Texture segmentation as first step towards archaeological object detection in high-resolution satellite images of the Silvretta Alps

Since 2007, the Silvretta Archaeological Project in the high Alps on the Swiss-Austrian border has been investigating the prehistoric origins of alpine pasture economy. In an area of about 540 km2 more than 20 well-preserved archaeological sites associated with alpine pastoralism have been recorded, the earliest of them dating to the Iron Age (Reitmaier (ed.), 2012; Walser and Lambers, 2012). All of the ruined huts, cellars and livestock enclosures at these sites are visible on the surface and show a limited range of shapes and proportions. According to their function, all of them are located in open grassland. Based on this sample, we are currently developing methods to detect archaeological objects of the kind described above in high-resolution satellite images of our study area (Lambers and Zingman, in press). These methods are intended to assist archaeological survey in vast and/or difficult to access areas by screening large amounts of remotely sensed images in order to detect possible archaeological sites prior to fieldwork (Cowley, 2012). Our general approach aims at assessing the probability of the presence of objects of our interest based on geometric cues that can be automatically detected in the satellite and aerial images that we use. We here describe our general methodology and the first integral step constituting a new approach to texture segmentation.Digital Archaeolog

Detection of incomplete enclosures of rectangular shape in remotely sensed images

We develop an approach for detection of ruins of livestock enclosures in alpine areas captured by high-resolution remotely sensed images. These structures are usually of approximately rectangular shape and appear in images as faint fragmented contours in complex background. We address this problem by introducing a new rectangularity feature that quantifies the degree of alignment of an optimal subset of extracted linear segments with a contour of rectangular shape. The rectangularity feature has high values not only for perfect enclosures, but also for broken ones with distorted angles, fragmented walls, or even a completely missing wall. However, it has zero value for spurious structures with less than three sides of a perceivable rectangle. Performance analysis using large imagery of an alpine environment is provided. We show how the detection performance can be improved by learning from only a few representative examples and a large number of negatives.Computer SciencesEuropean Prehistor

Detection of fragmented rectangular enclosures in very high resolution remote sensing images

We develop an approach for the detection of ruins of livestock enclosures (LEs) in alpine areas captured by high-resolution remotely sensed images. These structures are usually of approximately rectangular shape and appear in images as faint fragmented contours in complex background. We address this problem by introducing a rectangularity feature that quantifies the degree of alignment of an optimal subset of extracted linear segments with a contour of rectangular shape. The rectangularity feature has high values not only for perfectly regular enclosures but also for ruined ones with distorted angles, fragmented walls, or even a completely missing wall. Furthermore, it has a zero value for spurious structures with less than three sides of a perceivable rectangle. We show how the detection performance can be improved by learning a linear combination of the rectangularity and size features from just a few available representative examples and a large number of negatives. Our approach allowed detection of enclosures in the Silvretta Alps that were previously unknown. A comparative performance analysis is provided. Among other features, our comparison includes the state-of-the-art features that were generated by pretrained deep convolutional neural networks (CNNs). The deep CNN features, although learned from a very different type of images, provided the basic ability to capture the visual concept of the LEs. However, our handcrafted rectangularity-size features showed considerably higher performance.European Prehistor

Statistical Analysis of Readthrough Levels for Nonsense Mutations in Mammalian Cells Reveals a Major Determinant of Response to Gentamicin

The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for diseases caused by premature termination codons. However, the readthrough response to treatment is highly variable and little is known about the rules governing readthrough level and response to aminoglycosides. In this study, we carried out in-depth statistical analysis on a very large set of nonsense mutations to decipher the elements of nucleotide context responsible for modulating readthrough levels and gentamicin response. We quantified readthrough for 66 sequences containing a stop codon, in the presence and absence of gentamicin, in cultured mammalian cells. We demonstrated that the efficiency of readthrough after treatment is determined by the complex interplay between the stop codon and a larger sequence context. There was a strong positive correlation between basal and induced readthrough levels, and a weak negative correlation between basal readthrough level and gentamicin response (i.e. the factor of increase from basal to induced readthrough levels). The identity of the stop codon did not affect the response to gentamicin treatment. In agreement with a previous report, we confirm that the presence of a cytosine in +4 position promotes higher basal and gentamicin-induced readthrough than other nucleotides. We highlight for the first time that the presence of a uracil residue immediately upstream from the stop codon is a major determinant of the response to gentamicin. Moreover, this effect was mediated by the nucleotide itself, rather than by the amino-acid or tRNA corresponding to the −1 codon. Finally, we point out that a uracil at this position associated with a cytosine at +4 results in an optimal gentamicin-induced readthrough, which is the therapeutically relevant variable

Readthrough of Premature Termination Codons in the Adenomatous Polyposis Coli Gene Restores Its Biological Activity in Human Cancer Cells

The APC tumor suppressor gene is frequently mutated in human colorectal cancer, with nonsense mutations accounting for 30% of all mutations in this gene. Reintroduction of the WT APC gene into cancer cells generally reduces tumorigenicity or induces apoptosis. In this study, we explored the possibility of using drugs to induce premature termination codon (PTC) readthrough (aminoglycosides, negamycin), as a means of reactivating endogenous APC. By quantifying the readthrough of 11 nonsense mutations in APC, we were able to identify those giving the highest levels of readthrough after treatment. For these mutations, we demonstrated that aminoglycoside or negamycin treatment led to a recovery of the biological activity of APC in cancer cell lines, and showed that the level of APC activity was proportional to the level of induced readthrough. These findings show that treatment with readthrough inducers should be considered as a potential strategy for treating cancers caused by nonsense mutations APC gene. They also provide a rational basis for identifying mutations responsive to readthrough inducers

A Kir6.2 mutation causing severe functional effects in vitro produces neonatal diabetes without the expected neurological complications

AIMS/HYPOTHESIS: Heterozygous activating mutations in the pancreatic ATP-sensitive K+ channel cause permanent neonatal diabetes mellitus (PNDM). This results from a decrease in the ability of ATP to close the channel, which thereby suppresses insulin secretion. PNDM mutations that cause a severe reduction in ATP inhibition may produce additional symptoms such as developmental delay and epilepsy. We identified a heterozygous mutation (L164P) in the pore-forming (Kir6.2) subunit of the channel in three unrelated patients and examined its functional effects. METHODS: The patients (currently aged 2, 8 and 20 years) developed diabetes shortly after birth. The two younger patients attempted transfer to sulfonylurea therapy but were unsuccessful (up to 1.1 mg kg(-1) day(-1)). They remain insulin dependent. None of the patients displayed neurological symptoms. Functional properties of wild-type and mutant channels were examined by electrophysiology in Xenopus oocytes. RESULTS: Heterozygous (het) and homozygous L164P K(ATP) channels showed a marked reduction in channel inhibition by ATP. Consistent with its predicted location within the pore, L164P enhanced the channel open state, which explains the reduction in ATP sensitivity. HetL164P currents exhibited greatly increased whole-cell currents that were unaffected by sulfonylureas. This explains the inability of sulfonylureas to ameliorate the diabetes of affected patients. CONCLUSIONS/INTERPRETATION: Our results provide the first demonstration that mutations such as L164P, which produce a severe reduction in ATP sensitivity, do not inevitably cause developmental delay or neurological problems. However, the neonatal diabetes of these patients is unresponsive to sulfonylurea therapy. Functional analysis of PNDM mutations can predict the sulfonylurea response

Slaget om privatannonsmarknaden GP

Pattern spectra have frequently been used in image analysis. A drawback is that they are not sensitive to changes in spatial distribution of features. Various methods have been proposed to address this problem. In this paper we compare several of these on both texture classification and image retrieval. Results show that Size Density Spectra are most versatile, and least sensitive to parameter settings

Relativistic nuclear fluid dynamics and VUU kinetic theory

Relativistic kinetic theory may be used to understand hot dense hadronic matter. We address the questions of collective flow and pion production in a 3 D relativistic fluid dynamic model and in the VUU microscopic theory. The GSI/LBL collective flow and pion data point to a stiff equation of state. The effect of the nuclear equation of state on the thermodynamic parameters is discussed. The properties of dense hot hadronic matter are studied in Au + Au collisions from 0.1 to 10 GeV/nucleon. 22 refs., 5 figs

613 cases of splenic rupture without risk factors or previously diagnosed disease: a systematic review

Background Rupture of the spleen in the absence of trauma or previously diagnosed disease is largely ignored in the emergency literature and is often not documented as such in journals from other fields. We have conducted a systematic review of the literature to highlight the surprisingly frequent occurrence of this phenomenon and to document the diversity of diseases that can present in this fashion. Methods Systematic review of English and French language publications catalogued in Pubmed, Embase and CINAHL between 1950 and 2011. Results We found 613 cases of splenic rupture meeting the criteria above, 327 of which occurred as the presenting complaint of an underlying disease and 112 of which occurred following a medical procedure. Rupture appeared to occur spontaneously in histologically normal (but not necessarily normal size) spleens in 35 cases and after minor trauma in 23 cases. Medications were implicated in 47 cases, a splenic or adjacent anatomical abnormality in 31 cases and pregnancy or its complications in 38 cases. The most common associated diseases were infectious (n = 143), haematologic (n = 84) and non-haematologic neoplasms (n = 48). Amyloidosis (n = 24), internal trauma such as cough or vomiting (n = 17) and rheumatologic diseases (n = 10) are less frequently reported. Colonoscopy (n = 87) was the procedure reported most frequently as a cause of rupture. The anatomic abnormalities associated with rupture include splenic cysts (n = 6), infarction (n = 6) and hamartomata (n = 5). Medications associated with rupture include anticoagulants (n = 21), thrombolytics (n = 13) and recombinant G-CSF (n = 10). Other causes or associations reported very infrequently include other endoscopy, pulmonary, cardiac or abdominal surgery, hysterectomy, peliosis, empyema, remote pancreato-renal transplant, thrombosed splenic vein, hemangiomata, pancreatic pseudocysts, splenic artery aneurysm, cholesterol embolism, splenic granuloma, congenital diaphragmatic hernia, rib exostosis, pancreatitis, Gaucher's disease, Wilson's disease, pheochromocytoma, afibrinogenemia and ruptured ectopic pregnancy. Conclusions Emergency physicians should be attuned to the fact that rupture of the spleen can occur in the absence of major trauma or previously diagnosed splenic disease. The occurrence of such a rupture is likely to be the manifesting complaint of an underlying disease. Furthermore, colonoscopy should be more widely documented as a cause of splenic rupture