La grossesse ovarienne à propos d’un cas et revue de la littérature

La grossesse ovarienne représente une entité rare parmi les grossesses ectopiques. Son diagnostic et sa prise en charge ne sont pas toujours aisés. C´est une pathologie particulière, le clinicien est confronté à une sémiologie clinique pauvre et à un diagnostic échographique difficile. Les critères chirurgicaux restent difficiles à prouver. Nous avons colligé un cas de grossesse ovarienne. La patiente a consulté nos urgences pour des douleurs pelviennes, métrorragies et une aménorrhée de 9 semaines d’aménorrhée (SA). Le diagnostic préopératoire a été évoqué à l´échographie qui a montré une image latéro utérine droite de 7*8cm au dépend de l'ovaire droit. Une laparotomie a été réalisée en urgence. Le traitement chirurgical a été radical après l´échec du traitement conservateur. La grossesse ovarienne est une entité rare de la grossesse extra-utérine qui présente certaines particularités sémiologiques. Son diagnostic est difficile et se base sur des constatations per-opératoires. Sa prise en charge thérapeutique reste pour le traitement des grossesses extra-utérines, malgré le progrès de traitement médical, chirurgicale

MOLECULAR ANALYSIS OF GYRODINIUM INSTRIATUM EXCYSTMENT CELL USING FTA TECHNOLOGY

Abstract Gyrodinium sp. (BgT3C2) strain isolated from a resting cyst in sediment from Bougrara lagoon (Tunisia) was used to investigate the utility of FTA Technology for the protection and purification of nucleic acid. We have used FTA cards to obtain the sequence of the LSU rDNA D1-D2 region from very low concentrated BgT3C2 strain culture. Nucleotide sequence analysis supported and confirmed identification of BgT3C2 strain to Gyrodinium instriatum. Keywords : Biotechnologies, Genetics, Phytoplankton. Introduction Using molecular approaches revolutionised the identification and taxonomic of the organisms with their genetic characterization, mostly based on nuclear ribosomal DNA marker

Portfolio credit risk models and name concentration issues: theory and simulations

Credit risk is an important aspect in the activity of commercial banks. Regulators require from banks to measure credit risk within Basel II and also during the Internal Capital Assessment and Adequacy Process, ICAAP and stress tests. Name concentration in a lending portfolio arises when there are few borrowers in a bank portfolio or when loan amounts are very unequal in distribution. The portfolio credit risk model underpinning the Basel II Internal Ratings-Based (IRB) approach does not account for name concentration. To measure the latter the literature proposes specific concentration indexes such as the Herfindahl-Hirschman index, the Gini index or more general approaches like the granularity adjustment (GA) to calculate the appropriate economic capital needed to cover the risk arising from the potential default of large borrowers. This paper investigates the practical aspects of granularity adjustment, Gini index and herfindahl-index for contribution\u27s quantification of name concentrations to portfolio risk. We try also to extend the upper bound approach of GA developed by Gordy and Lütkebohmert (2007). For many banks, this approach would permit dramatic reductions in data requirements relative to the full GA

Improvement of Bacillus thuringiensis bioinsecticide production by sporeless and sporulating strains using response surface methodology

Statistical experimental designs, involving a Plackett-Burman design followed by a rotatable central composite design were used to optimize the culture medium constituents for Bacillus thuringiensis bioinsecticide production. This was carried out by using firstly an asporogenic strain and extrapolated to some sporeless and sporulating strains. Initial screening of production parameters was performed and the variables with statistically significant effects on delta-endotoxin production were identified: glucose, glycerol, yeast extract and MnSO(4). These variables were selected for further optimization by response surface methodology. The obtained results revealed that the optimum culture medium for delta-endotoxin production consists of 22.5 g/l of glucose, 4.8g/l of glycerol, 5.8 g/l of yeast extract and 0.008 g/l of MnSO(4). Under these conditions, delta-endotoxin production was 2,130 and 2,260 mg/l into 250 and 1,000 ml flask respectively, which represent more than 38% improvement in toxin production over the basal medium (1,636 mg/l). Such medium composition was shown to be suitable for overproducing delta-endotoxins by sporeless and sporulating strains.Tunisian Ministry of Higher Education, Scientific Research and Technolog

Impact of cigarette butts on microbial diversity and dissolved trace metals in coastal marine sediment

International audience20 21 Cigarette butts are the most common plastic form of litter found in the marine coast, threatening 22 the quality of the seawater and marine life. However, the impact of cigarette butts known to 23 contain toxic chemicals has been investigated to date in very few marine species. This study 24 aimed to evaluate the effects of cigarette filters (smoked or unsmoked) on the microbial 25 diversity inhabiting coastal sediments by high-throughput sequencing of the 16S rRNA genes. 26 Both bacterial structure and metals distribution were impacted by cigarette filter addition in 27 laboratory sediment experiments, compared to control sediment incubations without filter. Both 28 smoked and unsmoked cigarette filters decreased pH and dissolved Cd, Mo and V 29 concentrations in marine sediment incubations, while they increased dissolved Fe, Mn, Zn 30 levels in the surrounding environment. Smoked filters dramatically decreased the relative 31 abundance of the phyla Bacteroidetes and Cyanobacteria, while the members of the phyla 32 Gammaproteobacteria, Firmicutes and Thermotogae were enriched by smoked filters in marine 33 sediments. Bacterial taxa associated with deep marine environments or hydrothermal seep fields 34 were selected by smoked cigarette filters. This study demonstrated for the first time the 35 microbial community changes and impacts from toxic cigarette filters in coastal marine 36 sediments

Picoeukaryotic diversity in the Gulf of Gabès: variability patterns and relationships to nutrients and water masses

17 pages, 7 figures, 3 tables, supplementary material https://doi.org/10.3354/ame01857Marine picoeukaryotes show high phylogenetic diversity worldwide, notably in oligotrophic waters. In the Gulf of Gabès (south-eastern Mediterranean), characterized by oligotrophic conditions and a complex water mass circulation, information on picoeukaryotic diversity is still lacking. In this study, we investigated the diversity and spatial variability of picoeukaryotic assemblages in relation to nutrient availability, physical parameters and water masses in 3 cruises carried out in the Gulf of Gabès in June of 2008, April of 2009 and November of 2009. High-throughput sequencing revealed a dominance of sequences from non photosynthetic picoeukaryotes, mostly represented by the presumably parasitic marine alveolate MALV-II (33.20%) and the bacterivorous Bicosoecida (13.56%). Differences in picoeukaryotic assemblages were higher between coastal and open-sea stations, and depth in the water column also affected community differences, with surface (5 m), intermediate (25-100 m) and mesopelagic (>200 m) samples forming separate groups. A clear temporal variability was also evident, particularly for communities collected from the surface layer and open-sea stations. Co-inertia analysis revealed that picoeukaryotic groups were more affected by salinity in deep waters, whereas at the surface, they were dependent on nutrients and temperature. During the November cruise, samples that shared similar water mass properties generally clustered together. The Levantine water mass, observed for the first time in this area, was characterized by the presence of Acantharia and Polycystinea. Our study highlights the role of physical and chemical features, such as water mass origin, the wide continental shelf and trophic status, in determining the diversity of marine picoeukaryotesThis study was partially supported by the European funded project ‘Improving National Capacities in Observation and Management of Marine Environment in Tunisia’ (INCOMMET, 295009)Peer Reviewe

Ultraphytoplankton community structure in subsurface waters along a North-South Mediterranean transect

International audienc

Ultraphytoplankton community structure in subsurface waters along a North-South Mediterranean transect

International audienc

Combinatorial effect of mutagenesis and medium component optimization on Bacillus amyloliquefaciens antifungal activity and efficacy in eradicating Botrytis cinerea

This work is directed towards Bacillus amyloliquefaciens strain BLB371 metabolite production for biocontrol of fungal phytopathogens. In order to maximise antifungal metabolite production by this strain, two approaches were combined: random mutagenesis and medium component optimization. After three rounds of mutagenesis, a hyper active mutant, named M3-7, was obtained. It produces 7 fold more antifungal metabolites (1800 AU/mL) than the wild strain in MC medium. A hybrid design was applied to optimise a new medium to enhance antifungal metabolite production by M3-7. The new optimized medium (35 g/L of peptone, 32.5 g/L of sucrose, 10.5 g/L of yeast extract, 2.4 g/L of KH2PO4, 1.3 g/L of MgSO4 and 23 mg/L of MnSO4) achieved 1.62 fold enhancement in antifungal compound production (3000 AU/mL) by this mutant, compared to that achieved in MC medium. Therefore, combinatory effect of these two approaches (mutagenesis and medium component optimization) allowed 12 fold improvement in antifungal activity (from 250 UA/mL to 3000 UA/mL). This improvement was confirmed against several phytopathogenic fungi with an increase of MIC and MFC over than 50%. More interestingly, a total eradication of gray mold was obtained on tomato fruits infected by Botrytis cinerea and treated by M3-7, compared to those treated by BLB371. From the practical point of view, combining random mutagenesis and medium optimization could be considered as an excellent tool for obtaining promising biological products useful against phytopathogenic fungi.Scopu