A first assessment of genetic variability in the longhorn beetle Rosalia alpina (Coleoptera: Cerambycidae) from the Italian Apennines

The Rosalia longicorn (Rosalia alpina) is a strictly protected saproxylic beetle, widely distributed in Central and Southern Europe and mainly associated with ancient beech forests. To improve knowledge about the conservation status of R. alpina in Italy, available molecular markers (microsatellites and mitochondrial cytochrome c oxidase I(COI)) were tested for the first time on Italian populations. The study was performed in four sampling sites distributed in two areas placed in Northern (“Foreste Casentinesi” National Park) and Central Apennines (“Abruzzo, Lazio and Molise” National Park) where populational data about Rosalia longicorn were collected in the framework of the European LIFE MIPP Project. The genetic relationship among Apennine and Central/South-eastern European populations was explored by a comparison with mitochondrial DNA (mtDNA) data from literature. Microsatellite markers were only partially informative when applied to R. alpina Italian individuals, although providing some preliminary indication on an extensive gene flow among populations from the Apennines and local ongoing processes of genetic erosion. Genetic data are consistent with previous ecological data suggesting that the maintenance of variability in this species could be related to both habitat continuity and preservation of large senescent or standing dead trees in forests. Finally, a peculiar origin of the Apennine populations of R. alpina from a putative “Glacial Refugium” in Italy was inferred through COI data. The high genetic distance scored among the analysed populations and those from Central and South-eastern Europe indicates that the R. alpina deme from Apennine Mountains might represent a relevant conservation unit in Europe. Further genetic analyses will allow assessing other possible conservation units of R. alpina and, thus, defining large-scale conservation strategies to protect this endangered longhorn beetle in Europe

Grandes branquiópodos (Crustacea: Branchiopoda: Anostraca, Notostraca) en la provincia de Málaga (España) (año hidrológico 2012/2013)

Grans branquiòpodes (Crustacea, Branchiopoda: Anostraca, Notostraca) a la província de Màlaga, Espanya (any hidrològic 2012/2013) S'enumeren les cites d'una campanya de mostratge de grans branquiòpodes portada a terme a la província de Màlaga (Andalusia, sud d'Espanya) que ha permès la detecció de cinc espècies (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. i Phallocryptus spinosa) en 90 masses d'aigua mostrejades.Large branchiopods (Crustacea, Branchiopoda, Anostraca, Notostraca) from Málaga province, Spain (2012/2013 hydrological year) This paper presents the occurrence of the large branchiopods detected during a survey carried out in the province of Málaga (Andalusia, southern Spain). Five species (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. and Phallocryptus spinosa) were recorded at 90 sampled wetlands.Se enumeran las citas de una campaña de muestreo de grandes branquiópodos realizada en la provincia de Málaga (Andalucía, sur de España) que ha permitido la detección de cinco especies (Branchipus cortesi, Chirocephalus diaphanus, Streptocephalus torvicornis, Triops mauritanicus aggr. y Phallocryptus spinosa) en 90 masas de agua muestreadas

Acute febrile illness is associated with Rickettsia spp infection in dogs

BACKGROUND: Rickettsia conorii is transmitted by Rhipicephalus sanguineus ticks and causes Mediterranean Spotted Fever (MSF) in humans. Although dogs are considered the natural host of the vector, the clinical and epidemiological significance of R. conorii infection in dogs remains unclear. The aim of this prospective study was to investigate whether Rickettsia infection causes febrile illness in dogs living in areas endemic for human MSF. METHODS: Dogs from southern Italy with acute fever (n = 99) were compared with case–control dogs with normal body temperatures (n = 72). Serology and real-time PCR were performed for Rickettsia spp., Ehrlichia canis, Anaplasma phagocytophilum/A. platys and Leishmania infantum. Conventional PCR was performed for Babesia spp. and Hepatozoon spp. Acute and convalescent antibodies to R. conorii, E. canis and A. phagocytophilum were determined. RESULTS: The seroprevalence rates at first visit for R. conorii, E. canis, A. phagocytophilum and L. infantum were 44.8%, 48.5%, 37.8% and 17.6%, respectively. The seroconversion rates for R. conorii, E. canis and A. phagocytophilum were 20.7%, 14.3% and 8.8%, respectively. The molecular positive rates at first visit for Rickettsia spp., E. canis, A. phagocytophilum, A. platys, L. infantum, Babesia spp. and Hepatozoon spp. were 1.8%, 4.1%, 0%, 2.3%, 11.1%, 2.3% and 0.6%, respectively. Positive PCR for E. canis (7%), Rickettsia spp. (3%), Babesia spp. (4.0%) and Hepatozoon spp. (1.0%) were found only in febrile dogs. The DNA sequences obtained from Rickettsia and Babesia PCRs positive samples were 100% identical to the R. conorii and Babesia vogeli sequences in GenBank®, respectively. Febrile illness was statistically associated with acute and convalescent positive R. conorii antibodies, seroconversion to R. conorii, E. canis positive PCR, and positivity to any tick pathogen PCRs. Fourteen febrile dogs (31.8%) were diagnosed with Rickettsia spp. infection based on seroconversion and/or PCR while only six afebrile dogs (12.5%) seroconverted (P = 0.0248). The most common clinical findings of dogs with Rickettsia infection diagnosed by seroconversion and/or PCR were fever, myalgia, lameness, elevation of C-reactive protein, thrombocytopenia and hypoalbuminemia. CONCLUSIONS: This study demonstrates acute febrile illness associated with Rickettsia infection in dogs living in endemic areas of human MSF based on seroconversion alone or in combination with PCR

Mesoscopic superpositions of vibronic collective states of N trapped ions

We propose a scalable procedure to generate entangled superpositions of motional coherent states and electronic states in N trapped ions. Beyond their fundamental importance, these states may be of interest for quantum information processing and may be used in experimental studies of decoherence.Comment: Final version, as published in Physical Review Letters. See also further developments and applications in quant-ph/020207

49Cr: Towards full spectroscopy up to 4 MeV

The nucleus 49Cr has been studied analysing gamma-gamma coincidences in the reaction 46Ti(alpha,n)49Cr at the bombarding energy of 12 MeV. The level scheme has been greatly extended at low excitation energy and several new lifetimes have been determined by means of the Doppler Shift Attenuation Method. Shell model calculations in the full pf configuration space reproduce well negative-parity levels. Satisfactory agreement is obtained for positive parity levels by extending the configuration space to include a nucleon-hole either in the 1d3/2 or in the 2s1/2 orbitals. A nearly one-to-one correspondence is found between experimental and theoretical levels up to an excitation energy of 4 MeV. Experimental data and shell model calculations are interpreted in terms of the Nilsson diagram and the particle-rotor model, showing the strongly coupled nature of the bands in this prolate nucleus. Nine values of K(pi) are proposed for the levels observed in this experiment. As a by-result it is shown that the values of the experimental magnetic moments in 1f7/2 nuclei are well reproduced without quenching the nucleon g-factors.Comment: 13 pages, 8 figure

Guidelines for the monitoring of Rosalia alpina

Copyright Alessandro Campanaro et al. Rosalia alpina (Linnaeus, 1758) is a large longhorn beetle (Coleoptera: Cerambycidae) which is protected by the Habitats Directive and which typically inhabits beech forests characterised by the presence of mature, dead (or moribund) and sun-exposed trees. A revision of the current knowledge on systematics, ecology and conservation of R. alpina is reported. The research was carried out as part of the LIFE MIPP project which aims to find a standard monitoring method for saproxylic beetles protected in Europe. For monitoring this species, different methods were tested and compared in two areas of the Apennines, utilising wild trees, logs and tripods (artificially built with beech woods), all potentially suitable for the reproduction of the species. Even if all methods succeeded in the survey of the target species, these results showed that the use of wild trees outperformed other methods. Indeed, the use of wild trees allowed more adults to be observed and required less intensive labour. However, monitoring the rosalia longicorn on wild trees has the main disadvantage that they can hardly be considered “standard sampling units”, as each tree may be differently attractive to adults. Our results demonstrated that the most important factors influencing the attraction of single trunks were wood volume, sun-exposure and decay stage. Based on the results obtained during the project LIFE MIPP, as well as on a literature review, a standard monitoring method for R. alpina was developed

Modelling a high-mass red giant observed by CoRoT

The G6 giant HR\,2582 (HD\,50890) was observed by CoRoT for approximately 55 days. Mode frequencies are extracted from the observed Fourier spectrum of the light curve. Numerical stellar models are then computed to determine the characteristics of the star (mass, age, etc...) from the comparison with observational constraints. We provide evidence for the presence of solar-like oscillations at low frequency, between 10 and 20\,$\mu$Hz, with a regular spacing of $(1.7\pm0.1)\mu$Hz between consecutive radial orders. Only radial modes are clearly visible. From the models compatible with the observational constraints used here, We find that HR\,2582 (HD\,50890) is a massive star with a mass in the range (3--\,5\,$M_{\odot}$), clearly above the red clump. It oscillates with rather low radial order ($n$ = 5\,--\,12) modes. Its evolutionary stage cannot be determined with precision: the star could be on the ascending red giant branch (hydrogen shell burning) with an age of approximately 155 Myr or in a later phase (helium burning). In order to obtain a reasonable helium amount, the metallicity of the star must be quite subsolar. Our best models are obtained with a mixing length significantly smaller than that obtained for the Sun with the same physical description (except overshoot). The amount of core overshoot during the main-sequence phase is found to be mild, of the order of 0.1\,$H_{\rm p}$.Comment: Accepted in A&

Contrasting Population Structures of Two Vectors of African Trypanosomoses in Burkina Faso: Consequences for Control

Tsetse flies are insects that transmit trypanosomes to humans (sleeping sickness) and animals (nagana). Controlling these vectors is a very efficient way to control these diseases. In Burkina Faso, a tsetse eradication campaign is presently targeting the northern part of the Mouhoun River Basin. To attain this objective, the approach has to be area-wide, i.e. the control effort targets an entire pest population within a circumscribed area. To assess the level of this isolation, we studied the genetic structure of Glossina palpalis gambiensis and Glossina tachinoides populations in the target area and in the adjacent river basins of the Comoé, the Niger and the Sissili River Basins. Our results suggest an absence of strong genetic isolation of the target populations. We therefore recommend establishing permanent buffer zones between the Mouhoun and the other river basin(s) to prevent reinvasion. This kind of study may be extended to other areas on other tsetse species

Pre-multisymplectic constraint algorithm for field theories

We present a geometric algorithm for obtaining consistent solutions to systems of partial differential equations, mainly arising from singular covariant first-order classical field theories. This algorithm gives an intrinsic description of all the constraint submanifolds. The field equations are stated geometrically, either representing their solutions by integrable connections or, what is equivalent, by certain kinds of integrable m-vector fields. First, we consider the problem of finding connections or multivector fields solutions to the field equations in a general framework: a pre-multisymplectic fibre bundle (which will be identified with the first-order jet bundle and the multimomentum bundle when Lagrangian and Hamiltonian field theories are considered). Then, the problem is stated and solved in a linear context, and a pointwise application of the results leads to the algorithm for the general case. In a second step, the integrability of the solutions is also studied. Finally, the method is applied to Lagrangian and Hamiltonian field theories and, for the former, the problem of finding holonomic solutions is also analized.Comment: 30 pp. Presented in the International Workshop on Geometric Methods in Modern Physics (Firenze, April 2005

Population genetics of Glossina palpalis palpalis from central African sleeping sickness foci

<p>Abstract</p> <p>Background</p> <p><it>Glossina palpalis palpalis </it>(Diptera: Glossinidae) is widespread in west Africa, and is the main vector of sleeping sickness in Cameroon as well as in the Bas Congo Province of the Democratic Republic of Congo. However, little is known on the structure of its populations. We investigated <it>G. p. palpalis </it>population genetic structure in five sleeping sickness foci (four in Cameroon, one in Democratic Republic of Congo) using eight microsatellite DNA markers.</p> <p>Results</p> <p>A strong isolation by distance explains most of the population structure observed in our sampling sites of Cameroon and DRC. The populations here are composed of panmictic subpopulations occupying fairly wide zones with a very strong isolation by distance. Effective population sizes are probably between 20 and 300 individuals and if we assume densities between 120 and 2000 individuals per km², dispersal distance between reproducing adults and their parents extends between 60 and 300 meters.</p> <p>Conclusions</p> <p>This first investigation of population genetic structure of <it>G. p. palpalis </it>in Central Africa has evidenced random mating subpopulations over fairly large areas and is thus at variance with that found in West African populations of <it>G. p. palpalis</it>. This study brings new information on the isolation by distance at a macrogeographic scale which in turn brings useful information on how to organise regional tsetse control. Future investigations should be directed at temporal sampling to have more accurate measures of demographic parameters in order to help vector control decision.</p