72 research outputs found

    Chimeric spike mRNA vaccines protect against Sarbecovirus challenge in mice

    Get PDF
    The emergence of SARS-CoV in 2003 and SARS-CoV-2 in 2019 highlights the need to develop universal vaccination strategies against the broader Sarbecovirus subgenus. Using chimeric spike designs, we demonstrate protection against challenge from SARS-CoV, SARS-CoV-2, SARS-CoV-2 B.1.351, bat CoV (Bt-CoV) RsSHC014, and a heterologous Bt-CoV WIV-1 in vulnerable aged mice. Chimeric spike mRNAs induced high levels of broadly protective neutralizing antibodies against high-risk Sarbecoviruses. In contrast, SARS-CoV-2 mRNA vaccination not only showed a marked reduction in neutralizing titers against heterologous Sarbecoviruses, but SARS-CoV and WIV-1 challenge in mice resulted in breakthrough infections. Chimeric spike mRNA vaccines efficiently neutralized D614G, mink cluster five, and the UK B.1.1.7., and South African B.1.351 variants of concern. Thus, multiplexed-chimeric spikes can prevent SARS-like zoonotic coronavirus infections with pandemic potential

    5th ESO-ESMO international consensus guidelines for advanced breast cancer (ABC 5)

    Get PDF
    The ESO-ESMO 5th international consensus recommendations for advanced breast cancer (ABC 5) provide the latest updates on the management of patients with all breast cancer subtypes, locally advanced breast cancer, follow-up, palliative and supportive care and integrative medicine. Updated diagnostic and treatment algorithms are also provided, as well as several new therapies/indications in advanced breast cancer now approved by the European Medicines Agency

    Nanotechnology advances towards development of targeted-treatment for obesity

    Get PDF
    Obesity through its association with type 2 diabetes (T2D), cancer and cardiovascular diseases (CVDs), poses a serious health threat, as these diseases contribute to high mortality rates. Pharmacotherapy alone or in combination with either lifestyle modifcation or surgery, is reliable in maintaining a healthy body weight, and preventing progression to obesity-induced diseases. However, the anti-obesity drugs are limited by non-specifcity and unsustainable weight loss efects. As such, novel and improved approaches for treatment of obesity are urgently needed. Nanotechnology-based therapies are investigated as an alternative strategy that can treat obesity and be able to overcome the drawbacks associated with conventional therapies. The review presents three nanotechnology-based anti-obesity strategies that target the white adipose tissues (WATs) and its vasculature for the reversal of obesity. These include inhibition of angiogenesis in the WATs, transformation of WATs to brown adipose tissues (BATs), and photothermal lipolysis of WATs. Compared to conventional therapy, the targeted-nanosystems have high tolerability, reduced side efects, and enhanced efcacy. These efects are reproducible using various nanocarriers (liposomes, polymeric and gold nanoparticles), thus providing a proof of concept that targeted nanotherapy can be a feasible strategy that can combat obesity and prevent its comorbiditie

    Vermi-Assisted Bioremediation of Used Engine Oil Contaminated Soil

    No full text
    Effect of earthworm innoculation on vermi-assisted bioremediation of spent engine oil contaminated soil was investigated. The rate of biodegradation of used engine oil was studied for a period of fourteen weeks under laboratory conditions. After 14 weeks, the control sample (without earthworm or organic amendment), the bio-stimulated sample (with organic amendment but without worms) and the earthworm-innoculated sample (with organic amendment and earthworms) recorded 10.08%, 24.33% and 91.92% reduction in pertroleum hydrocarbon content respectively. The co-application of organic amendment and earthworm is recommended for the accelaration of bioremediation of used engine oil contaminated soil.Keywords: cassava peels; used engine oil; bacterial flora; kinetics; Eudriluseugenia
    • …
    corecore