The Effects Of HIV Disease And Lifestyle Factors On Cellular Aging In Trangender Women

ABSTRACT THE EFFECTS OF HIV DISEASE AND LIFESTYLE FACTORS ON CELLULAR AGING IN TRANSGENDER WOMEN by Scott Stephen Sohn Background: Telomeres are short tandem repeats of nucleotides at the ends of chromosomes. These specialized structures serve as caps on the end of the chromosomes, which protect DNA integrity. Telomeres get shorter each time a cell replicates, but the DNA remains intact as long as the telomere caps are a sufficient length. In time, telomeres become too short to protect DNA, which leads to cellular death. Previous research has shown that disease and negative lifestyle factors play a role in accelerated telomere attrition throughout the cellular life cycle. Objective: The purpose of this study was to determine if HIV infection and lifestyle factors in a transgender population living in Atlanta Georgia are associated with telomere length reduction. Participants/setting: This study is a secondary analysis of data provided by a Georgia State University study entitled “Telomere Length, Environmental Stressors and Health Related Outcomes among Transgender Women”. The study included 92 transgender women from Atlanta, Georgia with 49 reporting HIV infection. Two sources of data were collected, survey responses collected during face to face interviews and a saliva sample for DNA analysis. Statistical analysis: Frequency statistics were used to describe the sample population. A Mann Whitney U was used to evaluate telomere length using the T/S ratio by HIV status, by physical activity level (healthy active or low active) and by fruit and vegetable intake category (Don’t eat, 1-2 servings/day, 3-4 servings/day vs. \u3e5 servings/day) in the total Population. Multiple regression analysis was used to examine the association between independent variables (activity level, body mass index, fruit and vegetable intake, hormone use, race, HIV status and age) and telomere length. Results: The majority of the population was Black (84%) with a median age of 33 years (range, 18 to 65 years). No significant association was observed between HIV infection and T/S ratio. The vast majority of the population reported low activity level and only 9% reported consuming \u3e5 servings of fruits and vegetables daily. No significant association was found between fruit and vegetable intake or physical activity level and T/S ratio in this population. Conclusion: HIV infection, Fruit and vegetable intake, and physical activity were not found to impact telomere length in an urban population of transgender women. Future research is needed to further understand the mechanisms that impact telomere length throughout the cellular life cycle within the transgender population

Arp 65 interaction debris: massive HI displacement and star formation

Context: Pre-merger interactions between galaxies can induce significant changes in the morphologies and kinematics of the stellar and ISM components. Large amounts of gas and stars are often found to be disturbed or displaced as tidal debris. This debris then evolves, sometimes forming stars and occasionally tidal dwarf galaxies. Here we present results from our HI study of Arp 65, an interacting pair hosting extended HI tidal debris. Aims: In an effort to understand the evolution of tidal debris produced by interacting pairs of galaxies, including in situ star and tidal dwarf galaxy formation, we are mapping HI in a sample of interacting galaxy pairs. The Arp 65 pair is one of them. Methods: Our resolved HI 21 cm line survey is being carried out using the Giant Metrewave Radio Telescope (GMRT). We used our HI survey data as well as available SDSS optical, Spitzer infra-red and GALEX UV data to study the evolution of the tidal debris and the correlation of HI with the star-forming regions within it. Results: In Arp 65 we see a high impact pre-merger interaction involving a pair of massive galaxies (NGC 90 and NGC 93) that have a stellar mass ratio of ~ 1:3. The interaction, which probably occurred ~ 1.0 -- 2.5 $\times$ 10$^8$ yr ago, appears to have displaced a large fraction of the HI in NGC 90 (including the highest column density HI) beyond its optical disk. We also find extended ongoing star formation in the outer disk of NGC 90. In the major star-forming regions, we find the HI column densities to be ~ 4.7 $\times$ 10$^{20}$ cm$^{-2}$ or lower. But no signature of star formation was found in the highest column density HI debris, SE of NGC 90. This indicates conditions within the highest column density HI debris remain hostile to star formation and it reaffirms that high HI column densities may be a necessary but not sufficient criterion for star formation.Comment: Accepted in A&

Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology.

Autism spectrum disorders (ASDs) are complex and heterogeneous developmental disabilities affecting an ever-increasing number of children worldwide. The diverse manifestations and complex, largely genetic aetiology of ASDs pose a major challenge to the identification of unifying neuropathological features. Here we describe the neurodevelopmental defects in mice that carry deleterious alleles of the Wdfy3 gene, recently recognized as causative in ASDs. Loss of Wdfy3 leads to a regionally enlarged cerebral cortex resembling early brain overgrowth described in many children on the autism spectrum. In addition, affected mouse mutants display migration defects of cortical projection neurons, a recognized cause of epilepsy, which is significantly comorbid with autism. Our analysis of affected mouse mutants defines an important role for Wdfy3 in regulating neural progenitor divisions and neural migration in the developing brain. Furthermore, Wdfy3 is essential for cerebral expansion and functional organization while its loss-of-function results in pathological changes characteristic of ASDs

Astrometry with the Wide-Field InfraRed Space Telescope

The Wide-Field InfraRed Space Telescope (WFIRST) will be capable of delivering precise astrometry for faint sources over the enormous field of view of its main camera, the Wide-Field Imager (WFI). This unprecedented combination will be transformative for the many scientific questions that require precise positions, distances, and velocities of stars. We describe the expectations for the astrometric precision of the WFIRST WFI in different scenarios, illustrate how a broad range of science cases will see significant advances with such data, and identify aspects of WFIRST's design where small adjustments could greatly improve its power as an astrometric instrument.Comment: version accepted to JATI

A Complete Spectroscopic Survey of the Milky Way Satellite Segue 1: The Darkest Galaxy

We present the results of a comprehensive Keck/DEIMOS spectroscopic survey of the ultra-faint Milky Way satellite galaxy Segue 1. We have obtained velocity measurements for 98.2% of the stars within 67 pc (10 arcmin, or 2.3 half-light radii) of the center of Segue 1 that have colors and magnitudes consistent with membership, down to a magnitude limit of r=21.7. Based on photometric, kinematic, and metallicity information, we identify 71 stars as probable Segue 1 members, including some as far out as 87 pc. After correcting for the influence of binary stars using repeated velocity measurements, we determine a velocity dispersion of 3.7^{+1.4}_{-1.1} km/s, with a corresponding mass within the half-light radius of 5.8^{+8.2}_{-3.1} x 10^5 Msun. The stellar kinematics of Segue 1 require very high mass-to-light ratios unless the system is far from dynamical equilibrium, even if the period distribution of unresolved binary stars is skewed toward implausibly short periods. With a total luminosity less than that of a single bright red giant and a V-band mass-to-light ratio of 3400 Msun/Lsun, Segue 1 is the darkest galaxy currently known. We critically re-examine recent claims that Segue 1 is a tidally disrupting star cluster and that kinematic samples are contaminated by the Sagittarius stream. The extremely low metallicities ([Fe/H] < -3) of two Segue 1 stars and the large metallicity spread among the members demonstrate conclusively that Segue 1 is a dwarf galaxy, and we find no evidence in favor of tidal effects. We also show that contamination by the Sagittarius stream has been overestimated. Segue 1 has the highest measured dark matter density of any known galaxy and will therefore be a prime testing ground for dark matter physics and galaxy formation on small scales.Comment: 24 pages, 4 tables, 11 figures (10 in color). Submitted for publication in ApJ. V3 revised according to comments from the refere

The Protein Kinase Tor1 Regulates Adhesin Gene Expression in Candida albicans

Eukaryotic cell growth is coordinated in response to nutrient availability, growth factors, and environmental stimuli, enabling cell–cell interactions that promote survival. The rapamycin-sensitive Tor1 protein kinase, which is conserved from yeasts to humans, participates in a signaling pathway central to cellular nutrient responses. To gain insight into Tor-mediated processes in human fungal pathogens, we have characterized Tor signaling in Candida albicans. Global transcriptional profiling revealed evolutionarily conserved roles for Tor1 in regulating the expression of genes involved in nitrogen starvation responses and ribosome biogenesis. Interestingly, we found that in C. albicans Tor1 plays a novel role in regulating the expression of several cell wall and hyphal specific genes, including adhesins and their transcriptional repressors Nrg1 and Tup1. In accord with this transcriptional profile, rapamycin induced extensive cellular aggregation in an adhesin-dependent fashion. Moreover, adhesin gene induction and cellular aggregation of rapamycin-treated cells were strongly dependent on the transactivators Bcr1 and Efg1. These findings support models in which Tor1 negatively controls cellular adhesion by governing the activities of Bcr1 and Efg1. Taken together, these results provide evidence that Tor1-mediated cellular adhesion might be broadly conserved among eukaryotic organisms

Cryopreservation Effect on Proliferative and Chondrogenic Potential of Human Chondrocytes Isolated from Superficial and Deep Cartilage

[Abstract] Objectives: To compare the proliferative and chondrogenic potential of fresh and frozen chondrocytes isolated from superficial and deep articular cartilage biopsies. Materials and Methodology: The study included 12 samples of fresh and frozen healthy human knee articular cartilage. Cell proliferation was tested at 3, 6 and 9 days. Studies of mRNA quantification, protein expression and immunofluorescence for proliferation and chondrogenic markers were performed. Results: Stimulation of fresh and frozen chondrocytes from both superficial and deep cartilage with fetal bovine serum produced an increase in the proliferative capacity compared to the non-stimulated control group. In the stimulated fresh cells group, the proliferative capacity of cells from the deep biopsy was greater than that from cells from the superficial biopsy (0.046 vs 0.028, respectively, p<0.05). There was also a significant difference between the proliferative capacity of superficial zone fresh (0.028) and frozen (0.051) chondrocytes (p<0.05). CCND1 mRNA and protein expression levels, and immunopositivity for Ki67 revealed a higher proliferative capacity for fresh articular chondrocytes from deep cartilage. Regarding the chondrogenic potential, stimulated fresh cells showed higher SOX9 and Col II expression in chondrocytes from deep than from superficial zone (p<0.05, T student test). Conclusions: The highest rate of cell proliferation and chondrogenic potential of fresh chondrocytes was found in cells obtained from deep cartilage biopsies, whereas there were no statistically significant differences in proliferative and chondrogenic capacity between biopsy origins with frozen chondrocytes. These results indicate that both origin and cryopreservation affect the proliferative and chondrogenic potential of chondrocytes.Servizo Galego de Saúde; PS07/84Instituto de Salud Carlos III; CIBER BBN CB06-01-0040Ministerio Ciencia e Innovacion; PLE2009-0144Ministerio Ciencia e Innovación; PI 08/202

Perspectives of San Juan healthcare practitioners on the detection deficit in oral premalignant and early cancers in Puerto Rico: a qualitative research study

<p>Abstract</p> <p>Background</p> <p>In Puerto Rico, relative to the United States, a disparity exists in detecting oral precancers and early cancers. To identify factors leading to the deficit in early detection, we obtained the perspectives of San Juan healthcare practitioners whose practice could be involved in the detection of such oral lesions.</p> <p>Methods</p> <p>Key informant (KI) interviews were conducted with ten clinicians practicing in or around San Juan, Puerto Rico. We then triangulated our KI interview findings with other data sources, including recent literature on oral cancer detection from various geographic areas, current curricula at the University of Puerto Rico Schools of Medicine and Dental Medicine, as well as local health insurance regulations.</p> <p>Results</p> <p>Key informant-identified factors that likely contribute to the detection deficit include: many practitioners are deficient in knowledge regarding oral cancer and precancer; oral cancer screening examinations are limited regarding which patients receive them and the elements included. In Puerto Rico, specialists generally perform oral biopsies, and patient referral can be delayed by various factors, including government-subsidized health insurance, often referred to as Reforma. Reforma-based issues include often inadequate clinician knowledge regarding Reforma requirements/provisions, diagnostic delays related to Reforma bureaucracy, and among primary physicians, a perceived financial disincentive in referring Reforma patients.</p> <p>Conclusions</p> <p>Addressing these issues may be useful in reducing the deficit in detecting oral precancers and early oral cancer in Puerto Rico.</p