Virološko i molekularno istraživanje na uzorcima bizona pod sumnjom da su zaraženi virusom bizonskih boginja u Egiptu 2019. godine

In this study, skin lesions from buffaloes showing clinical signs of buffalopox infection were tested to isolate and identify the buffalopox virus (BPXV). Clinical examination of infected buffaloes was performed and visible clinical signs recorded. Skin scabs from infected buffaloes were collected and used for virus isolation on embryonated chicken egg (ECE) and tissue culture cell lines. The isolated BPXV was identified and characterized using polymerase chain reaction (PCR). The infected buffaloes displayed fever, skin eruptions, enlargement of superficial lymph nodes, emaciation and drop in milk yield. The ECE inoculated with the prepared skin scab samples showed clear raised white pock lesions on the chorioallantoic membrane (CAM). The inoculated tissue cultures (VERO and BHK cell lines) revealed a cytopathic effect (CPE) including rounding, clumping with cytoplasmic granulation and cluster formation. PCR for the C18L specific BPXV gene was carried out on the virus infected tissue culture produced 368 bp bands. Human infection with BPXV was also recorded. It was concluded that BPXV is circulating in Egyptian buffaloes, causing economical losses and infection in contact humans.U ovoj studiji lezije kože bizona koje su pokazivale kliničke znakove infekcije bizonskim boginjama, rabljene su za izoliranje i identifikaciju virusa bizonskih boginja (BPXV). Provedeno je kliničko ispitivanje zaraženih bizona i zabilježeni su očiti klinički znaci. Kraste s kože zaraženih bizona su prikupljene i rabljene za izolaciju virusa na embrioniranom kokošjem jajetu (ECE) te linijama tkivnih staničnih kultura. Izolirani BPXV identificiran je i okarakteriziran uporabom lančane reakcije polimerazom (PCR). Zaraženi bizoni imali su vrućicu, osip na koži, povećanje površinskih limfnih čvorova, mršavjeli su, a i došlo je do pada proizvodnje mlijeka. ECE cijepljeni pripremljenim uzorcima krasta s kože pokazali su jasne uzdignute bijele lezije boginja na korioalantoičnoj membrani (CAM). Cijepljene tkivne kulture (VERO i BHK stanične linije) otkrile su citopatski učinak (CPE), uključujući zaobljivanje, agregaciju s granulacijom citoplazme i formiranje klastera. PCR za C18L specifični gen virusa bizonskih boginja proveden je na tkivnoj kulturi zaraženoj virusom proizvevši 368 bp vrpci. Zabilježena je i zaraza ljudi BPXV-om. Zaključeno je da BPXV kruži među egipatskim bizonima, što prouzroči, dođu li u kontakt s BPXV zarazu ljudi i velike ekonomske gubitke

Regional analysis using the Geomorphoclimatic Instantaneous Unit Hydrograph

International audienceThe construction of design flood hydrographs for ungauged drainage areas has traditionally been approached by regionalisation, i.e. the transfer of information from the gauged to the ungauged catchments in a region. Such approaches invariably depend upon the use of multiple linear regression analysis to relate unit hydrograph parameters to catchment characteristics and generalised rainfall statistics. The concept of the geomorphological instantaneous unit hydrograph (GIUH), in relating the shape and scale of the catchment transfer function to stream network topology and channel characteristics, offers an alternative methodology. GIUH derivation depends upon a series of assumptions, including that of estimating a "characteristic velocity"; these continue to attract attention and debate. However, if this velocity is expressed in terms of the kinematic wave approximation, the peak and time-to-peak of the IUH may be expressed in terms of a group of catchment and channel characteristics and the intensity of rainfall excess, giving the so-called geomorphoclimatic IUH (GCIUH). Previous studies involving the GCIUH have developed a single IUH relating to the total duration of rainfall excess. In this study, the rainfall excess duration was divided into several (equal) time increments, with separate IUHs being generated for each interval. This quasi-linear approach was applied to 105 storm events from nine catchments in the south-west of England, ranging in size from 6 to 420 (km)2 . The results showed that, providing the time interval chosen is fine enough to capture the shape of the runoff hydrographs, a comparable level of goodness-of-fit can be obtained for catchments covering a range of about 1:75 in area. The modified GCIUH approach as described is therefore recommended for further investigation and intercomparison with regression-based regionalisation methods. Keywords: floods; geomorphology; rainfall-runoff modellin

High-precision calculations of In I and Sn II atomic properties

We use all-order relativistic many-body perturbation theory to study 5s^2 nl configurations of In I and Sn II. Energies, E1-amplitudes, and hyperfine constants are calculated using all-order method, which accounts for single and double excitations of the Dirac-Fock wave functions.Comment: 10 pages, accepted to PRA; v2: Introduction changed, references adde

Analisis Struktur Dan Nilai-nilai Moral Yang Terkandung Dalam Cerpen Ten Made Todoke Karya Yoshida Genjiro

The sequence of this thesis are: first the writer collects the references and analyzes the data. Then, she obtained the data from Anthology of Japanese children's literature's book. The problems that will be studied in this thesis are intrinsicelements and the moral values contained in Ten Made Todoke Yoshida Genjiro's short story. This study uses structural method. The method is used to analyze the intrinsicelements that lead to moral values of the story. It makes the writer easier to analyze the moral values of story. The intrinsic elements that will be discussed in this thesis are theme, character, plot, setting, point of view, and the message of story

Beyond the simple Proximity Force Approximation: geometrical effects on the non-retarded Casimir interaction

We study the geometrical corrections to the simple Proximity Force Approximation for the non-retarded Casimir force. We present analytical results for the force between objects of various shapes and substrates, and between pairs of objects. We compare the results to those from more exact numerical calculations. We treat spheres, spheroids, cylinders, cubes, cones, and wings; the analytical PFA results together with the geometrical correction factors are summarized in a table.Comment: 18 pages, 19 figures, 1 tabl

Modulatory role of chelating agents in diet-induced hypercholesterolemia in rats

AbstractIntroductionHypercholesterolemia is a major risk factor for the development of atherosclerosis and endothelial dysfunction. Chelating agents may play a modulatory role in atherosclerosis by removal of calcium from atherosclerotic plaques.AimThe present study aimed to explore the effects of calcium disodium ethylenediaminetetraacetic acid (CaNa2EDTA) and meso-2,3-dimercaptosuccinic acid (DMSA) on diet-induced hypercholesterolemia in rats using simvastatin as a reference standard.MethodsHypercholesterolemia was induced by feeding rats with cholesterol-rich diet for six weeks. Rats were divided into five groups (n=8): normal control, hypercholesterolemic control, simvastatin (20mg/kg; p.o.), CaNa2EDTA (100mg/kg; i.p.) and DMSA (100mg/kg; i.p.). Treatments continued daily for the six weeks of diet feeding.ResultsDiet-induced hypercholesterolemia resulted in alterations in the lipid profile markers and a state of oxidative stress coupled by compensatory increase in serum nitric oxide (NO) level and decreased aortic endothelial nitric oxide synthase (eNOS) activity parallel to increased inducible nitric oxide synthase (iNOS) activity, aortic calcium content and aortic wall thickness. Treatment with simvastatin, CaNa2EDTA and DMSA improved lipid profile and oxidative stress markers. In addition, they attenuated hypercholesterolemia-induced changes in serum NO level, aortic eNOS and iNOS activities, calcium content and aortic wall thickness.ConclusionPretreatment of hypercholesterolemic rats with simvastatin, CaNa2EDTA or DMSA attenuated most of the changes induced by feeding rats with cholesterol-rich diet owing to their observed anti-hyperlipidemic and antioxidant properties