1,290 research outputs found

    Sustainable deathstyles? The geography of green burials in Britain

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    In the context of a wider literature on ‘deathscapes’, we map the emergence of a new mode of burial and remembrance in Britain. Since a ‘green’ burial ground was established in Carlisle in 1993, sites for so-called ‘green, ‘natural’ or ‘woodland’ funerals have proliferated. There are now over 270 such sites in Britain. Drawing on a postal and email survey sent to all managers/owners and visits to 15 green burial grounds (enabling observations and semi-structured interviews with their managers), we chart their growth, establishment and regulation and describe the landscapes associated with them. This requires, and leads to, wider reflections on nature, capital, consumption, culture and the body

    The contribution of experts in care proceedings : evaluation of independent social work reports on care proceedings

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    Several concerns surround the use of independent social work (ISW) assessments in care proceedings. Some result from the exclusion of this work from a review of legal aid for expert assessments in this field, a cap on ISW fees, and fears about a resulting reduction in the availability of ISWs. At the same time, submissions to the Family Justice Review (FJR) claimed that ISWs cause delay, simply duplicate existing local authority assessments, add nothing new and undermine confidence in social work assessments. It was also said that ISW reports result from parents utilising human rights claims to gain a second opinion of a local authority assessment – and to which courts too readily accede. ... But while strong views have been expressed about the use of independent social work assessments, there is little hard evidence. This evaluation, commissioned following submissions to the FJR, begins to address that lack. It is based on 65 cases concerning 121 children and 82 reports for courts in England and Wales. The sample was drawn from the records of three independent agencies providing ISWs

    Outdoor recreation and nature conservation: conflicts and their resolution

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    Keeping a Happy Face: Managing Emotions in Teen Jobs

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    In Canada, many teen women hold jobs, particularly in the service sector, yet we know very little about their experiences as workers. This paper examines teen women's understandings of emotional labour as experienced within their frontline service jobs. We consider the broader significance of these findings in light of current literature on emotional labour and gender.Au Canada, un bon nombre d'adolescentes ont un emploi, tout particulierement dans le secteur de service, cependant nous savons tres peu au sujet de leur experiences en tant que travailleuses. Le present article etudie ce que les adolescentes comprennent du travail emotionnel tel que vecu dans leur travail de premiere ligne. Nous considerons la signification de ces decouvertes compte tenu de la litterature courante sur le travail emotionnel et la difference entre les sexes

    Geography’s Strategies

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    In the context of debates about policy relevance, geotechnologies, and the status of and prospects for geography, we present the case for a promotional strategy based on foregrounding the impact, diversity, and wealth of geographic scholarshi

    Duty, Passion and the (Re)Production of a Gendered Life: One Young Woman's Struggle to Craft a Meaningful Vocational Project

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    This longitudinal narrative case study traces one young woman's vocational journey from age 17 to 23. Drawing on youth transitions literature and feminist scholarship on the gendered division of labour, the paper considers how her journey was shaped by powerful ideologies linked to relational work, home-based production, and postsecondary schooling. Résumé Ce cas d'étude narratif longitudinal trace le parcours professionnel d'une jeune femme de l'âge de 17 ans à 23 ans. En se basant sur la littérature sur les phases de transitions de la jeunesse et sur l'érudition féministe sur la division du travail selon les sexes, cet article considère la façon dont sa trajectoire a été façonnée par des idéologies puissantes reliées au travail relationnel, la production au foyer et à l'éducation postsecondaire

    Direct measurement of transcription rates reveals multiple mechanisms for configuration of the Arabidopsis ambient temperature response

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    Background Sensing and responding to ambient temperature is important for controlling growth and development of many organisms, in part by regulating mRNA levels. mRNA abundance can change with temperature, but it is unclear whether this results from changes in transcription or decay rates, and whether passive or active temperature regulation is involved. Results Using a base analog labelling method, we directly measured the temperature coefficient, Q10, of mRNA synthesis and degradation rates of the Arabidopsis transcriptome. We show that for most genes, transcript levels are buffered against passive increases in transcription rates by balancing passive increases in the rate of decay. Strikingly, for temperature-responsive transcripts, increasing temperature raises transcript abundance primarily by promoting faster transcription relative to decay and not vice versa, suggesting a global transcriptional process exists that controls mRNA abundance by temperature. This is partly accounted for by gene body H2A.Z which is associated with low transcription rate Q10, but is also influenced by other marks and transcription factor activities. Conclusions Our data show that less frequent chromatin states can produce temperature responses simply by virtue of their rarity and the difference between their thermal properties and those of the most common states, and underline the advantages of directly measuring transcription rate changes in dynamic systems, rather than inferring rates from changes in mRNA abundance. Background The mechanism for ambient temperature sensing in plants is unclear. Control of transcript levels is believed to be important in responses to temperature [1-4] but affects of ambient temperature on transcription and mRNA decay rates have not been measured. According to the work of Arrhenius [5] the temperature coefficient (Q10) of biochemical reactions is expected to be 2 to 3 at biological temperatures: yet less than 2% of Arabidopsis thaliana genes have a two-fold or greater difference in expression level between 17°C and 27°C [6]. The remaining genes either have rates buffered against changing temperatures, or passive increases in transcription rate must be offset by a balanced increase in decay rate, leading to higher turnover but static steady state levels. Despite this fundamental uncertainty, steady state transcriptomic responses to ambient temperature have been used to infer a role for chromatin modifications in temperature signaling [2,7]. 4-Thiouracil (4SU) is a non-toxic base analogue that has been shown to be incorporated into mammalian and yeast mRNA during transcription [8-12]. Biotinylation and column separation allow 4SU-labeled RNA to be separated from unlabeled RNA, and transcriptomic analysis using the separated samples can be used to simultaneously calculate mRNA synthesis and decay rates [8]. Here we use 4SU labeling to measure transcription rates and determine the Q10 genome-wide of mRNA synthesis and decay rates in Arabidopsis thaliana. We show that ambient temperature has large passive effects on both mRNA synthesis and decay rates, and that where temperature controls transcript abundance it does so by regulating transcription relative to decay and not vice versa. Our analysis suggests that transcription factor binding sites and epigenetic state combine to create a complex network of temperature responses in plants. Results Cells incorporate 4SU into RNA and this has been exploited in mammalian cells [8,11,12] and in yeast [13] to measure mRNA synthesis and decay rates. In order to determine whether plants can take up 4SU we floated intact seedlings in MS medium and monitored 4SU incorporation into RNA by biotinylation and dot blot (Figure S1a in Additional file 1). This clearly showed that plants incorporate 4SU from the environment into RNA and that concentrations as low as 1 mM lead to a signal detectable above background within 1 hour (Figure 1B). The resulting RNA could be separated from unlabeled RNA by biotinylation and passage through a streptavidin column as described previously. At 1.5 mM the flow-through can be depleted of detectable 4SU-labeled RNA, whilst labeled plant RNA is highly concentrated in the fraction recovered from the column [8,13] (Figure S1c in Additional file 1). To maximize recovery we chose a low concentration of 4SU at 1.5 mM [8] as high labeling frequencies are known to lead to binding of fewer more frequently labeled transcripts to the columns and reduce recovery. At this concentration Arabidopsis plants treated with 4SU showed the same growth and survival as control plants (Figure S2a in Additional file 1), suggesting 4SU has low toxicity in plants, as in other organisms. Therefore, 4SU dynamics in Arabidopsis seedlings resemble those described for other experimental systems. Preliminary experiments showed that RNA turnover was faster at 27°C compared to 12°C (Figure S2b in Additional file 1), suggesting that temperature generally affected transcription rates

    Shifting landscapes: from coalface to quick sand? Teaching geography, earth and environmental sciences in UK higher education

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    In this paper we examine contemporary academic working lives, with particular reference to teaching-only and teaching-focused academics. We argue that intensification in the neoliberal university has significantly shifted the structure of academic careers, while cultural stories about those careers have not changed. We call for academics to re-examine our collective stories about standard academic career paths. Challenging the stories and making visible the ways that they create and multiply disadvantage is a crucial step in expanding the possibilities for academic identities and careers. The paper begins by describing teaching-focused academics within the context of the wider workforce. We then draw on narratives of those in these roles to illustrate the processes that (re)inscribe their marginalisation. We uncover the gendering of the teaching-focused academic labour market. We end the paper by suggesting interventions that all academics can take and support to address the issues we highlight

    Community Legal Advice Centres: A Survey of Clients in Reception Areas

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