Human RIF1 and protein phosphatase 1 stimulate DNA replication origin licensing but suppress origin activation

We thank David Stead at the Aberdeen Proteomics Service for help in mass spectrometry interpretation, and Raif Yücel and his team at the University of Aberdeen Iain Fraser Cytometry Centre for assistance with flow cytometry. We thank Robert Alver and Julian Blow at University of Dundee for advice on the use of tautomycetin. Peter Cherepanov of the Francis Crick Institute gifted XL413. Daniel Durocher of Lunenfeld-Tanenbaum Research Institute gifted DNA constructs. Work by ADD and SH was supported by Cancer Research UK Grant A13356, Cancer Research UK Programme Award A19059, and BBSRC grant (BB/K006304/1). AIL was supported by Wellcome Trust Awards (108058/Z/15/Z & 105024/Z/14/Z). This work was also supported by JSPS KAKENHI Grant # 16H04739, 25116004 to CO and 16J04327 to YO.Peer reviewedPublisher PD

Role of the DNA damage checkpoint in normal and cancer cell cycle

Katedra genetiky a mikrobiologieDepartment of Genetics and MicrobiologyPřírodovědecká fakultaFaculty of Scienc

Canisterapie in Preschool Setting

The school leaving thesis is elaborated in order to make a connection of canistherapy with the environment of kindergartens. The thesis is devided into two main parts. The theoretical part concerns the development of an individual and the problems of canistherapy and its methods. The practical part reveals a possible connection of canistherapy effects and the environemnt of kindergartens. The aim of the practical part is to find out how the teachers are informed about this term

Role of the DNA damage checkpoint in normal and cancer cell cycle

Katedra genetiky a mikrobiologieDepartment of Genetics and MicrobiologyPřírodovědecká fakultaFaculty of Scienc

Corruption rate and economic performance

This Bachelor´s thesis is focused on a level of corruption and economic influence in selected countries of EU. Theoretical part is focused on a description of the term of corruption, types of corruption, forms of measuring corruption and last but not least causes and consequences of corruption. The basis of the practical part is a regression and correlation analysis which I apply to observed indicators in selected countries of EU. These observed indicators are CPI, GDP in PPP, and Legatum Prosperity Index. The aim of my thesis is to find out if there is a relation among these indicators and then how strong is a dependency among them

Aggression in the workplace

Diplomová práce je zaměřena na agresi na pracovišti, konkrétně na mobbing, bossing, staffing a sexuální obtěžování a její prevenci. Součástí teoretické části je vymezení základních pojmů týkajících se vztahů na pracovišti, a s tím souvisejících konfliktů, agrese a výše uvedených pojmů. Praktická část je založena na výzkumném šetření, které je zacílené na odlišnosti agrese na pracovišti ve státní správě oproti soukromému sektoru.The Diploma Thesis is aimed to the aggression in the workplace, specifically to the mobbing, bossing, staffing, sexual harassment and its prevention. The theoretical part is focused on the definition of basic terms regarding the relations in the workplace, conflicts, aggression and the above mentioned terms. The practical part is based on the questionnaire survey, which is aimed at the aggression differences in the workplace between the state administration and the private sector

Adaptation of music therapists´ practice to COVID-19 pandemic: going virtual; a scoping review protocol

Telemedicin

Adaptation of Music Therapists’ Practice to the Outset of the COVID-19 Pandemic—Going Virtual: A Scoping Review

Background: In the midst of a worldwide COVID-19 pandemic, music therapists previously not involved in telehealth had to develop effective remote forms of music therapy. The objective of this review was to systematically explore how music therapists previously working in-person adapted to the transfer to remote forms of therapy in the context of the coronavirus outbreak. Methods: We searched Scopus, Web of Science Core Collection, CINAHL, Medline, ProQuest Central, PubMed, EMBASE, PsycINFO and PsyARTICLES, grey literature (to October 2020), and websites of professional organizations. We followed the JBI methodology for scoping reviews. Results: Out of the 194 screened texts, we included ten very heterogeneous articles with an overall very low quality. Most texts described remote therapy in the form of synchronous video calls using the Internet, one paper described a concert in a patio of a residential home. We report the authors’ experience with the adaptation and activities, challenges and benefits of remote forms of therapy, recommendations of organizations, and examples and tips for online therapies. Conclusions: Music therapists have adapted the musical instruments, the hours, the technology used, the therapeutic goals, the way they prepared their clients for sessions, and other aspects. They needed to be more flexible, consult with colleagues more often, and mind the client-therapist relationship’s boundaries. It seems, when taken as a necessary short-term measure, online music therapy works sufficiently well. The majority of papers stated that benefits outweighed the challenges, although many benefits were directly linked with the pandemic context

Lipidomické profily plazmy pacientů s karcinomem ledvin, prsu a prostaty se liší od zdravých kontrol

Early detection of cancer is one of the unmet needs in clinical medicine. Peripheral blood analysis is a preferred method for efficient population screening, because blood collection is well embedded in clinical practice and minimally invasive for patients. Lipids are important biomolecules, and variations in lipid concentrations can reflect pathological disorders. Lipidomic profiling of human plasma by the coupling of ultrahigh-performance supercritical fluid chromatography and mass spectrometry is investigated with the aim to distinguish patients with breast, kidney, and prostate cancers from healthy controls. The mean sensitivity, specificity, and accuracy of the lipid profiling approach were 85%, 95%, and 92% for kidney cancer; 91%, 97%, and 94% for breast cancer; and 87%, 95%, and 92% for prostate cancer. No association of statistical models with tumor stage is observed. The statistically most significant lipid species for the differentiation of cancer types studied are CE 16:0, Cer 42:1, LPC 18:2, PC 36:2, PC 36:3, SM 32:1, and SM 41:1 These seven lipids represent a potential biomarker panel for kidney, breast, and prostate cancer screening, but a further verification step in a prospective study has to be performed to verify clinical utility.Včasná detekce rakoviny je jednou z nenaplněných potřeb klinické medicíny. Periferní analýza krve je preferovanou metodou pro efektivní populační screening, protože odběr krve je dobře zakotven v klinické praxi a je minimálně invazivní pro pacienty. Lipidy jsou důležité biomolekuly a odchylky v koncentracích lipidů mohou odrážet patologické poruchy. Lipidomická profilace lidské plazmy spojením ultra-vysokoúčinné superkritické kapalinové chromatografie a hmotnostní spektrometrie je zkoumána s cílem odlišit pacienty s karcinomem prsu, ledvin a prostaty od zdravých kontrol. Průměrná citlivost, specifičnost a přesnost při profilování lipidů byla 85 %, 95 % a 92 % u rakoviny ledvin; 91 %, 97 %, a 94 % pro rakovinu prsu; a 87 %, 95 % a 92 % pro rakovinu prostaty. Není pozorována žádná souvislost statistických modelů se stádiem nádoru. Statisticky nejvýznamnějšími druhy lipidů pro diferenciaci zkoumaných typů nádorů jsou CE 16:0, Cer 42:1, LPC 18:2, PC 36:2, PC 36:3, SM 32:1 a SM 41:1. Těchto sedm lipidů představuje potenciální biomarkerový panel pro screening rakoviny ledvin, prsu a prostaty, ale k ověření klinické užitečnosti musí být proveden další ověřovací krok v prospektivní studii

Phosphorylation-dependent PIH1D1 interactions define substrate specificity of the R2TP cochaperone complex

The R2TP cochaperone complex plays a critical role in the assembly of multisubunit machines, including small nucleolar ribonucleoproteins (snoRNPs), RNA polymerase II, and the mTORC1 and SMG1 kinase complexes, but the molecular basis of substrate recognition remains unclear. Here, we describe a phosphopeptide binding domain (PIH-N) in the PIH1D1 subunit of the R2TP complex that preferentially binds to highly acidic phosphorylated proteins. A cocrystal structure of a PIH-N domain/TEL2 phosphopeptide complex reveals a highly specific phosphopeptide recognition mechanism in which Lys57 and 64 in PIH1D1, along with a conserved DpSDD phosphopeptide motif within TEL2, are essential and sufficient for binding. Proteomic analysis of PIH1D1 interactors identified R2TP complex substrates that are recruited by the PIH-N domain in a sequence-specific and phosphorylation-dependent manner suggestive of a common mechanism of substrate recognition. We propose that protein complexes assembled by the R2TP complex are defined by phosphorylation of a specific motif and recognition by the PIH1D1 subunit