Ontwikkelingen schurftherkenning fruit

In het EU-FP6 ISAFruit-project wordt een Crop Adapted Spray Application-systeem voor precisiegewasbescherming in de fruitteelt ontwikkeld. Het systeem garandeert een veilige toediening van gewasbeschermingsmiddelen in boomgaarden afgestemd op de grootte van de boom en de geldende weersomstandigheden. Het systeem wordt beschreve

Effect of air distribution and spray liquid distribution of a cross-flow fan orchard sprayer on spray deposition in fruit trees

In a 4-year ongoing research programme in the Netherlands, we focus to maximise spray deposition in pome fruit trees and minimise spray deposition underneath the trees on the ground and minimise spray drift. For a cross-flow fan orchard sprayer we therefore measured spray deposition in the tree as an effect of different air settings and nozzle types. Nozzle types chosen were the Albuz ATR lilac (as a reference) and the Albuz TVI8001, both sprayed at 7 bar spray pressure. The orchard sprayer was a Munckhof cross-flow fan sprayer with a 2.75 m high cross-flow construction on top of the axial fan, equipped with 8 nozzles on both sides. At a forward speed of 6.5 km/h the spray volume was 200 L/ha and 290 L/ha, for respectively the Albuz lilac and Albuz TVI8001 nozzles. Air settings were: High air setting - 540 rpm PTO; and Low air setting - 540 rpm, 400 rpm, and 300 rpm PTO. Liquid distributions were measured with an AAMS-Salvarani Vertical patternator with discs, and air distribution was measured with a self-constructed measuring device equipped with ultrasonic anemometers and a handheld vane-anemometer. Liquid distribution in the apple trees (cv. Elstar) was measured in the full leaf growing stage (following ISO 22522). First results show a good correlation between air distribution and liquid distribution. Vertical liquid distribution measured on the liquid measuring device correlates also very good with the liquid distribution at different heights in the tree. However, air distribution and especially air speed of the orchard sprayer showed that decreasing air assistance increased the spray deposition in the fruit trees. Showing that air assistance is an important parameter to be taken up in the advice to fruit growers.In a 4-year ongoing research programme in the Netherlands, we focus to maximise spray deposition in pome fruit trees and minimise spray deposition underneath the trees on the ground and minimise spray drift. For a cross-flow fan orchard sprayer we therefore measured spray deposition in the tree as an effect of different air settings and nozzle types. Nozzle types chosen were the Albuz ATR lilac (as a reference) and the Albuz TVI8001, both sprayed at 7 bar spray pressure. The orchard sprayer was a Munckhof cross-flow fan sprayer with a 2.75 m high cross-flow construction on top of the axial fan, equipped with 8 nozzles on both sides. At a forward speed of 6.5 km/h the spray volume was 200 L/ha and 290 L/ha, for respectively the Albuz lilac and Albuz TVI8001 nozzles. Air settings were: High air setting - 540 rpm PTO; and Low air setting - 540 rpm, 400 rpm, and 300 rpm PTO. Liquid distributions were measured with an AAMS-Salvarani Vertical patternator with discs, and air distribution was measured with a self-constructed measuring device equipped with ultrasonic anemometers and a handheld vane-anemometer. Liquid distribution in the apple trees (cv. Elstar) was measured in the full leaf growing stage (following ISO 22522). First results show a good correlation between air distribution and liquid distribution. Vertical liquid distribution measured on the liquid measuring device correlates also very good with the liquid distribution at different heights in the tree. However, air distribution and especially air speed of the orchard sprayer showed that decreasing air assistance increased the spray deposition in the fruit trees. Showing that air assistance is an important parameter to be taken up in the advice to fruit growers

Driftarme doppen voor de Opzetterteelt (laanbomen)

Een aantal middelen in de laanboomteelt (spillen en opzetters) mag alleen gebruikt worden met driftarme doppen. Onduidelijk is welke. De activiteiten binnen dit project geven hierin helderheid

Driftarme doppen voor de Spillenteelt (laanbomen)

Een aantal middelen in de laanboomteelt (spillen en opzetters) mag alleen gebruikt worden met driftarme doppen. Onduidelijk is welke. De activiteiten binnen dit project geven hierin helderheid

The AGE reader:a non-invasive method to assess long term tissue damage

AIMS: Advanced glycation endproducts (AGEs) are sugar modified adducts which arise during non-enzymatic glycoxidative stress. These compounds may become systemically elevated in disease states, and accumulate in tissue, especially on long-lived proteins. AGEs have been implicated in various acute, and chronic diseases, stressing the need for reliable and comprehensive measuring techniques. Measurement of AGEs in tissue such as skin requires skin biopsies, which is an invasive procedure. The AGE Reader has been developed to assess skin autofluorescence (SAF) non-invasively by the fluorescent properties of several AGEs. RESULTS/CONCLUSION: Various studies have shown that SAF is a useful marker of disease processes associated with oxidative stress. It is prospectively associated with development of cardiovascular events in patients with diabetes, renal or cardiovascular disease, and it predicts diabetes, cardiovascular disease and mortality in the general population. However, when measuring SAF in individual subjects, several factors may limit the reliability of the measurement, and hamper its use as a systemic biomarker for AGEs. These include endogenous factors present in the skin that absorb emission light such as melanin in dark-skinned subjects, but also factors that lead to temporal changes in SAF such as acute diseases and strenuous physical exercise associated with glycoxidative stress. Also, exogenous factors could potentially influence SAF levels inadvertently such as nutrition, and for example the application of skin care products. This review will give an overview of the AGE Reader functionality and the intrinsic, and exogenous factors which potentially influence the SAF assessment in individual subjects

Process-Oriented Profiling of Speech Sound Disorders

The differentiation between subtypes of speech sound disorder (SSD) and the involvement of possible underlying deficits is part of ongoing research and debate. The present study adopted a data-driven approach and aimed to identify and describe deficits and subgroups within a sample of 150 four to seven-year-old Dutch children with SSD. Data collection comprised a broad test battery including the Computer Articulation Instrument (CAI). Its tasks Picture Naming (PN), NonWord Imitation (NWI), Word and NonWord Repetition (WR; NWR) and Maximum Repetition Rate (MRR) each render a variety of parameters (e.g., percentage of consonants correct) that together provide a profile of strengths and weaknesses of different processes involved in speech production. Principal Component Analysis on the CAI parameters revealed three speech domains: (1) all PN parameters plus three parameters of NWI; (2) the remaining parameters of NWI plus WR and NWR; (3) MRR. A subsequent cluster analysis revealed three subgroups, which differed significantly on intelligibility, receptive vocabulary, and auditory discrimination but not on age, gender and SLPs diagnosis. The clusters could be typified as three specific profiles: (1) phonological deficit; (2) phonological deficit with motoric deficit; (3) severe phonological and motoric deficit. These results indicate that there are different profiles of SSD, which cover a spectrum of degrees of involvement of different underlying problems

Adaptation to high ethanol reveals complex evolutionary pathways

Tolerance to high levels of ethanol is an ecologically and industrially relevant phenotype of microbes, but the molecular mechanisms underlying this complex trait remain largely unknown. Here, we use long-term experimental evolution of isogenic yeast populations of different initial ploidy to study adaptation to increasing levels of ethanol. Whole-genome sequencing of more than 30 evolved populations and over 100 adapted clones isolated throughout this two-year evolution experiment revealed how a complex interplay of de novo single nucleotide mutations, copy number variation, ploidy changes, mutator phenotypes, and clonal interference led to a significant increase in ethanol tolerance. Although the specific mutations differ between different evolved lineages, application of a novel computational pipeline, PheNetic, revealed that many mutations target functional modules involved in stress response, cell cycle regulation, DNA repair and respiration. Measuring the fitness effects of selected mutations introduced in non-evolved ethanol-sensitive cells revealed several adaptive mutations that had previously not been implicated in ethanol tolerance, including mutations in PRT1, VPS70 and MEX67. Interestingly, variation in VPS70 was recently identified as a QTL for ethanol tolerance in an industrial bio-ethanol strain. Taken together, our results show how, in contrast to adaptation to some other stresses, adaptation to a continuous complex and severe stress involves interplay of different evolutionary mechanisms. In addition, our study reveals functional modules involved in ethanol resistance and identifies several mutations that could help to improve the ethanol tolerance of industrial yeasts