661 research outputs found
Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by dual-wavelength photoacoustic microscopy
Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme ÎČ-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 ÎŒm. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy
Simultaneous imaging of a lacZ-marked tumor and microvasculature morphology in vivo by dual-wavelength photoacoustic microscopy
Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme ÎČ-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 ÎŒm. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy
Integrated monitoring of mountain glaciers as key indicators of global climate change: the European Alps
The internationally recommended multi-level strategy for monitoring mountain glaciers is illustrated using the example of the European Alps, where especially dense information has been available through historical times. This strategy combines in situ measurements (mass balance, length change) with remote sensing (inventories) and numerical modelling. It helps to bridge the gap between
detailed local process-oriented studies and global coverage. Since the 1980s, mass balances have become increasingly negative, with values close to â1 m w.e. aâ1 during the first 5 years of the 21st century. The hot, dry summer of 2003 alone caused a record mean loss of 2.45 m w.e., roughly 50% above the previous record loss in 1998, more than three times the average between 1980 and 2000 and an order of magnitude more than characteristic long-term averages since the end of the Little Ice Age and other extended periods of glacier shrinkage during the past 2000 years. It can be estimated that glaciers in the European Alps lost about half their total volume (roughly 0.5% aâ1) between 1850 and
around 1975, another 25% (or 1% aâ1) of the remaining amount between 1975 and 2000, and an additional 10â15% (or 2â3% aâ1) in the first 5 years of this century
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On the importance of cascading moisture recycling in South America
Continental moisture recycling is a crucial process of the South American climate system. In particular, evapotranspiration from the Amazon basin contributes substantially to precipitation regionally as well as over other remote regions such as the La Plata basin. Here we present an in-depth analysis of South American moisture recycling mechanisms. In particular, we quantify the importance of cascading moisture recycling (CMR), which describes moisture transport between two locations on the continent that involves re-evaporation cycles along the way. Using an Eulerian atmospheric moisture tracking model forced by a combination of several historical climate data sets, we were able to construct a complex network of moisture recycling for South America. Our results show that CMR contributes about 9â10% to the total precipitation over South America and 17â18% over the La Plata basin. CMR increases the fraction of total precipitation over the La Plata basin that originates from the Amazon basin from 18â23 to 24â29% during the wet season. We also show that the south-western part of the Amazon basin is not only a direct source of rainfall over the La Plata basin, but also a key intermediary region that distributes moisture originating from the entire Amazon basin towards the La Plata basin during the wet season. Our results suggest that land use change in this region might have a stronger impact on downwind rainfall than previously thought. Using complex network analysis techniques, we find the eastern side of the sub-tropical Andes to be a key region where CMR pathways are channeled. This study offers a better understanding of the interactions between the vegetation and the atmosphere on the water cycle, which is needed in a context of land use and climate change in South America
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Self-amplified Amazon forest loss due to vegetation-atmosphere feedbacks
Reduced rainfall increases the risk of forest dieback, while in return forest loss might intensify regional droughts. The consequences of this vegetation-atmosphere feedback for the stability of the Amazon forest are still unclear. Here we show that the risk of self-amplified Amazon forest loss increases nonlinearly with dry-season intensification. We apply a novel complex-network approach, in which Amazon forest patches are linked by observation-based atmospheric water fluxes. Our results suggest that the risk of self-amplified forest loss is reduced with increasing heterogeneity in the response of forest patches to reduced rainfall. Under dry-season Amazonian rainfall reductions, comparable to Last Glacial Maximum conditions, additional forest loss due to self-amplified effects occurs in 10-13% of the Amazon basin. Although our findings do not indicate that the projected rainfall changes for the end of the twenty-first century will lead to complete Amazon dieback, they suggest that frequent extreme drought events have the potential to destabilize large parts of the Amazon forest
Including debris cover effects in a distributed model of glacier ablation
Distributed glacier melt models generally assume that the glacier surface consists of bare exposed ice and snow. In reality, many glaciers are wholly or partially covered in layers of debris that tend to suppress ablation rates. In this paper, an existing physically based point model for the ablation of debris-covered ice is incorporated in a distributed melt model and applied to Haut Glacier dâArolla, Switzerland, which has three large patches of debris cover on its surface. The model is based on a 10 m resolution digital elevation model (DEM) of the area; each glacier pixel in the DEM is defined as either bare or debris-covered ice, and may be covered in snow that must be melted off before ice ablation is assumed to occur. Each debris-covered pixel is assigned a debris thickness value using probability distributions based on over 1000 manual thickness measurements. Locally observed meteorological data are used to run energy balance calculations in every pixel, using an approach suitable for snow, bare ice or debris-covered ice as appropriate. The use of the debris model significantly reduces the total ablation in the debris-covered areas, however the precise reduction is sensitive to the temperature extrapolation used in the model distribution because air near the debris surface tends to be slightly warmer than over bare ice. Overall results suggest that the debris patches, which cover 10% of the glacierized area, reduce total runoff from the glacierized part of the basin by up to 7%
A climate network-based index to discriminate different types of El Niño and La Niña
Funded by German Federal Ministry for Education and Research via the BMBF Young Investigators Group CoSy-CC2. Grant Number: 01LN1306A Planetary Boundary Research Network (PB.net) Earth League's EarthDoc DFG FAPESP Acknowledgments M.W. and R.V.D. have been supported by the German Federal Ministry for Education and Research via the BMBF Young Investigators Group CoSy-CC2 (grant 01LN1306A). J.F.D. thanks the Stordalen Foundation via the Planetary Boundary Research Network (PB.net) and the Earth League's EarthDoc program for financial support. J.K. acknowledges the IRTG 1740 funded by DFG and FAPESP. NCEP Reanalysis data are provided by the NOAA/OAR/ESRL PSD, Boulder, Colorado, USA, from their website http://www.esrl.noaa.gov/psd/. Parts of the analysis have been performed using the Python package pyunicorn [Donges et al., 2015b] available at https://github.com/pik-copan/pyunicorn.Peer reviewedPublisher PD
Dark Matter Direct Detection with Non-Maxwellian Velocity Structure
The velocity distribution function of dark matter particles is expected to
show significant departures from a Maxwell-Boltzmann distribution. This can
have profound effects on the predicted dark matter - nucleon scattering rates
in direct detection experiments, especially for dark matter models in which the
scattering is sensitive to the high velocity tail of the distribution, such as
inelastic dark matter (iDM) or light (few GeV) dark matter (LDM), and for
experiments that require high energy recoil events, such as many directionally
sensitive experiments. Here we determine the velocity distribution functions
from two of the highest resolution numerical simulations of Galactic dark
matter structure (Via Lactea II and GHALO), and study the effects for these
scenarios. For directional detection, we find that the observed departures from
Maxwell-Boltzmann increase the contrast of the signal and change the typical
direction of incoming DM particles. For iDM, the expected signals at direct
detection experiments are changed dramatically: the annual modulation can be
enhanced by more than a factor two, and the relative rates of DAMA compared to
CDMS can change by an order of magnitude, while those compared to CRESST can
change by a factor of two. The spectrum of the signal can also change
dramatically, with many features arising due to substructure. For LDM the
spectral effects are smaller, but changes do arise that improve the
compatibility with existing experiments. We find that the phase of the
modulation can depend upon energy, which would help discriminate against
background should it be found.Comment: 34 pages, 16 figures, submitted to JCAP. Tables of g(v_min), the
integral of f(v)/v from v_min to infinity, derived from our simulations, are
available for download at http://astro.berkeley.edu/~mqk/dmdd
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