Application of Formative Assessment to Measure Students' Self-Regulation in Physics Lessons

In the world of education, formative assessment is critical because it is to know the growth of students when doing a lesson and get an idea regarding the way the teacher develops learning methods that occur. The purpose of the study was to determine the application of formative assessment to measure students' self-regulation. This study's data collection methods used questionnaires and test questions with data analysis techniques using qualitative descriptive. Respondents in this study amounted to 35 students of class XI IPA 4 SMA Negeri 14 Surabaya. The results showed that self-regulation or the ability to regulate oneself in students could be known and measured using the application of formative assessment. The self-assessment results on students are more striking when measured using a questionnaire. They can use seven categories of self-regulation to assess and observe their expertise, skills, competencies, and performance presented in the form of a questionnaire. Self-regulation ability that can measure is undoubtedly beneficial for the future orientation of students in motivating and controlling their learning process. In addition, with the application of formative assessment, teachers get feedback on the learning process that is being developed to monitor the progress and growth of students during the learning process

Analysis of MeEf1A6 Gene Promoter Activity with In-vitro and In-vivo using Transient and Stable Expression Techniques in Tobacco Plant (Nicotiana tabacum)

The promoter is a part of the gene that functions in carrying out the gene expression, and its work activity becomes a matter of concern to ensure that expression works effectively. MeEF1A6 (Manihot esculenta Elongation Factor 1 Alfa - 6) is a promoter derived from cassava plants (Manihot esculenta). In previous studies, the MeEF1A6 promoter was successfully isolated, introduced, and characterized into the pBI121 plasmid, replacing the CaMV35S promoter. This study aims to analyze the activity of MeEF1A6 promoters in-vivo and in-vitro by using transient and transgenic techniques in tobacco plants. The pBI121 plasmid containing the MeEF1A6 promoter was introduced into Agrobacterium tumefaciens strain AGL1 and LBA4404. The promoter's work was then analyzed by the result of introducing it into the tobacco plant using the transient and stable transformation. The whole part of explants was used for transient study and tested in a minimum of two biological replicates. Sixty sheets of explant leaves that have been cut were used for stable transformation. The promoter work analysis was carried out with the GUS gene expression that integrated with the promoter with histochemical GUS assay. The transient produced a blue color in the roots, stems, and leaves on the whole repetition. The transverse incision in the stem shows the blue color on the epidermis and procambium tissue. Stable transformation using AGL1 as vector produced 43 shoots from 40 calli. A total of 43 shoots were selected with antibiotics and produced 27 plantlets that were successfully grown. Some plantlets are then reacted with x-gluc as histochemical GUS assay substrat and produced a blue color in the explants, indicating that the MeEF1A6 promoter has been successfully introduced. The results indicate that the MeEF1A6 promoter could work on plant tissue in roots, stems, leaves, and tissues that connect meristems such as procambium in tobacco plants. This reinforces the suspicion that the MeEF1A6 promoter performs work constitutionally as a constitutive promoter. 

Pengaruh Pemijatan Perineum pada Primigravida Terhadap Pencegahan Ruptur Perineum di UPT Puskesmas Kassi-Kassi Makassar Tahun 2020

xii, 108 hlm.; 28 cm

Proteomics of model and crop plant species: status, current limitations and strategic advances for crop improvement.

In the last decade proteomics studies have gained increasing importance in plant research. The development of proteomics techniques allowing increased proteome coverage and quantitative measurements of proteins have been particularly instrumental to characterize proteomes and their modulation during plant development, biotic and abiotic stresses. Despite important advances, plant proteome analysis, including those of model plant species, remain constrained by limitations inherent to proteomics techniques and data interpretation. Here we review the approaches and achievements of proteomics with model plant and crop species (i.e. Arabidopsis and rice) and discuss the current limitations of crop proteomics. We anticipate future directions that could advance the contribution of plant proteomics to crop improvement

Robust transformation procedure for the production of transgenic farmer-preferred cassava landraces

<p>Abstract</p> <p>Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly for the cassava model cultivar 60444 because of its good regeneration capacity of embryogenic tissues. However, for deployment and adoption of transgenic cassava in the field it is important to develop robust transformation methods for farmer- and industry-preferred landraces and cultivars. Because dynamics of multiplication and regeneration of embryogenic tissues differ between cassava genotypes, it was necessary to adapt the efficient cv. 60444 transformation protocol to genotypes that are more recalcitrant to transformation. Here we demonstrate that an improved cassava transformation protocol for cv. 60444 could be successfully modified for production of transgenic farmer-preferred cassava landraces. The modified transformation method reports on procedures for optimization and is likely transferable to other cassava genotypes reportedly recalcitrant to transformation provided production of high quality FEC. Because the three farmer-preferred cassava landraces selected in this study have been identified as resistant or tolerant to cassava mosaic disease (CMD), the adapted protocol will be essential to mobilize improved traits into cassava genotypes suitable for regions where CMD limits production.</p

A method for rapid and homogenous initiation of post-harvest physiological deterioration in cassava storage roots identifies Indonesian cultivars with improved shelf-life performance

Abstract Cassava is the most cultivated and consumed root crop in the world. One of the major constraints to the cassava value chain is the short shelf life of cassava storage roots which is primarily due to the so-called post-harvest physiological deterioration (PPD). The identification of natural sources of PPD tolerance represents a key approach to mitigating PPD losses by generating farmer- and industry-preferred cassava cultivars with prolonged shelf life. In the present study, a PPD assessment method was developed to screen for PPD tolerance in the cassava germplasm. The proposed PPD assessment method displayed a reduced rate of microbial infection and allowed a rapid and homogenous development of typical PPD symptoms in the cassava storage roots. We successfully used the PPD assessment method in combination with an image-based PPD scoring method to identify and characterize PPD tolerance in 28 cassava cultivars from the Indonesian cassava germplasm. Our analysis showed a significant and positive correlation between PPD score and dry matter content (r = 0.589–0.664, p-value < 0.001). Analysis of additional root parameters showed a significant and positive correlation between PPD scores at 2 days post-harvest (dph) and root length (r = 0.388, p-value < 0.05). Our analysis identified at least 4 cultivars displaying a significantly delayed onset of PPD symptoms as compared to the other selected cultivars. The availability of cassava cultivars contrasting for tolerance to PPD will be particularly instrumental to understanding the molecular mechanisms associated with delayed PPD in cassava roots

UKM medical graduates’ perception of their communication skills during housemanship

The art of talking to patients and their relatives does not come naturally to most of us and the ability to put oneself in the patients’ predicament is difficult particularly for the young doctors. To identify the communication abilities of the young doctors, a cross sectional study was carried out on 32 house officers who graduated from UKM in 2004 during their house jobs at different hospitals in Malaysia. A standardized questionnaire was used to collect the data. Fifty nine percent respondents claimed that they had communicated very well with patients while 69% with support staff and 88% with peers. On the other hand 38% and 41% of the respondents claimed they communicated very well with their superiors and families of patients. Only 22% of the graduates’ skills of communication in breaking bad news were very well, while 50% and 81% were very well in counselling patients and taking consent for procedures. Curriculum planners need to emphasize the importance of developing good communication skills in all aspects for the future doctor

Cassava post-harvest physiological deterioration: From triggers to symptoms

The production of cassava, the most important staple root crop in the world, is constrained by the short shelf life of the cassava storage roots that are undergoing post-harvest physiological deterioration (PPD) shortly after harvest. PPD reduces starch quality and renders the roots unpalatable and unmarketable. PPD is a complex process involving enzymatic stress responses to wounding, changes in gene expression and protein synthesis as well as accumulation of secondary metabolites. PPD can be strongly influenced by environmental factors making the identification of genotypes with delayed PPD trait difficult. In the present review, we propose an integrative presentation of PPD phenomenon based on a comprehensive analysis of several key PPD studies. We discuss recent progress in the standardization of methods to assess and score PPD tolerance in cassava roots. Traditional and improved storage techniques to extend cassava shelf-life are presented and prospects of transgenic approaches to delay PPD are discussed

The influence of Prandtl number on near-wall turbulent heat transfer

Za opis turbulentnega prenosa toplote iz stene na tekočino je pri nizkih vrednostih Reynoldsovih in Prandtlovih številih mogoče uporabiti neposredno numerično simulacijo (NNS-DNS), ki opiše vse krajevne in časovne skale pojava.Vpliv Reynoldsovega števila na turbulentni prenos toplote (hitrosti, temperature, fluktuacije itn.) je razmeroma majhen, medtem ko je vpliv Prandtlovega števila veliko večji, in sicer Pr=0.025, Pr=1 in Pr=5.4. Ločljivost NNS turbulentnega prenosa gibalne količine je premo sorazmerna z Re 3/4 v vseh smereh koordinatnega sistema. Pri upoštevanju prenosa toplote, za tekočine s Prandtlovim številom, večjim od ena, velja, da je ločljivost premo sorazmerna z Re 3/4Pr 1/2. Pri Re=5260 in Pr=5.4 smo opravili tri numerične simulacije pri različnih ločljivostih. Vse tri simulacije so NNS za hitrostno polje , samo simulacija z največjo ločljivostjo je tudi NNS za temperaturno polje. Rezultati so pokazali, da je mogoče temperaturno polje zelo natančno napovedati tudi z nekoliko slabšo ločljivostjo od teoretično zahtevane.For describing the heat transfer from a wall to a fluid at low Reynolds and Prandtl numbers we can use a direct numerical simulations (DNS), which describes all the length and time scales of the phenomenon. The Reynolds number has a weak influence on the turbulent heat transfer (velocities, temperatures, RMS-fluctuations ...), where as the increasing Prandtl number has a stronger influence. In our flow simulations in the channel, three different Prandtl numbers, i.e. Pr=0.025, Pr=1 and Pr=5.4, et a Reynolds number=5000 were analyzed. The resolution of the DNS for turbulent momentum transfer is proportional to Re 3/4 in all directions. When considering heat transfer in fluids for a Prandtl number higher than one, the resolution is proportional to Re 3/4Pr 1/2. Three diferent numerical simulations at different resolutions were performed at Re=5260 and Pr=5.4. All three simulations are a DNS for the velocity field,whereas only the simulation at the highest resolution is also a DNS for the thermal field. The results showed that the thermal field could be accurately described with a lower resolution than theoretically required