Numerical Simulation of Chip Formation in Metal Cutting Process

In order to study the chip formation mechanism in metal cutting process, based on finite element software ABAQUS, the paper established finite element model and carried out numerical simulation on serrated chip formation of Ni-base superalloy GH4169 and ribbon chip formation of 45# steel respectively. In addition, this paper also analyzed the influence law of three factors (cutting speed, feed rate, back cutting depth) on cutting force and the distribution rule of cutting heat in serrated chip formation of GH4169

Dietary supplementation of Chinese herbal medicines enhances the immune response and resistance of rainbow trout (Oncorhynchus mykiss) to infectious hematopoietic necrosis virus

Rainbow trout is a widely farmed economical cold-water fish worldwide, but the prevalence of infectious hematopoietic necrosis virus (IHNV) presents a severe risk to the aquaculture industry, resulting in high mortality and huge economic losses. In this study, the impacts of different concentrations (0, 10, 20, and 30 g/kg) of Chinese herbal medicine mixture (CHMM) on the immune response and resistance of rainbow trout to IHNV infection were evaluated. The results show that CHMM noticeably increased (P < 0.05) T-SOD, CAT, AST, ALT, ACP, and AKP activities and decreased MDA content. NF-κB, TNF-α, IFN-β, IL-1β, JAK1, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while SOCS2 expression was downregulated (P < 0.05). Following infection with IHNV, feeding rainbow trout with varying amounts of CHMM resulted in noticeably increased (P < 0.05) T-SOD, ACP, and AKP activities and significantly decreased (P < 0.05) MDA content and AST and ALT activities. TNF-α, IFN-β, IL-1β, HSP70, and HSP90 expressions were significantly upregulated (P < 0.05) in all CHMMs, while the expressions of JAK1 and SOCS2 were downregulated. The expression level of the IHNV G protein gene at a dosage of 20 g/kg was notably lower than that of the other CHMM feeding groups. This study provides a solid scientific basis for promoting CHMM as an immunostimulant for boosting antiviral immunity in rainbow trout

The 2021 FASEB science research conference on NAD metabolism and signaling

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A Probe into Spoken English Recognition in English Education Based on Computer-Aided Comprehensive Analysis

At present, computer-aided spoken English learning is becoming increasingly popular among learners. The computer-aided comprehensive analysis tech-nology can evaluate and correct learner's spoken pronunciation, thereby im-proving their pronunciation. Based on computer-aided comprehensive analy-sis, this paper aims to explore the automatic recognition and scoring methods of spoken English in English education. For this, it studies the effective matching of the feedback information with the known pronunciation scoring results, and then develops a computer evaluation plug-in consisting of dif-ferent modules such as user login, English spoken speech acquisition and recognition, voice evaluation, speech broadcast, and spoken dialogue. The research results show that the computer evaluation plug-in matches and compares the extracted feature parameters of input speech with the standard features, scores the spoken language input by the learner, and gives the cor-rect pronunciation so that the learner can get feedback in time. For different stages of English learning, the focus of recognition technology and the spo-ken recognition algorithms applied also vary. The research findings provide theoretical and technical support for oral English recognition, error correction and scoring

Effects of Supplemental Nucleotides, Taurine, and Squid Liver Paste on Feed Intake, Growth Performance, Serum Biochemical Parameters, and Digestive Enzyme Activities of Juvenile GIFT Tilapia (Oreochromis sp.) Fed Low Fishmeal Diets

This 8 week feeding trial evaluated the effects of three feeding stimulants (FS) on feed intake, growth performance, body composition, serum biochemical parameters, and digestive enzyme activities for juvenile GIFT tilapia fed low fishmeal diets. Four test diets were supplemented with 0, 16 g/kg taurine (Tau), 0.4 g/kg mixed nucleotides (Mix-NT), and 30 g/kg squid liver paste (SQLP) respectively, and fed to juvenile GIFT strain of Oreochromis niloticus (3.34±0.01 g). The results showed that feed intake (FI) significantly increased with SQLP. Specific growth rate (SGR) in fish fed SQLP or Mix-NT diet was significantly higher than that of the Tau and control group. The feed conversion ratio (FCR) ranged from 1.34 in the group fed Mix-NT to 1.50 in the group fed Tau, with no significant differences compared to the control group. Three feeding stimulants had no significant influence on whole body composition of tilapia. Fish fed Mix-NT diet showed significantly higher intestinal protease activity and intestinal amylase activity than that of other groups. High-density lipoprotein content in serum was significantly higher in fish fed SQLP diet than that of other groups. In conclusion, supplementation of 0.4 g/kg mixed nucleotides or 30 g/kg squid liver paste in low fishmeal diets could provide better growth performance for juvenile tilapia, and SQLP could act as an effective FS under these conditions. Supplementation of Mix-NT could reduce the FCR in these fish

Preparation of NiS/ZnIn2S4 as a superior photocatalyst for hydrogen evolution under visible light irradiation

In this study, NiS/ZnIn2S4 nanocomposites were successfully prepared via a facile two-step hydrothermal process. The as-prepared samples were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HRTEM). Their photocatalytic performance for hydrogen evolution under visible light irradiation was also investigated. It was found that the photocatalytic hydrogen evolution activity over hexagonal ZnIn2S4 can be significantly increased by loading NiS as a co-catalyst. The formation of a good junction between ZnIn2S4 and NiS via the two step hydrothermal processes is beneficial for the directional migration of the photo-excited electrons from ZnIn2S4 to NiS. The highest photocatalytic hydrogen evolution rate (104.7 μmol/h), which is even higher than that over Pt/ZnIn2S4 nanocomposite (77.8 μmol/h), was observed over an optimum NiS loading amount of 0.5 wt %. This work demonstrates a high potential of the developing of environmental friendly, cheap noble-metal-free co-catalyst for semiconductor-based photocatalytic hydrogen evolution

Comparative transcriptomics combined with physiological and functional analysis reveals the regulatory mechanism of rainbow trout (Oncorhynchus mykiss) under acute hypoxia stress

Hypoxia, largely triggered by global warming and water contamination, has become an environmental issue of great concern, posing a great threat to aquatic ecosystem. As one of the world's most economically important fish, rainbow trout (Oncorhynchus mykiss) is extremely intolerant of hypoxic environments, however, little is known about the roles of non-coding RNAs (ncRNAs) in the response of rainbow trout to hypoxia stress. Herein, effects of moderate (Tm12L) and severe hypoxia for 12 h (Ts12L) and 12 h reoxygenation on histology, biochemical parameters (antioxidant, metabolism and immunity) and transcriptome (lncRNA, miRNA and mRNA) in rainbow trout liver were investigated. We further validated the regulatory relationships between LOC110519952, novel-m0023-5p and glut1a via dual‑luciferase reporter, overexpression and silencing assays. Compared with Tm12L, the liver in Ts12L showed more severe oxidative damage. Anaerobic, lipid and protein metabolism was enhanced under hypoxia stress, especially in Ts12L. We also found that Tm12L could strengthen innate immune response, which was inhibited in Ts12L. Besides, several hypoxia-related genes (glut1a, vegfaa, hmox, epoa, foxo1a and igfbp1) and ceRNA networks were identified from 1824, 427 and 545 differentially expressed mRNAs, miRNAs and lncRNAs, including LOC118965299-novel-m0179-3p-epoa, LOC110519952-novel-m0023-5p-glut1a, MSTRG.7382.2-miR-184-y-hmox and LOC110520012-miR-206-y-vegfaa. Through in vitro and in vivo functional analysis, we demonstrated that glut1a is a target of novel-m0023-5p, and LOC110519952 can positively regulate glut1a by targeting novel-m0023-5p. Introduction of LOC110519952 could attenuate the promoting effects of novel-m0023-5p on rainbow trout liver cell viability and proliferation. This study highlights the differences in the regulatory mechanism of rainbow trout under different concentrations of hypoxia stress and provides valuable data for further research on the molecular mechanisms of fish adaptation to hypoxic environments

Numerical Simulation of Chip Formation in Metal Cutting Process

In order to study the chip formation mechanism in metal cutting process, based on finite element software ABAQUS, establish finite element model, and carry out numerical simulation on serrated chip formation of Ni-base superalloy GH4169 and ribbon chip formation of 45# steel respectively.In addition, analyze the influence law of three factors (cutting speed, feed rate, back cutting depth) on cutting force and the distribution rule of cutting heat in serrated chip formation of GH4169.DOI: http://dx.doi.org/10.11591/telkomnika.v10i3.608

Evolution of the Probe-Based Loop-Mediated Isothermal Amplification (LAMP) Assays in Pathogen Detection

Loop-mediated isothermal amplification (LAMP), as the rank one alternative to a polymerase chain reaction (PCR), has been widely applied in point-of-care testing (POCT) due to its rapid, simple, and cost-effective characteristics. However, it is difficult to achieve real-time monitoring and multiplex detection with the traditional LAMP method. In addition, these approaches that use turbidimetry, sequence-independent intercalating dyes, or pH-sensitive indicators to indirectly reflect amplification can result in false-positive results if non-specific amplification occurs. To fulfill the needs of specific target detection and one-pot multiplex detection, a variety of probe-based LAMP assays have been developed. This review focuses on the principles of these assays, summarizes their applications in pathogen detection, and discusses their features and advantages over the traditional LAMP methods

Optimization of Processing Parameters and Adhesive Properties of Aluminum Oxide Thin-Film Transition Layer for Aluminum Substrate Thin-Film Sensor

A thin-film strain micro-sensor is a cutting force sensor that can be integrated with tools. Its elastic substrate is an important intermediate to transfer the strain generated by the tools during cutting to the resistance-grid-sensitive layer. In this paper, 1060 aluminum is selected as the elastic substrate material and aluminum oxide thin film is selected as the transition layer between the aluminum substrate and the silicon nitride insulating layer. The Stoney correction formula applicable to the residual stress of the aluminum oxide film is derived, and the residual stress of the aluminum oxide film on the aluminum substrate is obtained. The influence of Sputtering pressure, argon flow and negative substrate bias process parameters on the surface quality and sputtering power of the aluminum oxide thin film is discussed. The relationship model between process parameters, surface roughness, and sputtering rate of thin films is established. The sputtering process parameters for preparing an aluminum oxide thin film are optimized. The micro-surface quality of the aluminum oxide thin film obtained before and after the optimization of the process parameters and the surface quality of Si3N4 thin film sputtered on alumina thin film before and after the optimization are compared. It is verified that the optimized process parameters of aluminum oxide film as a transition layer can improve the adhesion between the insulating-layer silicon nitride film and the aluminum substrate