Passengers' likely behaviour based on demographic difference during an emergency evacuation in a Ro-Ro passenger ship

By examining the characteristics of passengers on a ship route between the Shandong and Liaodong Peninsula, through a questionnaire survey, this paper aims to address the likely behaviours of passengers during emergency evacuation and the demographic differences among these behaviours. A questionnaire survey of 1380 passengers shows that passengers on board are more alert and are more likely to proactively respond to evacuation alarms (62.5%), observe others’ actions (59.1%), follow evacuation instructions (67.9%), obey the crew (66.2%), queue patiently (63%), return to the cabin when their families are left behind (65.1%), and be cooperative (59%) rather than competitive (44%). The multinomial logistic regression results show that passengers who are older, with limited mobility, that have more experience aboard ships and are part of a larger group, will be more likely to proactively confirm the authenticity of evacuation events. Men, elderly individuals, people who are part of a larger group and with less experience in evacuation education are more likely to follow others. When the family is left behind, elderly individuals and people who are part of a larger group are much more likely to choose to return to their cabins. Similarly, elderly passengers with larger groups are much more likely to choose to help others. Although questionnaire research has some limitations, such as a hypothetical response and closed questions, the research results are of great significance for helping passenger ship managers to develop appropriate management rules, and conduct effective evacuation education activities

Reproductive toxicity and underlying mechanisms of fine particulate matter (PM2.5) on Caenorhabditis elegans in different seasons

Although numerous studies have investigated that atmospheric fine particulate matter (PM2.5) can be toxic to environmental organisms, the research on the reproductive toxicity of PM2.5 is limited, and the key toxic components and underlying mechanisms remain unknown. In this work, PM2.5 samples of four seasons in Nanjing from March 1, 2021, to February 28, 2022 were collected and the chemical components were analyzed. Caenorhabditis elegans (C. elegans) was employed to conduct the toxicological testing. The reproductive toxicity of PM2.5 to C. elegans in different seasons was evaluated by multiple reproductive endpoints. Exposure to high concentrations of PM2.5 significantly decreased the brood size and the number of fertilized eggs in utero. PM2.5 exposure also increased the number of germ cell corpses and caused abnormal expression of apoptosis-related genes (ced-9, ced-4, and ced-3), which confirmed that PM2.5 induced germline apoptosis. In addition, PM2.5 exposure significantly increased the production of reactive oxygen species (ROS) in C. elegans and the fluorescence intensity of HUS-1 protein in of transgenic strain WS1433. Meanwhile, the expression of genes related to DNA damage (cep-1, clk-2, egl-1, and hus-1) and oxidative stress (mev-1, isp-1, and gas-1) also significantly altered in C. elegans, suggesting induction of DNA damage and oxidative stress. According to Pearson correlation analyses, DNA damage and oxidative stress were significantly correlated with multiple reproductive endpoints in C. elegans. Thus, it was speculated that PM2.5 caused reproductive dysfunction and germ cell apoptosis in C. elegans may be by inducing ROS and DNA damage. In addition, heavy metals in PM2.5 were significantly correlated with multiple endpoints at physiological and biochemical, suggesting that the heavy metals might be an important contributor to the reproductive toxicity induced by PM2.5

Petrogenesis of the Early Cretaceous Tietonggou Diorite and Its Geological Implications

The Tietonggou pluton is mainly composed of gabbroic diorite and diorite. The petrology, zircon U-Pb age, and geochemistry of the Tietonggou diorite have been studied to determine its petrogenesis and metallogenic significance. The diorite samples have 56–58 wt% SiO2 and 11–14 wt% Al2O3 and are peraluminous and sodic (Na2O/K2O = 1.29–2.07). All the samples are enriched in light rare earth elements (LREEs) and large-ion lithophile elements (LILEs; e.g., Rb, Ba, and Sr) but depleted in heavy rare earth elements (HREEs) and high field strength elements (HFSEs; e.g., Zr, Nb, and Ta), suggesting subduction-related affinities. The rocks have narrow ranges of (206Pb/204Pb)t (18.5–19.0), (207Pb/204Pb)t (15.71–15.75), and (208Pb/204Pb)t (38.4–39.0) ratios, respectively. Zircons from the Tietonggou diorite yielded a weighted average U-Pb age of 132.86 ± 0.92 Ma (MSWD = 0.48), whilst those from the nearby Laowa diorite yielded 129.72 ± 0.61 Ma (MSWD = 1.05). This suggests that the rocks represent Early Cretaceous plutons, coeval with the peak lithospheric thinning in eastern North China Craton (NCC). The magma likely originated from partial melting of the enriched lithospheric mantle and was contaminated by ancient lower NCC crustal materials. Our study clarifies the tectonic background of the Tietonggou pluton and provides support for the study of the genesis of Fe–skarn deposits in western Shandong

Genesis of the Panzijian gold deposit in Jiaodong Peninsula, Eastern China: Insights from fluid inclusion and isotopes

The Panzijian gold deposit is located in the Jiaobei uplift on the eastern margin of North China Craton. It is a quartz vein-type gold deposit in the southern end of the Qixia-Penglai ore belt. In order to explore the source and properties of ore-forming fluids and materials in the Panzijian gold deposit, explore the ore-forming process and reveal the genesis of the deposit, this work has carried out petrographic observation and temperature measurement of gold-bearing quartz fluid inclusions, laser Raman experiment, gold-bearing quartz H–O isotope study, and gold-bearing pyrite Pb isotope study. The Panjian gold deposit is divided into four metallogenic stages and the metallogenic stage Ⅲ is the main gold mineralization stage. Fluid inclusions show that the ore-forming fluids at the Panzijian gold deposit belong to a CO2–H2O–NaCl system with low temperature (172–341 °C), salinity (1.57–10.49 wt% NaCl), and density (0.79–0.96 g/cm3). Gold-bearing Quartz H–O isotopic data (δD = −79.8 to −65.1‰, δ18OH2O = 1.33–2.63‰) show that the ore-forming fluid was derived from the mixing of magmatic water and meteoric water. Gold-bearing Pyrite Pb isotopes with crustal signature (206Pb/204Pb = 16.06–16.943, 207Pb/204Pb = 15.337–15.858, 208Pb/204Pb = 37.143–38.081, and 206Pb/207Pb = 1.025–1.105) support that some ore-forming materials were crust-derived. We proposed a metallogenic model for the Panzijian gold deposit: Stress transition of Jiaobei uplift tectonic system inducing asthenospheric upwelling. The lithostatic pressure dropped and fluids were exsolved from the magma. The ore-forming fluid ascended along local faults and the pressure further dropped, resulting in fluid immiscibility and gold deposition along structural traps (e.g., faults)

CircEZH2 Regulates Milk Fat Metabolism through miR-378b Sponge Activity

In this study, we evaluated the roles of heat-induced circEZH2 in the regulation of milk fat metabolism. CircEZH2 overexpression increased HC11 cell proliferation and decreased apoptosis. These changes were accompanied by increased expression of proliferation marker proteins (PCNA, Cyclin D, and Cyclin E) and the anti-apoptotic protein Bcl2, while expression of the pro-apoptotic proteins Bax and cleaved-caspase was reduced. SiRNA-mediated silencing of EZH2 in HC11 cells had the opposite effects. CircEZH2 overexpression promoted the uptake of a fluorescent fatty acid (Bodipy) as well as expression of the fatty acid transport-related protein CD36, lipolysis-related protein LPL, and unsaturated fatty acid metabolism-related proteins FADS1 and SCD1. Dual luciferase reporter assays verified the targeting relationship of the two ceRNA networks, circEZH2-miR378b-LPL and circEZH2-miR378b-CD36. This information provides further clarification of the role of circRNAs in milk fat regulation in addition to a theoretical basis for alleviating the effects of heat stress on milk production by dairy cows

U–Pb dating and Hf isotope study of detrital zircons from the Zhifu Group, Jiaobei Terrane, North China Craton: Provenance and implications for Precambrian crustal growth and recycling

The Zhifu Group exposed in the northeastern Jiaobei Terrane is subdivided from base to top into the Laoyeshan, Bingying and Dongkou formations, and comprises mainly quartzites and muscovite- and tourmaline-bearing quartz schists. In order to define its provenance and depositional age, and to constrain the crustal growth and recycling of the source regions, we have conducted LA-ICP-MS U-Pb dating and in situ Hf isotopic study of detrital zircons from three quartz schists samples collected from the Zhifu Group. Most of the analyzed zircon grains have oscillatory zoning and Th/U ratios >0.4, suggesting igneous origins. 270 detrital zircons U-Pb analyses yield U-Pb ages of 1709-3679 Ma that cluster into three major age populations of 1.7-1.9 Ga with a peak at 1844 Ma, 1.9-2.0 Ga with a peak at 1936 Ma and 2.4-2.55 Ga with a peak at 2465 Ma, and two minor age populations of 2.2-2.35 and 2.6-2.7 Ga. The peaking age of 1844 Ma for the youngest age population indicates that the Zhifu Group must be deposited after 1844 Ma. Detrital zircons with ages of 1.7-2.35 Ga can be linked to pre- and post-tectonic polyphase magmatic events along the JLJB, and most of the zircons have negative epsilon(Hf(t)) values from +1.76 to -18.02 and two-stage Hf model ages (T-DM2) concentrating at similar to 2.7-3.0 Ga, indicating that source rocks of these zircons were derived from recycling of ancient crusts. Detrital zircons with ages of 2.4-2.9 Ga may have been sourced from the Archean granitoid (mainly TTG) gneisses in the Jiaobei Terrane, and these zircons have epsilon(Hf(t)) values from +6.4 to -7.53 and T-Dm2 of 2.6-3.4 Ga (peak at similar to 2.7-3.0 Ga), suggesting that source rocks of these zircons were mainly derived from juvenile crusts associated with a major crustal growth at similar to 2.7-3.0 Ga, and a limited amount of ancient crustal component. The two detrital zircons with ages of 3631 and 3679 Ma have negative epsilon(Hf(t)) values of -4.42 and -1.51 and T-Dm2 of 4137 and 4000 Ma, respectively, implying the recycling of ancient continental crust (>3.6 Ga) and crustal growth prior to 4.1 Ga in the NCC

Shale gas enrichment model and exploration implications in the mountainous complex structural area along the southwestern margin of the Sichuan Basin: A new shale gas area

Marine shale gas in the southern Sichuan Basin is the most successful area of shale gas exploration and development in China. In order to open up new shale gas fields and search for new shale gas reserves and production replacement blocks, it is necessary to continuously establish and complete the standards on shale gas reservoir evaluation and area selection under different structural settings. The early exploration practice shows that shale in the mountainous complex structural area along the southwestern margin of the Sichuan Basin varies greatly in the gas bearing property, so systematical analysis and study on the shale gas enrichment mechanisms in this area is of great significance to searching for new shale gas exploration areas. Based on drilling data of 5 wells in the mountainous complex structural area along the southwestern margin of the Sichuan Basin, the main factors controlling the shale gas bearing property and the shale gas enrichment model were discussed based on the systematical experiments and analysis of the outcrops, cores and cuttings of Longmaxi Formation shale from the aspects of organic geochemistry, physical property, gas content, hydrocarbon generation history and pore evolution history, so as to lay a solid geological basis for the birth of a new shale gas area. And the following research results were obtained. First, the shale of the first submember of first Member of Longmaxi Formation (S1l11) in the mountainous complex structural area along the southwestern margin of the Sichuan Basin has an average TOC of 3.02%–4.97% and Ro of 2.38%–3.37%, and the average total gas content in local enrichment zones is up to 4.62 m3/t, so it is classified as quality shale. Second, the detailed studies on hydrocarbon generation history indicate that the shale has the characteristics of “low thermal evolution rate and low maturity”. It is characterized by late hydrocarbon generation, low thermal evolution rate, low current maturity and short late diffusion time, which are favorable for shale gas enrichment. Third, the pore evolution history reveals that shale pore evolution can be divided into six stages, i.e., sharp pore reduction, pore reduction, organic pore formation, pore preservation, organic pore dissipation and karstification, and organic pore and TOC are the most direct control factors of shale gas content. Fourth, favorable shelf facies belt is conducive to the formation of large-scale reservoir space and effective pores, and diversities of preservation conditions under different structural styles and at different structural positions control different pore evolution stages. The shale gas in the mountainous complex structural area is characterized by horizontal zoning and differential enrichment. “Low thermal evolution rate and low maturity” and “slow structural uplifting” are beneficial to the long-term enrichment and preservation of shale gas. In conclusion, the mountainous complex structural area along the southwestern margin of the Sichuan Basin has a shale gas enrichment model of “sedimentation controlling source rocks, diagenesis controlling reservoirs and structure controlling preservation”. This research result provides idea and reference for searching for new shale gas areas and fields

The machine learning‐based model for lateral lymph node metastasis of thyroid medullary carcinoma improved the prediction ability of occult metastasis

Abstract Background For medullary thyroid carcinoma (MTC) with no positive findings in the lateral neck before surgery, whether prophylactic lateral neck dissection (LND) is needed remains controversial. A better way to predict occult metastasis in the lateral neck is needed. Methods From January 2010 to January 2022, patients who were diagnosed with MTC and underwent primary surgery at our hospital were retrospectively reviewed. We collected the patients' baseline characteristics, surgical procedure, and rescored the ultrasound images of the primary lesions using American College of Radiology (ACR) Thyroid Imaging, Reporting and Data System (TI‐RADS). Regularized logistic regression, 5‐fold cross‐validation and decision curve analysis was applied for lateral lymph node metastasis (LLNM) model's development and validation. Then, we tested the predictive ability of the LLNM model for occult LLNM in cN0−1a patients. Results A total of 218 patients were enrolled. Five baseline characteristics and two TI‐RADS features were identified as high‐risk factors for LLNM: gender, baseline calcitonin (Ctn), tumor size, multifocality, and central lymph node (CLN) status, as well as TI‐RADS margin and level. A LLNM model was developed and showed a good discrimination with 5‐fold cross‐validation mean area under curve (AUC) = 0.92 ± 0.03 in the test dataset. Among cN0−1a patients, our LLNM model achieved an AUC of 0.91 (95% CI, 0.88–0.94) for predicting occult LLNM, which was significantly higher than the AUCs of baseline Ctn (0.83) and CLN status (0.64). Conclusions We developed a LLNM prediction model for MTC using machine learning based on clinical baseline characteristics and TI‐RADS. Our model can predict occult LLNM for cN0−1a patients more accurately, then benefit the decision of prophylactic LND

Comprehensive analysis of single-cell and bulk RNA-sequencing data identifies B cell marker genes signature that predicts prognosis and analysis of immune checkpoints expression in head and neck squamous cell carcinoma

Recent studies have shown that B cells and the associated tertiary lymphoid structures (TLS) correlate with the response of patients to immune checkpoint inhibitors (ICIs) and predict overall survival (OS) in cancer patients. We screened 145 B cell marker genes (BCMG) by a comprehensive analysis of single-cell RNA-sequencing (scRNA-seq) data of head and neck squamous cell carcinoma (HNSC) from the Gene Expression Omnibus (GEO) database. The BCMG signature (BCMGS) was established using The Cancer Genome Atlas (TCGA) dataset of HNSC and verified in four independent datasets. The multivariate Cox regression analysis identified the signature as an independent prognostic factor. A prognostic nomogram was constructed with independent prognostic factors using the TCGA dataset. GO and KEGG analysis revealed the underlying signaling pathways related to this signature. Study of immune profiles showed that patients in the low-risk group presented discriminative immune-cell infiltrations. Furthermore, the low-risk group was featured by higher TCR and BCR diversity, which suggested that low-risk patients may be more sensitive to ICIs. Immunohistochemistry was performed, and we found that high expression of FTH1 was significantly correlated with poor OS (P = 0.025). The expression of TIM-3, LAG-3 and PD-1 was positively correlated and associated with better OS in HNSC. However, there was no statistically significant difference between PD-L1, PD-L2, CTLA-4, TIGIT and prognosis. The BCMGS was a promising prognostic biomarker in HNSC, which may help to interpret the responses to immunotherapy and provide a new perspective for future research on the treatment in HNSC