Effect of Eight Weeks of Resistance Training with Limitation of Blood Flowing on Insulin-1 Factor, Agerin C Cerpinal Perpetrate and Elderly Composition

سابقه و هدف: تغییرات در دوره سالمندی باعث ایجاد برخی مشکلات در افراد سالمند می‌شود که این تغییرات می‌تواند آناتومیکی یا فیزیولوژیکی باشد. هدف‌پژوهش حاضرتاثیر هشت‌هفته تمرینات مقاومتی با و بدون محدودیت‌جریان خون بر فاکتورشبه‌انسولین-1، پپتید قطعه اگرین‌C ترمینال و ترکیب بدن سالمندان بود. روش‌ بررسی:روش پژوهش‌حاضر نیمه‌تجربی وباطرح پیش‌آزمون- پس‌آزمون بود. جامعه آماری دراين تحقيق، مردان شهرمشهد بودند که براساس معیارهای ورودبه تحقیق پس از غربالگری اولیه، تعداد 45 نفر به صورت تصادفی با دامنه سنی 83/2 ±11/64‌(سال) و قد 36/5 ± 44/173(سانتیمتر)، وزن 99/5  ± 34/81(کیلوگرم)، شاخص توده‌بدنی 51/1 ± 03/27، توده‌جربی 26/2  ± 68/26 (درصد) وتوده عضلانی 07/4 ± 36/53  درسه گروه‌تمرین مقاومتی با(15نفر) و بدون(15نفر) محدودیت‌ جریان ‌خون و گروه کنترل (15نفر) تقسیم شدند. داده‌ها بااستفاده ازآزمون تحلیل واریانس یک‌طرفه وآزمون‌تعقیبی‌توکی در‌سطح معنی‌داری 05/0≥P ونرم افزار SPSS نسخه 21 تحلیل شدند. یافته ها: نتایج پژوهش حاضر نشان داد میزان سرمی IGF-1 افزایش معنی داری بین‌گروه‌های تمرین مقاومتی با و بدون محدودیت جریان خون نسبت به گروه کنترل داشت (001/0=P، 02/49=F). همچنین تمرین مقاومتی بامحدودیت جریان‌خون تاثیربیشتری بر افزایش سطوح 1-IGF(41/253=ES ، 61/22=PC) نسبت به‌گروه تمرین مقاومتی بدون محدودیت جریان خون داشت(200=ES ، 31/1=PC). نتایج همچنین نشان داد میزان سرمی پپتیدC بین گروه‌های تمرین مقاومتی باوبودن محدودیت جریان خون با گروه کنترل کاهش معناداری وجودداشت (001/0=P ، 93/39=F) اما بین گروه‌های تجربی تفاوتی معنی‌داری وجود نداشت (493/0=F، 09/0=P). نتایج همچنین نشان داد تمرین با و بدون محدودیت جریان خون سبب کاهش توده چربی ( 001/0=P، 95/16=F) و شاخص توده بدن (001/0=P، 30/44=F) نسبت به گروه کنترل شد اما بین گروه‌های تجربی تفاوتی وجود نداشت (00/1=P، 46/0=F). نتیجه گیري: تمرینات مقاومتی با و بدون محدودیت جریان‌خون سبب افزایش1- IGF و کاهش پپتید CAF، توده چربی و شاخص توده بدن سالمندان می‌شود. How to cite this article: Zoraqi MR, Barjaste Yazdi A, Khajei R, Rashidlamir A. Effect of Eight Weeks of Resistance Training with Limitation of Blood Flowing on Insulin-1 Factor, Agerin C Cerpinal Perpetrate and Elderly Composition. Irtiqa Imini Pishgiri Masdumiyat. 2021;9(2):153-64.Background and Objectives: Changes in old age cause some problems in the elderly that these changes can be anatomical or physiological. The aim of the present study was to evaluate the effect of eight weeks of resistance training without blood flow on insulin-like factor (IGF-1), C-terminal agrin fragment peptide (CAF) and body composition of the elderly. Materials and Methods: The present research method is quasi-experimental and pre-test-post-test design. The statistical population in this study is the men of Mashhad. According to the inclusion criteria, after initial screening by the researcher, 45 people were randomly selected. ), Weight 81.34 ±5.99 (kg), body mass index 27.03 ± 1.51, experimental mass 26.26 ± 2.26 (percent) and muscle mass 53.36 ±4.07 in three groups Resistance training with (15 people) and without (15 people) restriction of blood flow و and control group (15 people) were divided. Data were analyzed using one-way analysis of variance and Tukey test at the significance level of P /0.05 and SPSS software version 21. Results: The results of the present study showed that the serum level of IGF-1 had a significant increase between the groups of resistance training with and without restriction of blood flow compared to the control group (P = 0.001, F = 49.02). Also, resistance training with limited blood flow had a greater effect on increasing IGF-1 levels (ES = 352.14, PC = 22.61) than the resistance training group without blood flow restriction (ES = 200, PC = 1.31). The results also showed that there was a significant decrease in serum C-peptide level between resistance training groups with limited blood flow and control group (P = 0.001, F = 39.93) but there was no significant difference between experimental groups (493 / 0 = F, 09/0 = P). The results also showed that exercise with and without restriction of blood flow reduced fat mass (P = 0.001, F = 16.95) and body mass index (P = 0.001, F = 44.30) compared to the control group. But there was no difference between the experimental groups (P = 1.00, F = 0.46). Conclusion: Resistance training without blood flow restriction increases IGF-1 and decreases CAF peptide, fat mass and body mass index in the elderly. How to cite this article: Zoraqi MR, Barjaste Yazdi A, Khajei R, Rashidlamir A. Effect of Eight Weeks of Resistance Training with Limitation of Blood Flowing on Insulin-1 Factor, Agerin C Cerpinal Perpetrate and Elderly Composition. Irtiqa Imini Pishgiri Masdumiyat. 2021;9(2):153-64. &nbsp

Production of Transgenic Pigs Mediated by Pseudotyped Lentivirus and Sperm

Sperm-mediated gene transfer can be a very efficient method to produce transgenic pigs, however, the results from different laboratories had not been widely repeated. Genomic integration of transgene by injection of pseudotyped lentivirus to the perivitelline space has been proved to be a reliable route to generate transgenic animals. To test whether transgene in the lentivirus can be delivered by sperm, we studied incubation of pseudotyped lentiviruses and sperm before insemination. After incubation with pig spermatozoa, 62±3 lentiviral particles were detected per 100 sperm cells using quantitative real-time RT-PCR. The association of lentivirus with sperm was further confirmed by electron microscopy. The sperm incubated with lentiviral particles were artificially inseminated into pigs. Of the 59 piglets born from inseminated 5 sows, 6 piglets (10.17%) carried the transgene based on the PCR identification. Foreign gene and EGFP was successfully detected in ear tissue biopsies from two PCR-positive pigs, revealed via in situ hybridization and immunohistochemistry. Offspring of one PCR-positive boar with normal sows showed PCR-positive. Two PCR-positive founders and offsprings of PCR-positive boar were further identified by Southern-blot analysis, out of which the two founders and two offsprings were positive in Southern blotting, strongly indicating integration of foreign gene into genome. The results indicate that incubation of sperm with pseudotyped lentiviruses can incorporated with sperm-mediated gene transfer to produce transgenic pigs with improved efficiency

Genomic analyses inform on migration events during the peopling of Eurasia.

High-coverage whole-genome sequence studies have so far focused on a limited number of geographically restricted populations, or been targeted at specific diseases, such as cancer. Nevertheless, the availability of high-resolution genomic data has led to the development of new methodologies for inferring population history and refuelled the debate on the mutation rate in humans. Here we present the Estonian Biocentre Human Genome Diversity Panel (EGDP), a dataset of 483 high-coverage human genomes from 148 populations worldwide, including 379 new genomes from 125 populations, which we group into diversity and selection sets. We analyse this dataset to refine estimates of continent-wide patterns of heterozygosity, long- and short-distance gene flow, archaic admixture, and changes in effective population size through time as well as for signals of positive or balancing selection. We find a genetic signature in present-day Papuans that suggests that at least 2% of their genome originates from an early and largely extinct expansion of anatomically modern humans (AMHs) out of Africa. Together with evidence from the western Asian fossil record, and admixture between AMHs and Neanderthals predating the main Eurasian expansion, our results contribute to the mounting evidence for the presence of AMHs out of Africa earlier than 75,000 years ago.Support was provided by: Estonian Research Infrastructure Roadmap grant no 3.2.0304.11-0312; Australian Research Council Discovery grants (DP110102635 and DP140101405) (D.M.L., M.W. and E.W.); Danish National Research Foundation; the Lundbeck Foundation and KU2016 (E.W.); ERC Starting Investigator grant (FP7 - 261213) (T.K.); Estonian Research Council grant PUT766 (G.C. and M.K.); EU European Regional Development Fund through the Centre of Excellence in Genomics to Estonian Biocentre (R.V.; M.Me. and A.Me.), and Centre of Excellence for Genomics and Translational Medicine Project No. 2014-2020.4.01.15-0012 to EGC of UT (A.Me.) and EBC (M.Me.); Estonian Institutional Research grant IUT24-1 (L.S., M.J., A.K., B.Y., K.T., C.B.M., Le.S., H.Sa., S.L., D.M.B., E.M., R.V., G.H., M.K., G.C., T.K. and M.Me.) and IUT20-60 (A.Me.); French Ministry of Foreign and European Affairs and French ANR grant number ANR-14-CE31-0013-01 (F.-X.R.); Gates Cambridge Trust Funding (E.J.); ICG SB RAS (No. VI.58.1.1) (D.V.L.); Leverhulme Programme grant no. RP2011-R-045 (A.B.M., P.G. and M.G.T.); Ministry of Education and Science of Russia; Project 6.656.2014/K (S.A.F.); NEFREX grant funded by the European Union (People Marie Curie Actions; International Research Staff Exchange Scheme; call FP7-PEOPLE-2012-IRSES-number 318979) (M.Me., G.H. and M.K.); NIH grants 5DP1ES022577 05, 1R01DK104339-01, and 1R01GM113657-01 (S.Tis.); Russian Foundation for Basic Research (grant N 14-06-00180a) (M.G.); Russian Foundation for Basic Research; grant 16-04-00890 (O.B. and E.B); Russian Science Foundation grant 14-14-00827 (O.B.); The Russian Foundation for Basic Research (14-04-00725-a), The Russian Humanitarian Scientific Foundation (13-11-02014) and the Program of the Basic Research of the RAS Presidium “Biological diversity” (E.K.K.); Wellcome Trust and Royal Society grant WT104125AIA & the Bristol Advanced Computing Research Centre (http://www.bris.ac.uk/acrc/) (D.J.L.); Wellcome Trust grant 098051 (Q.A.; C.T.-S. and Y.X.); Wellcome Trust Senior Research Fellowship grant 100719/Z/12/Z (M.G.T.); Young Explorers Grant from the National Geographic Society (8900-11) (C.A.E.); ERC Consolidator Grant 647787 ‘LocalAdaptatio’ (A.Ma.); Program of the RAS Presidium “Basic research for the development of the Russian Arctic” (B.M.); Russian Foundation for Basic Research grant 16-06-00303 (E.B.); a Rutherford Fellowship (RDF-10-MAU-001) from the Royal Society of New Zealand (M.P.C.)

First Insight into Genetic Diversity of the Mycobacterium tuberculosis Complex in Albania Obtained by Multilocus Variable-Number Tandem-Repeat Analysis and Spoligotyping Reveals the Presence of Beijing Multidrug-Resistant Isolates ▿

We characterized a set of 100 Mycobacterium tuberculosis complex clinical isolates from tuberculosis (TB) patients in Albania, typing them with a 24-locus variable-number tandem-repeat-spoligotyping scheme. Depending on the cluster definition, 43 to 49 patients were distributed into 15 to 16 clusters which were likely to be epidemiologically linked, indicative of a recent transmission rate of 28 to 34%. This result suggests that TB is under control in Albania. However, two multidrug-resistant (MDR) Beijing genotypes harboring the same S531A mutation on the rpoB gene were also found, suggesting a potential recent transmission of MDR TB. Three brand new genotypes, Albania-1 to Albania-3, are also described

Sperm-mediated gene transfer: Production of pigs transgenic for a human regulator of complement activation

[No abstract available