Desarrollo e implementación de un hormigón reforzado con fibras sintéticas para la repavimentación de la Ruta 24 de Uruguay

Los pavimentos de hormigón son estructuras propensas a la fisuración. El uso de fibras aparece como una de las mejores alternativas técnico-económicas para mejorar el comportamiento del hormigón. Una de las aplicaciones del Hormigón Reforzado con Fibras (HRF) es la reparación o refuerzo (“overlays”) de antiguos pavimentos. En la actualidad se dispone de macrofi bras sintéticas que pueden utilizarse en este tipo de aplicaciones en lugar de las tradicionales fi bras de acero. Este trabajo transmite la experiencia adquirida durante la ejecución de la rehabilitación de la Ruta 24 en Uruguay empleando un “whitetopping” delgado (adherido) de HRF. Se describen los resultados obtenidos durante el control de calidad en obra y se discuten los principales desafíos durante la ejecución como el uso de un aditivo reductor de agua de alto rango, el empleo de tecnología de alto rendimiento y el logro de una adecuada adherencia HRF – sustrato asfáltico.Fil: Miguez Pessada, D.. Hormigones Artigas S.A.; UruguayFil: Gonzalez, A. C. Hormigones Artigas S.A.; UruguayFil: Violini, D.. Cementos Avellaneda S.A; ArgentinaFil: Pappalardi, M.. Cementos Avellaneda S.A.; ArgentinaFil: Zerbino, Raul Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ingeniería. Departamento de Construcciones; Argentin

Genome Sequence of Stenotrophomonas maltophilia PML168, Which Displays Baeyer-Villiger Monooxygenase Activity

Stenotrophomonas maltophilia PML168 was isolated from Wembury Beach on the English Coast from a rock pool following growth and selection on agar plates. Here we present the permanent draft genome sequence, which has allowed prediction of function for several genes encoding enzymes relevant to industrial biotechnology, including a novel flavoprotein monooxygenase

Sequence Ontology terminology for gene regulation

The Sequence Ontology (SO) is a structured, controlled vocabulary that provides terms and definitions for genomic annotation. The Gene Regulation Ensemble Effort for the Knowledge Commons (GREEKC) initiative has gathered input from many groups of researchers, including the SO, the Gene Ontology (GO), and gene regulation experts, with the goal of curating information about how gene expression is regulated at the molecular level. Here we discuss recent updates to the SO reflecting current knowledge. We have developed more accurate human-readable terms (also known as classes), including new definitions, and relationships related to the expression of genes. New findings continue to give us insight into the biology of gene regulation, including the order of events, and participants in those events. These updates to the SO support logical reasoning with the current understanding of gene expression regulation at the molecular level

Gold oxide films grown in the confined aqueous layer between gold and organic solvents

The properties of anodic passive films potentiostatically formed on polycrystalline gold in aqueous phosphate solutions were studied using voltammetry, electrochemical impedance spectroscopy, and contact angle measurements. The nature of the gold oxide layer was analyzed as a function of a potential holding in the aqueous double layer charge region at the interface between gold and the aqueous layer confined by insoluble organic solvents (hexane, chloroform, anisole, butyl acetate, xylene, and isopropyl ether). Different growth conditions change the homogeneity of the oxide layer leading to different passive properties. A synergetic effect on the gold oxidation of hydrogen dissolved in both the bulk metal and the confined aqueous layer is discussed.Instituto de Investigaciones Fisicoquímicas Teóricas y AplicadasInstituto de Física de Líquidos y Sistemas Biológico

Deflections in beams of Argentinean Eucalyptus grandis under long-term loading

The present paper reports the results of an investigation regarding the analysis of deflections in structural-sized beams of fast-growth Argentinean Eucalyptus grandis under a one-year loading and in indoor climate. An empirical research project with a sample containing 16 beams was carried out. 8 pieces were free of pith and another 8 pieces contained pith. The results obtained allow to compare the creep behaviour of this timber species with those reported by other researchers for both structural-sized and small-clear specimens. The research confirms the design rules adopted by Eurocode 5 for calculating the creep deflections of this timber species under a long-term load. Results also evidence a slight influence of the presence of pith on creep, confirming similar creep behaviour for different qualities of this timber species

Simultaneous genome sequencing of symbionts and their hosts

Second-generation sequencing has made possible the sequencing of genomes of interest for even small research groups. However, obtaining separate clean cultures and clonal or inbred samples of metazoan hosts and their bacterial symbionts is often difficult. We present a computational pipeline for separating metazoan and bacterial DNA in silico rather than at the bench. The method relies on the generation of deep coverage of all the genomes in a mixed sample using Illumina short-read sequencing technology, and using aggregate properties of the different genomes to identify read sets belonging to each. This inexpensive and rapid approach has been used to sequence several nematode genomes and their bacterial endosymbionts in the last year in our laboratory and can also be used to visualize and identify unexpected contaminants (or possible symbionts) in genomic DNA samples. We hope that this method will enable researchers studying symbiotic systems to move from gene-centric to genome-centric approaches

Ellipsometry of nickel hydroxide electrodes formed by ex situ chemical precipitation : Potential routine and time effects

Ellipsometric and reflectivity data combined with voltammetric data have been obtained at 546.1 nm for different nickel hydroxide electrodes in 0.01 M NaOH at 25°C. The electrodes were prepared by ex situ chemical precipitation on a polished platinum substrate. The influence of the potential routine applied to the nickel hydroxide electrode on the corresponding optical parameters and time effects for both the oxidized and reduced forms of the active material kept at constant potential have been investigated. The nickel hydroxide layer is described as a mixture of nickel(II) hydroxide, nickel(III) hydroxide and electrolyte—water domains. The kinetic effects are discussed through a complex reaction pathway involving different nickel(II) and nickel(III) hydroxide species, the processes starting preferentially at the borders of the different domains.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicada

Desarrollo e implementación de un hormigón reforzado con fibras sintéticas para la repavimentación de la Ruta 24 de Uruguay

Los pavimentos de hormigón son estructuras propensas a la fisuración. El uso de fibras aparece como una de las mejores alternativas técnico-económicas para mejorar el comportamiento del hormigón. Una de las aplicaciones del Hormigón Reforzado con Fibras (HRF) es la reparación o refuerzo (“overlays”) de antiguos pavimentos. En la actualidad se dispone de macrofi bras sintéticas que pueden utilizarse en este tipo de aplicaciones en lugar de las tradicionales fi bras de acero. Este trabajo transmite la experiencia adquirida durante la ejecución de la rehabilitación de la Ruta 24 en Uruguay empleando un “whitetopping” delgado (adherido) de HRF. Se describen los resultados obtenidos durante el control de calidad en obra y se discuten los principales desafíos durante la ejecución como el uso de un aditivo reductor de agua de alto rango, el empleo de tecnología de alto rendimiento y el logro de una adecuada adherencia HRF – sustrato asfáltico.Facultad de IngenieríaLaboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológic

Safe and complete contig assembly via omnitigs

Contig assembly is the first stage that most assemblers solve when reconstructing a genome from a set of reads. Its output consists of contigs -- a set of strings that are promised to appear in any genome that could have generated the reads. From the introduction of contigs 20 years ago, assemblers have tried to obtain longer and longer contigs, but the following question was never solved: given a genome graph $G$ (e.g. a de Bruijn, or a string graph), what are all the strings that can be safely reported from $G$ as contigs? In this paper we finally answer this question, and also give a polynomial time algorithm to find them. Our experiments show that these strings, which we call omnitigs, are 66% to 82% longer on average than the popular unitigs, and 29% of dbSNP locations have more neighbors in omnitigs than in unitigs.Comment: Full version of the paper in the proceedings of RECOMB 201