3,314 research outputs found
Clearing the Air on Radon Testing: The Duty of Real Estate Brokers to Protect Prospective Homebuyers
This Note recommends that the federal government create legislation that will impose a duty on real estate brokers to test homes for radon and to disclose the results to prospective purchasers. Based on a common law negligence theory, such a duty would become part of the current obligation of a real estate broker: (1) to conduct a reasonably diligent and competent search of property for sale; and (2) to disclose to prospective homebuyers all material defects affecting the value or desirability of the home. In his investigation, the broker must use the expertise and knowledge that derive from his training and experience as a professional. Initially, the Note addresses the dilemma of the homebuyer who discovers radon only after occupying the home and who has no formally defined cause of action based on common law precedent or statute. Part II traces the development of a real estate broker\u27s liability in negligence to the recently imposed duty to discover and disclose latent defects. Part III analyzes the duty to discover and disclose latent defects with respect to radon and concludes that real estate brokers should have an affirmative duty to test for radon and to disclose the results to prospective purchasers. Finally, part IV recommends legislation to protect the unwary homebuyer who otherwise would take possession of the home and suffer potential economic loss and exposure to a carcinogenic substance
Aquaculture induced erosion of tropical coastlines throws coastal communities back into poverty
Shallow tropical coastlines harbour unique mangrove ecosystems, which support livelihoods and provide a natural barrier against coastal flooding. Non-sustainable land-use practices, such as large-scale clear cutting of mangroves for aquaculture, ground water withdrawal and alteration of river flows, result in rapid subsidence. The collapse of aquaculture production, due to pollution and disease, is followed by coastal erosion, damage to infrastructure, intrusion of salt water and coastal flooding. Standard engineered interventions for protection often fail or are extremely expensive in these soft muddy environments. Subsidence and erosion render re-planting of mangroves in front of retreating coastlines impossible. Short-term solutions should focus on restoration of abiotic conditions, such as hydrology and sediment fluxes, to facilitate rapid establishment of protective mangrove belts. However, to ensure long-term sustainability, improved governance frameworks are required that put in place criteria for sustainable aquaculture, guide coastal infrastructure designs and limit ground water extraction
Nucleotide excision repair in yeast
Nucleotide Excision Repair (NER) is a conserved DNA repair pathway capable of removing a broad spectrum of DNA damage. In human cells a defect in NER leads to the disorder Xeroderma pigmentosum (XP). The yeast Saccharomyces cerevisiae is an excellent model organism to study the mechanism of NER. The yeast proteins Rad4 and Rad23 are important in NER and involved both Transcription-Coupled and Global Genome NER sub-pathways. Chapter 2 describes a novel mechanism of generegulation by the GG-NER E3 ligase. This protein complex can regulate dNTP synthesis via UV-induced Rad4 ubiquitination. In Chapter 3 we describe the effect of this histone on TC-NER at the rDNA. We find that Rad34 is only required when Histone H1 is present. In Chapter 4 we analyze the phenotype of a yeast strain containing a RAD4 mutation. The mutation affects a conserved residue that when altered in the human homolog XPC, leads to the cancer prone disorder XP. Chapter 5 is dedicated to the Rad4-Rad23 interaction that we describe in more detail. We uncovered a novel N-terminal interaction of Rad23 with Rad4 that is important for TC-NER. Finally, in Chapter 6 we reveal an unexpected UV phenotype for the commonly used wild-type yeast strain WCG4A. In this strain we identified the mutation to reside in the RAD4 geneUBL - phd migration 201
Hydrophobic lung surfactant proteins B and C remain associated with surface film during dynamic cyclic area changes
AbstractThe biophysical activity of lung surfactant depends, to a large extent, on the presence of the hydrophobic surfactant proteins B (SP-B) and C (SP-C). The role of these proteins in lipid adsorption and lipid squeeze-out under dynamic conditions simulating breathing is not yet clear. Therefore, the aim of this study was to investigate the interaction of spread hydrophobic surfactant proteins with phospholipids in a captive-bubble surfactometer during rapid cyclic area changes (6 cycles/min). We found that SP-B and SP-C facilitated the rapid transport of lipids into the air–water interface in a concentration-dependent manner (threshold concentration ≥0.05:0.5 mol% SP-B/SP-C). Successive rapid cyclic area changes did not affect the concentration-dependent lipid adsorption process, suggesting that SP-B and SP-C remained associated with the surface film
Neutron resonance capture applied to some prehistoric bronze axes
The elemental analysis of materials and objects on the basis of neutron resonance capture by nuclei as a function of neutron energy is briefly explained. The feasibility of neutron resonance capture analysis (NRCA) is demonstrated with five prehistoric “bronze” axes of different kinds and complex elemental compositions. Attention is paid to the occurrence of indium as a trace element in these artefacts
A patient perspective on information provision during the care path of Lentigo Maligna
Patients sometimes experience complex diagnostic and treatment procedures. During these processes, they need to rely on the information provided by the care providers. In particular, if they would like to play an active role in the shared decision-making process, it is important that this information is accessible, complete and understandable. A patient with Lentigo Maligna on the nose has been followed during the process of diagnosis, shared decision-making and treatment. Using the autoethnographical methodology, it was evaluated which sources of information available to the patient contributed to a better understanding, a more active role in the treatment process and a positive experience. Possible improvements are suggested.
Experience Framework
This article is associated with the Quality & Clinical Excellence lens of The Beryl Institute Experience Framework (https://www.theberylinstitute.org/ExperienceFramework). Access other PXJ articles related to this lens. Access other resources related to this lens
Isoforms of transferrin in psoriasis patients abusing alcohol
The different isoforms of transferrin have been quantified by isoelectric
focusing in the sera of psoriasis patients with and without a history of
abusing alcohol. In both male and female psoriasis subjects abusing
alcohol, there were significant increases in the 2-sialylated forms by
comparison to the control subjects. Psoriasis patients who had no evidence
of alcohol abuse had similar profile for the isoforms of transferrin to
that of the controls. Other groups of patients with alcohol-induced tissue
damage, i.e. liver, brain or muscle, used as positive controls, similarly
showed significant increases in the 2-sialylated forms, by comparison to
controls. These results substantiate the current use of
carbohydrate-deficient transferrin as a sensitive marker of alcohol abuse,
particularly in subjects not drinking in excess of 60 g of ethanol/day but
showing alcohol-related psoriasis
Prévisions agricoles. I. Méthodes, techniques et modèles = Agricultural forecasts. I. Methods, techniques and models. Internal information on agriculture 48, September 1969. 6736/VI/69-F
Gpnmb is a potential marker for the visceral pathology in Niemann-Pick type C disease
Impaired function of NPC1 or NPC2 lysosomal proteins leads to the intracellular accumulation of unesterified cholesterol, the primary defect underlying Niemann-Pick type C (NPC) disease. In addition, glycosphingolipids (GSLs) accumulate in lysosomes as well. Intralysosomal lipid accumulation triggers the activation of a set of genes, including potential biomarkers. Transcript levels of Gpnmb have been shown to be elevated in various tissues of an NPC mouse model. We speculated that Gpnmb could serve as a marker for visceral lipid accumulation in NPC disease. We report that Gpnmb expression is increased at protein level in macrophages in the viscera of Npc1nih/nih mice. Interestingly, soluble Gpnmb was also found to be increased in murine and NPC patient plasma. Exposure of RAW264.7 macrophages to the NPC-phenotype-inducing drug U18666A also upregulated Gpnmb expression. Inhibition of GSL synthesis with the glucosylceramide synthase (GCS) inhibitor N-butyl-1-deoxynojirimycin prevented U18666A-induced Gpnmb induction and secretion. In summary, we show that Gpnmb is upregulated in NPC mice and patients, most likely due to GSL accumulation
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