Studies on improved integrated membrane-based chromatographic process for bioseparation

To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process. Interfacial polymerization was carried out to coat the bottom section of the hollow fiber membrane while leaving the rest of the hollow fiber membrane unaffected. Myoglobin (Mb), bovine serum albumin (BSA) and α-lactalbumin (α-LA) were used as model proteins in binary mixtures. Separation behaviors of binary protein mixtures were studied in devices using either an ultrafiltration (UF) membrane or a microfiltration (MF) membrane. Experimental results show that the breakthrough time and the protein loading capacities were dramatically improved after coating in both UF and MF modules. For a synthetic yeast fermentation broth feed, the Mb and u-LA elution profiles for the four consecutive cyclic runs were almost superimposable. Due to the lower transmembrane flux in this device plus the periodical washing-elution during the chromatographic separation, fouling was not a problem as it is in conventional microfiltration. A mathematical model describing the hydrodynamic and protein loading behaviors of the integrated device using UF membrane with a coated zone was developed. The simulation results for the breakthrough agree well with the experimental breakthrough curves. The optimal length of the coated zone was obtained from the simulation. A theoretical analysis of the protein mass transfer was performed using a diffusion-convection model considering the feed-side concentration polarization and the permeate-side concentration gradient formed by the adsorption. The permeate-side adsorption can enhance the observed protein transmission through the membrane considerably at low permeate flux. But the enhancement effect can be neglected at higher permeate flux when convection dominates the total mass transfer process or the proteins are very highly rejected by the membrane

Media Specialists and Gaming

The purpose of this portfolio is to provide three games that a media specialist can use to help bring play to education and to enhance a student’s education. The media center should be an ever adapting environment and a place to provide students and teachers with new materials and ideas that fit their needs. Media specialists are in an excellent position to introduce digital games and collaborate with other educators and while doing this add more play to a testing and standard focused curriculum. The research in this portfolio will look to see how play and games are affecting education and what types of games are being used in the Media Center and in classrooms. This information will be used to inform the creation of three games a media specialist can use to enhance a child’s experience and education in the school. The first game, developed by the author, will provide students with fun and educational materials that can be used in and outside the classroom and help teachers differentiate instruction for their students. The second game, also developed by the author, will motivate and encourage students to read for enjoyment. The third project provides materials to integrate an existing game that teachers can use in any subject’s curriculum as a form of formative assessment and to help students work on the 4Cs

Modification of Bushing Test Rig and Research of Variable Inlet Guide Vane Bushings For Further Development of PM304 Bushings

PS304 is a high temperature solid lubricant coating comprised of a nickel-chrome binder, chrome oxide hardener, barium-calcium fluoride high temperature lubricant, and silver as the low temperature lubricant. This coating is used to lubricate Oil-Free Foil Air Bearings as they experience friction and wear during start up and shut down. The coating deposition process begins with undercutting the shaft. This area is then sandblasted to provide a rough surface for the coating to adhere to. The coating powder is then sent through the plasma spray gun and a reasonably thick layer is applied to the undercut area of the shaft. The coating is then ground down even with the surface of the shaft and gets a nice polished finish. The foil air bearings use the solid lubricant, as mentioned above, during start up and shut down. During normal operating conditions, generally above 2000RPM, the bearings utilize air as their lubricant. Foil air bearings are comprised of a thin top foil and a corrugated bump foil. They have an interference fit with the shaft before operation. As the air gets "trapped" between the top foil and the shaft, it presses the top foil against the bump foil, in turn compressing the bumps. As the bumps compress, it allows for the air to separate the top foil from the shaft, therefore, utilizing the trapped air as its lubricant

Asteroseismology of KIC 8263801:Is it a member of NGC 6866 and a red clump star?

We present an asteroseismic analysis of the Kepler light curve of KIC 8263801, a red-giant star in the open cluster NGC 6866 that has previously been reported to be a helium-burning red-clump star. We extracted the frequencies of the radial and quadrupole modes from its frequency power spectrum and determined its properties using a grid of evolutionary models constructed with MESA. The oscillation frequencies were calculated using the GYRE code and the surface term was corrected using the Ball & Gizon(2014) prescription. We find that the star has a mass of $M/M_{\odot} = 1.793\pm 0.072$, age $t=1.48\pm 0.21$ Gyr and radius $R/R_{\odot} = 10.53\pm 0.28$. By analyzing the internal structure of the best-fitting model, we infer the evolutionary status of the star KIC 8263801 as being on the ascending part of the red giant branch, and not on the red clump. This result is verified using a purely asteroseismic diagnostic, the $\epsilon_{c}-\Delta\nu_{c}$ diagram which can distinguish red giant branch stars from red clump stars. Finally, by comparing its age with NGC 6866 ($t=0.65 \pm 0.1$ Gyr) we conclude that KIC 8263801 is not a member of this open cluster

Nature of Science and Minnesota Chemistry Education

Minnesota chemistry teachers were surveyed to determine their practices for including nature of science content into general chemistry curriculum. Teacher experience, engagement in professional development and licensing institutions were compared with their tendency to include nature of science topics as part of their curriculum. Engagement in professional development was found to have the strongest relationship with a weak positive correlation. Participants also reported the specific nature of science standards they include in the instruction of general chemistry and the frequency of these responses are analyzed

New synchronization criteria for an array of neural networks with hybrid coupling and time-varying delays

This paper is concerned with the global exponential synchronization for an array of hybrid coupled neural networks with time-varying leakage delay, discrete and distributed delays. Applying a novel Lyapunov functional and the property of outer coupling matrices of the neural networks, sufficient conditions are obtained for the global exponential synchronization of the system. The derived synchronization criteria are closely related with the time-varying delays and the coupling structure of the networks. The maximal allowable upper bounds of the time-varying delays can be obtained guaranteeing the global synchronization for the neural networks. The method we adopt in this paper is different from the commonly used linear matrix inequality (LMI) technique, and our synchronization conditions are new, which are easy to check in comparison with the previously reported LMI-based ones. Some examples are given to show the effectiveness of the obtained theoretical results