Diffusion Enhancement in Core-softened fluid confined in nanotubes

We study the effect of confinement in the dynamical behavior of a core-softened fluid. The fluid is modeled as a two length scales potential. This potential in the bulk reproduces the anomalous behavior observed in the density and in the diffusion of liquid water. A series of $NpT$ Molecular Dynamics simulations for this two length scales fluid confined in a nanotube were performed. We obtain that the diffusion coefficient increases with the increase of the nanotube radius for wide channels as expected for normal fluids. However, for narrow channels, the confinement shows an enhancement in the diffusion coefficient when the nanotube radius decreases. This behavior, observed for water, is explained in the framework of the two length scales potential.Comment: 17 pages, 8 figures, accept for publication at J. Chem. Phy

Avaliação da vacina Circovac(r) no controle da síndrome da circovirose suína (SCS) em condições de campo no Brasil.

bitstream/item/58046/1/CUsersPiazzonDocuments473.pdfProjeto/Plano de Ação: 03.07.51.900-07

Força dos músculos cérvico-escapulares e a severidade da disfunção temporomandibular em mulheres com cervicalgia mecânica

Introdução: modificações da função muscular cervical têm sido verificadas em pacientes com cervicalgia e DTM. Entretanto, ainda não é conhecida a relação entre a severidade da DTM e a força muscular cervical na presença/ausência de cervicalgia. Objetivo: verificar a prevalência de DTM em mulheres com e sem cervicalgia mecânica, avaliar a força dos músculos cérvico-escapulares e sua associação com a severidade da DTM. Métodos: participaram 15 voluntárias sem dor cervical (GC) e 14 mulheres com cervicalgia mecânica (GCM), selecionadas por meio do Índice de Disfunção Relacionada ao Pescoço. O diagnóstico e a gravidade da DTM foram determinados pelos Critérios diagnósticos para pesquisa em desordens temporomandibulares e Índice Temporomandibular (IT), respectivamente. A força dos músculos trapézio superior, flexores e extensores cervicais foi aferida por dinamometria digital manual. Resultados: 64,5% das mulheres com cervicalgia e 33,3% das sem dor cervical apresentaram diagnóstico de DTM (p = 0,095). O GCM apresentou menor força dos músculos flexores (p = 0,044) e extensores cervicais (p = 0,006) e maior IT (p = 0,038) que o GC. Também foi verificada correlação negativa moderada entre o IT e força dos músculos trapézio superior dominante (p = 0,046, r = -0,547), não dominante (p = 0,007, r = -0,695) e flexores cervicais (p = 0,023, r = -0,606) no GCM. Conclusão: não houve diferença na prevalência de DTM entre mulheres com e sem cervicalgia. Entretanto, mulheres com cervicalgia apresentaram menor força muscular cervical (comparadas às sem cervicalgia) que esteve associada a maior severidade da DTM. Assim, em mulheres com cervicalgia associada à DTM, é recomendável avaliar e abordar a severidade desta disfunção e o comprometimento dos músculos cérvico-escapulares.Introduction: changes in cervical muscle function have been observed in patients with neck pain (NP) and TMD. However, the relationship between TMD severity and neck muscle strength in the presence/absence of NP is unknown. Objective: to determine the prevalence of TMD in women with and without mechanical NP and assess the cervical-scapular muscle strength and its association with TMD severity. Methods: fifteen volunteers without neck pain (CG) and 14 women with mechanical neck pain (NPG) took part and were selected by the Neck Disability Index. The diagnosis and severity of TMD were determined by the Research Diagnostic Criteria for TMD and Temporomandibular Index (TI), respectively. The strength of the upper trapezius muscle, and cervical flexor and extensor muscles was measured by digital hand dynamometer. Results: 64.5% of women with NP and 33.3% without NP were diagnosed with TMD (p = 0.095). The NPG showed lower strength of the cervical flexor (p = 0.044) and extensor (p=0.006) muscles, and higher TI (p = 0.038) than in the CG. It was also verified moderate negative correlation between TI and the strength of dominant (p = 0.046, r = -0.547) and non-dominant (p = 0.007, r = -0.695) upper trapezius, and cervical flexors (p = 0.023, r = -0.606) in the NPG. Conclusion: there was no difference in the prevalence of TMD in women with and without NP. However, women with NP have lower cervical muscle strength - compared to those without NP - which was associated with greater severity of TMD. Thus, in women with NP associated with TMD, it is advisable to assess and address the severity of this dysfunction and identify the cervical-scapular muscles compromise

Otimização das técnicas de PCR e RFLP para o gene tbpA de Haemophilus parasuis.

Projeto/Plano de Ação: 03.07.05.017

Interactional positioning and narrative self-construction in the first session of psychodynamic-interpersonal psychotherapy

The purpose of this study is to identify possible session one indicators of end of treatment psychotherapy outcome using the framework of three types of interactional positioning; client’s self-positioning, client’s positioning between narrated self and different partners, and the positioning between client and therapist. Three successful cases of 8-session psychodynamic-interpersonal (PI) therapy were selected on the basis of client Beck Depression Inventory scores. One unsuccessful case was also selected against which identified patterns could be tested. The successful clients were more descriptive about their problems and demonstrated active rapport-building, while the therapist used positionings expressed by the client in order to explore the positionings developed between them during therapy. The unsuccessful case was characterized by lack of positive self-comment, minimization of agentic self-capacity, and empathy-disrupting narrative confusions. We conclude that the theory of interactional positioning has been useful in identifying patterns worth exploring as early indicators of success in PI therapy

Causas infecciosas de problemas reprodutivos na produção de suínos.

bitstream/item/57975/1/CUsersPiazzonDocuments498.pd

MKP-1 mediates glucocorticoid-induced ERK1/2 dephosphorylation and reduction in pancreatic beta-cell proliferation in islets from early lactating mothers

Maternal pancreatic islets undergo a robust increase of mass and proliferation during pregnancy, which allows a compensation of gestational insulin resistance. Studies have described that this adaptation switches to a low proliferative status after the delivery. the mechanisms underlying this reversal are unknown, but the action of glucocorticoids (GCs) is believed to play an important role because GCs counteract the pregnancy-like effects of PRL on isolated pancreatic islets maintained in cell culture. Here, we demonstrate that ERK1/2 phosphorylation (phospho-ERK1/2) is increased in maternal rat islets isolated on the 19th day of pregnancy. Phospho-ERK1/2 status on the 3rd day after delivery (L3) rapidly turns to values lower than that found in virgin control rats (CTL). MKP-1, a protein phosphatase able to dephosphorylate ERK1/2, is increased in islets from L3 rats. Chromatin immunoprecipitation assay revealed that binding of glucocorticoid receptor (GR) to MKP-1 promoter is also increased in islets from L3 rats. in addition, dexamethasone (DEX) reduced phospho-ERK1/2 and increased MKP-1 expression in RINm5F and MIN-6 cells. Inhibition of transduction with cycloheximide and inhibition of phosphatases with orthovanadate efficiently blocked DEX-induced downregulation of phospho-ERK1/2. in addition, specific knockdown of MKP-1 with siRNA suppressed the downregulation of phosphoERK1/2 and the reduction of proliferation induced by DEX. Altogether, our results indicate that downregulation of phospho-ERK1/2 is associated with reduction in proliferation found in islets of early lactating mothers. This mechanism is probably mediated by GC-induced MKP-1 expression.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de PesquisaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, SP, BrazilUniv Estadual Campinas, Fac Med Sci, Dept Pharmacol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biol Sci, Diadema, SP, BrazilWeb of Scienc

Mental health in primary care: ways to reach an integral care

A great challenge for the primary healthcare system, implemented by the strategy called the Family Health Program, is to incorporate actions for facing situations of violence and mental health problems. This study analyzed the care delivered to 411 children between five and eleven years of age in a primary care unit in the city of São Paulo. The clinical findings were compared to a standard inventory of symptoms (CBCL). In addition, semi-structured interviews were held with pediatricians. The study shows low capacity of the pediatricians to recognize mental health problems in children. This is mainly due to deficiencies in their training and lack of possibilities for concrete intervention to face a complaint or diagnostic hypothesis. The reorganization process of primary care will need to provide specific technical support in mental health, incorporating more appropriate technologies for intervention such as a humanized approach and qualified listening. The inclusion of psychosocial aspects in the everyday practice of primary care will make it possible to broaden the concept of health and open way for an integrated approach to situations of violence related to children assisted by the primary care network of the Brazilian Health System.A atenção básica de saúde, impulsionada pela estratégia da saúde da família, tem o grande desafio de incorporar de ações de enfrentamento às situações de violência e problemas de saúde mental. O trabalho analisou a atenção prestada a 411 crianças de cinco a onze anos em uma UBS na cidade de São Paulo. Os dados clínicos foram comparados com um inventário de sintomas padronizados Child Behavior Checklist (CBCL). Entrevistas semi-estruturadas foram aplicadas complementarmente em pediatras. O trabalho mostra que os pediatras têm baixa capacidade de reconhecer problemas de saúde mental em crianças. Os principais fatores relacionados a este baixo desempenho foram: deficiência na formação, carência de possibilidade de atuação concreta frente à queixa ou hipótese diagnóstica. O processo de reorganização do trabalho na atenção básica deveria contemplar a oferta de apoio técnico específico em saúde mental, propiciando a incorporação de tecnologias de intervenção mais apropriadas, como acolhimento e escuta qualificada. A incorporação dos aspectos psicossociais na prática cotidiana da atenção básica possibilitará a ampliação do conceito saúde-doença e abrirá caminho para a abordagem das situações de violência às crianças atendidas na rede básica de serviços de saúde do SUS