Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication

In the past years, extracellular vesicles (EVs) have become an important field of research since EVs have been found to play a central role in biological processes. In pathogens, EVs are involved in several events during the host–pathogen interaction, including invasion, immunomodulation, and pathology as well as parasite–parasite communication. In this report, we summarised the role of EVs in infections caused by viruses, bacteria, fungi, protozoa, and helminths based on the talks and discussions carried out during the International Society for Extracellular Vesicles (ISEV) workshop held in São Paulo (November, 2016), Brazil, entitled Cross-organism Communication by Extracellular Vesicles: Hosts, Microbes and Parasites. © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.11Ysciescopu

Digestibilidade da ração de frangos de corte suplementados com probióticos e antibiótico Digestibility of broiler chickens diet supplemented with probiotics and antibiotics

O objetivo deste experimento foi avaliar a digestibilidade da ração de frangos de corte a qual continha ou não antibiótico e probióticos na fase inicial (um a 20 dias) e na fase final (21-40 dias). As aves foram distribuídas em um delineamento inteiramente casualizado com quatro tratamentos: Tratamento 1- Dieta Testemunha; Tratamento 2 - Dieta Testemunha + 0,02 % de probiótico 1 (Calsporin 10®), Tratamento 3 - Dieta Testemunha + 2,0% na fase inicial e 0,63% na fase final de probiótico 2 (Estibion aves®); Tratamento 4 - Dieta Testemunha + 0,013% de antibiótico (Bacitracina de Zinco®) e 5 repetições de 10 e 8 aves por unidade experimental por fase, respectivamente. Os pintos foram criados em gaiolas de metabolismo. Os resultados demonstram que a digestibilidade de matéria seca, nitrogênio e energia metabolizável aparente não foi afetada pela suplementação de antibiótico e probióticos na dieta de frangos de corte.<br>The objective of this experiment was to evaluate the diets digestibility in broiler chickens with and without antibiotic and probiotics, in the initial phase (one to 20 days) and finish phase (21 to 40 days). Chickens distributed in a completely randomized design were used in four treatments: Treatment 1- Control diet; Treatment 2 - Control diet + 0.02% probiotic 1 (Calsporin 10®), Treatment 3 - Control diet + 2.0% initial phase and 0.63% finish phase probiotic 2 (Estibion aves®); Treatment 4 - Control diet + 0.013% of antibiotic (Bacitracina de Zinco®), and five repetitions, with ten and eight birds, respectively, were used in each experimental unit, which were kept in metabolism cages. The results demonstrate that the digestibility of dry matter, nitrogen and apparent metabolized energy were not affected by the supplementation of antibiotic and probiotics in the broiler chickens diet

A tobacco cDNA reveals two different transcription patterns in vegetative and reproductive organs

In order to identify genes expressed in the pistil that may have a role in the reproduction process, we have established an expressed sequence tags project to randomly sequence clones from a Nicotiana tabacum stigma/style cDNA library. A cDNA clone (MTL-8) showing high sequence similarity to genes encoding glycine-rich RNA-binding proteins was chosen for further characterization. Based on the extensive identity of MTL-8 to the RGP-1a sequence of N. sylvestris, a primer was defined to extend the 5' sequence of MTL-8 by RT-PCR from stigma/style RNAs. The amplification product was sequenced and it was confirmed that MTL-8 corresponds to an mRNA encoding a glycine-rich RNA-binding protein. Two transcripts of different sizes and expression patterns were identified when the MTL-8 cDNA insert was used as a probe in RNA blots. The largest is 1,100 nucleotides (nt) long and markedly predominant in ovaries. The smaller transcript, with 600 nt, is ubiquitous to the vegetative and reproductive organs analyzed (roots, stems, leaves, sepals, petals, stamens, stigmas/styles and ovaries). Plants submitted to stress (wounding, virus infection and ethylene treatment) presented an increased level of the 600-nt transcript in leaves, especially after tobacco necrosis virus infection. In contrast, the level of the 1,100-nt transcript seems to be unaffected by the stress conditions tested. Results of Southern blot experiments have suggested that MTL-8 is present in one or two copies in the tobacco genome. Our results suggest that the shorter transcript is related to stress while the larger one is a flower predominant and nonstress-inducible messenger