Effect of Adenosine Receptor Agonist and Ascorbic Acid on Ultrastructure of Hippocampal CA1 Neurons after Ischemia-Reperfusion Injury

Background: In brain ischemia, blood and oxygen supply decrease and after reperfusion, free radicals and reactive oxygen species (ROS) cause severe damage. As hippocampal injury after ischemia-reperfusion causes some complications, in this study we analyzed the effect of adenosine receptor agonist (N6-cyclopentyladenosine or CPA) and ascorbic acid on ultrastructure of hippocampal CA1 neurons after ischemia-reperfusion. Methods: 35 male rats in 5 groups were used. Ischemia-reperfusion performed by occlusion of common carotids for 15 minutes. CPA and ascorbic acids were intraperitoneally injected for 7 days after ischemia, and 2 weeks before and for 7 days after ischemia, respectively. After 20 days, brain samples were isolated, prepared, and assayed using transmission electron microscopy (TEM). Findings: Ultrastructure assay of hippocampal CA1 neurons after ischemia-reperfusion with transmission electron microscopy showed recovery of intracellular organelles particularly mitochondria of treated groups. In combination therapy, these improvements were better. Conclusion: Intraperitoneal injection of CPA and ascorbic acid after ischemia-reperfusion can reduce neural damage in CA1 region of hippocampu

Temperature Shifts for Extraction and Purification of Zygomycetes Chitosan with Dilute Sulfuric Acid

The temperature-dependent hydrolysis and solubility of chitosan in sulfuric acid solutions offer the possibility for chitosan extraction from zygomycetes mycelia and separation from other cellular ingredients with high purity and high recovery. In this study, Rhizomucor pusillus biomass was initially extracted with 0.5 M NaOH at 120 °C for 20 min, leaving an alkali insoluble material (AIM) rich in chitosan. Then, the AIM was subjected to two steps treatment with 72 mM sulfuric acid at (i) room temperature for 10 min followed by (ii) 120 °C for 45 min. During the first step, phosphate of the AIM was released into the acid solution and separated from the chitosan-rich residue by centrifugation. In the second step, the residual AIM was re-suspended in fresh 72 mM sulfuric acid, heated at 120 °C and hot filtered, whereby chitosan was extracted and separated from the hot alkali and acid insoluble material (HAAIM). The chitosan was recovered from the acid solution by precipitation at lowered temperature and raised pH to 8–10. The treatment resulted in 0.34 g chitosan and 0.16 g HAAIM from each gram AIM. At the start, the AIM contained at least 17% phosphate, whereas after the purification, the corresponding phosphate content of the obtained chitosan was just 1%. The purity of this chitosan was higher than 83%. The AIM subjected directly to the treatment with hot sulfuric acid (at 120 °C for 45 min) resulted in a chitosan with a phosphate impurity of 18.5%

The comparison of flexibility, muscle strength, coordination and balance between children involve in swimming and active children / Mohd Akram Mustaqim Mohd Zamani

The purpose of this study was to compare the flexibility, strength, coordination and balance between children involve in swimming and active children. These studies are to know that activity like swimming can improve children ability or not. Children who involve in swimming which is from swimming club and active children is the kindergarten child who are not involve in swimming. A total forty children (N=40) divided by 2 group which is 20 come from swimming club children and another 20 come from kindergarten. The age of this children are around 4 to 6 years old only. This study was using one test for each of the ability. Using sit and reach test for flexibility, chair push up for strength, ball toes wall test for coordination and flamingo balance test for balance. At the end on this study showed, there is the advantage of swimming toward flexibility, strength, coordination and balance. The null hypothesis is rejected. The result of flexibility is (.000), strength (.007), coordination (.013) and last is balance (.020) for significant 2 tailed. This result proved that there is, the significant between swimming children and active children toward flexibility, strength, coordination and balance. Based on these result, children must involve in swimming and at the same time there will use their leisure time for good activity. In future study, separate the group by gender to know the difference between male and female children. As a conclusion, the swimming activity for children in 4 to 6 years old had advantage of flexibility, strength, coordination and balance

PSW statistical LSB image steganalysis

Steganography is the art and science of producing covert communications by concealing secret messages in apparently innocent media, while steganalysis is the art and science of detecting the existence of these. This manuscript proposes a novel blind statistical steganalysis technique to detect Least Significant Bit (LSB) flipping image steganography. It shows that the technique has a number of major advantages. First, a novel method of pixel color correlativity analysis in Pixel Similarity Weight (PSW). Second, filtering out image pixels according to their statistically detected suspiciousness, thereby excluding neutral pixels from the steganalysis process. Third, ranking suspicious pixels according to their statistically detected suspiciousness and determining the influence of such pixels based on the level of detected anomalies. Fourth, the capability to classify and analyze pixels in three pixel classes of flat, smooth and edgy, thereby enhancing the sensitivity of the steganalysis. Fifth, achieving an extremely high efficiency level of 98.049% in detecting 0.25bpp stego images with only a single dimension analysis

Is 8860 variation a rare polymorphism or associated as a secondary effect in HCM disease?

Introduction: mtDNA defects, both deletions and point mutations, have been associated with hypertrophic cardiomyopathies. The aim of this study was to establish a spectrum for mtDNA mutations in Iranian hypertrophic cardiomyopathy (HCM) patients. Material and method: The control group was chosen among the special medical centre visitors who did not have hypertrophic cardiomyopathy or any related heart disease. Hypertrophic cardiomyopathy (HCM) is widely accepted as a pluricausal or multifactorial disease. Because of the linkage between energy metabolism in the mitochondria and cardiac muscle contraction, it is reasonable to assume that mitochondrial abnormalities may be responsible for some forms of HCM. Point mutations and deletions in the two hot spot regions of mtDNA were investigated by PCR and sequencing methods. Results: Some unreported point mutations have been found in this study but no deletion was detected. Meanwhile some of these point mutations have been investigated among HCM patients for the first time. Conclusions: A8860G transition was detected in a high proportion, raising the question whether this rare polymorphism is associated as a secondary effect in HCM disease. Copyright © 2011 Termedia & Banach

Instructing anger management skills for mothers of mentally retarded children: effects on mother-child relationship

AbstractThe purpose of the present research is to examine the effects of anger management training on mother's relationship with their educable mentally retarded and slow learner children. The design of this study is quasi-experimental with pretest-posttest and control group. This study was conducted on 46 mothers who were assigned equally into experimental and control groups. The anger management training was implemented on experimental group in seven sessions, 2hours each. Data were collected utilizing Anger Evaluation Scale and Multidimensional Anger Inventory. An analysis of covariance was used for this research. Findings showed that intensity and frequency of anger decreased and the use of anger control strategies increased, indicating the effectiveness of anger management techniques

A secure audio steganography approach

A wide range of steganography techniques has been described in this paper. Beside the evaluation of embedding parameters for the existing techniques, two problems -weaknesses- of substitution techniques are investigated which if they could be solved, the large capacity - strength- of substitution techniques would be practical. Furthermore, a novel, principled approach to resolve the problems is presented. Using the proposed genetic algorithm, message bits are embedded into multiple, vague and higher LSB layers, resulting in increased robustness

The effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring

BACKGROUND AND OBJECTIVE: Celery (Apium graveolens), in traditional medicine is known as appetite and libido stimulant, and also there has been referred to its effect on miscarriage and catamenia. This plant with known plant compounds could be an option for fertility control. This study was conducted to evaluate the effect of hydro-alcoholic extract of celery on male rats in fertility control and sex ratio of rat offspring. METHODS: In this experimental study, 20 male rats were divided randomly into 4 groups, 5 in each group. Control group received no treatment; Sham group received 1 ml normal saline and the treatment groups 1 and 2 received hydro-alcoholic extract of celery in doses of 100 and 200 mg/Kg/BW respectively for 5 weeks. After mating each male rat with two female rats, the number of delivered mice and the number of offspring and their sex were recorded and compared 30 days after the birth. FINDINGS: There was no significance difference between the experimental and control group (p>0.05). The result showed a significant decrease in the number of offspring in doses of 100 and 200 mg/kg (4.25±0.25 and 4.50±0.32 respectively) compared to the control group (7.11±0.26), also a significant increase in the sex ratio in dose of 100 mg/kg (2.93±0.30) compared to the control group (0.98±0.11) was observed (p<0.05). CONCLUSION: It seems that consumption of celery in male decreases the number of offspring and increases the male genesis; because it does not affect on childbirth. It may be useful safely in pregnancy controlling. © 2014, Babol University of Medical Sciences. All rights reserved

The effect of Halcoholic extract of celery leaves on the delivery rate (fertilization and stillbirths), the number, weight and sex ratio of rat off spring

Objectives: Infertility is one of the most common health problems in the world. Different plants are used in traditional medicine to treat infertility. Celery is rich in flavonoids and vitamins E and C. The present study investigated the effect of hydroalcoholic extract of celery leaves on the delivery rate, number, weight and sex ratio of rat offspring. Materials and Methods: In this experimental study, 45 Wistar rats were randomly divided into three groups of fifteen (five male and 10 female rats per group). The control group received 1 ml of distilled water, and experimental groups received respectively doses of 100 and 200 mg/kg/BW celery extract for five weeks by gavage. During the fifth week each male rat was mated with two female rats from the same group in a separate cage. After a week, every female rat was kept in the separate cages. Females received celery extract during pregnancy and until childbirth. Finally, the number of the delivery females, number, weight and sex ratio of offspring were recorded. The data were analyzed by one-way ANOVA test using SPSS15 software. Results: The number of the delivery rats in the experimental groups was not significantly different than the control group (P>0.05). The number of newborns was significantly increased in each experimental group compared to the control group (P0.05). Conclusions: Celery improved fertility due to have flavonoid and antioxidant activity, and its consumption in both sexes can be useful in the treatment of infertility. © 2014 AENSI Publisher All rights reserved