Ubiquitination is indispensible for the insulin-sensitising activity of the adaptor protein APPL1

published_or_final_versio

Highly sensitive and selective biosensor based on graphene oxide coated long period grating

We propose an optical fiber immunosensor based on graphene oxide coated dual-peak long period grating (GO-dLPG), in which GO-IgG linking layer is used for rapid immunoassays. The binding interaction between antibody and antigen produced a detectable optical signal in terms of grating resonant wavelength shift, which was proportional to the analyte concentration. By deposition of GO overlay, the bulk RI sensitivity of dLPG was enhanced around 150%. The GO-coated dLPG was biofunctionalized by the immobilization of IgG to generate the biosensor. The IgG-bound GO-dLPG was used to detect the anti-IgG and anti-PSA, respectively, demonstrating high sensitivity and selectivity. The GO-dLPG biosensor can be further developed as a biosensing platform with advantages of label-free, real-time and low limit of detection

Simple models for rope substructure mechanics: Application to electro-mechanical lifts

Mechanical systems modelled as rigid mass elements connected by tensioned slender structural members such as ropes and cables represent quite common substructures used in lift engineering and hoisting applications. Special interest is devoted by engineers and researchers to the vibratory response of such systems for optimum performance and durability. This paper presents simplified models that can be employed to determine the natural frequencies of systems having substructures of two rigid masses constrained by tensioned rope/cable elements. The exact solution for free un-damped longitudinal displacement response is discussed in the context of simple two-degree-of-freedom models. The results are compared and the influence of characteristics parameters such as the ratio of the average mass of the two rigid masses with respect to the rope mass and the deviation ratio of the two rigid masses with respect to the average mass is analyzed. This analysis gives criteria for the application of such simplified models in complex elevator and hoisting system configurations

Resistance of Dictyostelium discoideum membranes to saponin permeabilization

<p>Abstract</p> <p>Background</p> <p>Saponin is a mild detergent commonly used to permeabilize cells prior to immunofluorescence labeling of intracellular proteins. It has previously been used to that effect in <it>Dictyostelium discoideum </it>amoebae.</p> <p>Findings</p> <p>We show that saponin, contrary to Triton X-100 or alcohol, permeabilizes at best incompletely membranes of <it>Dictyostelium</it>. In cells exposed to osmotic stress, almost complete resistance to saponin permeabilization was observed.</p> <p>Conclusions</p> <p>Saponin should be used with special care to permeabilize <it>Dictyostelium </it>membranes. This unsusual property is presumably linked to the specific sterol composition of <it>Dictyostelium </it>membranes. It may also represent an adaptation of <it>Dictyostelium </it>to harsh conditions or to natural compounds encountered in its natural environment.</p

An allocation Malmquist index with an application in the China securities industry

This paper proposes an allocation Malmquist index which is inspired by the work on the non-parametric cost Malmquist index. We first show that how to decompose the cost Malmquist index into the input-oriented Malmquist index and the allocation Malmquist index. An application in corporate management of the China securities industry with the panel data set of 40 securities companies during the period 2005–2011 shows the practicality of the propose model

A QM/MM approach for the study of monolayer-protected gold clusters

We report the development and implementation of hybrid methods that combine quantum mechanics (QM) with molecular mechanics (MM) to theoretically characterize thiolated gold clusters. We use, as training systems, structures such as Au25(SCH2-R)18 and Au38(SCH2-R)24, which can be readily compared with recent crystallographic data. We envision that such an approach will lead to an accurate description of key structural and electronic signatures at a fraction of the cost of a full quantum chemical treatment. As an example, we demonstrate that calculations of the 1H and 13C NMR shielding constants with our proposed QM/MM model maintain the qualitative features of a full DFT calculation, with an order-of-magnitude increase in computational efficiency.Comment: Journal of Materials Science, 201

Editorial: Integrative Physiology of Common Chronic Musculoskeletal Disorders

This is the final version. Available on open access from Frontiers media via the DOI in this recor