Como tratar linfoma hepatoesplênico de células T no SUS

O linfoma hepatoesplênico de células T (LHCT) é uma neoplasia rara e agressiva e não existe consenso no tratamento de primeira linha. O Sistema Único de Saúde (SUS), por se tratar se sistema público de saúde, nem sempre tem acesso aos tratamentos e métodos diagnósticos mais recentes. O presente artigo irá realizar revisão de literatura e trazer as alternativas disponíveis no SUS para manejo do LHCT

Erythrina velutina Willd. alkaloids : piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. Erythrina species are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids (BIAs), which can act on several pathology-related biological targets. Objectives: In this sense, in an unprecedented approach used with a non-model Fabaceae species grown in its unique arid natural habitat, a combined transcriptome and metabolome analyses (seeds and leaves) is presented. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a NextSeq 500 platform. Regarding metabolite profiling, the High-resolution Liquid Chromatography was coupled to DAD and a micrOTOF-QII mass spectrometer by using electrospray ionization (ESI) and Time of Flight (TOF) analyzer. The tandem MS/MS data were processed and analyzed through Molecular Networking approach. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids, several of them unique. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: Overall, these results could contribute by indicating potential biotechnological targets formodulation of erythrina alkaloids biosynthesis as well as improve molecular databases with omic data from a non-model medicinal plant, and reveal an interesting chemical diversity of Erythrina BIA harvested in Caatinga

Erythrina velutina Willd. alkaloids: Piecing biosynthesis together from transcriptome analysis and metabolite profiling of seeds and leaves

Introduction: Natural products of pharmaceutical interest often do not reach the drug market due to the associated low yields and difficult extraction. Knowledge of biosynthetic pathways is a key element in the development of biotechnological strategies for plant specialized metabolite production. The scarce studies regarding non-model plants impair advances in this field. Erythrina spp. are mainly used as central nervous system depressants in folk medicine and are important sources of bioactive tetracyclic benzylisoquinoline alkaloids, which can act on several pathology-related biological targets. Objective: Herein the purpose is to employ combined transcriptome and metabolome analyses (seeds and leaves) of a non-model medicinal Fabaceae species grown in its unique arid natural habitat. The study tries to propose a putative biosynthetic pathway for the bioactive alkaloids by using an omic integrated approach. Methods: The Next Generation Sequencing-based transcriptome (de novo RNA sequencing) was carried out in a Illumina NextSeq 500 platform. Regarding the targeted metabolite profiling, Nuclear Magnetic Resonance and the High-Performance Liquid Chromatography coupled to a micrOTOF-QII, High Resolution Mass Spectrometer, were used. Results: This detailed macro and micromolecular approach applied to seeds and leaves of E. velutina revealed 42 alkaloids by metabolome tools. Based on the combined evidence, 24 gene candidates were put together in a putative pathway leading to the singular alkaloid diversity of this species. Conclusion: These results contribute by indicating potential biotechnological targets Erythrina alkaloids biosynthesis as well as to improve molecular databases with omic data from a non-model medicinal plant. Furthermore, they reveal an interesting chemical diversity in Erythrina velutina harvested in Caatinga. Last, but not least, this data may also contribute to tap Brazilian biodiversity in a rational and sustainable fashion, promoting adequate public policies for preservation and protection of sensitive areas within the Caatinga

<b> Polynomial adjustment as a new technique for determination of lactate minimum velocity with blood sampling reduction </b>

The purpose of this study was to analyze the possibility of identifying the lactate minimum velocity (LM) and estimating the maximal lactate steady state intensity (MLSS) by applying a polynomial function to just three stages of the LM test. Seventeen physically active males (24.1 ± 4.0 years; 23.8 ±2.2kg.m2(-1) BMI; 11.7 ± 3.8% body fat) performed: 1) a 1600m time trial (1600mV); 2) a 150m sprint to induce hyperlactatemia, and then an incremental test (InT) consisting of 6 x 800m at intensities of 78, 81, 84, 87, 90 and 93% of 1600mV; 3) 2 to 3 sessions of constant 30 min running tests to identify MLSS. Blood lactate [lac] was determined by an electrochemical method (YSI - 2700 SELECT). The LM was identifi ed visually (LMv) as well by applying polynomial function to the [lac] responses at all 6 stages (LMp), to the 1st, 3rd and 5th stages (LMp135), to the 1st, 3rd and 6th stages (LMp136) and to the 1st, 4th and 6th stages (LMp146) of InT. The ANOVA detected no differences between the velocities (m.min-1) identifi ed by LMv (196.0 ± 17.8) LMp (198.0 ± 17.6), LMp135 (197.7 ± 17.6), LMp136 (200.0 ± 17.2), LMp146 (199,7 ± 18,1) and MLSS (198.7 ±16.6) (p>0.05), with a high correlation among each other (p<0.01). The polynomial function identifi ed LM even when applied to just 3 stages of the incremental test, enabling for prediction of MLSS intensity with a reduced number of blood samples being collected during testing. <p> <b> RESUMO</b> O propósito do estudo foi analisar a possibilidade de identifi cação da velocidade de lactato mínimo (LM) e de se estimar a máxima fase estável de lactato (MFEL), aplicando a função polinomial de segunda ordem a partir de apenas três estágios do teste do LM. Participaram do estudo 17 homens fi sicamente ativos (24,1 ± 4.0 anos; 23,8 ± 2,2kg.m2(-1) IMC; 11,7 ± 3,8% gordura corporal) realizaram: 1) corrida de 1600m no menor tempo possível para cálculo da velocidade média (1600mV); 2) “sprint” de 150m para indução de hiperlactatemia e um teste incremental (Tin) consistindo de 6x800m a intensidades de 78, 81, 84, 87, 90 e 93% do 1600mV; 3) 2 a 3 sessões de 30 min de corrida em intensidade constante para determinação da MFEL. O lactato sanguíneo [lac] foi determinado pelo método eletroenzimático (YSI - 2700 SELECT). O LM foi identifi cado visualmente (LMv) bem como aplicando ajuste polinomial usando todos os 6 estágios (LMp), o 1º, 3º e 5º estágios (LMp135), o 1º, 3º e 6º estágios (LMp136) e o 1º, 4º e 6º estágios (LMp146) do test incremental. ANOVA evidenciou não haver diferenças entre as velocidades (m.min-1) identifi cadas pelo LMv (196,0 ± 17,8), LMp (198,0 ± 17,6), LMp135 (197,7 ± 17,6), LMp136 (200,0 ±17,2), LMp146 (199,7 ±18,1) e MFEL (198,7 ± 16,6) (p>0,05), e alta correlação entre os métodos estudados (p<0,01). A identificação do LM aplicando a função polinomial em apenas 3 estágios, demonstrou ser válida em estimar a MFEL e útil por possibilitar redução no número de coletas de [lac] durante o teste

DÉFICIT COGNITIVO: PROPOSIÇÃO DE CARTILHA PARA ATENÇÃO AO IDOSO

ResumoIntrodução: dentre as formas mais comuns de perda de capacidade funcional verificada na população idosa, o déficit cognitivo chama a atenção pela dificuldade de diagnóstico e de tratamento. Os enfermeiros são profissionais de saúde com papel prioritário no apoio e suporte a essa população. Contudo, a área da enfermagem ainda é deficitária para oferecer assistência especializada aos idosos, particularmente com déficit cognitivo, sendo necessária a criação de novos instrumentos de assistência, almejando maior humanização em cuidados e permitindo a implementação de ações mais eficientes ao bem estar dessa população, garantindo um processo de envelhecimento digno dentro de suas limitações e/ou incapacidades, particularmente aos idosos comprometidos pelo déficit cognitivo. Objetivo: elaboração de uma cartilha de fácil aplicação para a atenção ao idoso com déficit cognitivo. Resultados: a presente cartilha é uma possibilidade e uma opção de alguns procedimentos importantes que devem ser atendidos em relação aos cuidados com idosos com déficit cognitivo. Conclusão: esse material não tem pretensões de ser algo definitivo, mais sim um dos primeiros a apresentar uma preocupação com os procedimentos necessários aos cuidados dispensados aos idosos com déficit cognitivo. Espera-se com isso incentivar a comunidade acadêmica a produzir material instrucional, bem como desenvolver mais estudos em relação aos cuidados e procedimentos voltados aos idosos com déficit cognitivo.Palavras-chave: Déficit Cognitivo; Idosos; Cuidados. AbstractIntroduction: among the most common forms of loss of functional capacity observed in the elderly, cognitive deficit calls attention to the difficulty of diagnosis and treatment. Nurses are health professionals with priority role in the support and support for this population. However, the nursing field is poor to provide expert assistance to the elderly, particularly cognitive impairment, necessitating the creation of new assistance instruments, aiming at greater humanization of care and enabling the implementation of more efficient actions to the well being of this population, guaranteeing a dignified aging process within their limitations and / or disabilities, particularly the elderly committed by cognitive impairment. Objective: developing a primer for easy application to the elderly with cognitive impairment. Results: This booklet is a possibility and an option of some important procedures that must be met in relation to the care of older people with cognitive impairment. Conclusion: this material has no pretensions to be anything definitive, but more one of the first to present a concern with the procedures necessary for the care provided to the elderly with cognitive impairment. It is hoped that encourage the academic community to produce instructional material as well as developing more research regarding the care and procedures geared to older adults with cognitive impairment. Keywords: Cognitive deficit; Elderly; Care.  figshare DOI: 10.6084/m9.figshare.978861