Lipidomic analysis of patients with microbial invasion of the amniotic cavity reveals upâ regulation of leukotriene B4

Bioactive lipids derived from the metabolism of polyunsaturated fatty acids are important mediators of the inflammatory response. Labor per se is considered a sterile inflammatory process. Intraâ amniotic inflammation (IAI) due to microorganisms (i.e., intraâ amniotic infection) or danger signals (i.e., sterile IAI) has been implicated in the pathogenesis of preterm labor and clinical chorioamnionitis at term. Early and accurate diagnosis of microbial invasion of the amniotic cavity (MIAC) requires analysis of amniotic fluid (AF). It is possible that IAI caused by microorganisms is associated with a stereotypic lipidomic profile, and that analysis of AF may help in the identification of patients with this condition. To test this hypothesis, we analyzed the fatty acyl lipidome of AF by liquid chromatographyâ mass spectrometry from patients in spontaneous labor at term and preterm gestations. We report that the AF concentrations of proinflammatory lipid mediators of the 5â lipoxygenase pathway are significantly higher in MIAC than in cases of sterile IAI. These results suggest that the concentrations of 5â lipoxygenase metabolites of arachidonic acid, 5â hydroxyeicosatetraenoic acid, and leukotriene B4 in particular could serve as potential biomarkers of MIAC. This finding could have important implications for the rapid identification of patients who may benefit from antimicrobial treatment.â Maddipati, K. R., Romero, R., Chaiworapongsa, T., Chaemsaithong, P., Zhou, S.â L., Xu, Z., Tarca, A. L., Kusanovic, J. P., Gomez, R., Chaiyasit, N., Honn, K. V. Lipidomic analysis of patients with microbial invasion of the amniotic cavity reveals upâ regulation of leukotriene B4. FASEBJ. 30, 3296â 3307 (2016). www.fasebj.orgPeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154488/1/fsb2fasebj30100583.pd

Chromatin topology reorganization and transcription repression by PML-RARα in acute promyeloid leukemia.

BACKGROUND: Acute promyeloid leukemia (APL) is characterized by the oncogenic fusion protein PML-RARα, a major etiological agent in APL. However, the molecular mechanisms underlying the role of PML-RARα in leukemogenesis remain largely unknown. RESULTS: Using an inducible system, we comprehensively analyze the 3D genome organization in myeloid cells and its reorganization after PML-RARα induction and perform additional analyses in patient-derived APL cells with native PML-RARα. We discover that PML-RARα mediates extensive chromatin interactions genome-wide. Globally, it redefines the chromatin topology of the myeloid genome toward a more condensed configuration in APL cells; locally, it intrudes RNAPII-associated interaction domains, interrupts myeloid-specific transcription factors binding at enhancers and super-enhancers, and leads to transcriptional repression of genes critical for myeloid differentiation and maturation. CONCLUSIONS: Our results not only provide novel topological insights for the roles of PML-RARα in transforming myeloid cells into leukemia cells, but further uncover a topological framework of a molecular mechanism for oncogenic fusion proteins in cancers

Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

Review aricleGanoderma genus comprises one of the most commonly studied species worldwide, G. lucidum. However, other Ganoderma species have been also reported as important sources of bioactive compounds. Polysaccharides are important contributors to the medicinal properties reported for Ganoderma species, as demonstrated by the numerous publications, including reviews, on this matter. Yet, what are the chemical features of Ganoderma polysaccharides that have bioactivity? In the present manuscript, the chemical features of Ganoderma polysaccharides with reported antioxidant, antitumor and antimicrobial activities (the most studied worldwide) are analyzed in detail. The composition of sugars (homo- versus hetero-glucans and other polysaccharides), type of glycosidic linkages, branching patterns, and linkage to proteins are discussed. Methods for extraction, isolation and identification are evaluated and, finally, the bioactivity of polysaccharidic extracts and purified compounds are discussed. The integration of data allows deduction of structure-activity relationships and gives clues to the chemical aspects involved in Ganoderma bioactivity

Responses of crop yield and water use efficiency to climate change in the North China Plain

Based on future climate change projections offered by IPCC, the responses of yields and water use efficiencies of wheat and maize to climate change scenarios are explored over the North China Plain. The climate change projections of 21st century under A2A, B2A and A1B are from HadCM3 global climate model. A climate generator (CLIGEN) is applied to generate daily weather data of selected stations and then the data is used to drive CERES-Wheat and Maize models. The impacts of increased temperature and CO2 on wheat and maize yields are inconsistent. Under the same scenario, wheat yield ascended due to climatic warming, but the maize yield descended. As a more probable scenario, climate change under B2A is moderate relative to A2A and A1B. Under B2A in 2090s, average wheat yield and maize yield will respectively increase 9.8% and 3.2% without CO2 fertilization in this region. High temperature not only affects crop yields, but also has positive effect on water use efficiencies, mainly ascribing to the evapotranspiration intensification. There is a positive effect of CO2 enrichment on yield and water use efficiency. If atmospheric CO2 concentration reaches nearly 600ppm, wheat and maize yields will increase 38% and 12% and water use efficiencies will improve 40% and 25% respectively, in comparison to those without CO2 fertilization. However, the uncertainty of crop yield is considerable under future climate change scenarios and whether the CO2 fertilization may be realized is still needed further research.Climate generator model (CLIGEN) Crop yield Climate change scenario CERES model

Dynamic Simulation and Parameter Analysis of Contact Mechanics for Mimicking Geckos’ Foot Setae Array

According to the dynamic characteristics of the adhesion desorption process between gecko-like polyurethane setae and the contact surface, the microcontact principle of an elastic sphere and plane is established based on the Johnson–Kendall–Robert model. On this basis, combined with the cantilever beam model, microscale adhesive contact models in the case of a single and an array of setae are obtained. The contact process is numerically simulated and verified by the adhesion desorption test. After that, the effects of external preload, the elastic modulus of setae material, the surface energy, and the surface roughness on the contact force and depth during the dynamic contact process of setae are studied. The results show that the error between the simulation and test is 15.9%, and the simulation model could reflect the real contact procedure. With the increase in preload, the push-off force of the setae array would grow and remain basically constant after reaching saturation. Increasing the elastic modulus of setae material would reduce the contact depth, but have little effect on the maximum push-off force; with the increase in the surface energy of the contact object, both the push-off force between the objects and the contact depth during desorption would increase. With the increase in wall roughness, the push-off force curve of the setae array becomes smoother, but the maximum push-off force would decrease. By exploring the dynamic mechanical characteristics of the micro angle of setae, the corresponding theoretical basis is provided for the numerical simulation of the adsorption force of macro materials

Dynamic Simulation and Parameter Analysis of Contact Mechanics for Mimicking Geckos’ Foot Setae Array

According to the dynamic characteristics of the adhesion desorption process between gecko-like polyurethane setae and the contact surface, the microcontact principle of an elastic sphere and plane is established based on the Johnson–Kendall–Robert model. On this basis, combined with the cantilever beam model, microscale adhesive contact models in the case of a single and an array of setae are obtained. The contact process is numerically simulated and verified by the adhesion desorption test. After that, the effects of external preload, the elastic modulus of setae material, the surface energy, and the surface roughness on the contact force and depth during the dynamic contact process of setae are studied. The results show that the error between the simulation and test is 15.9%, and the simulation model could reflect the real contact procedure. With the increase in preload, the push-off force of the setae array would grow and remain basically constant after reaching saturation. Increasing the elastic modulus of setae material would reduce the contact depth, but have little effect on the maximum push-off force; with the increase in the surface energy of the contact object, both the push-off force between the objects and the contact depth during desorption would increase. With the increase in wall roughness, the push-off force curve of the setae array becomes smoother, but the maximum push-off force would decrease. By exploring the dynamic mechanical characteristics of the micro angle of setae, the corresponding theoretical basis is provided for the numerical simulation of the adsorption force of macro materials

A Fault Estimation Method Based on UIO Robust Residual Generators

This paper proposed a fault estimation method based on robust residual generators for a linear system. A system with additive actuator or component faults was considered in the case where the number of the independent faults was larger than that of the independent measurements. This method was achieved based on UIO robust residual generators and there was no need to design extra fault estimators. In this method, fault estimation was achieved via three steps. First, codingsets, which describe the sensitivity relationship between faults and generators, are designed. Second, a bank of robust residual generators are designed according to the coding sets. Finally, fault estimation is achieved by using the result of fault isolation and the output of robust residual generators. A sufficient condition on the application of the method was given and the asymptotic convergence property of the estimation error by using the method was proved. Simulation results demonstrate the effectiveness of the method

A Fault Estimation Method Based on UIO Robust Residual Generators

This paper proposed a fault estimation method based on robust residual generators for a linear system. A system with additive actuator or component faults was considered in the case where the number of the independent faults was larger than that of the independent measurements. This method was achieved based on UIO robust residual generators and there was no need to design extra fault estimators. In this method, fault estimation was achieved via three steps. First, codingsets, which describe the sensitivity relationship between faults and generators, are designed. Second, a bank of robust residual generators are designed according to the coding sets. Finally, fault estimation is achieved by using the result of fault isolation and the output of robust residual generators. A sufficient condition on the application of the method was given and the asymptotic convergence property of the estimation error by using the method was proved. Simulation results demonstrate the effectiveness of the method