1,809 research outputs found

    In Vitro Culture of Bovine Preantral Follicles: A Review

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    Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral follicles and formation of early antral follicles from large (secondary) preantral follicles after in vitro culture. Therefore, this review will highlight the basic aspects of bovine folliculogenesis by focusing on preantral follicles, the methods of harvesting preantral follicles, the main results from in vitro follicular culture during the last 20 years, and the potential candidate substances (basic supplements, growth factors, and hormones) for improving the efficiency of in vitro follicle growth

    Trabalho infantil e saúde: perfil ocupacional e problemas músculo-esqueléticos

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    A dialética no pensamento antigo.

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    Long Term Tiller Population Dynamics in Swards of Grasses with Contrasting Persistence Strategy

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    The lifespan of individual grass tillers usually does not exceed 12-15 months, because of death of tillers after floral induction and development, or randomly from disease or other factors. Persistence of the tiller population over several years, and associated long term maintenance of the sward, thus depends on the rate of turnover of individual tillers. This study aimed to characterise seasonal and management conditions critical for tiller turnover and its components, tiller birth and tiller death. Two grasses were investigated: Festuca arundinacea and Lolium multiflorum, having high and low persistence, respectively

    Questionário sobre atenção à saúde dos idosos

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    Questionário que compõe o Módulo 1 “Análise Situacional” do curso de Especialização em Saúde da Família da UNA-SUS/UFPel. Permite uma visão da estrutura e do processo de trabalho da Unidade Básica de Saúde em relação ao Programa de Atenção à Saúde dos Idosos, incluindo a organização da oferta de ações preventivas e dos registros e a avaliação e o monitoramento das ações

    Preantral follicle population and distribution in the horse ovary.

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    Characterization of the ovarian preantral follicle population is a necessary step to improve understanding of folliculogenesis and ovarian physiology. Therefore, in the present study, the preantral follicle population in the equine ovary in young and old mares was investigated according to follicular morphology, follicular class, distance from the geometric center using ovarian maps, and follicular density within ovarian portions (lateral vs intermediary) and regions (dorsal vs ventral). Ovaries were collected from an abattoir and histologically processed for evaluation, and the follicle population was calculated. Overall, in the current detailed study, a higher preantral follicle population per mare ovary (mean: 82,206 ± 50,022; range: 1477 to 773,091) than originally reported was identified. Additionally, a mare age effect was observed in the follicle population (young: 152,664 vs old: 11,750) and the spatial distribution of morphologically normal and abnormal follicles and the density and population of follicular classes. These results demonstrate that, in addition to the preantral follicle population in the mare ovary being comparable to that of other species, the location and spatial distribution of these follicles is dynamic and varies depending on mare age and follicle status (i.e. morphology and developmental stage). The characterization of the distribution and population of preantral follicles in the mare ovary provided by this study can potentially aid in improving reproductive studies and assisted reproductive techniques and may expand the understanding of mechanisms involving ovarian plasticity and follicular migration. LAY SUMMARY: Knowledge of the distribution and population of immature eggs within follicles (preantral follicles) in the ovaries of mares can improve approaches to assisted reproductive techniques and fertility preservation. As the existing research on horse preantral follicle population was focused solely on large follicles, the present study provides an updated investigation of small and large preantral follicles in the mare, showing that the population is similar to those in other species. This study also shows that the way these follicles are distributed in the ovary varies depending on age and follicle characteristics. Results from this study may help to highlight which areas of the mare ovary should be looked at to find samples of good-quality follicles

    Mobilidade, imobilidade e a-mobilidade: para discutir o Turismo em tempos de COVID-19

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    Mobility has been revisited in recent years, associated with the themes of space, time, territory and place, but rarely involving Tourism, more directly. Analyzing Tourism under the mobility bias, broadens its theoretical scope, among others, by allowing to add to it the concepts of immobility and a-mobility. In these terms, this article aims to discuss the relations between Tourism and the triad Mobility, Immobility and A-mobility, reviewed against the backdrop of the Covid-19 Pandemic and the issues of social isolation associated with it. To this end, it introduces the concept of a-mobility, using the figure of the Paroptic as a metaphor. The investigation procedures resume a review carried out in databases, last June, using the terms <Tourism>, <Tourism>, <Coronavirus> and <Covid-19>, when the absence of the mobility issue was observed, in the corpus resulting from the initial review. At the present time, the question of mobility is taken up again. It is going that during the Pandemic and its immediate aftermath there was a crisis of and in mobility, dramatically affecting tourist practices. The crisis implies that displacements will gain new form and content in the near future, without abandoning mobility.La Movilidad ha sido revisada en años recientes, asociada a los temas espacio, tiempo, territorio y lugar, pero pocas veces involucrando el Turismo, más directamente. Analizar el Turismo bajo el sesgo de la movilidad amplía su alcance teórico, entre otros al permitir añadir a él los conceptos inmovilidad y a-movilidad. En consecuencia, el presente artículo tiene por objeto debatir las relaciones entre Turismo y la triada Movilidad, Imovilidad y A-movilidad, revisada en el contexto de la pandemia Covid-19 y las cuestiones de aislamiento social asociadas. Para ello introduce el concepto de movilidad, recurriendo a la figura del Panóptico como metáfora. Los procedimientos de investigación recogen revisiones realizadas en bases de datos, en junio pasado, utilizando los términos <Turismo>, <Tourism>, <Coronavirus> y <Covid-19>, cuando se constató la ausencia de la cuestión movilidad, en el corpus resultante de la revisión inicial. En la actualidad, se vuelve a plantear la cuestión del concepto de movilidad. Parece ser que durante la pandemia y después de ella se produjo una crisis de la y la movilidad, afectando de manera dramática a las prácticas turísticas. La crisis implica que los desplazamientos adquirirán nueva forma y contenido en un futuro próximo, sin abandonar la movilidad.A Mobilidade tem sido revisitada em anos recentes, associada aos temas espaço, tempo, território e lugar, mas poucas vezes envolvendo o Turismo, mais diretamente. Analisar o Turismo sob o viés da Mobilidade amplia seu escopo teórico, entre outros ao permitir acrescentar a ele os conceitos imobilidade e a-mobilidade. Nesses termos, o presente artigo objetiva discutir as relações entre Turismo e a tríade Mobilidade, Imobilidade e A-mobilidade, revista tendo como pano de fundo a Pandemia da COVID-19 e as questões do isolamento social a ela associadas. Para tal introduz o conceito A-mobilidade, recorrendo a figura do panóptico como metáfora. Os procedimentos de investigação retomam revisão realizadas em bases de dados, em junho último, utilizando os termos <Turismo>, <Tourism>, <Coronavirus> e <Covid-19>, quando foi constatada a ausência da questão mobilidade, no corpus resultante da revisão inicial. No presente momento, retoma-se a questão conceito mobilidade. Encaminha-se que durante a Pandemia e no seu após imediato registrou-se uma crise da e na mobilidade, afetando de modo dramático as práticas turísticas. A crise insinua que os deslocamentos ganharão nova forma e conteúdo no futuro próximo, sem abandonarem a mobilidade

    The Mare Model to Study the Effects of Ovarian Dynamics on Preantral Follicle Features.

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    Ovarian tissue collected by biopsy procedures allows the performance of many studies with clinical applications in the field of female fertility preservation. The aim of the present study was to investigate the influence of reproductive phase (anestrous vs. diestrous) and ovarian structures (antral follicles and corpus luteum) on the quality, class distribution, number, and density of preantral follicles, and stromal cell density. Ovarian fragments were harvested by biopsy pick-up procedures from mares and submitted to histological analysis. The mean preantral follicle and ovarian stromal cell densities were greater in the diestrous phase and a positive correlation of stromal cell density with the number and density of preantral follicles was observed. The mean area (mm2) of ovarian structures increased in the diestrous phase and had positive correlations with number of preantral follicles, follicle density, and stromal cell density. Biopsy fragments collected from ovaries containing an active corpus luteum had a higher follicle density, stromal cell density, and proportion of normal preantral follicles. In conclusion, our results showed: (1) the diestrous phase influenced positively the preantral follicle quality, class distribution, and follicle and stromal cell densities; (2) the area of ovarian structures was positively correlated with the follicle and stromal cell densities; and (3) the presence of an active corpus luteum had a positive effect on the quality of preantral follicles, and follicle and stromal densities. Therefore, herein we demonstrate that the presence of key ovarian structures favors the harvest of ovarian fragments containing an appropriate number of healthy preantral follicles
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