Long-term mesocosm simulation of algal and archaeal blooms in the Dead Sea following dilution with Red Sea water

To understand the factors that determine the extent of blooms of the unicellular green alga Dunaliella and halophilic Archaea in the Dead Sea, and to predict the possible effects of the planned conveyance of Red Sea water to the Dead Sea, we performed simulation experiments in the 0.9 m3 outdoor mesocosms on the grounds of the Dead Sea Works Ltd. at Sedom, as well as in the laboratory. The laboratory simulations showed that development of the Dunaliella was possible only when Dead Sea water (340 g 1-1 total dissolved salts) was diluted with minimally 10% (by volume) of Red Sea water (40 g 1-1 total dissolved salts). Addition of phosphate was essential for the algae to grow, and growth rates and yields increased with increasing phosphate concentration and decreasing salinity. Field simulations in the mesocosms showed that development of algae was rapidly followed by development of dense blooms of red halophilic Archaea, which imparted an intensely red color to the ponds. While algal numbers declined after the peak of the bloom had been reached, number of halophilic Archaea and levels of archaeal pigments remained high for over two years. Although it should be realized that the closed system formed by the shallow ponds differs from the conditions in the lake, the results suggest that a microbial bloom, once formed, can remain present in the Dead Sea for months to years. These observations are important when attempting to predict how the biological properties of the lake may change in the future, and they they have important implications for the planning of the Red Sea-Dead Sea conduit

Intra-Messinian truncation surface in the Levant Basin explained by subaqueous dissolution

The Messinian salinity crisis (MSC) is an extreme event in Earth history during which a salt giant (>1 × 106 km3) accumulated on the Mediterranean seafloor within ~640 k.y. Erosional unconformities extending from the continental margins into the deep basins are key features for reconstructing the MSC; however, the nature of the erosional processes and their subaerial versus subaqueous origin are highly controversial. This study focuses on the top erosion surface (TES) in the deep Levant Basin, which is notably flat, truncating a basinward-tilted Messinian evaporitic succession. Based on high-resolution seismic surveys and wireline logs, we show that (1) the TES is actually an intra-Messinian truncation surface (IMTS) located ~100 m below the Messinian-Zanclean boundary; (2) the topmost, post-truncation Messinian unit is very different from the underlying salt deposits and consists mostly of shale, sand, and anhydrite; and (3) the flat IMTS is a dissolution surface related to significant dilution and stratification of the water column during the transition from stage 2 to stage 3 of the MSC. Dissolution occurred upslope where salt rocks at the seabed were exposed to the upper diluted brine, while downslope, submerged in the deeper halite-saturated layer, the salt rocks were preserved. The model, which requires a stratified water column, is inconsistent with a complete desiccation of the eastern Mediterranean Sea

The onset of the Messinian salinity crisis in the deep Eastern Mediterranean basin

Astronomical tuning of the Messinian pre-salt succession in the Levant Basin allows for the first time the reconstruction of a detailed chronology of the Messinian salinity crisis (MSC) events in deep setting and their correlation with marginal records that supports the CIESM (2008) 3-stage model. Our main conclusions are (1) MSC events were synchronous across marginal and deep basins, (2) MSC onset in deep basins occurred at 5.97 Ma, (3) only foraminifera-barren, evaporite-free shales accumulated in deep settings between 5.97 and 5.60 Ma, (4) deep evaporites (anhydrite and halite) deposition started later, at 5.60 Ma and (5) new and published 87Sr/86Sr data indicate that during all stages, evaporites precipitated from the same water body in all the Mediterranean sub-basins. The wide synchrony of events and 87Sr/86Sr homogeneity implies inter-sub-basin connection during the whole MSC and is not compatible with large sea-level fall and desiccation of the Mediterranean

Vegetation history and climatic fluctuations on a transect along the Dead Sea west shore and impact on past societies over the last 3500 years.

This study represents the vegetation history of the last 3500 years and conducts an analysis of the climatic fluctuations on a 75 km long transect on the western Dead Sea shore. Palynological and sedimentological data are available from six cores near Mount Sedom, Ein Boqueq, and Ein Gedi and from outcrops near Ze'elim and Ein Feshkha. The comparison of the pollen data with the lake levels shows synchronous trends. During the Middle Bronze Age, Iron Age and Hellenistic to Byzantine Period the high lake level of the Dead Sea signals an increase in precipitation. Contemporaneously, values of cultivated plants indicate an increase in agriculture. Lake level is low during the Late Bronze Age, within the Iron Age and at the end of the Byzantine period, indicating dry periods when all pds show a decrease of cultivated plants. Forest regeneration led by drought-resistant pines is observed in all pollen diagrams (pds) following the agricultural decline in the Byzantine period and, in the pds near Ein Boqeq, Ze'elim and Ein Feshkha, during the late Iron Age. The modern vegetation gradient is reflected in the palaeo-records: a stronger expansion of Mediterranean vegetation and cultivated plants in the northern sites is recognisable

The Effect of Gentamicin-Induced Readthrough on a Novel Premature Termination Codon of CD18 Leukocyte Adhesion Deficiency Patients

Leukocyte adhesion deficiency 1 (LAD1) is an inherited disorder of neutrophil function. Nonsense mutations in the affected CD18 (ITB2) gene have rarely been described. In other genes containing such mutations, treatments with aminoglycoside types of antibiotics (e.g., gentamicin) were reported to partially correct the premature protein termination, by induction of readthrough mechanism.Genetic analysis was performed on 2 LAD1 patients. Expression, functional and immunofluorescence assays of CD18 in the patients were used to determine the in-vivo and in-vitro effects of gentamicin-induced readthrough. A theoretical modeling of the corrected CD18 protein was developed to predict the protein function.We found a novel premature termination codon, C562T (R188X), in exon 6 of the CD18 gene that caused a severe LAD1 phenotype in two unrelated Palestinian children. In-vivo studies on these patients' cells after gentamicin treatment showed abnormal adhesion and chemotactic functions, while in-vitro studies showed mislocalization of the corrected protein to the cytoplasm and not to the cell surface. A theoretical modeling of the corrected CD18 protein suggested that the replacement of the wild type arginine by gentamicin induced tryptophan at the position of the nonsense mutation, although enabled the expression of the entire CD18 protein, this was not sufficient to stabilize the CD18/11 heterodimer at the cell surface.A novel nonsense mutation in the CD18 gene causing a complete absence of CD18 protein and severe LAD1 clinical phenotype is reported. Both in vivo and in vitro treatments with gentamicin resulted in the expression of a corrected full-length dysfunctional or mislocalized CD18 protein. However, while the use of gentamicin increased the expression of CD18, it did not improve leukocyte adhesion and chemotaxis. Moreover, the integrity of the CD18/CD11 complex at the cell surface was impaired, due to abnormal CD18 protein and possibly lack of CD11a expression

Development of an in vitro test system for assessment of male, reproductive toxicity.

yesThere is a need for improved reproductive toxicology assays that do not require large numbers of animals but are sensitive and informative. Therefore, Staput velocity-sedimentation separation followed by culture of specific mouse testicular cells was used as such a system. The specificity of separation was assessed using immunocytochemistry to identify spermatids, spermatocytes and spermatogonia. The efficacy of the system to detect toxicity was then evaluated by analysing the effects of hydrogen peroxide (H2O2) by the terminal uridine-deoxynucleotide end-labelling (TUNEL) assay to show the rate of apoptosis induced among the different types of germ cells. We found that 2 h of treatment at both 1 and 10 μM induced increases of over ∼10-fold in the percentage of apoptotic cells (p ≤ 0.001), confirming that testicular germ cells are prone to apoptosis at very low concentrations of H2O2. It was also demonstrated for the first time for this compound that spermatogonia are significantly more susceptible than spermatocytes, which are more affected than spermatids. This reflects the proportion of actively dividing cells in these cell types, suggesting a mechanism for the differential sensitivity. The approach should thus form the basis of a useful test system for reproductive and genetic toxicology in the future