Molecular detection of Torque teno virus in different breeds of swine

<p>Abstract</p> <p>Background</p> <p>Torque teno virus (TTV), of the <it>Anelloviridae </it>family, <it>Iotatorquevirus </it>genus, is a non-enveloped, single-stranded, and negative sense DNA (ssDNA) virus infecting human and many domestic animals including swines. Very little information is known about the investigations of TTV prevalence in different swine breeds so far.</p> <p>Methods</p> <p>In this study, 208 serum samples collected from seven swine breeds (<it>Rongchang pig</it>, <it>Chenghua pig</it>, <it>Zibet pig</it>, <it>Wild boar</it>, <it>Duroc</it>, <it>Landrace</it>, <it>Large Yorkshire</it>) from two independent farms were detected to determine the prevalence of two swine TTV genogroups, TTV1 and TTV 2, by nested polymerase chain reaction methods, and to analyse prevalence difference among these breeds.</p> <p>Results</p> <p>The results showed that the prevalence of TTV in the seven breeds was 92%-100%. No significant difference (p > 0.05) in TTV infection was observed between different breeds. Interestingly, significantly higher prevalence for TTV1 in <it>Rongchang </it>boars (90%) and for TTV2 in <it>Rongchang </it>sows (95%) were detected, while co-infection rate (43.8%) was lower than other breeds. Sequence analysis showed that the homology of TTV1 and TTV2 were over 90.9% and 86.4% in these breeds, respectively.</p> <p>Conclusions</p> <p>The results indicated that TTV was widely distributed in the seven swine breeds. The prevalence of both TTV genogroups associated with swine breeds and genders. This study also respented the first description of swine TTV prevalence in different swine breeds. It was vitally necessary to further study swine TTV pathogenicity.</p

Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses?

Periductal mastitis (PDM) is a prolonged inflammatory disease, but the cause of PDM is poorly understood. In the present case control study, 87 PDM and 87 healthy controls were enrolled and the results were evaluated to identify the significant risk factors for PDM. To investigate the roles of bacterial infection and critical cytokines expression, 16S rRNA gene sequencing and bacterial culturing were conducted. We also measured the levels of interferon-γ, interleukin-12A, and interleukin-17A by semiquantitative immunohistochemistry method. In a multivariable logistic regression model, we identified overweight/obesity and late onset of menarche as independent risk factors for PDM. In contrast, age of first birth >27 years had a protective effect. With 16S rRNA gene sequencing, we confirmed bacterial infections were found in all PDM patients, but none of the control patients was positive on the gene expression of 16S rRNA. Our results also demonstrated significant increases of the IFN-γ and IL-12A expression in PDM, but there was no difference in IL-17A expression in these two groups. Taken together, this study suggests that reproductive factors and overweight/obesity are possible predisposing risk factors for PDM. Bacterial infection and the increased expression of some proinflammatory cytokines are associated with the pathogenesis of this disease

Self-provided microbial electricity enhanced wastewater treatment using carbon felt anode coated with amino-functionalized Fe3O4

International audienceBioelectricity can be produced from wastewater using microbial fuel cells (MFCs) that produce electricity during electrochemical and biochemical reactions, yet actual applications of wastewater MFCs are limited; a selfprovided microbial electricity enhanced wastewater treatment method is proposed producing microbial power and enhanced pollutants removal simultaneously. A carbon felt coated with amino-functionalized Fe3O4 particles was synthesized; power generation, removal of chemical oxygen demand (COD) and NH4+-N were evaluated with cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), cell voltage and treatment efficiency. Results showed that the amino-Fe3O4 coated anode led to higher NH4+-N removal efficiency of 97.7 % than the Fe3O4 coated anode of 48.9 %. Moreover, the maximum power density of the amino-Fe3O4 anode is 208.67 mW/ m 2 , 35.1 % higher than that of the Fe3O4 anode. This finding was explained by the presence of protonated amino groups NH4+ that favored the attachment of negatively charged bacteria. Amino-functionalization of the anode promotes a promising, novel technology to treat wastewater while producing electricity

Two-dimensional numerical study of gap resonance coupling with motions of floating body moored close to a bottom-mounted wall

The piston mode fluid resonance in the narrow gap between a moored floating body and a bottom-mounted vertical wall is numerically investigated based on a two-dimensional potential flow model and viscous numerical simulations. This study focuses on understanding the effect of mooring stiffness on the coupling dynamics of the gap resonance and the sway or heave motion of the floating body in regular waves. Numerical studies show that the resonant wave amplitude in the gap is reduced by the sway and heave motions. The reduction is highly dependent on the mooring stiffness. Two resonant frequencies are confirmed, and both increase with the mooring stiffness. Different modes of motions are identified in terms of the phase difference between the oscillatory motions of the gap flow and the floating body. Higher harmonic components of responses are found for the specific mooring stiffness. The performance of potential flow models in predicting resonant responses is revisited based on the understanding that the overall damping effect consists of two parts: (1) radiation damping and (2) viscous dissipation. It is confirmed that a potential model is also able to produce reasonable predictions as radiation damping plays a dominant role, for example, at the second resonant frequencies of coupling the gap resonance with the sway motion. Otherwise, as viscous dissipation dominates radiation damping, noticeable over-predictions by a potential model occur as recognized before, for example, the present results at the second peak response of gap resonance with the heave motion. The relative viscous dissipation is quantified with the reflection coefficient of viscous numerical results, while the radiation damping is quantified based on a specially designed radiation potential model with inputs of viscous numerical solutions

Enhanced nutrient removal from mixed black water by a microbial ultra-low weak electrical stimulated anaerobic-two stage anoxic/aerobic process

International audienceEfficient nutrient removal from mixed black water is under challenge in the context of global pollution control and circular economy. The anaerobic-anorexic-aerobic process is a widely used biological nutrient removal technology for wastewater treatment, yet its denitrification capacity is limited by the low biodegradable organics to nitrogen (C/N) ratio due to prior degradation of mixed black water. We have previously proved that weak electrical of 0.2 V could stimulate microbial denitrification of black water using an external electrical supply to ensure voltage stability. To reduce the dependence on additional electrical and to ensure a stable voltage output, we built a microbial fuel cell (MFC) and a microbial electrolytic cell (MEC) embedded in the anaerobic-two stage anoxic/aerobic (A-(A/O) 2) system in this study, where the MFC degrades organic matter and generates electricity first. Then, the electroactive bacteria in the MEC catalyze denitrification at low carbon levels, which is affected by microbial ultra-low weak electrical stimulation (MUlWES) generated by MFC. Results showed that the removal rates of total nitrogen (TN) and total phosphorus (TP) were up to 91.3% and 98.3% respectively at a stimulation voltage of 0.1 V. Fluorescence spectroscopy revealed the formation of aromatic proteins and an increase in tightly bound-extracellular polymeric substances (TB-EPS), suggesting the involvement of these compounds in electron transfer. Community analysis disclosed the activation of autotrophic denitrifying bacteria and the inhibition of most heterotrophic denitrifying bacteria

Synthesis of Macroporous Magnetic Fe3O4 Microparticles Via a Novel Organic Matter Assisted Open-Cell Hollow Sphere Assembly Method

Macroporous magnetic Fe3O4 microparticles, which might act as both drug carriers and magnetocaloric media, were expected to have broad application prospects on magnetocaloric-responsively controlled drug release systems. A kind of macroporous magnetic Fe3O4 microparticle was prepared by an organic matter assisted open-cell hollow sphere (hollow sphere with holes on shell) assembly method in this study. 1-vinyl-2-pyrrolidinone (NVP) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were selected as the template and the binder, respectively. Ferrous ions were specifically bound to carbonyl groups on NVP and were then reduced by NaBH4. The reduced irons underwent heterogeneous nucleation and grain growth to form Fe0/Fe3O4 microspheres consisting of a lot of nano-Fe0 grains, and were then assembled into Fe0/Fe3O4 microparticles wrapped by AMPS. Results indicate that NVP binding with ferrous ions can promote a self-polymerization process and the formation of Fe0/Fe3O4 microspheres, while AMPS enwrapping around the resultant microspheres can facilitate their assembly into larger aggregates. As a result, macroporous Fe3O4 microparticles composed of several open-cell hollow Fe3O4 microspheres can be obtained under a Kirkendall-controlled oxidation. Moreover, these as-prepared macroporous Fe3O4 microparticles possess a narrow particle size distribution and exhibit ferromagnetism (Ms = 66.14 emu/g, Mr = 6.33 emu/g, and Hc = 105.32 Oe). Our work, described here, would open up a novel synthesis method to assemble macroporous magnetic Fe3O4 microparticles for potential application in magnetocaloric-responsively controlled drug release systems

Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses?

Periductal mastitis (PDM) is a prolonged inflammatory disease, but the cause of PDM is poorly understood. In the present case control study, 87 PDM and 87 healthy controls were enrolled and the results were evaluated to identify the significant risk factors for PDM. To investigate the roles of bacterial infection and critical cytokines expression, 16S rRNA gene sequencing and bacterial culturing were conducted. We also measured the levels of interferon-γ, interleukin-12A, and interleukin-17A by semiquantitative immunohistochemistry method. In a multivariable logistic regression model, we identified overweight/obesity and late onset of menarche as independent risk factors for PDM. In contrast, age of first birth >27 years had a protective effect. With 16S rRNA gene sequencing, we confirmed bacterial infections were found in all PDM patients, but none of the control patients was positive on the gene expression of 16S rRNA. Our results also demonstrated significant increases of the IFN-γ and IL-12A expression in PDM, but there was no difference in IL-17A expression in these two groups. Taken together, this study suggests that reproductive factors and overweight/obesity are possible predisposing risk factors for PDM. Bacterial infection and the increased expression of some proinflammatory cytokines are associated with the pathogenesis of this disease