Preparation of Neurospora crassa mitochondria

The fungus Neurospora crassa represents a eukaryotic cell with high biosynthetic activities. Cell mass doubles in 2-4 hr during expone ntial growth , even in simple salt media with sucrose as the sole carbon source. The microorgani sm forms a mycelium of long hyphae durlng vegetative growth . The mitochondria can be isolated under relatively gentle condi tions since a few breaks in the threadlike hyphae are sufficient to cause the outflow of the organelles. This article describes two methods for the physical disruption of the hyphae : (I) The cell s are opened in a grind mill between two rotating corundum di sks. This is a continuous and fast procedure and allows large- and small-scale preparations of mitochondria. (2) Hyphae are ground with sand in a mortar and pestle. This procedure can be applied to microscale preparations of mitochondria starting with minute amounts of cells. Other procedures for the isolation of Neurospora mitochondria after the physical di sruption or the enzymatic degradation of the cell wall have been described elsewher

Density of States of GaAs-AlGaAs Heterostructures Deduced from Temperature Dependend Magnetocapacitance Measurements

Abstract We have analyzed the density of states of a two dimensional electron gas in a GaAs- AlGaAs hetereostructure by measuring the magnetocapacitance in magnetic fields up to 6 Tesla at temperatures below 10 K. The experimental data are well described by a Gaussian-like density of states where the linewidth à is proportional to B

Density of States in Landau Level Tails of GaAs-AlxGa1-xAs Heterostructures

From an analysis of the thermally activated resistivity as a function of the magnetic field in the quantum Hall regime we deduced the position of the Fermi energy in the mobility gap as a function of the filling factor and therefore the density of states. The measured density of states is best described by a Gaussian like profile superimposed on a constant background

Mesoscopic Spin-Boson Models of Trapped Ions

Trapped ions arranged in Coulomb crystals provide us with the elements to study the physics of a single spin coupled to a boson bath. In this work we show that optical forces allow us to realize a variety of spin-boson models, depending on the crystal geometry and the laser configuration. We study in detail the Ohmic case, which can be implemented by illuminating a single ion with a travelling wave. The mesoscopic character of the phonon bath in trapped ions induces new effects like the appearance of quantum revivals in the spin evolution.Comment: 4.4 pages, 5 figure

Euler buckling instability and enhanced current blockade in suspended single-electron transistors

Single-electron transistors embedded in a suspended nanobeam or carbon nanotube may exhibit effects originating from the coupling of the electronic degrees of freedom to the mechanical oscillations of the suspended structure. Here, we investigate theoretically the consequences of a capacitive electromechanical interaction when the supporting beam is brought close to the Euler buckling instability by a lateral compressive strain. Our central result is that the low-bias current blockade, originating from the electromechanical coupling for the classical resonator, is strongly enhanced near the Euler instability. We predict that the bias voltage below which transport is blocked increases by orders of magnitude for typical parameters. This mechanism may make the otherwise elusive classical current blockade experimentally observable.Comment: 15 pages, 10 figures, 1 table; published versio

Decoherence without dissipation?

In a recent article, Ford, Lewis and O'Connell (PRA 64, 032101 (2001)) discuss a thought experiment in which a Brownian particle is subjected to a double-slit measurement. Analyzing the decay of the emerging interference pattern, they derive a decoherence rate that is much faster than previous results and even persists in the limit of vanishing dissipation. This result is based on the definition of a certain attenuation factor, which they analyze for short times. In this note, we point out that this attenuation factor captures the physics of decoherence only for times larger than a certain time t_mix, which is the time it takes until the two emerging wave packets begin to overlap. Therefore, the strategy of Ford et al of extracting the decoherence time from the regime t < t_mix is in our opinion not meaningful. If one analyzes the attenuation factor for t > t_mix, one recovers familiar behaviour for the decoherence time; in particular, no decoherence is seen in the absence of dissipation. The latter conclusion is confirmed with a simple calculation of the off-diagonal elements of the reduced density matrix.Comment: 8 pages, 4 figure

Exploiting environmental resonances to enhance qubit quality factors

We discuss dephasing times for a two-level system (including bias) coupled to a damped harmonic oscillator. This system is realized in measurements on solid-state Josephson qubits. It can be mapped to a spin-boson model with a spectral function with an approximately Lorentzian resonance. We diagonalize the model by means of infinitesimal unitary transformations (flow equations), and calculate correlation functions, dephasing rates, and qubit quality factors. We find that these depend strongly on the environmental resonance frequency $\Omega$; in particular, quality factors can be enhanced significantly by tuning $\Omega$ to lie below the qubit frequency $\Delta$.Comment: 5 psges, 5 figure

Developing individual differences in primate behavior:The role of genes, environment and their interplay

As is the case for humans, it has long been thought that nonhuman primates can be described in terms of their personality. Scientific observations that support this view include the presence of individual differences in social behavior and that they are relatively stable throughout life. Consequently, individuals are constrained in their behavioral flexibility when dealing with various environmental challenges. Still, the variation among individuals during development suggests that the environment influences how primates behave. Research in fields including psychology, behavior genetics, and behavioral ecology have tried to identify the mechanisms responsible for this interplay of behavioral stability and change. In this review we integrate theories and findings from research on humans and nonhuman primates that highlight how and to what extent genetic and environmental contributions shape the development of social behavior. To do so we first provide an overview and define what is meant by mean level and rank-order change of behavior. We then review explanations of behavioral stability and change, focusing on the role of genetic effects, how environmental circumstances influence behavioral variation throughout development, and how genetic and environmental influences may interact to produce this variation. Finally, we point to future research directions that could help us to further understand the development of social behavior in primates from within a behavior genetics framework