A comparative genetic diversity analysis in mungbean (Vigna radiata L.) using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP)

Amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat (ISSR) markers were used to study the DNA polymorphism in elite mungbean genotypes. A total of nine AFLP primer combination and 22 ISSR primers were used. Amplification of genomic DNA of the 30 genotypes, using AFLP analysis, yielded 300 fragments that could be scored, of which 192 were polymorphic, with an average of 21.3 polymorphic fragments per primer. Number of amplified fragments with AFLP primers ranged from 29 (E-AAC: M-CAG) to 10 (E-ACG: M-CAT). Percentage polymorphism ranged from 46.3% (E-AAC: M-CCA) to a maximum of 100% (E-AAC: M-CAC), with an average of 64%. The 22 ISSR primers used in the study produced 108 bands across 30 genotypes, of which 68 were polymorphic. The number of amplified bands varied from two UBC820) to ten URP 6F). The average numbers of bands per primer and polymorphic bands per primer were 4.9 and 3.1, respectively. Percentage polymorphism ranged from 25% (UBC844) to 85% (UBC846, UBC864, UBC895), with an average percentage polymorphism of 58.3% across all the genotypes. AFLP markers were more efficient than the ISSR assay, as they detected 64% polymorphic DNA markers in Vigna radiata as compared to 58.3% for ISSR markers. The Mantel test between the two Jaccard's similarity matrices gave r = 0.19, showing low correlation between AFLP- and ISSR-based similarities. Clustering of genotypes within groups was not similar when AFLP and ISSR derived dendrograms were compared.Key words: AFLP, ISSR, Vigna radiata (mung bean), marker index, unweighted pair-group method with arithmetic averages (UPGMA)

Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin

Biological and Soft Matter Physic

Omgaan met digitale nationale beleidskaarten

In dit werkdocument worden de resultaten besproken van een casestudy die onderdeel is van het project GeO3 - Omgaan met onzekerheid binnen Ruimtelijke Ordening. De directie Platteland van het ministerie van LNV heeft bij het publiceren van het meerjarenprogramma van Agenda Vitaal Platteland geen digitale viewer gepubliceerd omdat men bang was voor verkeerde interpretatie van de digitale kaarten. In dit project is gekeken naar methoden en cartografische oplossingen om voortaan zonder angst voor misinterpretaties digitale nationale beleidskaarten te kunnen verspreiden. De oplossing is gezocht in het opstellen van een handreiking, zodat kaarten ook daadwerkelijk weergeven wat er bedoeld is door de maker

Self-association of the spindle pole body-related intermediate filament protein Fin 1p and its phosphorylation-dependent interaction with 14-3-3 proteins in yeast

Moleculaire ontwikkelingsgenetic

Feasibility of assessment of skeletal muscle mass on a single cross-sectional image at the level of the fourth thoracic vertebra

Background Skeletal muscle mass (SMM) determined on computed tomography (CT) is emerging as a novel imaging biomarker. Cross-sectional area (CSA) of SMM at the level of the third lumbar vertebra (L3) on abdominal imaging is considered the clinical reference standard for measuring SMM. In certain patient groups, such as those with oncological or non-oncological lung disease like COVID-19, a chest CT may be available while an abdominal CT is not. The purpose of this study was to investigate whether determining SMM on a chest CT is a feasible alternative to abdominal CT.  Research question What is the correlation between SMM measurements at the level of L3 and the level of the fourth thoracic vertebra (Th4)?  Study design and methods  In this study we retrospectively analyzed abdominal and thoracic series of whole-body CT-scans of trauma patients (N = 47) and head and neck cancer patients (N = 194). All abdominal muscles were delineated on a single axial slice at the level of L3. The erector spinae, levator scapulae, rhomboideus minor and major and pectoralis minor and major muscles were delineated on a single axial slice at the level of Th4. CSA of the muscles at Th4 and the L3 level were compared using linear regression, and a multivariate linear regression model was established.  Results Muscle CSA at level Th4 strongly correlates with L3 muscle CSA (r = 0.791, p < 0.05). A multivariate model incorporating the patient characteristics arm positioning, age, sex, and weight achieved a stronger correlation (r = 0.856, p < 0.05). Interpretation: Skeletal muscle CSA measured at the level of Th4 is a feasible alternative to measurements at L3. This allows diagnosing low SMM using clinically available thoracic CT-scans. SMM measurements at the level of Th4 may become a prognostic or triage tool when faced with mechanical ventilator shortage

Thrombin-promoted release of UDP-glucose from human astrocytoma cells: Thrombin-promoted release of UDP-glucose

The P2Y14 receptor is activated by UDP-sugars, most potently by UDP-glucose, but not by free nucleotides, suggesting that UDP-glucose is the cognate agonist for this receptor. However, evidence for regulated release of UDP-glucose is scarce. In the present study, the occurrence of receptor-promoted release of UDP-glucose was investigated, using 1321N1 human astrocytoma cells