Ｓ平均键长为２．３８３Ａ，３个Ｓ_２基团分别位于三角形３个棱的外侧，并分别与邻近的两个Ｍｏ原子结合形成１２中Ｍｏ－Ｓ键。与μ_３－Ｓ相对而位于平面另一侧的Ⅰ原子与３个μ_２－Ｓ连结，平均距离为３．２５７Ａ，成键作用较弱