Development of Degradable, pH‐Sensitive Star Vectors for Enhancing the Cytoplasmic Delivery of Nucleic Acids

The report describes the synthesis of degradable, pH‐sensitive, membrane‐destabilizing, star‐shaped polymers where copolymers of hydrophobic hexyl methacrylate (HMA) and 2‐(dimethylamino)ethyl methacrylate (DMAEMA) monomers are grafted from the secondary face of a beta‐cyclodextrin (β‐CD) core via acid‐labile hydrazone linkages using atom transfer radical polymerization. The effect of the graft's molecular weight, HMA/DMAEMA molar ratio, and the fraction of DMAEMA converted to cationic N,N,N‐trimethylaminoethyl methacrylate (TMAEMA) monomers on polymer's transfection capacity is systematically investigated. Results show that all star‐shaped polymers condense anti‐GAPDH silencing RNA (siRNA) into nanosized particles at +/‐ ratio ≤ 4:1. Star polymers with shorter (25kDa) P(HMA‐ co ‐DMAEMA‐ co ‐TMAEMA) grafts are more efficient and less cytotoxic than carriers with longer (40kDa) grafts. The results show that increasing the ratio of hydrophobic HMA monomers in graft's composition higher than 50 mole% dramatically reduces polymer's aqueous solubility and abolishes their transfection capacity. Further, retention of DMAEMA monomers in graft's composition provide a buffering capacity that enhanced the endosomal escape and transfection capacity of the polymers. These systematic studies show that β‐CD‐P(HMA‐ co ‐DMAEMA‐ co ‐TMAEMA) 4.8 polymer with a 25 kDa average graft's molecular weight and a 50/25/25 ratio of HMA/DMAEMA/TMAEMA monomers is the most efficient carrier in delivering the siRNA cargo into the cytoplasm of epithelial cancer cells. A series of degradable, pH‐sensitive, membrane‐destabilizing, star‐shaped polymers is synthesized. Star polymers are engineered to “sense” the drop in endosomal pH, which triggers the hydrolysis of acid‐labile hydrazone linkages and release of membrane‐active grafts that rupture the endosomal membrane and release the loaded siRNA cargo into the cytoplasm to produce the desired knockdown of targeted gene expression at both the mRNA and protein levels.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/99666/1/3885_ftp.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/99666/2/adfm_201203762_sm_suppl.pd

Open-Source Photovoltaic—Electrical Vehicle Carport Designs

Solar powering the increasing fleet of electrical vehicles (EV) demands more surface area than may be available for photovoltaic (PV)-powered buildings. Parking lot solar canopies can provide the needed area to charge EVs but are substantially costlier than roof- or ground-mounted PV systems. To provide a low-cost PV parking lot canopy to supply EV charging, in this study, we provide a full mechanical and economic analysis of three novel PV canopy systems: (1) an exclusively wood, single-parking-spot spanning system, (2) a wood and aluminum double-parking-spot spanning system, and (3) a wood and aluminum cantilevered system for curbside parking. All three systems can be scaled to any amount of EV parking spots. The complete designs and bill of materials (BOM) of the canopies are provided, along with basic instructions, and are released with an open-source license that will enable anyone to fabricate them. Analysis results indicate that single-span systems provide cost savings of 82–85%, double-span systems save 43–50%, and cantilevered systems save 31–40%. In the first year of operation, PV canopies can provide 157% of the energy needed to charge the least efficient EV currently on the market if it is driven the average driving distance in London, ON, Canada

Solar photovoltaic wood racking mechanical design for trellis-based agrivoltaics.

Using a trellis to plant vegetables and fruits can double or triple the yield per acre as well as reduce diseases/pests, ease harvesting and make cleaner produce. Cultivars such as cucumbers, grapes, kiwi, melons, peas, passion fruit, pole beans, pumpkins, strawberries, squash, and tomatoes are all grown with trellises. Many of these cultivars showed increased yield with partial shading with semi-transparent solar photovoltaic (PV) systems. To further increase the efficiency of trellis-based growing systems, this study investigates novel low-cost, open-source, sustainable, wood-based PV racking designs for agrivoltaic applications. Design calculations are made to ensure these racks exceed Canadian building code standards, which with snow loads surpass those of most of the world. A complete bill of materials, fabrication instructions, and proof-of-concept prototypes are provided for three system topographies (sloped, T-shaped and inverse Y) along with economic analysis. In addition, to being cost competitive, the designs can act as trellis supports and be used for irrigation/fertigation purposes. The results indicate that these racking structures have enormous promise both agriculturally and energetically. If employed on only grape farms inside Canada, 10 GW of PV potential is made available, which is more than twice the total current installed PV in Canada

Ballast-Supported Foundation Designs for Low-Cost Open-Source Solar Photovoltaic Racking

Although solar photovoltaic (PV) system costs have declined, capital cost remains a barrier to widespread adoption. Do-it-yourself (DIY) system designs can significantly reduce labor costs, but if they are not attached to a building structure, they require ground penetrating footings. This is not technically and economically feasible at all sites. To overcome these challenges, this study details systems designed to (1) eliminate drilling holes and pouring concrete, (2) propose solutions for both fixed and variable tilt systems, (3) remain cost effective, and (4) allow for modifications to best fit the user’s needs. The ballast-supported foundations are analyzed for eight systems by proposing two separate ballast designs: one for a single line of post systems, and one for a double line of post systems, both built on a 4-kW basis. The results of the analysis found that both designs are slightly more expensive than typical in-ground concrete systems by 25% (assuming rocks are purchased at a landscaping company), but the overall DIY system’s costs remain economically advantageous. Sensitivity analyses are conducted to show how modifications to the dimensions influence the weight of the system and thus change the economic value of the design, so users can trade dimensional freedom for cost savings, and vice versa. Overall, all wood-based PV racking system designs provide users with cost-effective and easy DIY alternatives to conventional metal racking, and the novel ballast systems presented provide more versatility for PV systems installations

Open-Source Photovoltaic—Electrical Vehicle Carport Designs

Solar powering the increasing fleet of electrical vehicles (EV) demands more surface area than may be available for photovoltaic (PV)-powered buildings. Parking lot solar canopies can provide the needed area to charge EVs but are substantially costlier than roof- or ground-mounted PV systems. To provide a low-cost PV parking lot canopy to supply EV charging, in this study, we provide a full mechanical and economic analysis of three novel PV canopy systems: (1) an exclusively wood, single-parking-spot spanning system, (2) a wood and aluminum double-parking-spot spanning system, and (3) a wood and aluminum cantilevered system for curbside parking. All three systems can be scaled to any amount of EV parking spots. The complete designs and bill of materials (BOM) of the canopies are provided, along with basic instructions, and are released with an open-source license that will enable anyone to fabricate them. Analysis results indicate that single-span systems provide cost savings of 82–85%, double-span systems save 43–50%, and cantilevered systems save 31–40%. In the first year of operation, PV canopies can provide 157% of the energy needed to charge the least efficient EV currently on the market if it is driven the average driving distance in London, ON, Canada

Wood- and Cable-Based Variable Tilt Stilt-Mounted Solar Photovoltaic Racking System

The prohibitive costs of small-scale solar photovoltaic (PV) racks decrease PV adoption velocity. To overcome these costs challenges, an open hardware design method is used to develop two novel variable tilt racking designs. These are the first stilt-mounted racking designs that allow for the manual change of the tilt angle from zero to 90 degrees by varying the length of cables. The racks are designed using the calculated dead, wind, and snow loads for Canada as a conservative design for most of the rest of the world. Structural capacities of the wooden members are then ascertained and the resisting bending moment, shear force, tensile force, and compressive force is calculated for them. A structural and truss analysis is performed to ensure that the racking design withstands the applicable forces. Moreover, the implications of changing the tilt angle on the wooden members/cables used to build the system are also determined. The systems offer significant economic savings ranging from one third to two thirds of the capital expenses of the commercially available alternatives. In addition, the racking designs are easy-to-build and require minimal manufacturing operations, which increases their accessibility. The stilt-mounted designs can be employed for agrivoltaic settings while allowing farm workers shaded, ergonomic access to perform planting, weeding, and harvesting

Lions, hyenas and mobs (Oh my!)

Understanding the factors that facilitate the emergence of cooperation among organisms is central to the study of social evolution. Spotted hyenas Crocuta crocuta frequently cooperate to mob lions Panthera leo, approaching the lions as a tightknit group while vocalizing loudly in an attempt to overwhelm them and drive them away. Whereas cooperative mobbing behavior has been well documented in birds and some mammals, to our knowledge it has never been described during interactions between 2 apex predators. Using a 27-year dataset, we characterize lion-hyena encounters, assess rates of mobbing behavior observed during these interactions, and inquire whether mobbing results in successful acquisition of food. Lions and hyenas interacted most often at fresh kills, especially as prey size and the number of hyenas present increased. Possession of food at the beginning of an interaction positively affected retention of that food by each predator species. The presence of male lions increased the probability of an interspecific interaction but decreased the likelihood of hyenas obtaining or retaining possession of the food. Hyena mobbing rates were highest at fresh kills, but lower when adult male lions were present. The occurrence of mobbing was predicted by an increase in the number of hyenas present. Whether or not mobbing resulted in acquisition of food from lions was predicted by an increase in the number of mobs formed by the hyenas present, suggesting that cooperation among hyenas enhances their fitness

Heterogeneous expression of suppressor of cytokine signalling 2 (SOCS-2) in liver tissue

Suppressor of cytokine signalling 2 (SOCS-2), a dual effector of growth hormone signalling, was found to be heterogeneously expressed in murine liver parenchyma. Data from Affymetrix gene arrays, confirmed by quantitative RT-PCR using preparations of periportal and pericentral hepatocyte subpopulations as well as immunohistochemical detection, showed a preferential expression of SOCS-2 in pericentral hepatocytes. Stimulation of cultured periportal and pericentral hepatocyte subpopulations by different concentrations of growth hormone for 1 h resulted at 100 ng mL−1 in a 1.6-fold and 4.3-fold increase of SOCS-2 mRNA, respectively. Likewise, insulin-like growth factor-1, another physiological target of growth hormone, was stimulated preferentially in pericentral hepatocytes. As growth hormone receptor was found to be homogeneously expressed in mouse liver parenchyma, our data indicate that growth hormone signalling downstream of growth hormone receptor is more sensitive and/or effective in pericentral than in periportal hepatocytes. Presumably, the heterogeneous distribution of SOCS-2 may contribute to the pericentral preference of growth hormone action via differential feedback