Effect of Acidulated Phosphate Fluoride Gel Application Time on Enamel Demineralization of Deciduous and Permanent Teeth

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Although the effect of acidulated phosphate fluoride gel (APF gel) on caries reduction in permanent teeth is based on evidence, the relevance of the clinical application time is still under debate. Also, the effect of 4- versus 1-min application has not been evaluated in deciduous enamel. In a blind, crossover, in situ study of 14 days, 16 adult volunteers wore palatal appliances containing slabs of human permanent and deciduous enamel. At the beginning of each phase, the slabs were submitted to one of the following treatments: no APF application (negative control); APF gel (1.23% F) application for 1 or 4 min. Biofilm accumulation on the slab surface was allowed and the slabs were subjected eight times a day to 20% sucrose, simulating a high cariogenic challenge condition. On the 15th day of each phase, fluoride retained as CaF2 and fluorapatite (FAp) was determined on the enamel of the slabs and demineralization was assessed by cross-sectional microhardness. Fluoride as CaF2 and FAp, formed by APE gel application on the enamel slabs not subjected to the cariogenic challenge, was also determined. APF gel reduced demineralization in both enamel types (p 0.05). CaF2 and FAp formed and retained on deciduous and permanent enamel was significantly higher in APE gel groups (p 0.05). The findings suggest that 1 min of APF gel application provides a similar effect on inhibition of demineralization as 4 min, for both permanent and deciduous enamel. Copyright (C) 2012 S. Karger AG, Basel4613137Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPESP [2009/01785-6, 2010/20578-9

Factors affecting the in vitro embryo production in buffalo (Bubalus bubalis): A review

Under natural and well-managed conditions, the buffalo has good reproductive and productive indices. However, in vitro embryo production (IVEP) has been used commercially to maximise the number of elite animals. In this species, several factors (donor management, in vitro culture medium, semen, in vitro conditions, embryo transfer) still affect the IVEP results. In addition, the cost of this technique is very high for this purpose. Therefore, more studies, as well as adequate plans, are needed to achieve this objective efficiently. In this review, we discussed the current commercial status, influencing factors (in vivo and in vitro), and the progress and future challenges of IVEP in buffalo. A total of 81 references were used from 1979 to 2022. The relevant data or literature were searched using the following databases: Google, ResearchGate, Science Alert, Science Direct and PubMed, using the following keywords: buffalo oocytes/COCs, buffalo embryos, pregnancy and calving or live birth rate after embryo transfer. The best maturation, cleavage and blastocyst rates in the in vitro production of buffalo embryos were 95.8, 75.2 and 33.4%, respectively. The pregnancy and live birth rates ranged from 22.2% to 43.5% and from 15.3% to 36.5%, respectively, after the transfer of fresh embryos produced in vitro to the recipients. This review will help to contextualise IVEP in buffaloes, as well as create an adequate plan for implementing IVEP in buffaloes

Hydrogel and membrane scaffold formulations of Frutalin (breadfruit lectin) within a polysaccharide galactomannan matrix have potential for wound healing

Plant lectins are carbohydrate-binding proteins, which can interact with cell surfaces to initiate anti-inflammatory pathways, as well as immunomodulatory functions. Here, we have extracted, purified and part-characterized the bioactivity of Jacalin, Frutalin, DAL and PNA, before evaluating their potential for wound healing in cultured human skin fibroblasts. Only Frutalin stimulated fibroblast migration in vitro, prompting further studies which established its low cytotoxicity and interaction with TLR4 receptors. Frutalin also increased p-ERK expression and stimulated IL-6 secretion. The in vivo potential of Frutalin for wound healing was then assessed in hybrid combination with the polysaccharide galactomannan, purified from Caesalpinia pulcherrima seeds, using both hydrogel and membrane scaffolds formulations. Physical-chemical characterization of the hybrid showed that lectin-galactomannan interactions increased the pseudoplastic behaviour of solutions, reducing viscosity and increasing Frutalin's concentration. Furthermore, infrared spectroscopy revealed -OH band displacement, likely caused by interaction of Frutalin with galactose residues present on galactomannan chains, while average membrane porosity was 100 μm, sufficient to ensure water vapor permeability. Accelerated angiogenesis and increased fibroblast and keratinocyte proliferation were observed with the optimal hybrid recovering the lesioned area after 11 days. Our findings indicate Frutalin as a biomolecule with potential for tissue repair, regeneration and chronic wound healing

Características sócio-demográficas do atendimento ao idoso após alta hospitalar na Estratégia da Saúde de Família Características sociodemográfica y de la atención al anciano después de la alta hospitalaria en la Estrategia de la Salud de Familia Social demographic characteristics and the elderly care after hospital discharge in the Family Health System

Este estudo teve por objetivo verificar o perfil sócio-demográfico dos idosos e a assistência que recebem após a alta hospitalar da equipe da Estratégia de Saúde da Família (ESF). Trata-se de um estudo descritivo, e a coleta de dados foi realizada com 67 idosos que receberam alta nos meses de outubro, novembro e dezembro de 2007, residentes na área de abrangência da ESF do município de Marília (SP). Para apresentação dos dados, utilizou-se a estatística descritiva simples. A maioria dos idosos é do sexo feminino, e foi internada por encaminhamento do Pronto Socorro e, ao apresentar complicação, procurou o hospital. Mais de dois terços deles afirmaram ter recebido visita de profissionais da equipe da ESF, principalmente do Agente Comunitário de Saúde (ACS), mas sugeriram um acompanhamento mais frequente da equipe. Depreende-se que é preciso avançar na construção de um novo modelo de atenção ao idoso, após alta hospitalar.<br>En este estudio se propone verificar el perfil socio-demográfico de los ancianos y la atención que reciben luego del alta hospitalaria por parte del equipo de Estrategia de Salud de la Familia (ESF). Se trata de un estudio descriptivo, la recolección de datos fue realizada a partir de 67 ancianos residentes en el área de influencia de la ESF del municipio de Marília que habían recibido alta en los meses de octubre, noviembre y diciembre de 2007. Para el tratamiento de los datos, se utilizó la estadística descriptiva simple. La mayoría de los ancianos es de sexo femenino y, al experimentar complicaciones, se presentó en el hospital. Más de dos tercios de ellos afirmaron haber recibido visita de profesionales del equipo de ESF, en particular del Agente Comunitario de Salud (ACS), y sugirieron un seguimiento más frecuente del equipo. Se desprende que es preciso avanzar en la construcción de un nuevo modelo de atención.<br>The objective of this study was o verify the sociodemographic profile of the elderly and the health care service they receive from the Family Health Strategy (FHS) after their discharge. This is a descriptive study, and data collection was performed with 67 aged individuals who were discharged in October, November and December, 2007, and lived in the area covered by the FHS of Marília (São Paulo state). Simple descriptive analysis was used for the presentation of data. The majority of the elderly are female, and their hospitalization occurred as a referral of the Emergency Room due to complication. More than two thirds report they were visited by FHS team professionals, mainly the Community Health Agent (CHA), but they suggested the team should follow up closer. In conclusion, it is necessary to develop a new health care model for the elderly after hospital discharge